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식육 중 잔류물질 스크리닝법인 EEC-4 Plate Test와 Charm II Test 비교
함희진,김두환,권택부,이정학,Ham Hee-jin,Kim Doo-whan,Kweon Taek-boo,Lee Jung-hark 한국식품위생안전성학회 2004 한국식품위생안전성학회지 Vol.19 No.4
잔류물질 간이 시험법으로 EEC-4 plate test와 Charm II test가 항생물질과 합성항균제 잔류검사에 널리 이용되고 있는 바, 이들 상호간의 결과 수치를 비교, 분석한 결과 다음과 같은 결론을 얻었다. EEC-4 plate혹은 Charm II 에서 양성으로 나타난 돈육 71건에 대하여 HPLC로 정량 분석한 결과, only tetracyclines 46건, only sulfonamides와 solfonamides+tetracyclines가 각각 4건씩, amphenicols+tetracyclines와 amphenicols+sulfonamides+tetracyclines가 각각 1건씩 검출되었고, 15건에서는 검출되지 않았다. Sulfonamides 계열에서 검출된 9건의 경우, Charm-II test 결과 수치가 낮은 경우에 정량 검출량이 높게 나타났고, EEC-4 plate test 결과 pH 6.0, pH 7.2, pH 8.0 모두에서 양성으로 나타나는 등 두 시험방법 간에 연관성이 있었고, chloramphenicols 계열에서 검출된 2건과 검출되지 않은 7건의 경우, Charm-II test결과 수치가 낮은 경우에는 오히려 정량 검출되지 않았으나, control point 근방의 양성 수치에서는 검출량이 높게 나타날 뿐 아니라 EEC-4 plate test에서도 pH6.0, pH7.2, pH8.0 모두에서 양성으로 나타나는 등 두 시험방법 간에 연관성이 있었다. 한편, tetracycline계열의 경우, EEC-4 plate와 Cham-II간에 연관성은 없었다. 또한, EEC-4 plate와 Charm II에서 각각 양성을 나타내었으나 HPLC에서는 검출되지 않는 경우는 tetracyclines계열에서는 $10.5\%(6/57)$, sulfonamides계열의 경우는 없었고, chloramphenicols계열의 경우는 $81.8\%(9/11)$로 각각 나타나 EEC-4 plate와 Charm II가 세 가지 잔류물질 시험에 대한 screening method로서 적합함을 알 수 있었다. It was carried out to compare the relationship of EEC 4-plate test method and Charm II test method by HPLC. 46 only tetracyclines, 4 only sulfonamides, 4 sulfonamides+tetracyclines, one amphenicols+tetracyclines, and one amphenicols+sulfonarnides+tetracyclines, it was detected on 71 porks of EEC 4-plate positive or Charm II positive by HPLC. In case of sulfonamides and chloramphenicols, two test methods had related with each other, but had no relation in tetracyclines. Also, it was $10.5\%(6/57)$ tetracyclines, $81.8\%(9/11)$ chloramphenicols that were EEC 4 plate positive or Charm II positive, but not detectrd by HPLC. Consequently, EEC 4-plate test method and Charm II test method were suitable by screening test on antibiotics and sulfonamides etc.
시판 햄류, 소시지류, 건조저장육류 중 소르빈산 함량조사
함희진,양윤모,윤은선 한국식품위생안전성학회 2003 한국식품위생안전성학회지 Vol.18 No.1
2002년 1-12월까지 시험 의뢰된 식육가공품에 대하여 소르빈산 함량을 시험, 조사한 결과, 14.9%(67/450)에서 소르빈산이 검출되었고, 유형별로는 건조저장육 65.6%(21/32), 베이컨 17.6%(3/17), 햄 11.7%(27/231), 소시지 11.7%(13/111), 기타식육가공품(통조림 등) 11.5%(3/26) 순이었으며, 소르빈산 함량 범위는 ND-1.21 g/Kg, 평균 0.86 g/Kg이었는데, 유형ㅂ려로는 각각, 건조저장육 ND-1.21 g/Kg, 0.34 g/Kg, 베이컨 ND-0.84 g/Kg, 0.12 g/Kg, 햄 ND-1.27 g/Kg, 0.074 g/Kg, 소시지 ND-0.90 g/Kg, 0.077 g/Kg 그리고 기타식육가공품(통조림 등) ND-0.20 g/Kg, 0.015 g/Kg 등으로 나타났다. 결국, 식품의 안전성과 위생적인 식품 보급을 위해서는 소리빈산 등 보존료에 대한 지속적인 감시가 필요함을 알 수 있었다. This study was performed to investigate the contents of nitrites in 450 meat products in Seoul from Jan. to Dec. in 2002. Sorbic acid contents of the samples were determined by Gas Chromatography. 67 samples of 450(14.9%) were detected in sorbic acid contents. In each meat products, 65.6%(21/32) in dried meats, 17.6%(3/17) in bacon, 11.7%(27/231) in hams, 11.7%(13/111) in sausages, and 11.5%(3/26) in meat can. The concentration range of Sorbic acid and its average content in each of the meat products are as follows: ND-1.21 g/Kg and 0.34 g/Kg in dried meat, ND-0.84 g/Kg and 0.12 g/Kg in bacon, ND-1.27 g/Kg and 0.074 g/Kg in ham, ND-0.90 g/Kg and 0.077 g/Kg in sausage, and ND-0.20 g/Kg, 0.015 g/Kg in can meat. Together, these results demonstrated that the processed market meat products must be reinforced to supply for the citizens as safe foods.
함희진,오세아,조석주,최성민 대한미생물학회 2014 Journal of Bacteriology and Virology Vol.44 No.1
Antigen distributions of rotavirus and adenovirus were detected by enzyme immuno assay (EIA) in patients with acute gastroenteritis in Seoul from 2008 to 2012. Tested feces sample numbers from patients were 9,597. Antigen distributions of rotavirus and adenovirus were 13.9%, and 2.1%, respectively. The patterns of each viral pathogen may give potentially effective data for epidemiological studies.
함희진 대한미생물학회 2017 Journal of Bacteriology and Virology Vol.47 No.4
The study was conducted to investigate the distribution of pathogenic bacteria related to agricultural herb products that are sold on the market in South Korea. A survey was conducted on the microbial contamination levels and antibiotic susceptibility of Bacillus cereus (B. cereus) among 194 agricultural herb products on sale in Seoul. Distributions of those isolates were 252 coliforms, 148 Bacillus spp., 75 Enterococcus spp., 10 Staphylococcus spp., and 6 Listeria spp., respectively. The number of B. cereus isolates was 34, Escherichia coli isolates was three, Enterococcus faecium isolate was one, and Enterococcus faecalis isolate was one. Antibiotic susceptibility of B. cereus isolates was tested against 36 kinds of antibiotic susceptibility discs by disc diffusion method. B. cereus isolates were resistant to 20 kinds of antibiotics and semi-resistant to 11 kinds of antibiotics. On the basis of these results, any agricultural herb product can be assumed to be resistant or semi-resistant to the antibiotics used in human. In conclusion, we suggest sanitary control and special management regarding B. cereus contamination in agricultural herb products.
함희진 한국어학회 2007 한국어학 Vol.34 No.-
In this study, the two arguments on the status of {ttuli-} are compared: One regards {ttuli-} as an affix and the other regards it as a verb. It is suggested here that {ttuli-} is the second part of a compound verb. This suggestion can be supported by two observations on {ttuli-} compound verbs in Contemporary Korean. The first observation indicates that {ttuli-} is a transitive verb that existed before the period of Contemporary Korean. {ttuli-} is assumed to have a meaning of 〈falling〉, 〈detachment〉, 〈decrease〉 and can be analyzed as '^(*)ttul-+-i-(causative affix)'. The second observation show that {ttuli-} originated from the causative form of '^(*)tut-', a synonym of 'ti-' in Middle Korean. These support my claim that {ttuli-} is a root.