시비처리에 따른 소나무의 내건성 반응특성 연구

박진화,이도형 경상대학교 농업생명과학연구원 2020 농업생명과학연구 Vol.54 No.2

본 연구는 소나무 2-0 용기묘를 대상으로 시비처리에 따른 생장변화와 시비처리 후 단수처리에 따른 생리활성 측정을 통해 내건성 증진을 위한 적정시비 방안을 수립하여 건강한 묘목 생산을 위한 기초자료를 제공하고자 수행되었다. 시비처리에 따른 소나무 묘목은 중도(2.0g/ℓ)의 시비구에서 가장 높은 묘고 생장량(12.34±0.73㎝)을 나타냈으며 근원경 생장은 약도(1.0g/ℓ)의 시비구에서 가장 높은 생장량(5.77±0.20㎜)을 나타냈다. 묘목의 품질을 평가하는 종합적 지수인 묘목품질지수(SQI)는 약도(1.0g/ℓ)의 시비구에서 2.05±0.07로 가장 높게 나타났으며, 중도(2.0g/ ℓ)의 시비처리구에서는 1.90±0.09의 값을 보였다. 전체 엽록소 함량은 시비 농도가 높아짐에 따라 증가하는 경향을 보였고 강도(3.0g/ℓ)의 시비구에 서 22.50±0.28㎎/g으로 가장 높게 나타났다. 단수처리에 따른 시비구별 소나무 묘목의 아브시스산 함량은 엽 피해가 발생한 강도(3.0g/ℓ)의 시비구를 제외하면 중도(2.0g/ℓ)의 시비구에서 2,190.85ng/g로 가장 낮게 나타났고, 전체 엽록소 함량은 중도(2.0g/ℓ)의 시비구에서 건조스트레스에 따른 엽록소의 파괴가 거의 없이, 단수처리 기간 동안 꾸준히 가장 높은 엽록소 함량 값(21.20±0.20㎎/g)을 나타냈다. 공시 수종인 소나무는 중도(2.0g/ℓ) 의 시비구에서 건조스트레스에 따른 아브시스산의 함량이 가장 적고, 엽록소의 함량은 가장 높게 나타나 소나무의 내건성 증진을 위한 적정 시비수준 은 중도(2.0g/ℓ)라고 판단된다. The main aim of this study is first to measure changes in the growth of 2-year-old Pinus densiflora containerized seedlings as influenced by fertilizer treatments and water supply cut-off proxy of drought resistance, second to establish a proper fertilization plan for the promotion of drought resistance, and finally to provide basic data for healthy seeding production. For the fertilization treatments, the height growth of P. densiflora seedlings was 12.34±0.73cm at an intermediate (2.0g/ℓ) fertilizer treatment, while the diameter growth of root collar was 5.77±0.20mm at a weak (1.0g/ℓ) fertilizer treatment. The highest seedling quality index of 2.05±0.07 was shown at a weak (1.0g/ℓ) fertilizer treatment. The total chlorophyll content increased as the fertilizer concentration increased, and the highest total chlorophyll content of 22.50±0.28 ㎎/g was observed at a strong (3.0g/ℓ) fertilizer treatment and fallowed total chlorophyll content of 1.90±0.09 ㎎/g was found at an intermediate (2.0g/ℓ) fertilizer treatment. For the abscisic acid content of P. densiflora seedlings for each fertilizer treatment during water supply cut-off, the lowest abscisic acid content of 2,190.85ng/g was found at an intermediate (2.0g/ℓ) fertilizer treatment. Exceptionally, seedlings with strong (3.0g/ℓ) fertilizer treatment showed poor growth evident by damaged leaves. Besides, the highest total chlorophyll content of (21.20±0.20㎎/g) was found at an intermediate (2.0g/ℓ) fertilizer treatment constantly during the period of water supply cut-off without destruction of chlorophyll by the drought stress. In conclusion, P. densiflora seedlings showed the lowest abscisic acid content and the highest chlorophyll content during water cut-off or drought stress at the intermediate (2.0g/ℓ) fertilizer treatment. The results suggested that the appropriate fertilizer treatment for improving the drought resistance of P. densiflora seedlings is the intermediate (2.0g/ℓ) level.

Identify the Effect of Fertilizer on the Fluorescence Color Distribution of Carrot during Growing Period

( Mohammad Miah ),( Dimas Riza ),( Tetsuhito Suzuki ),( Naoshi Kondo ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

The aim of this research was to monitor the fluorescence characteristic of carrot during the growing period with different fertilizer treatment which will enable to develop a method for tracking the harvested carrot fertilizer treatment. Five treatments were selected in this research, i.e., chemical fertilizer(C); organic fertilizer (O); mix of chemical and organic (CO) fertilizer; mix chemical plus organic fertilizer plus lime (COL); and control(S). The cultivar was “kouten nigou “and three sampling periods were carried out at 56, 87, and 117 days after seeding (DAS). Optical information by means of fluorescence compound color information has been obtained from both vertically cut carrot surface and the outer surface. Two types of the image were taken, a) color image using halogen light source, b) fluorescence image was taken using 365 nm LED. The Blue/Red (B/R) ratio was found the maximum in the taproot of carrot then strip> middle> outer layer> inner layer. The treatment of CO, COL, and O showed the highest B/R ratio in all part of carrot compared to other treatments. But the outer surface of these treatments showed opposite that means more reddish color compared with other treatments. Besides, it was found that CO, COL, and O treatments cover highest bluish color area measured in term of total pixels count. In addition, a principal component analysis (PCA) has been used to explore the data structure of each treatment of fertilizer. It was found that it is possible to identify the treatment of fertilizer applied during the growing period of carrot. These results showed that fluorescence imaging system has a potentiality to identify the fertilizer treatment of harvested carrot.

SCOD_Mn 농도 및 pH 조정이 양돈분뇨의 후숙발효과정에 미치는 이화학적 영향

홍인기,김수량,이명규,Hong, In-Gi,Kim, Soo-Ryang,Lee, Myung-Gyu 한국축산환경학회 2012 축산시설환경학회지 Vol.18 No.suppl.

연구는 양돈분뇨의 액비화 처리과정 중 농지환원 직전단계인 액비 후숙처리 단계에 있어서 $SCOD_{Mn}$ 농도 및 산도(pH)의 조정이 후숙액비의 이화학적성상에 미치는 영향을 알아보고 적절한 후숙 액비화 공정을 구축하기 위하여 수행하였으며, 실험결과를 요약하면 다음과 같다. 1. $SCOD_{Mn}$ 감소율은 알칼리발효 처리구에서 평균 29.9%, 인산중성화 처리구에서는 평균 36.9%로 인산 중성화 처리구가 비교적 높은 감소율을 보였다. 2. 30일간 처리 후 $SCOD_{Mn}$의 농도가 가장 낮은 처리구는 유입농도가 가장 낮았던 인산 중성화 처리구 T-6이다. 후숙 액비의 목표수준을 $SCOD_{Mn}$ 3,000 ppm 이하로 가정할 경우 후숙 발효조의 초기 투입농도는 5,500 ppm 이하로 조정하는 것이 적합하다고 사료된다. 3. 질소의 잔존율은 알칼리발효 처리구에서 평균 29.3%, 인산중성화 처리구에서 평균 38.9%로 인산중성화 처리구가 비교적 질소의 손실이 적은 한편, T-P의 경우 인산중성화 처리구에서 높은 농도(평균 1,473 ppm)로 유지되었다. 4. 본 연구를 통해 액비 후숙처리 시 '저인산-저질소'의 형태는 "알칼리발효 처리구", '고인산-고질소'의 형태는 "인산중성화 처리구"의 조건이 유리할 것으로 기대된다. This research investigated the effect of $SCOD_{Mn}$ concentrations and pH adjustment at the stage before land application, namely 2nd-aeration treatment stage of liquid fertilizer in the liquid fertilizer treatment process of swine manure on the physicochemical compositions of 2nd-aeration treated liquid fertilizer. The liquid fertilizer used in this research is the alkaline fermented liquid fertilizer of swine manure more than pH 9.0 through aeration treatment (Alkaline fermentation treatment group). About the alkaline liquid fertilizer, phosphate neutralization treatment was conducted with phosphoric acid and it was a phosphate neutralization treatment group. In 2nd-aeration treatment of liquid fertilizer for 30 days, each group was divided into alkaline treatment groups (T-1, T-2, and T-3) and phosphate neutralization treatment groups (T-4, T-5, and T-6) according to early $SCOD_{Mn}$ concentrations. The research results are as follows. 1. As for $SCOD_{Mn}$ reduction rate, the average 29.9% in alkaline treatment groups and the average 36.9% in phosphate neutralization treatment groups were shown and so the relatively high reduction rate was shown in phosphate neutralization treatment groups. 2. After finishing the experiment, the group of the lowest $SCOD_{Mn}$ concentrations was the phosphate neutralization treatment group, T-6 with the lowest inflow concentrations. In case the final goal level of 2nd-aeration treated liquid fertilizer is assumed as concentrations less than $SCOD_{Mn}$ 3,000 ppm, it would be desired that inflow concentrations of 2nd-aeration treatment groups are adjusted less than $SCOD_{Mn}$ 5,500 ppm. 3. As for the persistence rate of nitrogen, the average 29.3% in alkaline treatment groups and the average 38.9% in phosphate neutralization treatment groups were shown and so phosphate neutralization treatment groups showed the relatively low loss rate of nitrogen, meanwhile, in the case of T-P, phosphate neutralization treatment groups maintained high concentrations (average 1,473 ppm). 4. In the event of 2nd-aeration treatment of liquid fertilizer, "alkaline fermentation treatment" condition in 'low phosphate-low nitrogen' type and "phosphate neutralization treatment" condition in 'high phosphate-high nitrogen' type are expected to be favorable.

시비처리에 따른 2년생 편백나무 용기묘의 내건성 반응특성

박진화,김재희,이도형 경상국립대학교 농업생명과학연구원 2019 농업생명과학연구 Vol.53 No.2

The main aim of this study is first to measure changes in the growth of 2-year-oldChamaecyparis obtusa containerized seedlings as influenced by fertilizer treatments and throughwater resistance measurement by water supply cut-off, second to establish a proper fertilizationplan for the promotion of drought resistance, and finally to provide basic data for healthyseeding production. For fertilization treatments, the height growth of C. obtusa seedlings was26.96±0.87 cm at a weak (1.0 g/L) fertilizer treatment, while the diameter growth of root collarwas 4.76±0.18 mm at an intermediate (2.0 g/L) fertilizer treatment (p<0.0001). Furthermore, thequality index of seedling was 0.83 at an intermediate (2.0 g/L) fertilizer treatment, which is thehighest one, while total chlorophyll content was 16.19 mg/g at a weak (1.0 g/L) fertilizertreatment. For the abscisic acid content of C. obtusa seedlings for each fertilizer treatmentduring water supply cut-off, the lowest abscisic acid content of 3671.70 ng/g was found at an intermediate (2.0 g/L) fertilizer treatment. Exceptionally, seedlings with strong (3.0 g/L) fertilizertreatment showed poor growth, caused by damaged leaves due to the excessive fertilizertreatment. Furthermore, the highest total chlorophyll content of 15.12 mg/g was observed at anintermediate (2.0 g/L) fertilizer treatment due to less effect of drought stress on chlorophyll. Wethus found the lowest abscisic acid content and the highest chlorophyll content of C. obtusaseedlings during water cutoff or drought stress at the intermediate (2.0 g/L) fertilizer treatment. The results suggested that the appropriate fertilizer treatment for improving the droughtresistance of C. obtusa seedlings is the intermediate (2.0 g/L) level. 본 연구는 편백나무 2-0 용기묘를 대상으로 시비처리에 따른 생장변화와 시비처리 후 단수처리에따른 내건성 측정을 통해 내건성 증진을 위한 적정시비 방안을 수립하여 건강한 묘목 생산을 위한 기초자료를 제공하고자 수행되었다. 시비처리에 따른 편백나무 묘목은 약도(1.0 g/L) 시비구에서 묘고생장량(26.96±0.87 cm)을 나타냈으며, 근원경 생장은 중도(2.0 g/L)의 시비구에서 가장 높은 생장량(4.76±0.18 mm)을 나타내어 다른 처리구보다 유의적으로 높게 나타났다(p<0.0001). 묘목품질지수(SQI)는 중도(2.0 g/L)의 시비구에서 0.83으로 가장 높게 나타났다. 전체 엽록소 함량은 약도(1.0 g/L)의 시비구에서 16.19 mg/g으로 가장 높게 나타났다. 단수처리에 따른 시비구별 편백나무 묘목의 ABA함량은 과시비로 인해 엽 피해가 발생하여 생육이 건전하지 못한 강도(3.0 g/L)의 시비구를 제외하면중도(2.0 g/L)의 시비구에서 가장 낮은 ABA 함량 값(3671.70 ng/g)을 나타냈고, 전체 엽록소 함량은중도(2.0 g/L)의 시비구에서 건조스트레스에 따른 엽록소의 파괴가 적어 가장 높은 엽록소 함량 값(15.12 mg/g)을 나타냈다. 따라서 편백나무는 중도(2.0 g/L)의 시비구에서 건조스트레스에 따른 ABA의 함량이 가장 적고, 엽록소의 함량은 가장 높게 나타나 편백나무의 내건성 증진을 위한 적정 시비수준은 중도(2.0 g/L)라고 판단된다.

Effects of Long-Term Fertilizer Practices on Rhizosphere Soil Autotrophic CO₂-Fixing Bacteria under Double Rice Ecosystem in Southern China

( Haiming Tang ),( Li Wen ),( Lihong Shi ),( Chao Li ),( Kaikai Cheng ),( Weiyan Li ),( Xiaoping Xiao ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.10

Soil autotrophic bacterial communities play a significant role in the soil carbon (C) cycle in paddy fields, but little is known about how rhizosphere soil microorganisms respond to different long-term (35 years) fertilization practices under double rice cropping ecosystems in southern China. Here, we investigated the variation characteristics of rhizosphere soil RubisCO gene cbbL in the double rice ecosystems of in southern China where such fertilization practices are used. For this experiment we set up the following fertilizer regime: without any fertilizer input as a control (CK), inorganic fertilizer (MF), straw returning (RF), and organic and inorganic fertilizer (OM). We found that abundances of cbbL, 16S rRNA genes and RubisCO activity in rhizosphere soil with OM, RF and MF treatments were significantly higher than that of CK treatment. The abundances of cbbL and 16S rRNA genes in rhizosphere soil with OM treatment were 5.46 and 3.64 times higher than that of CK treatment, respectively. Rhizosphere soil RubisCO activity with OM and RF treatments increased by 50.56 and 45.22%, compared to CK treatment. Shannon and Chao1 indices for rhizosphere soil cbbL libraries with RF and OM treatments increased by 44.28, 28.56, 29.60, and 23.13% compared to CK treatment. Rhizosphere soil cbbL sequences with MF, RF and OM treatments mainly belonged to Variovorax paradoxus, uncultured proteobacterium, Ralstonia pickettii, Thermononospora curvata, and Azoarcus sp.KH33C. Meanwhile, cbbL-carrying bacterial composition was obviously influenced by soil bulk density, rhizosphere soil dissolved organic C, soil organic C, and microbial biomass C contents. Fertilizer practices were the principal factor influencing rhizosphere soil cbbL-carrying bacterial communities. These results showed that rhizosphere soil autotrophic bacterial communities were significantly changed under conditions of different long-term fertilization practices Therefore, increasing rhizosphere soil autotrophic bacteria community with crop residue and organic manure practices was found to be beneficial for management of double rice ecosystems in southern China.

시비처리가 2년생 잣나무 용기묘의 생장특성에 미치는 영향

김재희,김도현,이도형 경상대학교 농업생명과학연구원 2015 농업생명과학연구 Vol.49 No.1

본 연구는 잣나무 2년생 용기묘를 대상으로 시비농도별 처리가 묘목의 형태적 생장, 물질분배, 엽록소 함량에 미치는 영향을 구명하기 위해 실시하였다. 시비처리에 따른 잣나무 용기묘는 시비 농도가 높아짐에 따라 묘고와 근원경생장이 높게 나타났으며, 1.0 g·L-1처리구에서 가장 높은 묘고 생장(3.81 cm)과 근원경생장(2.12 mm)을 나타내었다. 또한 전체 물질생산량은 1.0g·L-1처리구에서 2.58 g으로 가장 높게 나타났다. H/D율은 1.0 g·L-1처리구에서 2.70, T/R율은 0.33 g·L-1처리구에서 1.11로 가장 낮게 나타났다. 묘목품질지수는 시비농도가 높아짐에 따라 높아지는 경향을 보였고, 1.0 g·L-1처리구에서 가장 높은 0.60값을 나타내었다. 전체 엽록소함량은 시비농도가 높아짐에 따라 증가하는 경향을 보였고, 엽록소 a, b 함량 또한 높은 값을 나타내었다. 본 연구 결과 우량한 묘목생산을 위한 시비처리는 1.0 g·L-1로 조사되었으며, 양묘시 양질의 묘목을 생산하기 위한 기초자료로 활용될 것으로 판단된다. This study was carried out in order to investigate the effects of fertilizer treatment on the above ground and underground growth amount of 2 years old Pinus koraiensis seedlings. P. koraiensis container seedlings showed high seedling height and root collar diameter growth as the fertilization concentration increased, and the specimen with the fertilizer treatment of 1.0 g · L-1 showed the highest seedling height growth (3.81 cm) and root collar diameter growth (2.12 mm). Also, the whole biomass production was highest at the specimen with the fertilizer treatment of 1.0 g · L-1 as 2.58 g. H/D rate was 2.70 at the specimen with the fertilizer treatment of 1.0 g · L-1, and T/R rate was 1.11 at the specimen with the fertilizer treatment of 0.33 g · L-1. The seedling quality index tended to increase as the fertilization concentration increased, and showed the highest values as 0.60 in 1.0 g · L-1 fertilizer treatment. The total chlorophyll contents tended to increase as the fertilization concentration increased, with high contents of chlorophyll a and b. The results showed that fertilizing treatment for the superior seedling production were irradiated as 1.0 g and it is appropriate to be used as basic data for producing good seedlings and growing saplings.

양돈농가의 돈분뇨 액비화 처리 우수사례 실태조사

김수량,전상준,홍인기,김동균,이명규,Kim, Soo-Ryang,Jeon, Sang-Joon,Hong, In-Gi,Kim, Dong-Kyun,Lee, Myung-Gyu 한국축산환경학회 2012 축산시설환경학회지 Vol.18 No.2

본 연구에서 조사된 일부 선도적인 양돈농가에서는 설치 운영하는 호기적 액상발효 공정은 대부분의 농가가 공정별 설계인자 및 물리 화학적인 성분변화의 특성보다는 경험적인 운영을 토대로 액비를 생산하고 있는 것을 알 수 있었다. 김 등의 조사연구에 따르면 가축분뇨의 발생단계인 분뇨수거방식은 현재 국내 돈사의 70% 이상이 슬러리 형태로 분뇨가 배출되고 있는 것으로 보고되고 있다. 양돈분뇨의 수거방식과 배출형태가 대부분 슬러리라는 사실은 양돈분뇨의 적정처리에 의한 환경오염 차단과 자원화 이용과정에 적용될 각종 공법 및 시스템 적용이 돈사 슬러리부터 집중 관리해야 함을 시사한다. 이처럼 가축분뇨 액비화의 적정처리에 있어서는 발생단계 뿐만 아니라 액비가 토양에 환원되기 직전까지의 모든 과정이 전반적으로 고려되어야 한다. 본 연구의 조사대상 농가의 가축분뇨 액비화 운영에 있어서 핵심적으로 구분 할 수 있는 단계를 주요사항 별로 구분하여 Table 8에 나타내었다. 가축분뇨 액비화 처리에 있어서 고액분리 단계의 경우 고액분리 방식과 효율에 따라 BOD (생물학적산소요구량), COD (화학적산소요구량), VFA (휘발성지방산) 등의 제거율의 차이가 있는 것으로 보고되고 있으며, 이는 후단공정 즉 주발효조에서의 공기주입량의 산정, 공기공급방식, 발효기간 및 2차 발효 등 운영방식 결정에 주요한 요인이 될 수 있다. 이와 같이 액비가 생산되어 토양에 환원되기까지의 과정은 각각의 주요한 요소들이 유기적으로 연계되어 있으며, 농가 현장을 방문조사하고, 생산되는 액비를 비교 분석한 결과 액비화 공정에 있어서 필수적인 핵심적 단계를 도출 할 수 있었다. 이에 대한 주요내용은 다음과 같다. 1. 돈사 슬러리 관리 단계 돈사 슬러리는 혐기부패를 최대한 방지하며, 주요 악취물질인 $H_2S$, $NH_4$, VFAs의 생성을 억제하여야 한다. 돈사환경을 개선하기 위한 방법으로서는 부숙이 완전히 완료되어 유용미생물이 활성화된 액비를 돈사 세척수로 이용하거나, 슬러리 피트에 일부 투입하여 단순 저장일수를 처리일수로의 개념적 전환이 필요하다. 2. 고액분리 단계 일반적으로 BOD의 주요원인인 고농도의 유기물질은 대부분 분뇨의 고형성분에 포함되어 있기 때문에 물리적 처리공정인 고액분리 단계에서 최대한으로 제거하여 후단공정인 주발효조의 부하를 최소화 시킨다. 3. 발효처리-공기공급 단계 축산농가 마다 사료의 종류나 관리방법 등이 각기 다르므로 배출되는 슬러리의 성상을 파악하여 물질수지와 처리일수에 기초한 발효조 용량과 공기주입량의 산정이 필요하다. 또한 공기확산 및 막힘현상을 고려한 자동제어 장치나 발효조의 상태를 감시 제어하기 위한 인자로서 pH 및 산화환원전위 등을 실시간으로 모니터링 하는 것이 중요하다. 4. 발효처리-미생물, 반송 단계 일반점검 (온도, pH, 용존산소, 산화환원전위, 전기전도도 등)과 정밀점검 (BOD, COD, VFAs, SS, N, P 등)의 주기적인 점검을 통하여 미생물 생장에 알맞은 유입농도와 반송량을 결정하고 농가환경에 맞는 발효조 운전방식을 확립 할 필요가 있다. 5. 후숙처리-최종액비 단계 후숙조에서는 공기과잉 투입으로 인한 질소성분의 손실을 방지하고, 유용미생물을 이용하여 토양환원에 적합한 액비를 제조한다. 가스발생에 의한 작물생육 피해를 방지할 수 있는 액비 안정화 운전기술을 확립하고, 살포시기 The purpose of the study is to collect basis data for to establish standard administrative processes of liquid fertilizer treatment. From this survey we could make out the key point of each step through a case of effective liquid manure treatment system in pig house. It is divided into six step; 1. piggery slurry management step, 2. Solid-liquid separation step, 3. liquid fertilizer treatment (aeration) step, 4. liquid fertilizer treatment (microorganism, recirculation and internal return) step, 5. liquid fertilizer treatment (completion) step, 6. land application step. From now on, standardization process of liquid manure treatment technologies need to be develop based on the six steps process.

PTEN mutation predicts unfavorable fertility preserving treatment outcome in the young patients with endometrioid endometrial cancer and atypical hyperplasia

Yu Xue,Youting Dong,Yaochen Lou,Qiaoying Lv,Weiwei Shan,Chao Wang,Xiaojun Chen 대한부인종양학회 2023 Journal of Gynecologic Oncology Vol.34 No.4

Objective: This study aimed to investigate the impact of molecular classification and PTEN, KRAS and PIK3CA gene mutation on the outcome of fertility-preserving treatment in the patients with endometrioid endometrial cancer (EEC) and endometrial atypical hyperplasia (EAH). Methods: This is a single-center retrospective study. A total of 135 patients with EEC and EAH receiving fertility-preserving treatment and molecular classification were reviewed. The distribution of the four types of molecular classification was described. The impact of non-specific molecular profile (NSMP), mismatch repair-deficiency (MMRd), and PTEN, KRAS and PIK3CA gene mutation on the outcome of fertility-preserving treatment was analyzed. Results: Of the patients analyzed, 86.7% (117/136) were classified as having NSMP; 14 (10.4%), MMRd; 1 (0.7%), POLEmut EAH; and 3 (2.2%), p53abn EEC. The patients having NSMP and MMRd achieved similar 16-, 32-, and 48-week complete response rates. The patients harboring tier I and tier II PTEN mutations (PTENmut-Clin) achieved lower cumulative 32-week CR rates than those with PTEN-others (without PTENmut-Clin) (22/47, 46.8% vs. 50/74, 67.6%; p=0.023; odds ratio=0.422; 95% confidence interval [CI]=0.199–0.896). Insulin-resistance (hazard ratio [HR]=0.435; 95% CI=0.269–0.702; p=0.001) and PTENmut-Clin (HR=0.535; 95% CI=0.324–0.885; p=0.015) were independent negative predictors for lower 32-week CR rates. Conclusion: PTENmut-Clin is an independent risk factor for unfavorable fertility-preserving treatment outcomes in the patients with EEC and EAH. The patients with MMRd receiving fertility-preserving treatment achieved outcomes similar to those of the patients with NSMP. The molecular profiles might guide fertility-preserving treatment in the prognosis and clinical decisions.

시비수준별 소나무 용기묘의 생장 특성 및 가시적 피해

차영근,최규성,송기선,구다은,이하나,성환인,김종진 (사) 한국생물환경조절학회 2019 생물환경조절학회지 Vol.28 No.1

The present study investigated pine trees, which forms a major plantation species in Korea, with the objective of improving the survival rate of pine trees after planting. Growth responses and characteristics were assessed by controlling the level of fertilizer application, which is a basic controlling the growth of pine seedlings, to identify the optimal fertilization treatment. Pine tree seedlings were grown in 104 containers and were examined 8 weeks after planting. Stem height and were measured at 4-week intervals. In terms of fertilization treatment for 1-0 pine seedlings, the treatment group with gradually-increasing fertilizer concentration (500→1000→1000 →1000mg·L-1) had the biggest increase in stem height and diameter at the root. The survey results indicated that the increased concentration treatment group and the gradually-increasing concentration treatment group had more growth compared with that in the fixed concentration treatment group. The gradually-increasing concentration treatment group (500→1000→1000→1000mg·L-1) had the highest total dry matter production. Nine weeks after fertilization, the tips of the pine leaves turned yellow in the fixed concentration treatment group (3000mg·L-1). The same phenomenon was observed in the treatment group in which the concentration was increased to 2000mg·L-1, and in the gradually- increasing concentration treatment group, when the concentration was raised up to 2000mg·L-1. We concluded that the optimal fertilization conditions for producing healthy pine 1-0 seedlings involve fertilizing once a week with Multifeed 19 at 500mg·L-1 during the seedling period, Multifeed 19 at 1000mg·L-1 during the rapid growth period, and Multifeed 32 at 1000mg·L-1 during the maturation period. 본 연구는 우리나라의 대표적인 조림수종인 소나무를대상으로 조림지 식재 후 활착률을 높이기 위하여 상대적으로 생육이 좋은 용기묘(1-0)로 생산하고자 할 때, 기본적인 생육환경 요인들 중 시비처리 수준에 따른 생장 반응특성을 조사하고 이를 통하여 적정 시비 수준을 구명하고자 실시하였다. 104구 용기에서 생육된 소나무 용기묘는파종 후 약 8주 후부터, 4주 간격으로 간장 및 근원경을측정하였다. 그 결과, 소나무 1-0 용기묘의 시비처리는변형증가농도처리군의 500→1000→1000→1000mg·L-1에서간장, 근원경 생장이 좋았으며, 전체적으로 고정농도처리군보다 증가농도처리군과 변형증가농도처리군이 더 좋은생장을 한 것으로 나타났다. 총건물생산량은 변형증가농도처리군의 500→1000→1000→1000mg·L-1 처리구가 가장 높았다. 시비처리 후 9주째부터 고정농도처리구3000mg·L-1 시비처리에서 소나무잎의 끝부분이 노랗게변하는 증상이 관찰되었다. 이와 같은 증상은 증가농도처리구의 2000mg·L-1까지 농도를 증가시킨 처리구와 변형증가농도처리군의 2000mg·L-1까지 농도를 증가 시킨처리구에서 발견되었다. 본 연구를 통하여 소나무 1-0 용기묘 생산을 위한 시비처리는 시비는 유묘형성기에Multifeed 19로 500mg·L-1, 빠른생장기에 Multifeed 19로1000mg·L-1, 경화기에 Multifeed 32로 1000mg·L-1를 일주일에 한번씩 지속적으로 실시하는 것이 우량한 용기묘를 생산하는데 바람직한 것으로 판단된다.

고형돈분 처리에 따른 벚나무의 유묘생장 및 질소,인산 흡수에 관한 연구

염창호 ( Chang Ho Yeum ),임유미 ( You Mi Lim ),채승민 ( Seung Min Chae ),이창헌 ( Chang Heon Lee ) 한국환경복원기술학회(구 한국환경복원녹화기술학회) 2011 한국환경복원기술학회지 Vol.14 No.6

Prunus serrulata var. serrulata f. spontanea (E. H. Wilson) C. S. Chang was treated with dried swine excrement at various concentration levels, and their growth reactions and the contents of total kjeldahl nitrogen and total phosphoric acid were analyzed. The result is as follows; 1. When the plants were treated with 0.25% of dried swine excrement, the seed germination rate of Prunus serrulata var. serrulata f. spontanea was higher than that of the control. However, it showed a tendency to decrease when the treatment concentration got higher than 0.25%. 2. The growth rate of species was highest when they were treated with 0.25% of dried swine excrement. It tended to decrease with higher treatment concentration than 0.25%. Significant difference was clear between the control and the experimental plants of Prunus serrulata var. serrulata f. spontanea. 3. The contents of nitrogen and phosphoric acid in Prunus serrulata var. serrulata f. spontanea also got higher as the concentration of dried swine excrement increased. However, the content of total kjeldahl nitrogen was highest in the leaves, and followed by the roots and stems. In contrast, the content of total phosphoric acid were highest in the roots, and followed by the leaves and stems. Therefore, stems had the lowest contents of both nitrogen and phosphoric acid. 4. The contents of total kjeldahl nitrogen and total phosphoric acid in the soil were measured before and after the growth experiment of Prunus serrulata var. serrulata f. spontanea treated with dried swine excrement. The contents of nitrogen and phosphoric acid significantly decreased after the experiment. In conclusion, the contents of total kjeldahl nitrogen and total phosphoric acid accumulated in the plants increased as the concentration level of dried swine excrement got higher. The seed germination and plant growth rates were best at 0.25% treatment of dried swine excrement. The treatment of dried swine excrement may bring high effects on increasing the plant growth rate but could damage the plants with higher concentration than they need. Different optimal concentration levels of dried swine excrement for different plants should be found before it is used as fertilizer.