황련(黃連)의 Helicobacter pylori에 대한 항균 효과

서운교,신정인,Seo Un-Kyo,Shin Jeong-In 대한한방내과학회 2003 大韓韓方內科學會誌 Vol.24 No.2

Water and ethanol extracts of 67 species of medicinal plants were tested to determine antimicrobial activity against Helicobacter pylori. Among them, the extracts of Coptis japonica showed the best antibacterial activity. The extract of C. japonica showed four major spots on TLC plate and the Rf values of the spots were 0.07, 0.13, 0.21 and 0.73, respectively. Except for the spot of Rf 0.73, other three spots inhibited the cell growth of H. pylori. As shown in HPLC analysis, three antimicrobial spots contain berberine, major antimicrobial substance of C. japonica. However, the spot of Rf 0.13 had higher activity than berberine. The concentrated water extract of three prescribed medicines related with C. japonica showed good antibacterial activity against H. pylori.

황련 추출물을 함유한 화장품의 민감성 피부 안전성 연구

민경남 한국응용과학기술학회 2023 한국응용과학기술학회지 Vol.40 No.3

This study was to verify the skin safety of Coptis Japonica extract(CJE) -containing cosmetics by patching them on sensitive skin and checking its stimulating level. 32 subjects diagnosed with sensitive skin by the result of the lactic acid injury test, were subjected to 20 ㎕ of solubilized essence mixed with 2% CJE on the test subjects’ back and closed patched for 24 hours. No stimulus reactions were found by bare eyes and scored 0 stimulus figure points after they were taken off in 30 minutes, 24 hours, and 48 hours. Coptis japonica’s medical efficacies such as antioxidant, and anti-inflammatory are expectable to be used as functional cosmetics, and this research would be the foundation of further research into its cosmetical usage.

黃連과 구성 생화합물의 약리작용에 대한 고찰

김기배(Ki Bae Kim),이형탁(Hyung Tak Lee),구경회(Kyung Howi Ku),홍진우(Jin Woo Hong),조수인(Su In Cho) 대한한의학회 2012 대한한의학회지 Vol.33 No.3

TD GC/MS를 이용한 황벽, 황련 및 애기똥풀의 향기 성분 분석

정진순,Jung, Jin Soun 한국섬유공학회 2013 한국섬유공학회지 Vol.50 No.5

Volatile compounds in Phellodendron amurense Ruprecht, Coptis japonica Makino, and Chelidonium majus var. asiaticum were analyzed by thermal desorption gas chromatography/mass spectrometry (TD GC/MS). Thirty-eight compounds-20 hydrocarbons, 2 alcohols, 9 aldehydes, 2 ketones, 3 ethers, a carboxylic acid, and a heterocyclic acid-were identified in Phellodendron amurense Ruprecht. Twenty-five compounds-18 hydrocarbons, an alcohol, an aldehyde, 3 ketones, an ester, and a carboxylic acid-were identified in Coptis japonica Makino. Twenty-four compounds-14 hydrocarbons, an alcohol, 3 aldehydes, 2 ketones, an ether, an ester, a carboxylic acid, and a heterocyclic acid-were identified in Chelidonium majus var. asiaticum. Monoterpene hydrocarbon compounds were the major compounds found in Phellodendron amurense Ruprecht (64.16%), Coptis japonica Makino (95.02%), and Chelidonium majus var. asiaticum (92.05%). Unlike Coptis japonica Makino and Chelidonium majus var. asiaticum, Phellodendron amurense Ruprecht was found to contain large amounts of aldehyde compounds. The nitrogen compound, N-vinyl-2-oxazolidone, was the most common compound found in Phellodendron amurense Ruprecht and Coptis japonica Makino. Trans-Caryophyllene of sesquiterpene was the most common compound found in Phellodendron amurense Ruprecht, Coptis japonica Makino, and Chelidonium majus var. asiaticum.

Differentiating Coptis chinensis from Coptis japonica and other Coptis species used in Coptidis Rhizoma based on partial trnL-F intergenic spacer sequences

도의정,오승은,이미영,고병섭 한국유전학회 2014 Genes & Genomics Vol.36 No.3

Dried rhizomes of Coptis species are utilized as‘‘Coptidis Rhizoma’’ (CR), an important herbal medicinalmaterial in traditional Chinese medicine. Almost all CRstraded in the Korean herbal medicine market originate fromCoptis chinensis (‘‘Chun Hwang-Lyun’’ in Korean medicalterminology). Other minor CRs originate from Coptisjaponica (‘‘Il Hwang-Lyun’’). Although there is an obviousdiscrepancy in the price of traded CRs in the herbal marketdepending on the Coptis species, CRs originating from C. chinensis and C. japonica are often confused. Furthermore,the CR traded as ‘‘Chun Hwang-Lyun’’ is occasionallymixed with rhizomes of Coptis deltoidea and/or Coptisomeiensis. Therefore, we sought to discriminate C. chinensisfrom C. japonica, as well as C. deltoidea and C. omeiensis, by using nucleotide sequence differences in thepartial trnL-F intergenic spacer. We developed an efficientreal-time polymerase chain reaction (PCR)-based discriminationassay to separate samples of C. chinensis fromthose of C. japonica without the need to separate the DNAmarkers by using gel electrophoresis. In addition, wedeveloped a multiplex PCR method with which we wereable to discriminate samples of C. chinensis from those ofC. deltoidea and C. omeiensis by amplifying the 153-bpDNA marker in C. chinensis in a single PCR process.

황련 추출물을 함유한 에센스의 피부장벽강화 및 보습 효과

민경남 한국응용과학기술학회 2023 한국응용과학기술학회지 Vol.40 No.5

The purpose of this study is to verify the effect of essence containing Coptis Japonica extract on skin barrier strengthening and moisturizing. The essence containing the Coptis Japonica extract was used by 22 subjects for four weeks, and the amount of percutaneous water loss (g/m²h) and water (A.U.) in the designated upper limit were measured. As a result of measuring percutaneous water loss using a vapometer, it was found to be 11.08±2.55 after 4 weeks of use from 14.82±4.01 before use, and the significance probability decreased significantly by 25.27% to p<0.001. The amount of skin moisture measured by the Corneometer was found to be 46.73±7.45 after use from 31.73±8.09 before use, and the significance probability increased by 47.26% to p<0.001, and the subject's subjective efficacy evaluation through a questionnaire showed that all subjects were 100% satisfied without skin adverse reactions. As a result of this, it has been confirmed that Coptis Japonica essence is effective in strengthening skin barriers and moisturizing, which is expected to be a cornerstone for the development of cosmetics using various activities of Coptis Japonica extract in the future.

정향과 황련 혼합물의 항균 및 항염증 활성 연구

이은홍,정은미,권현지,이지혜,우봉현,박성민,박진한,정지욱 한국응용과학기술학회 2023 한국응용과학기술학회지 Vol.40 No.6

본 연구는 정향, 황련 추출물을 혼합하여 Malassezia furfur에 대한 항균 능력을 확인하고 최적의 혼합물을 만들어 항산화 및 항염증 능력을 평가하기 위해 수행되었다. 정향과 황련을 70% 에탄올, 100% 메탄올, 열수로 추출하여 항균 능력을 평가하여 정향은 100% 메탄올 추출물, 황련은 열수 추출물이 가장 항균 능력이 높은 것을 확인하였다. 또한 두 추출물을 혼합하여 비율별로 항균 능력을 평가하였을 때 9:1 비율이 가장 우수한 활성을 보였고 혼합물의 항산화 활성이 뛰어남을 확인하였다. Raw 264.7 세포에서 생존율에 영향을 미치지 않는 1, 10, 50, 100 µg/mL에서 LPS를 이용하여 염증 반응을유도하고 항염증 활성을 확인한 결과 10, 50, 100 µg/mL에서 No 생성 저해율과 IL-6 발현 저해율 및COX2, iNOS 단백질 발현 저해 활성이 뛰어남을 확인하였다. 본 연구를 통해 정향 메탄올 추출물 및 황련 열수 추출물의 혼합물은 항균, 항염증 효능이 뛰어나기에 기능성 화장품의 천연 소재로써 사용이가능할 것으로 사료된다. This study was conducted to check the anti-bacterial ability against Malassezia furfur by mixing Szygium aromaticum and Coptis japonica extracts and to evaluate the antioxidant and anti-inflammatory ability by creating an optimal mixture. Szygium aromaticum and Coptis japonica were extracted with 70% ethanol, 100% methanol, and water to evaluate the antibacterial ability, and it was confirmed that 100% methanol extract of Szygium aromaticum and Coptis japonica water extract had the highest anti-bacterial ability. In addition, when the two extracts were mixed and the anti-bacterial ability was evaluated by ratio, the ratio of 9:1 showed the best activity, and it was confirmed that the antioxidant activity of the mixture was excellent. In Raw 264.7 cells, LPS was used to induce inflammatory responses and confirmed anti-inflammatory activity at 1, 10, 50, and 100 μ g/mL that did not affect survival, and it was confirmed that NO-production inhibition and IL-6 expression inhibition and COX2 and iNOS protein expression inhibition activity were excellent at 10, 50, and 100 μg/mL. Through this study, it is thought that the mixture of Szygium aromaticum 100% methanol extract and Coptis japonica water extract can be used as a natural ing

肺炎 誘發菌의 生育을 抑制하는 韓藥材 探索에 關한 硏究

정병운,서운교,정지천,한영환 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

韓藥材 중 肺炎의 치료효과가 기대되는 淸熱化痰, 止咳平喘藥을 중심으로 23種의 藥材를 사용하여 肺炎을 유발하는 K. pneumoniae, S. pyogenes 및 S. pneumoniae 세균의 생육을 억제하는 藥材를 탐색하고, 탐색된 藥材의 추출물에 대한 세균의 最小生育抑制濃度를 측정하였다. 韓藥材의 水溶性 추출물 중 黃連, 胡黃蓮, 敗醬, 黃芩이 K. pneumoniae에서, 黃連이 S. pyogenes에 대해서 생육억제 효과가 있었으며, S. pneumoniae에 대한 比較沮止環의 直徑은 黃連, 鳥梅, 五味子, 黃芩이 우수하였으나 黃連 추출물 사용시 병원균 모두에서 生育沮止環의 크기가 가장 크게 나타났으며 對照群로 사용된 大腸菌과 枯草菌에서도 어느 정도의 抗細菌 효과를 볼 수 있었다. 에탄올 추출물에서는 敗醬, 黃芩, 五味子, 鳥梅가 K. pneumoniae 세균의 생육을 억제하였으며, S. pyogenes 세균은 黃連, 五味子, 鳥梅, 馬兜鈴찾, 黃芩에 의하여 생육이 억제되어 黃連은 水溶性 및 에탄올 溶解性 주출물에서 모두 우수한 抗細菌 효과를 보여주고 있다. 각 세균에 대한 最小生育沮止濃度(MIC)는 K. pneumoniae 세균일 경우 黃連 및 黃芩의 水溶性 추출물과 鳥梅 및 敗醬의 에탄올 溶解性 추출물이 유의성을 보였으며, S. pyogenes 세균은 黃連의 水溶性 및 에탄올 溶解性 추출물과 鳥梅의 에탄올 溶解性 추출물, S. pneumoniae 세균은 黃連과 鳥梅의 水溶性 및 에탄올 溶解性 주출물에서 유의성이 나타났다. 이상의 결과를 통하여 黃連, 五味子, 鳥梅, 黃芩, 胡黃蓮, 敗醬의 水溶性 및 에탄올 溶解性 추출물이 肺炎 誘發菌f K. pneumoniae, S. pyogenes, S. pneumoniae에 對하여 우수한 生育抑制 作用을 나타냄을 알 수 있었다. The various oriental herbal medicines, which have usually been used for treatment of reducing fever, purging intense heat and detoxication, were screened to determine the antibacterial activity and the minimal inhibitory concentration against pulmonary disease-causing Klebsiella pneumoniae, Streptococcus pyogenes, and Streptococcus pneumoniae. The results obtained were as follows: 1. Among the 23 oriental medicines tested, the water-soluble extracts of Coptis japonica, Scutellaria baicalensis and Picrorrhiza kurroca showed the antibacterial activity against K. pneumoniae and that of C. japonica against S. pyogenes. The antibacterial activities of C. japonica, Prunus mume, Schizandra chinesis, Scutellaria baicalensis were also found against S. pyogenes. When C. japonica was used, the high antibacterial activity was shown against Bacillus subtilis and other extracts showed a little activity against B. subtilis and E. coli as a control. 2. The ethanol-soluble extracts of Patrinia scabriosaefolia, P. mume, S. baicalensis, S. chinensis showed the antibacterial activity against K. pneumoniae and those of S. baicalensis, C. japonica, S. chinensis P. mume against S. pyogenes and S. pneumoniae. However, those extract showed a little antibacterial activity against B. subttilis and E. coli except for that the extract of C. japonica showed comparatively high growth inhibition of B. subtilis. 3. Among the medicinal herbs tested, the water- and ethanol extracts of C. japonica showed very extcellent antibacterial activity against the pathogenic bacteria and controls. 4. When the water-soluble extracts of C. japonica and S. baicalensis, minimal inhibitory concentrations (MICs) against K. pneumoniae were 10 ㎎/㎖ and 22 ㎎/㎖, respectively. The MICs of the ethanol-soluble extracts of P. mume and P. scabriosaefolia were 5 ㎎/㎖ and 20 ㎎/㎖, respectively. 5. For the MICs against S. pyogenes, C. japonica showed 15 ㎎/㎖ with the water-soluble extract and P. mume and C. japonica with the ethanol-souble extract did 5 ㎎/㎖ and 10 ㎎/㎖, respecitively. 6. For the MICs against S. pneumoniae, C. japonica and P. mume with the water- and ethanol-souble extract showed 5 ㎎/㎖ and 10 ㎎/㎖, respecitively. As a result, the highest antibacterial activity was found in the water- and ethanol-soluble extracts of C. Japonica against pulmonary disease-causing bacteria, K. pneumoniae, S. pyogenes, and S. pneumoniae. Also, the water- and ethanol-soluble extracts of S, chinensis, P. mume, S. baicalensis and P. kurrooa showed high antibacterial activities.

Isoquinoline alkaloids from Coptis japonica stimulate the myoblast differentiation via p38 MAP-kinase and Akt signaling pathway

Lee, H.,Tuong, L.T.,Jeong, J.H.,Lee, S.J.,Bae, G.U.,Ryu, J.H. Pergamon Press 2017 Bioorganic & medicinal chemistry letters Vol.27 No.6

To overcome the muscle atrophy, such as cachexia and sarcopenia, we tried to find myogenic agents from medicinal plants. From myogenic extract of Coptis japonica, we purified six isoquinoline alkaloids and evaluated their effects on transactivation of myoD and MHC expression in C2C12 cells during differentiation process. Among obtained compounds, magnoflorine most efficiently enhanced the myoblast differentiation by activating the p38 MAP kinase and Akt pathway, and also increased the number of multinucleated and cylinder-shaped myotubes. These results propose that magnoflorine from Coptis japonica might be a promising lead compound for the development of anti-muscle atrophy drug.

황련 열수추출물을 처치한 인간 대장암 세포 SNU-81에서의 단백질 발현 변화

유태모,김병수,유병철,유화승,Yoo, Tae-Mo,Kim, Byung-Soo,Yoo, Byong-Chul,Yoo, Hwa-Seung 대한약침학회 2009 Journal of pharmacopuncture Vol.12 No.1

Background : Anticancer effects of herbal medicine have been reported in various types of cancer, but the systematic approaches to explain molecular mechanism(s) are not established yet. Objective : To find the anticancer-effect and mechanism(s) of Water Extract of Coptis japonica (WECJ) colon cancer cell (SNU-81). Methods : We first selected 11 herbals, and anti-cancer effects of water-extracts from those herbals have been tested in human colon cancer cell line, SNU-81. Among the tested herbals, the WECJ significantly reduced proliferation of SNU-81. To establish a basis of understanding for anti-cancer mechanism, whole proteins have been obtained from SNU-81 harvested at 48 and 96 hrs after the treatment of WECJ, protein expression has been profiled by 2DE-based proteomic approach. Results : Various changes of the protein expression have been monitored, and most frequent dysregulation was found in the molecular chaperons including heat shock protein 90-alpha (Hsp90-alpha), 14-3-3 protein epsilon, T-complex protein 1 subunit alpha, protein disulfide-isomerase A3, and calreticulin. Interestingly, proliferation-associated protein 2G4 has been up-regulated, and it suggests the possible effect of Coptis japonica on ErbB3-regulated signal transduction pathway and growth control of human colon cancer cells. Conclusion : Based upon the present findings, the further study will focus on monitoring various cancer survival factors after artificial regulation of the proteins identified, and it would be the basis for the understanding of the Coptis japonica anti-cancer effect(s) at the molecular level.