Melanoma B-16에서 $IL-1{\beta}$ 유발 GnT-III, V 활성에 대한 과루인(瓜蔞仁), 황금(黃芩) 추출물의 영향(影響)

서운교,정지천,Seo, Woon-Gyo,Jeong, Ji-Cheon 대한암한의학회 1999 大韓癌韓醫學會誌 Vol.5 No.1

The activity of N-acetylglucosamitnyltransferase(GnT) III and V on a Melanoma B-16 was examined after incubation with interleukin 1 (IL-1). While augumenting cell proliferation, IL-1 resulted in a decrease of GnT-III activity and an increase of GnT-V activities. Consistant with this, Melanoma B-16 cultured with IL-1 showed increased affinlity to Daturam stramonium lectin, which recognizes asialo-tri- and asialeo-tetra-antenery N-linked oligosaccharides. These results indicate that IL-1 modulate glycosyltransferase activity and the oligosaccharide structure of target cells. On the other hand, to investigate whether or not TKM-SG affect GnT-V gene expression in lung metastatic carcinoma, we used RT-PCR methods. TKM-SG treated cell lines showed low levels of secretion of GnT-V mRNA transcription as elucidated by RT-PCR. Thus, with together lower GnT-V activity levels in the medium, TKM-SG was highly effective for lung cancer metastasis treatment and it was concluded that the medicine can be used as a potent anti-lung cancer metastasis medicine.

황련(黃連)의 Helicobacter pylori에 대한 항균 효과

서운교,신정인,Seo Un-Kyo,Shin Jeong-In 대한한방내과학회 2003 大韓韓方內科學會誌 Vol.24 No.2

Water and ethanol extracts of 67 species of medicinal plants were tested to determine antimicrobial activity against Helicobacter pylori. Among them, the extracts of Coptis japonica showed the best antibacterial activity. The extract of C. japonica showed four major spots on TLC plate and the Rf values of the spots were 0.07, 0.13, 0.21 and 0.73, respectively. Except for the spot of Rf 0.73, other three spots inhibited the cell growth of H. pylori. As shown in HPLC analysis, three antimicrobial spots contain berberine, major antimicrobial substance of C. japonica. However, the spot of Rf 0.13 had higher activity than berberine. The concentrated water extract of three prescribed medicines related with C. japonica showed good antibacterial activity against H. pylori.

노인(老人) 천증(喘證)에 대(對)한 임상적(臨床的) 고찰(考察)

서운교,정지천,이원철,Seo, Un-Kyo,Jeong, Ji-Cheon,Lee, Won-Chul 대한한방내과학회 1993 大韓韓方內科學會誌 Vol.14 No.1

Clinical observation was done on 372 Cheon-Jeung patients more than 65 years of age in Department of Internal Medicine, Oriental Medical Hospital in Dong Guk Univ. from December 1990 to November 1991. The results were as follows; 1. The ratio of male to female was 1.2:1. The age distribution showed the large number in the 65-69 year group, the female more than the male in 8th decade. 2. The peak age in the on set was 7th decade(44.4%). 3. The ratio of the season distribution was as follows, everytime 62.6%, fall and winter 25.5%. 4. Duration from on set, the most frequence was $5{\sim}10$ years(29.6%). 5. Chief complaints were represented cough, laryngeal stridorand, dyspnea. 6. In past illness, Pulmonary. The was most predominent(29.7%) followed by allergic disease(8.1%), bronchitis(7.2%) in turn. 7. In complication, Pul. Tbc was most predominent(21.1%) followed by ronchiectasis (12.7), cardiac disease(11.3%). 8. Predisposing factors in the Geriatric athma were represented chiefly exercising(41.8%), the others is Pung-Han-Neng(27.6%), respiratory infection(12.7%) etc. 9. The recipe which used in treatment were Gamigeumsuyukgun-jeon, Gamimahwangyunpai-tang, Gamiyunpai-tang, Sojaganggi-tang, Cheongsangboha-tang etc. 10. Duration of treatment was noted within 10 days was 39.2%, $11{\sim}30$ days was 40.6%, over 60 days was 7.3%. 11. About 77.3% patients were improved.

노인(老人) 뇌졸중(腦卒中)에 대(對)한 임상적(臨床的) 고찰(考察)

서운교,정지천,이원철,Seo, Un-Kyo,Jeong, Ji-Cheon,Lee, Won-Chul 대한한방내과학회 1993 大韓韓方內科學會誌 Vol.14 No.2

Clinical observation was done on 92 cases of Occlusive CVD, Cerebral hemorrhage (Subarachnoid hemorrhage) which were confirmed by Brain CT scan and observed for over 4 weeks, among the 121 cases which were more than 65 years of age. they admitted to the Dept. of Internal Medicine, Oriental Medical Hospital in Dong Guk Univ. from July 1992 to June 1993. The result were as follows; 1. In this study, Occlusive CVD was 74 cases, Cerebral hemorrhage (Subarachnoid hemorrhage was 2 cases) was 18 cases. 2. The ratio of male to female was 1.09:1. The age distribution showed the large number in the 65-69 year group(52.2%), 7th decade was 44.5% over 8th decade was 3.3% in ratio. 3. The site of Occlusive CVD was most common at MCA. the site of Cb-hemorrhage was most common at Basal ganglia. 4. The most common preceding disease was hypertension(47.8%) and the next were diabetes mellitus(14.1%), heart desease(14.1%). 5. Recurrence rate was 33.7% and 2nd attack was 20.7%, 3rd attack was 8.7%, 4th attack was 4.3% 6. Predisposing factors in Occlusive CVD were initiated usually during resting and sleeping, and that in Cb-hemorrhage were represented chiefly exercising(66.6%). 7. The smoker was 52.2%, the drinker was 32.6% in whole group. the drinker was 61.1% by the Cb-hemorrhage. 8. The ratio of the season distribution was as follow, fall 35.8%, winter 29.3%, spring 19.6%, summer 15.3%, that of the month distribution was november 15.2%. 9. Duration from on set in Occlusive CVD, 60.8% was within 5 days, that in Cb-hemorrhage, 77.8% was within 5 days. 10. Level of consciousness on attack was clear 42.2%, lethargy and mental change(dull, stupid etc.) 41.3%. The common symptoms were motor disturbance(90.2%), verbal disturbance(65.2%), headache(43.5%). 11. The physical theraphy of Occlusive CVD has been performed 75.7% in whole group and the average beginning time was 6.4 days, and that of Cb-hemorrhage has been performed 61.1% in whole group and the average beginning time was 13 days. 12. Duration of hospitalization was noted 11-20 days was 31.5%, over 21 days was 46.8%, and the average admission was 22.7 day(Occlusive CVD), 32days (Cb-hemorrhage). 13. The main complication were observed in the studies; urinary tract infection and pneumonia were noted in 6.5%, bed sore in 5.4%. 14. The ratio of systolic blood pressures in admission and discharge decreased from 58.7% to 28.3% in over 160 mmHg, that of diastolic blood pressures in admission and discharge decreased from 72.8% to 51.1% in over 90 mmHg. In 31(33.7%) of the 92 cases it showed the glucose levels of more than normal. 15. The patients have done family history of hypertension and C.V.A were 32.6% of all 16. Occlusive CVD In 83.8% and Cb-hernorrhage in 72.2% were improved 17. The herb medications were various Sunghyanggeonggisan, Sopungtang, CHunmagudeungeum were used most frequently and Gamidaebotang, Boyangwhanotang, Gagamyunjotang, Mangeumtang etc. were used as discharge.

Melanoma B-16 에서 IL-1β 유발 GnT-Ⅲ,ⅴ 활성에 대한 瓜蔞仁 , 黃芩 추출물의 影響

徐雲敎,鄭智天 대한한방종양학회 1999 대한한방종양학회지 Vol.5 No.1

The activity of N-acetylglucosamitnyltransferase(GnT) Ⅲ and V on a Melanoma B-16 was examined after incubation with interleukin 1 (IL-l ). While augumatting cell proliferation, IL-1 resulted in a decrease of GnT-Ⅲ activity and an increase of GnT-V activities. Consist-u with this, Mdamnu B-16 cultured with IL-l showcd increased affinlity to Daturam stramonium latin, which raogfuzes asialo-tri- and asialeo-tetra-antenery N-linked oligosaccharides. These results indicate that IL-1 modulate glycosyloaderse activity and the oligosaccharide sVtuture of target cells. On the omer hand, to investigate whether or not TKM-SG affect GnT-V gene expression in lung metastatic carcinoma, we used RT-PCR methods. TKM-SG treated cell lines showed low leve1s of secretion of GnT-V mRNA transcription·as elucidated by RT-PCR. Thus, with together lower GnT-V acnuty levels in the medium, TKM-SG was highly enative for lung cancer metastasis treatment and it was concluded that the medicine can be used as a potent anti-lung cancer metastasis medidcine.

老人 喘證에 對한 文獻的 考察

徐雲敎,鄭智天 대한한방성인병학회 1995 韓方成人病學會誌 Vol.1 No.1

Study on Geriatric asthma based on literature and thesis, etc. Knowing the cause of the oriental medicine and treatment. The results obtained are as follows; 1. The cause was divided into asthenia of the kidney, the lung, the spleen and stomach. 2. Proximate cause was divided into exogenous pathogenic factor, food, emotion, overwork and chronic case. Oriental pathology was deficiency in origin and excess in superficiality, excess in the upper and deficiency in the lower. 3. The treatment was divided into stage of attack and remission stage. There was removing heat from the lung and resolving sputum with tonifying the kidney, keeping the inspired air going downward and resolving sputum with warming the kidney and reinforcing yang, ventilating the lung and resolving sputum to relieve asthma with invigorating the spleen and warming the kidney for treatment of the stage of attack. There was tonifying the lung and energy and nourishing yin, replenishing vital essence and supporting yang to tonify the kidney, warming and tonifying of the spleen and the kidney, strengthening the spleen and replenishing qi for treatment of remission stage. 4. The prescription of Geriatric asthma was as follow; Sam Ja Yang Chin Tang (treating superficiality), Geum Guae Sin Qi Hwan(treating origin), So Ja Gang Qj Tang, Saeng Maek San Hab Bo Pae Tang, Bo Pae A Gyo Tang, Maek Mun Dong Tang, In Sam Bo Pae Hwan, Sam Ja Jung Won Eum, Hab Gae Yang Pae Hwan, Suh Yeo Nab Qj Tang. Haeng Sam San(treating both the principal and the secondary aspects of a disease) etc. 5. In prescription, conservation medicine was most predominent(44%) followed by expectorant medicine, dispersive medicine in turn.

폐기종(肺氣腫)의 한의학적(韓醫學的) 병인(病因) 병리(病理) 및 치료(治療)에 관(關)한 문헌적(文獻的) 고찰(考察)

서운교,정지천,Seo, Woon-Kyo,Jeong, Ji-Cheon 대한한방내과학회 1995 大韓韓方內科學會誌 Vol.16 No.1

Study on Pulmonary emphysema based on literature and thesis, etc. Knowing the cause of the oriental medicine and treatment. The results were obtained as follows: 1. In the oriental medicine, we could find that emphysema was similiar to Pye-Chang, Huh-Chun. Sang-Qi, Dan-Qi, So-Qi. 2. The cause was divided to asthenia of the lung and kidney, yang-asthenia of the spleen and kidney with energy-asthenia of the lung as the root, and sputum(痰) had been important in the early period of disease, extravasated blood in the latter period. The proximate cause was clonic pulmonary disease, smoking, air pollution. occupation and symptoms of senility, congenital cause etc. 3.The treatment was divided to Gang-Qi-Wha-Dam, Whal-Hyul-Wha-Eo, Jin-Hae-Pyung-Chun in progress of disease and it was divided to Geon-Bi, Nab-Qi, On-Yang, Yang(養)-Eum, Qi-Eum-Ssang-Bo in relieve period. 4.The medicine used to Bu-Bi-Seng-Maek-San, Jo- Jung-Ik-Qi-Tang, Jung-Won-Eum, Geum-Guae-Sin-Qi-Whan, Jin-Mu-Tang, Yuk-Mi-Whan and So-ja-Gang-Qi-Tang, Pae-Mo-Tang, Ja-Won-Tang, Do-Dam-Tang, Chun-min-Tang, Jeon-Ho-Tang etc. The popular used medicine used to Bo-Pae-Tang, Pyung-Chun-Go-Bon-Tang, In-Sam-Hap-Gae-San-Ga-Gam, In-Sam-Yun-Pae-Won, Jung-Chun-Tang, Bo-Shin-Lee-Pae-Tang etc. Exogenous pathogenic fact and increasing of symptom used to Sam-So-Eum, Sang-Gook-Eum, Wol-Bi-Ga-Ban-Ha-Tang, Sa-Baek-Tang, Ma-Hwang-Tang etc.

구맥(瞿麥)의 항돌연변이(抗突然變異) 활성(活性)에 관한 연구(硏究)

서운교,정지천,Seo Un-Kyo,Jeong Ji-Cheon 대한한의학방제학회 2003 大韓韓醫學方劑學會誌 Vol.11 No.2

Antigenotoxicity test (SOS chromotest) and antimutagenecity test (Ames test) were carried out using water-soluble and methanolic extracts from Dianthi Herba. Antigenotoxic activity of methanolic extract against mutagens both MNNG and NQO was much more effective than that of water-soluble one. When the extract was added to the certain concentration $(100\;{\mu}l/tube)$, antigenotoxic activities against both mutagens were enhanced. Against the mutagen MNNG with Ames test, antimutagenic activity of the methanolic extract was better than that of water-soluble one. The 74.6% of inhibition ratio for revertant forming CFU/plate was shown at $300\;{\mu}l/plate$ of the methanolic extract.

북강활 에틸아세테이트분획의 비만세포에서의 염증반응 억제효과

서운교 ( Un Kyo Seo ),이주일 ( Ju Il Lee ),박준홍 ( Jun Hong Park ),박용기 ( Yong Ki Park ) 대한본초학회 2008 大韓本草學會誌 Vol.23 No.4

Objectives: In this study, the pharmacological effects of the ethylacetate extract of Ostericum koreanum(North Kangwhal; NK) on allergic inflammation were investigated in activated human mast cells. Methods: North Kangwhal was extracted with 80% methanol for 24 h, and then fractionated with ethylacetate(NK-EtOAc extract). HMC-1 cells, an human mast line, were pre-incubated with different concentrations of NK-EtOAc extract for 30 min, and then stimulated with PMA(50 nM/ml) and A23187(1 μM/ml) at indicated times. The cell toxicity was determined by MTT assay. The concentrations of prostaglandin E2(PGE2) and cytokines(TNF-α, IL-8) were measured by enzyme-linked immunosorbant assay. Results: NK-EtOAc extract(10~50 ㎍/ml) significantly inhibited the productions of PGE2, TNF-α and IL-8 in PMA/A23187-stimulated HMC-1 cells without cell toxicity(0~50 ㎍/ml). NK-EtOAc extract also inhibited PMA/A23187-induced phosphorylation of ERK1/2 MAPK and the NF-κB p65 subunit translocation into the nuclear of HMC-1 cells. Conclusions: This study suggests that NK-EtOAc extract may have an anti-inflammatory property through suppressing the production of inflammatory mediators in activated mast cells and its molecular mechanism underlies the blocking of NF-κB pathway.

생강 클로로포름 분획의 활성화된 뇌신경교세포(腦神經膠細胞)에서 염증반응 억제효과

서운교 ( Un Kyo Seo ),정효원 ( Hyo Won Jung ),박용기 ( Yong Ki Park ) 대한본초학회 2008 大韓本草學會誌 Vol.23 No.3