비만증 환자에서 한약제에 의한 체중 감소효과

Oh, Seung-Joon,Jeong, In-Kyung,Kim, Young-Seol,Choi, Young-Kil,Paeng, Jeong-Ryung,Bae, Jung-Hwan,Shin, Hyun-Dae 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Seung-Joon Oh, In-Kyung Jeong, Young-Seol Kim, Young-Kil Choi, Jeong-Ryung Paeng¹, Jung-Hwan Bae and Hyun-Dae Shin²Department of internal Medicine, College Medicine, Endocrine Research Institute¹. Department of Rehabilitation, College of Oriental Medicine², Seoul, korea. Bady Fat Reduction Effects of Red Ginseng Compound Preparation on the Patients with Obesity. Proceedings of International Symposium on East-West Medicine, Seoul. 244-254, 1999.-Obesity can be defined as a metabolic disease due to an increased state of fat tissues caused by an imbalance of calorie intake and use. Recently, in Korea by improvement and westernization of food intake, along with decrease in exercise activities, the prevalence of obesity has increased greatly. Our objectives were to study stability and effects of decrease in body fat by administering red ginseng compound preparation (known to have body fat decreasing effects in laboratory animals) to obesity patients on low calorie diets. Changes in weight and body fat were measured while carrying out calorie-restricted diets on patients for 4 weeks, then administering red ginseng compound preparation for another 4 weeks. The patients were 20 people whose BMI were 25kg/㎡ or over and whose percent body fat was also 30% or over when tested by bioelectrical conductivity. 1. Changes in weight were from 70.04kg(base line) to 67.43kg(after taking red ginseng compound preparation). 2. In similar sense, BMI decreased from 27.12kg/㎡(base line) to 26.56kg/㎡(after dieting), and further to 26.01kg/㎡ (after taking red ginseng compound preparation). The BMI seemed to decrease significantly compared to the baseline after the use of red ginseng compound. 3. Waist hip ratio was changed from 0.8858(base line) to 0.8728, but it was statistically insignificant. 4. The percent body fat was 35,16%(base line), 33.87%(after dieting), and 31.68%(after taking red ginseng compound preparation). 5. Complete blood cell count and blood chemisrty remained unaffected by the administration. 6. In concern to endocrinologic studies, T3 decreased from 118.7 to 98.2ng/dL, and T4 increased from 8.8 to 9.2㎕/ dL. Epinephrine showed a tendency to decreased from 0.27 to 0.25 ng/mL, and norepinephrine increased from 0.39 to 0.44ng/mL. 7. Leptin was not changed. 8. Some patients complained adverse effects; constipation(5 patients, may be due to diet therapy), fatigue (2 patients), pruritus(2 patients), flushing(s patients), dizziness(3 patients) and epigastric discomfort(2 patients). However their symptoms were mild, so medication did not stopped. In conclusion, loss of weight without significant side effects was observed during low calorie diet and red ginseng compound preparation administration. This is thought to be in relation to sympathetic nerve system rather than adrenal gland. Also, further long0term studies should be required, since the observed results were based on short-term changes in weight.

제초제 2,4-D가 흰쥐 소장 점액질에 미치는 영향에 관한 조직화학적 연구

신인성,정길남,조운복 부산대학교 사범대학 과학교육연구소 2007 科學敎育硏究報 Vol.34 No.-

제초제인 2,4-D가 십이지장샘, 십이지장, 회장 및 공장 술잔세포내 점액질에 미치는 영향을 규명하기 위하여, 성숙한 흰쥐에 제초제 2,4-D 액제를 경구적으로 투여하며 중독시킨 후 3시간군, 6시간군, 12시간군, 24시간군, 48시간군, 72시간군, 96시간군 및 120시간군 별로 나누어 점액샘 및 술잔세포내 점액질 성상을 alcian blue pH 2.5 염색법, alcian blue pH 1.0 염색법, PAS 염색법, alcian blue pH 2PAS 염색법, alcian blue pH 1.0-PAS 염색법 및 aldehyde fuchsin pH 1.7-alcian blue pH 2.5 염색법으로 검색하여 관찰하였다. 십이지장샘내 중성점액질 양은 농약투여 후 12시간군부터 96시간군까지 현저한 감소를 보였으며, 감소된 중성점액질 양은 농약투여 후 120시간군에서 회복되었다. 농약투여 후 소장 술잔세포내 점액질 감량은 음모상부 및 은와저부 쪽에서 더 심하였고, 산성점액질의 감량이 중성점액질의 감량보다 매우 더 심하였고, 회복도 산성점액질보다는 중성점액질쪽에서 더 빨랐다. 장기별로는 십이지장 술잔세포가 제일 심한 영향을 받았고, 다음 공장 배상세포이었으나 회장에서는 현저하지 않았다. 소장에 있어서 산성점액질의 가장 현저한 감소는 십이지장에서 농약투여 추 12시간군, 24시간군 및 48시간군 또는 공장에서 농약투여 후 24시간군과 48시간군이었으며 현저한 감소는 십이지장에서 농약투여 추 3시간군과 6시간군, 공장에서 농약투여 후 12시간군 그리고 다소 현저한 감소는 회장에서 농약투여 후 12시간군, 24시간군, 48시간군 및 72시간군이었다. 감소된 산성점액질 양은 십이지장 및 공장에서 농약투여 후 72시간군 또는 회장에서 농약투여 후 96시간군에서 점차적으로 회복되는 경향을 나타내었다. 중성점액질 양의 감소는 십이지장에서만 농약투여 후 12시간군, 24시간군 및 48시간군에서 현저한 감소를 나타내었으나 공장 및 회장에서는 중성점액질의 감소가 현저하지 않았으며, 감소된 중성점액질 양은 농약 투여 후 십이지장 및 공장에서 72시간군 또는 회장에서 96시간군에서 점차 회복되는 경향을 나타내었다. 소장융모 및 은와내 강 sulfated mucin을 분비하는 술잔세포와 sialomucin을 분비하는 배상세포가 농약투여 후 다같이 그 염색성이 감희되었고, 전자가 후자보다 더 심한 영향을 받았으며 회복되는 경향도 늦었다. 특히 공장에서는 sialomucin을 분비하는 술잔세포가 농약투여 후 12시간군, 24시간군 및 48시간군에서 증가되는 경향을 나타내었다. This experiment was performed to observe the effect of herbicide, 2,4-D, on the production, secretion and histochemical properties of the mucosubstances of the duodenal glands and goblet cells in the small intestine. Healthy adult rats weighing about 250~300g were divided into two groups normal and experimental. Each animal of experimental groups was administrated 2,4-D (187.5mg/kg) orally and sacrificed in 3, 6, 12, 24, 48, 72, 96 and 120 hours after 2,4-D administration. The mucous glands and the goblet cells of the small intestine were stained with alcian blue(AB) PH 2.5, AB pH 1.0, periodic acid Schiff (PAS), AB pH 2.5-PAS, AB pH 1.0-PAS and aldehyde fuchsin AF pH 1.7-AB pH 2.5. The obtained results were as follows : Marked diminution of neutral mucosubstances in the duodenal glands was noted up from 12 hours to 96 hours, and recovered gradually from 120 hours after 2,4-D administration. Decrease of both acid and neutral mucosubstances was noted to be considerable in the goblet cells of the small intestine after 2,4-D administration, especially of the upper willi and the basal regions of the crypts, and add mucins became more markedly diminished than neutral ones, while neutral mucins more strikingly recovered than add ones. In comparison with organs, e goblet cells of duodenum seemed to be most strikinly affected, next those of the ileum, but those of jejunum not affected markedly. The most remarkable decrease of acid mucosubstances was observed in the duodenum 12, 24 and 48 hours, in the jejunum 24 and 48 hours. Remarkable decrease of those observed in the duodenum 3 and 6 hours, in the jejunum 12 hours. Slight decrease of those observed in the ileum 12, 24,m 48 and 72 hours after 2,4-D administration. the decreased amount of acid mucins tended to be gradually recovered in the duodenum and jejunum 72 hours, in the ileum 96 hours after 2,4-D administration. The amount of neutral mucins was markedly diminished only in the duodenum 12, 24 and 48 hours after the administration, but neutral mucins was not diminished in the jejunum and ileum. The decreased amount of neutral mucins tended to be gradually recovered in the duodenum and jejumin 72 hours and in the ileum 96 hours after the administration. Stainability of the goblet cells with strong sulfated mucins and with sialomucins both in the intestinal villi and crypts was generally decreased after the administration. However, the former was more markedly affected than the latter. The tendency of recover appeared to be slow, especially the goblet cells with sialomucins in the jejunum 12, 24 and 48 hours tended to be increased after the administration.

Four Taeniasis saginata Cases Diagnosed at a University Hospital in Korea

Eun Jeong Won,신주현,이유정,김문주,강승지,Sook In Jung,Soo Hyun Kim,Jong Hee Shin,채종일,신성식 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.3

In recent years, the taeniasis has been rarely reported in the Republic of Korea (Korea). But in this study, we in- tend to report 4 taeniasis cases caused by Taenia saginata during a 5-month period (February to June 2018) at a unversity hospital in Gwangju, Korea. Worm samples (proglottids) discharged from all cases were identified by phenotypic and mo- lecular diagnostics. Mitochondrial cytochrome c oxidase subunit I sequences showed 99.4-99.9% identity with T. sagina- ta but, differed by 4% from T. asiatica and by 7% from T. multiceps, respectively. We found that tapeworms in 2 cases (Cases 2 and 3) yielded exactly the same sequences between them, which differed from those in Cases 1 and 4, sug- gesting intra-species variation in tapeworms. These taeniasis cases by T. saginata infection in this study, which occurred within a limited time period and region, suggest the possibility of a mini-outbreak. This study highlights the need for fur- ther epidemiological investigation of potentially overlooked cases of T. saginata infection in Korea.

뇌경색 환자의 혈중 호모시스틴치 (예비 연구)

정두신,송인욱,조상걸,성기범,박형국,신현길,장성근 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

靈光地域에 分布한 쥬라기 花崗岩類의 岩石化學的 硏究

金熙南,申仁鉉,鄭煥基 조선대학교 기초과학연구소 1988 自然科學硏究 Vol.11 No.1

The studied area is located in the part of Youngkwang, Chollanamdo, Which is in the Southwestern part of the Ogcheon Geocynclinal zone. The geology consists of the Cretaceous volcanic rocks lie in unconformity, on which the Jurassic granitoids intruded themselves into age-unknown the schist in Ogcheon system. The granitoids consists of Hornblende biotite granite, Biotite granite, Porphyritic biotite granite, Two mica granite and fine grained muscovite granite. The methods of study are as follows:80 rock samples were picked up around the out-crops, and classified according to the distributed areas and there kinds. 32 thin section out of 80 samples were a modal analysis. And 5 out of the samples were chemical analysis. As a result of the modal analysis, the granitoids in the studied area are plotted by granite and quartz monzodiorite and have the similarity of the C-zone older group in Ogcheon system. From the result of the chemical analysis, the Jurassic granitoids are similarity C-zone older group in the Ogcheon system but are poor CaO and MgO. These granitoids belong to the alkali-calcic rock series in the a1kali-lime index composed by Peacock. By the I-S type classification by white and Chappell (1977) and Hine (1978), these granitoids originated from magmas. Measuring the magnetic susceptibilities through 44 rock samples resulted in predominated magnetite series. The proportion of the alk: fm of the Niggli mean value is 13:19, and these granitoids were possible generated from before the C-zone older group in Ogcheon system.

P255 : Relationship between the HLA-G 14bp insertion/ deletion polymorphism and susceptibility to autoimmune disease: a mata-analysis

( June Hyuck Yim ),( Tae In Kim ),( In Jung Kang ),( Ki Heon Jeong ),( Su Kang Kim ),( Joo Ho Chung ),( Min Kyung Shin ),( Mu Hyoung Lee ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Numerous studies have investigated the potentialrelationship of human leukocyte antigen (HLA)-G 14bp insertion/deletion (INS/DEL) polymorphism with autoimmune disease (AID). However, results from published data were inconclusive. Objectives: Our aim in this study was to determine whether the 14bp INS/DEL polymorphism in the HLA-G gene contributes to the risk of AID. Methods: A systemic literature search of PubMed and Embase databases was conducted to identify eligible studies investigating the association of HLA-G 14bp INS/DEL polymorphism with AID. The final analysis included 13 publications with a total of 6462 individuals. Results: Overall, no significant association between HLA-G 14-bp INS/DEL polymorphism and overall AID was detected in all comparison models. Further subgroup analyses based on AID types and ethnicity showed no significant association. Conclusion: Our results suggest that the HLA-G 14bp INS/DEL polymorphism might not be related to the development of AID. Further studies with larger sample size are warranted to validate our finding.

Risk mitigation strategy by Passive IN-core Cooling system for advanced nuclear reactors

Seo, Seok Bin,Kim, In Guk,Kim, Kyung Mo,Jeong, Yeong Shin,Bang, In Cheol Elsevier 2018 Annals of nuclear energy Vol.111 No.-

<P><B>Abstract</B></P> <P>After the Fukushima accident, the installation of passive safety systems for nuclear power plants becomes necessary as protection against external power failures. Recently, an innovative safety system was introduced by employing a hybrid heat-pipe concept asa Passive IN-core Cooling system (PINCs). The hybrid heat-pipe concept employs the combination of a control rod and a heat pipe to passively shutdown a reactor in an accident condition and simultaneously remove decay heat from the core. As a result, a PINCs can handle the entire accident sequence in a single system, leading to significant enhancement in nuclear reactor safety. In this study, the safety enhancement of various nuclear reactors has been quantitatively evaluated by using the probabilistic safety assessment method (PSA). PSA models of the APR1400, PGSFR, and SMART nuclear reactors developed in Korea, are developed, and a PINCs model has been applied to each reactor. The PSA model of the PINCs has been developed based on the systematic design and configuration of each reactor. In addition, postulated accident sequences where the PINCs operates have been developed in the PSA model. Finally, the consequence of each accident sequence has been quantitatively evaluated in the form of core damage frequency (CDF) and minimal cut set (MCS) for each nuclear reactor. Based on the PSA results, it is confirmed that the application of the PINCs effectively reduces the overall CDF of all nuclear reactors. In addition, the failure paths causing the core damage are restricted for APR1400 and PGSFR. In conclusion, the employment of the PINCs significantly improved the overall safety degrees of various nuclear reactors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PINCs combines a control rod and a heat pipe to enhance safety of advanced reactors. </LI> <LI> PSA models of advanced reactors are developed including PINCs. </LI> <LI> The consequences of the accident aided by PINCs are quantitatively evaluated. </LI> <LI> PINCs significantly reduces the overall CDFs of all nuclear reactors. </LI> </UL> </P>

법랑모세포 분화와 성숙과정에서 OD314의 발현

박주철,안성민,김흥중,정문진,박민주,신인철,손호현 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.5

법랑모세포는 법랑질을 형성하고 유지하는 세포로, 법랑질의 유기기질을 분비하고 법랑질 석회화 과정에도 관여한다. 치아 발생과정에서 법랑모세포의 분화는 순차적인 상피-간엽 상호작용에 의하여 조절되나, 분화나 성숙과정의 정확한 기전은 아직까지 잘 알려져 있지 않다. 최근에 상아모세포에서 처음 발견된 OD314가 치아 발생과정에서 상아질을 형성하는 상아모세포 뿐 아니라 법랑모세포에도 발현된다고 하였다. 이에 본 연구에서는 생쥐 하악 전치의 다양한 시기의 법랑모세포를 이용하여, 형태학적 분석과 in-situ hybridization에 의한 OD314 mRNA의 발현 그리고 OD314 항체를 이용한 면역조직화학적 분석을 통하여 OD314 유전자의 법랑 모세포 분화와 성숙과정에서의 역할을 연구하여 다음과 같은 결과를 얻었다. 1. 형태학적으로 법랑모세포는 분화 단계에 따라 분비 전단계 법랑모세포, 분비기 법랑모세포, 성숙기의 평탄끝 법랑모세포와 성숙기의 주름끝 법랑모세포로 구분되었다. 2. OD314 mRNA는 분비기의 법랑모세포에서부터 발현되기 시작하여 법랑모세포가 성숙해갈 수록 그 발현이 증가하였다. 3. OD314 단백질은 분비 전단계의 법랑모세포에서는 발현되지 않고, 분비기의 법랑모세포에서는 세포질에 전체적으로 발현되었다. 성숙기의 평탄끝 법랑모세포와 주름끝 법랑모세포에서는 세포의 근심과 원심끝단에 OD314 단백질이 강하게 발현되었다. 이상의 결과를 종합하여 OD314는 법랑모세포의 분화와 성숙과정에서 세포질 내부에서 특징적인 역할을 하는 것으로 사료된다. Ameloblasts are responsible for the formation and maintenance of enamel which is an epithelially derived protective covering for teeth. Ameloblast differentiation is controlled by sequential epithelial-mesenchymal interactions. However, little is known about the differentiation and maturation mechanisms. OD314 was firstly identifled from odontoblasts by subtraction between odontoblast/pulp cells and osteoblast/dental papilla cells, even though OD314 protein was also expressed in ameloblast during tooth formation. In this study, to better understand the biologcal function of OD314 during amelogenesis, we examined expression of the OD314 mRNA and protein in various stages of ameloblast differentiation using in-situ hybridization and immunohistochemistry. The results were as follows : 1. The ameloblast showed 4 main morphological and functional stages referred to as the presecretory, secretory, smooth-ended, and ruffle-ended. 2. ○D314 mRNA was expressed in secretory ameloblast and increased according to the maturation of the cells. 3. OD314 protein was not expressed in presecretory ameloblast but expressed in secretory ameloblast and maturative ameloblast. OD314 protein was distributed in entire cytoplasm of secretory ameloblast. However, OD314 was localized at the proxiamal and distal portion of the cytoplasm of smooth- ended and ruffle-ended ameloblast. These results suggest that ○D314 may play important roles in the ameloblast differentiation and maturation.

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

법랑모세포 분화와 법랑질 형성과정에서 OD314, Apin protein의 발현 및 기능

박종태,최용석,김흥중,정문진,오현주,신인철,박주철,손호현 대한치과보존학회 2006 Restorative Dentistry & Endodontics Vol.31 No.6

본 연구에서는 법랑모세포 분화와 법랑질 형성에 연관이 있는 OD314 일명 Apin protein의 기능을 밝힐 목적으로, in-situ hybridization에 의한 OD314 mRNA 발현과 법랑모세포 세포주에서 OD314 enamel matrix protein의 발현, 그리고 OD314 유전자를 과발현/억제시킬 수 있는 construct를 제작한 후 법랑질 형성 중에 OD314의 기능을 알아보고자 RT-PCR를 시행하여 다음과 같은 결과를 얻었다. 1. OD314 mRNA는 발생중인 상아모세포보다 법랑모세포에서 강하게 발현되었다. 2. Tuftelin은 석회화 결정이 형성되는 14일까지 발현이 지속되고, 그 이후부터 점차 감소하였다. Amelogenin과enamelin은 7일부터 그 발현이 점점 감소하였다. 3. U6-OD314 siRNA construct를 이용하여 transfection한 법랑모세포 세포주는 OD314와 tuftelin,MMP2 mRNA 발현이 감소하였으며, CM-OD314를 transfection하여 OD314의 과발현을 유도한 경우에는 OD314와 MMP20 mRNA의 발현이 뚜렷이 증대되었다. 이 결과는 OD314가 법랑모세포의 분화와 법랑질의 형성 그리고 석회화 과정에 중요한 역할을 하는 새로운 인자임을 시사한다. This study was aimed to elucidate the biological function of OD314 (Apin protein), which is related to ameloblast differentiation and amelogenesis. Apin protein, calcifying epithelial odontogenic (pindborg) tumors (CEOTs)-associated amyloid, were isolated from CEOTs, and has similar nucleotide sequences to OD314. We examined expression of the OD314 mRNA using in-situ hybridization during tooth development in mice. Expression of OD314 and several enamel matrix proteins were examined in the cultured ameloblast cell line up to 28 days by reverse transcription-polymerase chain reaction (RT-PCR) amplification. After inactivation and over-expression of the OD314 gene in ameloblast cell lines using U6 vector-driven RNA interference and CMV-OD314 construct, RT-PCR were performed to evaluate the effect of the OD314 during amelogenesis. The results were as follows: 1. In in-situ hybridization, OD314 mRNAs were more strongly expressed in ameloblast than odontoblast. 2. When ameloblast cells were cultured in the differentiation and mineralization medium for 28 days, the tuftelin mRNA expression was maintained from the beginning to day 14, and then gradually decreased to day 28. The expressions of amelogenin and enamelin were gradually decreased according to the ameloblast differentiation. 3. Inactivation of OD314 by U6-OD314 siRNA construct down-regulated the expression of OD314, MMP-20, and tuftelin, whereas over-expression of OD314 by CMV-OD314 construct up-regulated the expression of OD314 and MMP-20 without change in tuftelin. These results suggest that OD314 is considered as an ameloblast-enriched gene and may play the important roles in ameloblast differentiation and mineralization.