Allergy성(性) 접촉피부염(接觸皮膚炎)에 대(對)한 고삼(苦蔘), 황연(黃連)의 Iontophoresis 치료(治療) 효과(效果)

김찬중,구영희,남혜정,김윤범,Kim, Chan-Jung,Ku, Young-Hui,Nam, Hae-Jeong,Kim, Yoon-Bum 대한한방안이비인후피부과학회 2005 한방안이비인후피부과학회지 Vol.18 No.1

Objective : This study was performed to investigate the effects of Sophorae Radix and Coptidis Rhizoma-iontophoresis in allergic contact dermatitis. Methods : Contact hypersensitivity assay, video microscope, melanin-erythema levels, pH levels, hydration levels, WBC count, RBC count, neutrophil ratio, lymphocyte ratio and total IgE levels were measured. Results : At contact hypersensitivity assay, the right ear swellings in Sophorae Radix and Coptidis Rhizoma-iontophoresis group were decreased compared with control group, but have no statistical significance. At observation of skin morphologic change, many papules were seen in control group and keratins were seen in Sophorae Radix-iontophoresis group. At melanin levels, Sophorae Radix and Coptidis Rhizoma-iontophoresis groups showed no significant difference compared with control group. At pH levels, Sophorae Radix-iontophoresis group showed significant decreased with control group. At hydration levels, Sophorae Radix and Coptidis Rhizoma-iontophoreses groups showed to significant difference compared with control group. WBC count, RBC count and neutrophil ratio were significantly increased in Sophorae Radix and Coptidis Rhizoma-iontophoresis group compared with control group. Lymphocyte ratio and total IgE levels were significantly decreased in Sophorae Radix and Coptidis Rhizoma-iontophoresis groups compared with control group. At morphology of skin, inflammation was decreased and the thickness of epidermis was well preserved in Sophorae Radix and Coptidis Rhizoma-iontophoresis group. Conclusion : Sophorae Radix and Coptidis Rhizoma-iontophoresis had some anti-allergy, anti-inflammatory effects on allergic contact dermatitis, but had no effects on melanin-erythema levels, pH levels and hydration levels of skin.

黃連과 구성 생화합물의 약리작용에 대한 고찰

김기배(Ki Bae Kim),이형탁(Hyung Tak Lee),구경회(Kyung Howi Ku),홍진우(Jin Woo Hong),조수인(Su In Cho) 대한한의학회 2012 대한한의학회지 Vol.33 No.3

황련(黃連)의 독성(毒性)과 부작용에 관한 문헌적 고찰

김태형 ( Tae Hyung Kim ),노성수 ( Seong Soo Roh ),구진숙 ( Jin Suk Koo ),서부일 ( Bu Il Seo ) 한약응용학회 2018 한약응용학회지 Vol.18 No.1

Coptidis Rhizoma is in use in the Korean medicine. The Korean medical doctors use Coptidis Rhizoma for clearing away heat and dehydrating dampness(淸熱燥濕), eliminating toxin(解毒), purging the pathogenic fire(瀉火), etc. We investigated toxicity, poisoning symptoms, side effects, poisoning treatment and prevention against poisoning of Coptidis Rhizoma. The results were summarized as follows: 1. The main ingredients of poisoning are berberine, etc. The ingredients of poisoning from Coptidis Rhizoma mainly can cause stimulating symptoms on respiratory system, circulatory system, digestive system, nervous system, blood system, etc, and can cause allergic reaction. 2. The representative methods of poisoning treatment in the western medicine are supplies of grape sugar, antianaphylactic treatment and symptomatic treatment, etc. 3. In order to prevent against poisoning of Coptidis Rhizoma, the patients should keep usage, dosage and notes.

黃連-吳茱萸 藥對의 활용에 대한 고찰

조혜인,국윤범,Joh, Hae-In,Kook, Yoon-Bum 대한한의학방제학회 2015 大韓韓醫學方劑學會誌 Vol.23 No.2

Objectives : This study is intended to establish the areas of use of the major effects of Coptidis Rhizoma-Evodiae Fructus Pair in the Prescriptions. Methods : Prescriptions that include both Coptidis Rhizoma and Evodiae Fructus have been selected with the use of programs offered by <uri>http://www.koreantk.com, http://youl.net/data</uri> . This study compared and analyzed the main effects of the selected prescriptions. Results : Prescriptions that work on upper digestive system tend to have Coptidis Rhizoma to Evodiae Fructus ratio of 2:1~10:1, and Prescriptions that work on lower digestive system tend to have ratio of 1:1. When Coptidis Rhizoma takes up 50% or more of the total dose of the prescriptions, these prescriptions tend to have major effect on the upper digestive system, and when it takes less than 50%, these tend to have major effect on the lower digestive system on the contrary. Conclusions : The prescriptions based on Coptidis Rhizoma-Evodiae Fructus Pair have a tendency to treat digestive problems caused by mass of fever in Liver-system. On this basis, further studies are expected to unveil the effectiveness of these prescriptions on adult diseases.

Effect of Chitosan and Tannin Treatment on the Functional Manifestation of Coptidis Rhizoma Dyed Fabrics

( Soo Jin Ryu ),( Hyun Sook Bae ) 한국의류학회 2018 한국의류학회지 Vol.42 No.6

Lifestyle of Health and Sustainability (LOHAS) influences the development of hygienic and health-oriented functional textile products; consequently, there has been an increase in the prospects for environmentally friendly natural dyeing products with functional and sensitive characteristics. Therefore, the present study expresses a functional manifestation on fabrics by treatment with chitosan and tannin using natural Coptidis Rhizoma. Cotton and wool fabrics dyed with Coptidis Rhizoma after treatment with chitosan and tannin. Consequently, the antibiosis of fabric dyed with Coptidis Rhizoma was excellent with 99.9% bacterial reduction for Staphylococcus aureus and Klebsiella pneumoniae in both fabrics. The deodorization rate was more than 90.0% in both fabrics after 120 minutes. The UV-A protection rate was 88.2% and the UV-B protection rate was 87.7% for cotton. The UV-A protection rate was 88.2% and the UV-B protection rate was 84.3% for wool fabric. Therefore, both of the dyed fabrics showed excellent UV protection by treatment with only chitosan and tannin, without mordants. Therefore, chitosan and tannin treatments were able to manifest functionalities in the fabrics dyed with Coptidis Rhizoma.

황련에서 분리된 단백질성분의 항진균효과

김현경(Hyunkyung Kim),이주희(Jue Hee Lee),심진기(Jin Kie Shim),한용문(Young Moon Han) 대한약학회 2005 약학회지 Vol.49 No.4

Antimicrobial peptides are evolutionary ancient weapons for animal and plant species to depend themselves against infectious microbes. In the present study, we investigated if an antimicrobial peptide was produced from Coptidis Rhizoma. For the determination, protein substance from the medicinal plant was isolated by various preparations. Among the preparations, the protein portion dissolved in phosphate-buffered saline solution (CRP-DS) that contained the most amount of protein (90%) resulted in maximal inhibition of Candida albicans which causes local and systemic infections. Analyses by gel-electrophoresis and gel-permeation chromatography showed the CRP-DS formed a single band of approximately 11.8 KDa as molecular size. Antifungal activity of the CRP-DS was almost equivalent to antifungal activity by fluconazole, resulting in MIC (minimal inhibitory concentration) of approximately 50 ㎍/㎖. The antifungal activity was a dose-dependent. The antifungal activity appeared to be inactivated by heat-treatment and ionic strength, respectively. In a murine model, the CRP-DS enhanced resistance of mice against disseminated candidiasis. The HPLC analysis demonstrated maximum 4% of berberine as residual content in the CRP-DS preparation resulted in no influence on the antifungal activity. In addition, protein portion isolated from Phellodendri Cortex producing the alkaloid component like Coptidis Rhizoma had no such anticandidal effect. These results indicate that the protein substance from Coptidis Rhizoma was responsible for the antifungal activity.

黃連 製劑 中 protoberberine의 分析에 관한 硏究

이내홍,김영일,엄동옥 우석대학교 의약품개발연구소 1997 藥學硏究誌 Vol.2 No.-

Tetrathiocyanatocobaltate(Ⅱ) ion is capable to complex compounds with tertiary amine salts or quarternary ammonium salts. And these complex compouds. no soluble in water, are extracted into organic phase, the same as 1,2-dichloroethane, chloroform and dichloromethane. We adopted the method for determination of protoberberine in the preparations of Cottidis Rhizoma(Samhwangsasim-tang. Hwangryunhaedog-tang and Banhasasim-tang). Extracted complex compounds, in organic phase, was identified and determinated by U.V spectrophotometry. The maximum absorption wave lengths of the complex compounds were 442nm and 624nm. At 624nm as one of the maximal absorbances, it was copable to determinate the content of protoberberine. The calibration curve was liniary at the range of 0.05mg/ml-0.4mg/ml of berberine chloride in DCE. The contents of protoberberine its derivatives were also determinated. On the other hand, it was suggested that a certain reaction among the various compounds of the different Herbe medicals. It could be explained the diminution of the protoberberine content for the preparations of Coptidis Rhizoma. It was considered that spectrophotometry method could be greatly applied to the analysis of protoberberine in the various preparations of Coptidis Rhizoma and its in the industrial laboratory. Also this method could be adopted for the determination of other alkaloids which consist amine and ammonium.

키토산과 탄닌처리에 의한 황련염색직물의 염착거동 변화

류수진 ( Soo Jin Ryu ),배현숙 ( Hyun Sook Bae ) 한국의류산업학회 2018 한국의류산업학회지 Vol.20 No.2

The natural dye product market is expanding due to the recent interest in environmentally friendly products. This study examines dyeing using natural Coptidis Rhizoma. Chitosan and Tannin was treated to improve dyeability of Coptidis Rhizoma after finding the proper dyeing condition. In addition, dyeing characteristics were compared according to mordant types along with the mordanting methods for dye fixing and color change. The results indicated that the optimum dyeing condition was to treat the concentration of 5% (o.w.b.) at 80oC for 90 minutes. By the chitosan and tannin treatments, K/S value of cotton fabrics by 3 times and those of wool fabrics by 2 times increased and color depthing of dyed fabrics was achieved after 2 cycles repetition. To improve dyeability, iron mordanting was most effective; in addition, the K/S Value of pre-mordanting fabrics versus post-mordanting fabrics increased the most. The color of the surface was changed to reddish yellow when Sn, Cu mordanting, and to greenish blue when Fe mordanting. The lightfastness of dyeing fabric with mordanting was weak at 1-2 grades, but the washing fastness was good for 4 grades and the rubbing fastness and sweat fastness were as good as those of the 3-4 grades. As a result, this study could help improve the dyeability of expensive Coptidis Rhizoma.

황련(Coptidis rhizoma), 오배자(Chinese galls), 피톤치드 혼합물(Phytoncide)이 비듬균(Malassezia furfur)에 미치는 영향

김정희(Joung-Hee Kim),조용권(Yong-Kwon Cho),김병관(Byung-Kwan Kim),곽승준(Seung Jun Kwack),김종국(Jong Guk Kim),이은경(Eunkyung Lee),조혜경(Hye-Kyung Cho),김극준(Keuk-Jun Kim) 한국생명과학회 2015 생명과학회지 Vol.25 No.7

본 연구는 기존의 선행 연구된 천연물 중 항균활성이 가장 좋은 황련, 오배자, 피톤치드(Phytoncide)가 단일추출물과 추출혼합물 일 때 M. furfur의 항균활성 차이에 대한 효과를 알아보고자 하였고, 선행연구에서 비듬균에 대한 천연한방추출물의 항균활성결과와 비교하였다. 추출혼합물에서 S. aureus는 3가지 혼합한 천연물질에서 항균활성이 내성하였으며 E. coli는 황련과의 혼합물에서 내성하였고 C. albicans는 오배자와 혼합물에서 내성이있는 것으로 나타나 각 균주에 따라 항균활성이 다른 것으로 나타났다. 반면 M. furfur는 단일추출물에서 전혀 항균활성이 없었고, 혼합물에서는 황련과 피톤치드 50% 혼합물에서 3.20mm의 직경으로 미약한 항균활성을 보였다. 이는 같은 진균인 C. albicans와 비교하여 볼 때 현저한 차이가 있었다. 그 이유는 M. furfur가 C. albicans에 비하여 세포벽이 지방친화성 성분을 지니고 있고 라멜라층, 원형질내막, 중간 다층구조로 되어있기 때문이라고 사료된다. The report explores the possibility that the single extract of natural substances and the mixture of single extracts of natural substances create a synergy effect to increase the antimicrobial activity. It also compares the previous researches to find out if the natural medicinal herbs" extract has antimicrobial activity on dandruff causative organism, Malassezia furfur. For the experiment on the mixture of single extracts of natural substances, the results are like following: 1. Staphylococcus aures"s antibacterial activity is resistant to mixture of three natural substances. 2. Escherichia coil"s antibacterial activity is resistant to mixture with Coptidis rhizoma. 3. Candida albicans" antifungal activity is resistant to mixture with Chinese galls, which means the different results may be expected when tested with each germ. 4. On the other hand, Malassezia furfur has no antifungal activity on the single extract of natural substances and shows weak antifungal activity, whose diameter is 3.20 mm when tested with the mixture of 50% of Coptidis rhizoma and 50% of Phytoncide. The result is totally different from the one on the same eumycetes, C. albicans. That is because M. furfur has more lipophilic chemicaled cell walls than C. albicans does and it also consists of lamella layer, inner plasma membrane and intermediate multiple layers.

Differentiating Coptis chinensis from Coptis japonica and other Coptis species used in Coptidis Rhizoma based on partial trnL-F intergenic spacer sequences

도의정,오승은,이미영,고병섭 한국유전학회 2014 Genes & Genomics Vol.36 No.3

Dried rhizomes of Coptis species are utilized as‘‘Coptidis Rhizoma’’ (CR), an important herbal medicinalmaterial in traditional Chinese medicine. Almost all CRstraded in the Korean herbal medicine market originate fromCoptis chinensis (‘‘Chun Hwang-Lyun’’ in Korean medicalterminology). Other minor CRs originate from Coptisjaponica (‘‘Il Hwang-Lyun’’). Although there is an obviousdiscrepancy in the price of traded CRs in the herbal marketdepending on the Coptis species, CRs originating from C. chinensis and C. japonica are often confused. Furthermore,the CR traded as ‘‘Chun Hwang-Lyun’’ is occasionallymixed with rhizomes of Coptis deltoidea and/or Coptisomeiensis. Therefore, we sought to discriminate C. chinensisfrom C. japonica, as well as C. deltoidea and C. omeiensis, by using nucleotide sequence differences in thepartial trnL-F intergenic spacer. We developed an efficientreal-time polymerase chain reaction (PCR)-based discriminationassay to separate samples of C. chinensis fromthose of C. japonica without the need to separate the DNAmarkers by using gel electrophoresis. In addition, wedeveloped a multiplex PCR method with which we wereable to discriminate samples of C. chinensis from those ofC. deltoidea and C. omeiensis by amplifying the 153-bpDNA marker in C. chinensis in a single PCR process.