Attenuation of Experimental Autoimmune Hepatitis in Mice with Bone Mesenchymal Stem Cell-Derived Exosomes Carrying MicroRNA-223-3p

Yong-Ping Chen,Feng-Bin Lu,Da-Zhi Chen,Lu Chen,En-De Hu,Jin-Lu Wu,Hui Li,Yue-Wen Gong,Zhuo Lin,Xiao-Dong Wang,Ji Li,Xiao-Ya Jin,Lan-Man Xu 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.12

MicroRNA-223-3p (miR-223-3p) is one of the potential microRNAs that have been shown to alleviate inflammatory responses in pre-clinical investigations and is highly encased in exosomes derived from bone mesenchymal stem cells (MSC-exosomes). MSC-exosomes are able to function as carriers to deliver microRNAs into cells. Autoimmune hepatitis is one of the challenging liver diseases with no effective treatment other than steroid hormones. Here, we examined whether MSC-exosomes can transfer miR-223-3p to treat autoimmune hepatitis in an experimental model. We found that MSC-exosomes were successfully incorporated with miR-223-3p and delivered miR-223-3p into macrophages. Moreover, there was no toxic effect of exosomes on the macrophages. Furthermore, treatments of either exosomes or exosomes with miR-223-3p successfully attenuated inflammatory responses in the liver of autoimmune hepatitis and inflammatory cytokine release in both the liver and macrophages. The mechanism may be related to the regulation of miR-223-3p level and STAT3 expression in the liver and macrophages. These results suggest that MSC-exosomes can be used to deliver miR-223-3p for the treatment of autoimmune hepatitis.

ANALYZING ISUAL SPECTROPHOTOMETER DATA USING A TWO-COLOR DIAGRAM METHOD

CHEN ALFRED BING-CHIH,CHIANG PO-SHIH,HUANG TIAN-HSIANG,KUO CHENG-LING,WANG SHI-CHUN,SU HAN-TZONG,HSU RUE-RoN,CHANG MING-HUI,CHANG YEOU-SHIN,LIU TIE-YUE,MENDE STEPHEN B.,FREY HARALD U.,FUKUNISHI HIROSH The Korean Astronomical Society 2005 Journal of The Korean Astronomical Society Vol.38 No.2

Transient luminous events (TLEs; sprites, elves, jets and etc.) are lightning-related optical flashes occurring above thunderstorms. Since the first discovery of sprites in 1989, scientists have learned a great deal about the morphological, spectroscopic and electromagnetic characteristics of TLEs through ground and spacecraft campaigns. However, most of the TLE studies were based on events recorded over US High Plains. To elucidate the possible biasing effects, space-borne observations are needed and have their merits. Imager of sprites and Upper Atmospheric Lightning (ISUAL) on the FORMOSAT-2 satellite is the first instrument to carry out a true global measurement of TLEs from a low- earth orbit. In this short paper, we apply a common astronomical data analysis technique, two-color diagram, on the ISUAL spectrophotometer (SP) data. By choosing appropriated bandpasses and converting the measured flux of TLEs into the unit of magnitude, two-color diagrams of TLEs can be constructed. We demonstrate that two-color diagrams, which were constructed from the narrow-band spectrophotometer data, can be used to classify different types of TLEs and trace their temporal evolution. The amount of reddening due to Earth's atmosphere can also be estimated from two-color diagrams assembled from the broad-band spectrophotometer data.

The infection frequencies and dynamics of three secondary endosymbionts in the laboratory environments on Sitobion avenae (Fabricius) as determined by long PCR

Chen Luo,Kun Luo,Zu-Qing Hu,Yue-Yue Tao,Hui-Yan Zhao 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.2

Stable infections of maternally transmitted secondary endosymbionts in aphids are frequently found in field populations; however,whether this phenomenon changes in artificial lab conditions has not been clear. To investigate, (1): we collected Sitobion avenae (Fabricius) fromsix different regions and detected the three common secondary endosymbionts (Regiella insecticola, Hamiltonella defensa and Serratia symbiotica); (2): we raised Sitobion avenae fromChuzhou, Anhui for 14 months in the laboratory, and analyzed these aphids' DNA using the long PCR protocol after the 1stmonth, 3rdmonth, 5th month, 7thmonth, 9thmonth, 11thmonth, 12thmonth, 13thmonth and 14th month. The results showed that S. symbiotica and R. insecticola could be detected in all aphid populations,while H. defense were detected in three locations. After reared in the lab, the infection frequencies of Serratia symbiotica did not change across the 14 study months, but the infection frequencies of Regiella insecticola after the 9th month and of Hamiltonella defensa after the 11th month decreased significantly. Additionally, the results show that facultative endosymbiont infections may decline in artificial lab conditions, suggesting that temporal changes of endosymbionts may occur within the host under different environmental pressures. This result may partially explain why different geographic populations harbor a diverse variety of endosymbionts. The results of this study can be helpful in understanding the stability of secondary endosymbionts in Sitobion avenae under long-termreproduction in controlled laboratory conditions.

Process Systems Engineering, Process Safety, Transport Phenomena : Multiloop PID Controller Design using Partial Least Squares Decoupling Structure

( Jung Hui Chen ),( Yi Chun Cheng ),( Yue Zhi Yea ) 한국화학공학회 2005 Korean Journal of Chemical Engineering Vol.22 No.2

Growth of InGaN Films on c-plane Sapphire Substrates with an AlN Nucleation Layer by Using Metal-organic Chemical-Vapor Deposition

Dang-Hui Wang,Sheng-Rui Xu,Jin-Cheng Zhang,Ke Chen,Zhi-Wei Bi,Lin-Xia Zhang,Fan-Na Meng,Shan Ai,Yue Hao 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.61 No.4

In this study, we report on the crystal quality of InGaN epifilms with different indium fractions grown at different growth temperatures on c-plane sapphire substrates with an AlN nucleation layer by using low-pressure metal-organic chemical-vapor deposition (MOCVD). High-resolution X-ray diffraction (HRXRD), atom force microscopy (AFM), photoluminescence (PL) and Raman scattering measurements were employed to study the crystal quality, optical properties and strain condition of InGaN epifilms with increasing indium fraction (from 4.36% to 15.36%). Results show that InGaN epitaxial layers can be realized with a higher indium fraction at a lower temperature by inserting an AlN nucleation layer between the sapphire substrate and the GaN buffer layer and that the obtained InGaN epifilms have an improved crystal quality and a lower threading dislocation density.

Aporphine Alkaloids from Clematis parviloba and Their Antifungal Activity

Jia-Hui Chen,Zhi-Zhi Du,Yue-Mao Shen,Yong-Ping Yang 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.1

A new aporphine alkaloid, β-magnoflorine (1), together with a known aporphine alkaloid, α-magnoflorine (2), were isolated from the aerial parts of Clematis parviloba. Their structures were elucidated on the basis of comprehensive spectroscopic techniques. In addition, both compounds showed potent antifungal activities against Penicillium avellaneum UC-4376.

JCAD deficiency attenuates activation of hepatic stellate cells and cholestatic fibrosis

Li Xie,Hui Chen,Li Zhang,Yue Ma,Yuan Zhou,Yong-Yu Yang,Chang Liu,Yu-Li Wang,Ya-Jun Yan,Jia Ding,Xiao Teng,Qiang Yang,Xiu-Ping Liu,Jian Wu 대한간학회 2024 Clinical and Molecular Hepatology(대한간학회지) Vol.30 No.2

Background/Aims: Cholestatic liver diseases including primary biliary cholangitis (PBC) are associated with active hepatic fibrogenesis, which ultimately progresses to cirrhosis. Activated hepatic stellate cells (HSCs) are the main fibrogenic effectors in response to cholangiocyte damage. JCAD regulates cell proliferation and malignant transformation in nonalcoholic steatoheaptitis-associated hepatocellular carcinoma (NASH-HCC). However, its participation in cholestatic fibrosis has not been explored yet. Methods: Serial sections of liver tissue of PBC patients were stained with immunofluorescence. Hepatic fibrosis was induced by bile duct ligation (BDL) in wild-type (WT), global JCAD knockout mice (JCAD-KO) and HSC-specific JCAD knockout mice (HSC-JCAD-KO), and evaluated by histopathology and biochemical tests. In situ-activated HSCs isolated from BDL mice were used to determine effects of JCAD on HSC activation. Results: In consistence with staining of liver sections from PBC patients, immunofluorescent staining revealed that JCAD expression was identified in smooth muscle α-actin (α-SMA)-positive fibroblast-like cells and was significantly up-regulated in WT mice with BDL. JCAD deficiency remarkably ameliorated BDL-induced hepatic injury and fibrosis, as documented by liver hydroxyproline content, when compared to WT mice with BDL. Histopathologically, collagen deposition was dramatically reduced in both JCAD-KO and HSC-JCAD-KO mice compared to WT mice, as visualized by Trichrome staining and semi-quantitative scores. Moreover, JCAD deprivation significantly attenuated in situ HSC activation and reduced expression of fibrotic genes after BDL. Conclusions: JCAD deficiency effectively suppressed hepatic fibrosis induced by BDL in mice, and the underlying mechanisms are largely through suppressed Hippo-YAP signaling activity in HSCs.

Xylazole inhibits NO-cGMP pathway in fetal rat nerve cells

Xinyu Wang,Yue Wu,Lin Liu,Hui Bai,Zhiheng Zhang,Mingchao Zhao,Tianwen Ma,Xiaopeng Song,Lina Jia,Liangyu Lv,Yue Yu,Xinyu Xu,Hong Chen,Li Gao 대한수의학회 2022 Journal of Veterinary Science Vol.23 No.1

Background: Xylazole (Xyl) is a veterinary anesthetic that is structurally and functionally similar to xylazine. However, the effects of Xyl in vitro remain unknown. Objectives: This study aimed to investigate the anesthetic mechanism of Xyl using fetal rat nerve cells treated with Xyl. Methods: Fetal rat nerve cells cultured for seven days were treated with 10, 20, 30, and 40 μg/ mL Xyl for 0, 5, 10, 15, 20, 25, 30, 45, 60, 90, and 120 min. Variations of amino acid neurotransmitters (AANTs), Nitric oxide-Cyclic GMP (NO-cGMP) signaling pathway, and ATPase were evaluated. Results: Xyl decreased the levels of cGMP and NO in nerve cells. Furthermore, Xyl affected the AANT content and Na+-K+-ATPase and Ca2+-Mg2+-ATPase activity in nerve cells. These findings suggested that Xyl inhibited the NO-cGMP signaling pathway in nerve cells in vitro. Conclusions: This study provided new evidence that the anesthetic and analgesic effects of Xyl are related to the inhibition of the NO-cGMP signaling pathway.

Chemical Stability of 2D-Nanostructured WO3 in Hydrogen Sensing under Varied Operation Temperature

Taisheng Yang,Hui Tian,Yue Zhang,Chen Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.8

Nanostructured metal oxide-based resistive-type gas sensors are of high research interest. Chemical stability is the most critical issue due to the surface electron species and density. In the present paper, 2D-nanostructured WO3 was prepared and characterized, and a WO3-based sensor was fabricated and analyzed. The results showed that the synthesized WO3 material exhibited nanosheet structure, and during hydrogen sensing testing, the current baseline shifted with various tendencies, even completely opposite directions under different operation temperatures. The chemistry analysis results indicated that water molecule and hydroxyl group were formed under low operation temperature but further oxidation occurred at higher temperatures. The adsorption of H2 on oxygen terminated WO3(0 0 1) surfaces by density functional theory (DFT) method indicated that a water molecule formed by adsorption of a hydrogen molecule at the O site with the most thermodynamically stable state, and two surface hydroxyl groups formed by dissociative adsorption with a thermodynamically less stable state. The water molecule and surface hydroxyl groups increased the conductivity of the WO3 film while that was decreased as the oxidation occurred.

Differentially Rice Protein Expression Between Rice Bran and Endosperm

( Ting-yu Chen ),( De-min Wu ),( Ji-jyun Lai ),( Chang-yue Li ),( Hui-fen Liao ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

Rice (Oryza sativa L.), the major staple food for more than 60% of the world’s population, offer nutritional and health-enhancing properties. Therefore, breeding of new rice species has been fueled by the rising interest in Asian, Latin cuisines, and many countries. In Asia, rice and rice-based ingredients also appeal to both consumers and processors due to their unique combination of taste, nutrition, texture, and biological properties. Proteins and starch in rice are the two major components in rice seed, with approximately 8 and 80%, respectively. Especially in traditional Asian diet, rice seed contributes to about 28-54% of the protein source. The major rice proteins, including structural, metabolic, protective, and storage proteins, serve as sources of nitrogen, sulfur, and carbon for several important physiological functions. In our previous study also demonstrated that rice protein prolamin activated human mononuclear cells to produce cytokines and enhance anti-leukemic immunity. The present study aimed to compare the differentially expression of rice proteins with bran and endosperm by 2-dimentional electrophoresis (2-DE) and mass spectrometric assay. Several protein spots in 2-DE gel with different expression were isolated and identified. The results showed that the major proteins were metabolic, transporter, storage, antioxidant, disease resistant, and development-related proteins. Further investigation to clarify the different manifestations and functions of these proteins might contribute to development of new rice varieties and breeding with unique features.