Studies of the microbial metabolism of flavonoids extracted from the leaves of Diospyros kaki by intestinal bacteria

Sheng-hai Zhang,Ying-zi Wang,Fan-yun Meng,You-lin Li,Cai-xia Li,Fei-peng Duan,Qing Wang,Xiu-ting Zhang,Chun-ni Zhang 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.5

Flavonoid glycosides are metabolized byintestinal bacteria, giving rise to a wide range of phenolicacids that may exert systemic effects in the body. Themicrobial metabolism of flavonoids extracted from theleaves of Diospyros kaki (FLDK) by intestinal bacteria wasinvestigated in vitro. High-performance liquid chromatography/linear trap quadrupole orbitrap mass spectrometrywas performed to analyze the metabolites of flavonoidsin vivo using Xcalibur2.1 software. The results showed thatthe levels of flavonoid glycosides and flavonoid aglyconesdecreased rapidly in the process of microbial metabolismby intestinal bacteria in vitro, and the metabolic rate maybe related to the concentration of intestinal bacteria in theculture solution. In vivo metabolites of FLDK weredetected in rat plasma and urine after oral administration ofFLDK. Eight flavonoids were identified in the urine, andthree were identified in the plasma; however, flavonoidaglycones were not found in the plasma.

Preparation and Characterization of Core/Shell-type Ag/Chitosan Nanoparticles with Antibacterial Activity

Lin, Yue,Jing, Wang,Kang, Pan,Xiaoming, Zhang,Zhouping, Wang,Wenshui, Xia Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.4

Making use of chitosan (CS) and ethylenediaminetetraacetic acid (EDTA) as a reaction system, CS-EDTA nanoparticles were synthesized through a facile counterion complex coacervation method. $Ag^+$ could enter porous CS nanoparticles synthesized with this method, allowing Ag nanoparticles within chitosan nanoparticles were synthesized by reducing silver nitrate with chitosan. Because of the noncovalent interaction between CS and EDTA, the EDTA could be easily removed via dialysis against water, and pure core/shell-type Ag/CS nanoparticles could be obtained. The nanoparticles showed higher antibacterial activity toward E. coli than the active precursor Ag nanoparticles and CS.

XPG is Predictive Gene of Clinical Outcome in Advanced Non-small-cell Lung Cancer with Platinum Drug Therapy

Zhang, Tian,Sun, Jing,Lv, Min,Zhang, Lin,Wang, Xia,Ren, Ji-Chen,Wang, Bin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Polymorphisms in XPG are considered to contribute to the clinical outcome of patients receiving platinum drug chemotherapy. We aimed to investigate the role of five potential SNPs of XPG gene on the response to platinum-based chemotherapy in advanced Chinese NSCLC patients. A total of 451 patients with newly diagnosed and histopathologically confirmed primary NSCLC were consecutively collected. XPG rs2296147, rs4150261, rs17655, rs1047768 and rs2094258 were genotyped by the Taqman real-time polymerase chain reaction (PCR). In our study, we found patients carrying rs1057768 TT genotype had a significantly lower treatment response when compared with the CC genotype (OR=0.38, 95% CI=0.18-0.78). Patients carrying rs1047768 TT genotype showed a significantly short median PFS (11.2 months) and OS (13.6 months) than CC genotype, and the hazard ratios (HR) for PFS and OS were 2.06 (1.01-4.50) and 2.29 (1.21-2.49), respectively. Moreover, we found a significant decreased risk of death from NSCLC among patients carrying the rs2296147 TT genotype when compared with the CC genotype, the HR (95% CI) for OS being 0.50 (0.27-0.95). In conclusion, our study found that polymorphisms in rs1047768 C/T and rs2296147 C/T are associated with response to platinum-based chemotherapy in advanced NSCLC, and XPG polymorphisms could be predictive of prognosis.

Erratum to: The treatment effect of novel hGHRH homodimer to male infertility hamster

Zhang, Xu-Dong,Guo, Xiao-Yuan,Tang, Jing-Xuan,Yue, Lin-Na,Zhang, Juan-Hui,Liu, Tao,Dong, Yu-Xia,Tang, Song-Shan The Korean Society of Pharmacology 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.1

Fabrication of Unique and Green Tin(IV) Sulfide/Moss Photocatalyst for Highly Effective Photocatalytic Reduction of Cr(VI)

Xia Zhang,Yumin Wang,Chao Hou,Xiaohui Lin,Hongli Jiang,Zhenhua Wei 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.3

We report a simple and green method for fabricating SnS2 modified moss composites for removal of heavy metal (Cr(VI)) considering the fact that the issue of wastewater caused by the discharge of heavy metal is becoming increasingly serious. More importantly, this kind of functional materials that are capable of removing effectively Cr(VI) from wastewater for water purification is quite few on the market. Hence, in the paper, such SnS2 decorated moss photocatalysts for removal of heavy metal (Cr(VI)) were designed and prepared via a simple and green method: chemical bath reaction. The as-designed materials (C@SnS2) integrate the independent properties of moss (with excellent performance in absorption of heavy metal) and SnS2 (with good photocatalytic reduction of Cr(VI) under visible light irradiation), showing a faster photocatalytic rate than those independent components. The experiment results indicate that more than 98.5% of Cr(VI) was reduced under visible light irradiation for 70 min, while only 18.2% and 84.4% of Cr(VI) were removed in the presence of C-moss and pure SnS2 at the same condition, respectively. Furthermore, the as-designed C@SnS2 has good recyclability in the removal of Cr(VI). We believe such simple and green method to prepare photocatalyst for wastewater treatment with excellent performance will provide a good platform to design new photocatalytic materials for water purification.

Synthesis of Ag/TiO₂ Core/Shell Nanoparticles with Antibacterial Properties

Lin, Yue,Qiqiang, Wang,Xiaoming, Zhang,Zhouping, Wang,Wenshui, Xia,Yuming, Dong Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.8

Monodispersed Ag/$TiO_2$ core/shell nanoparticles were synthesized in solution via colloid-seeded deposition process using Ag nanoparticles as colloid seeds and $Ti(SO_4)_2$ as Ti-source respectively. Silver nitrate was reduced to Ag nanoparticles with $N_2H_4{\cdot}H_2O$ in the presence of CTAB as stabilizing agent. The titania sols hydrolyzed by the $Ti(SO_4)_2$ solution deposited on the surface of Ag nanoparticles to form the Ag/$TiO_2$ core/shell nanoparticles. Inductively coupled plasma atomic emission spectrometry (ICP-AES) showed low amount of Ag ion leaching from the Ag/$TiO_2$ core/shell nanoparticles. The Ag/$TiO_2$ core/shell nanoparticles indicated excellent antibacterial effects against Escherichia coli and maintained long-term antibacterial property.

Impact of wilting and additives on fermentation quality and carbohydrate composition of mulberry silage

Zhang, Ying Chao,Wang, Xue Kai,Li, Dong Xia,Lin, Yan Li,Yang, Fu Yu,Ni, Kui Kui Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.2

Objective: The objective of this study was to investigate the effects of wilting and additives on the fermentation quality, structural and non-structural carbohydrate composition of mulberry silages. Methods: The selected lactic acid bacteria strains Lactobacillus plantarum 'LC279063' (L1), commercial inoculant Gaofuji (GF), and Trichoderma viride cellulase (CE) were used as additives for silage preparation. Silage treatments were designed as control (CK), L1, GF, or CE under three wilting rates, that is wilting for 0, 2, or 4 hours (h). After ensiling for 30 days, the silages were analyzed for the chemical and fermentation characteristics. Results: The results showed that wilting had superior effects on increasing the non-structural carbohydrate concentration and degrading the structural carbohydrate. After ensiling for 30 days, L1 generally had a higher fermentation quality than other treatments, indicated by the lower pH value, acetic acid, propionic acid and ammonia nitrogen (NH₃-N) content, and the higher lactic acid, water soluble carbohydrate, glucose, galactose, sucrose, and cellobiose concentration (p<0.05) at any wilting rate. Wilting could increase the ratio of lactic acid/acetic acid and decrease the content of NH₃-N. Conclusion: The results confirmed that wilting degraded the structural carbohydrate and increased the non-structural carbohydrate; and L1 exhibited better properties in improving fermentation quality and maintaining a high non-structural carbohydrates composition compared with the other treatments.

The treatment effect of novel hGHRH homodimer to male infertility hamster

Zhang, Xu-Dong,Guo, Xiao-Yuan,Tang, Jing-Xuan,Yue, Lin-Na,Zhang, Juan-Hui,Liu, Tao,Dong, Yu-Xia,Tang, Song-Shan The Korean Society of Pharmacology 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.6

Extra-hypothalamic growth hormone-releasing hormone (GHRH) plays an important role in reproduction. To study the treatment effect of Grin (a novel hGHRH homodimer), the infertility models of 85 male Chinese hamsters were established by intraperitoneally injecting 20 mg/kg of cyclophosphamide once in a week for 5 weeks and the treatment with Grin or human menopausal gonadotropin (hMG) as positive control was evaluated by performing a 3-week mating experiment. 2-8 mg/kg of Grin and 200 U/kg of hMG showed similar effect and different pathological characteristics. Compared to the single cyclophosphamide group (0%), the pregnancy rates (H-, M-, L-Grin 26.7, 30.8, 31.3%, and hMG 31.3%) showed significant difference, but there was no difference between the hMG and Grin groups. The single cyclophosphamide group presented loose tubules with pathologic vacuoles and significant TUNEL positive cells. Grin induced less weight of body or testis, compactly aligned tubules with little intra-lumens, whereas hMG caused more weight of body or testis, enlarging tubules with annular clearance. Grin presented a dose-dependent manner or cell differentiation-dependentincrease in testicular GHRH receptor, and did not impact the levels of blood and testicular GH, testosterone. Grin promotes fertility by proliferating and differentiating primitive cells through up-regulating testicular GHRH receptor without triggering GH secretion, which might solve the etiology of oligoasthenozoospermia.

The Application of Decellularized Adipose Tissue Promotes Wound Healing

Xia Zenan,Guo Xiao,Yu Nanze,Zeng Ang,Si Loubin,Long Fei,Zhang Wenchao,Wang Xiaojun,Zhu Lin,Liu Zhifei 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.6

Background: Due to adipose-derived stem cells (ASCs) being easy to obtain, their rapid proliferation rate, and their multidirectional differentiation capabilities, they have been widely used in the field of regenerative medicine. With the progress of decellularized adipose tissue (DAT) and adipose tissue engineering research, the role of DAT in promoting angiogenesis has gradually been emphasized. Methods: We examined the biological characteristics and biosafety of DAT and evaluated the stem cell maintenance ability and promotion of growth factor secretion through conducting in vitro and in vivo studies. Results The tested ASCs showed high rat:es of proliferation and adhered well to DAT. The expression levels of essential genes for cell stem maintenance, including OCT4, SOX2, and Nanog were low at 2–24 h and much higher at 48 and 96 h. The Adipogenic expression level of markers for ASCs proliferation including PPARγ, C/EPBα, and LPL increased from 2 to 96 h. Co-culture of ASCs and DAT increased the secretion of local growth factors, such as VEGF, PDGF-bb, bFGF, HGF, EGF, and FDGF-bb, and secretion gradually increased from 0 to 48 h. A model of full-thickness skin defects on the back of nude mice was established, and the co-culture of ASCs and DAT showed the best in vivo treatment effect. Conclusion: The application of DAT promotes wound healing, and DAT combined with ASCs may be a promising material in adipose tissue engineering and regenerative medicine.

Experimental study on the combined effects of obstacles and local water mist on gas explosion for maritime safety

Zhang Bin,Li Zhuoran,Xia Yuanchen,Shi Jihao,Zhang Jinnan,Wang Boqiao,Yu Jiahang,Qu Yanxu,Chen Li,Lin Yejin,Wu Wanqing 대한조선학회 2022 International Journal of Naval Architecture and Oc Vol.14 No.1

A variety of experimental configurations including different water mist obstacles, are used to investigate the combined effects of obstacles and water mist upon the gas explosion. The results demonstrate the 8 mm water mist can significantly inhibit the deflagration, while both 45 mm and 80 mm water mists exhibit the opposite effect under all the locally distributed positions of water spray nozzle without obstacles inside the vessel. When considering the obstacles, the 45 mm water mist starts to mitigate the deflagration and its mitigation effect is more significant than that induced by the 8 mm water mist. What's more, the 80 mm water mist can slow down the flame propagation speed while it would still lead to the gas explosion. Additionally, there remains unchanged about the effect of locally spraying 8 mm water mist upon deflagration at the initial stage of flame development as varying the position of obstacles, while the effects of mitigating deflagration by 45 mm and 80 mm water mist are decreased. The results will make contributions to design the arrangement of equipment and water mist configuration on the offshore platform or NG-fueled ship's engine room so as to mitigate the gas explosion accident.