연잎, 연자육, 연자방 에탄올 추출물의 항염증 활성

이은주(Eun-Joo Lee),서유미(Yu-Mi Seo),김용현(Yong-Hyun Kim),정정욱(Chungwook Chung),성화정(Hwa-Jung Sung),손호용(Ho-Yong Sohn),박종이(Jong-Yi Park),김종식(Jong-Sik Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.4

연은 아시아 국가에서 음식과 약재로 널리 사용되는 재료이다. 본 연구에서는 연의 잎(leaf, NL), 자육(seed, NS), 자방(seedpod, NSP)으로부터 에탄올 추출물을 제조하고 이들의 항염증 활성과 작용기전을 규명하였다. 이들의 항염증 활성을 연구하기 위하여 LPS로 자극된 RAW 264.7 세포에서 nitric oxide (NO) 생산을 측정하였다. NL, NS, NSP는 세포 생존율에 영향을 주지 않으면서, 농도의존적으로 NO의 생산을 현저하게 저해하였으며, iNOS 및 COX-2와 같은 pro-inflammatory 중재자들의 단백질 발현을 감소시켰다. 또한, NL, NS, NSP는 MAPKs 단백질의 인신화를 감소시키고 NF-κB p65의 핵으로의 이동을 저해함으로써, 세 추출물에 의한 항염증 활성은 MAPKs 경로와 NF-κB 경로를 조절함으로써 이루어짐을 제시한다. 게다가, ROS의 생성이 세 추출물에 의해서 모두 저해되었으며, HO-1의 발현과 HO-1의 전사조절인자인 Nrf2의 핵으로의 이동이 증가되었다. 결론적으로, 이러한 연구결과는 연의 다양한 부위의 추출물인 NL, NS 그리고 NSP는 항염증 활성을 가지고 있으며, MAPKs, NF-κB, Nrf2/HO-1 등 다양한 신호경로를 통해 조절할 수 있음을 제시한다. Nelumbo nucifera, also known as sacred lotus, has mainly been used as a food throughout the Asian countries. In the present study, we prepared ethanol extracts from leaf (NL), seed (NS), and seedpod (NSP) of Nelumbo nucifera and investigated their anti-inflammatory activities in mouse macrophage RAW 264.7 cells. To evaluate the anti-inflammatory activities of NL, NS, and NSP, nitric oxide (NO) production was measured in LPS-stimulated RAW 264.7 cells. NL, NS, and NSP significantly reduced NO production in a dose-dependent manner without affecting cell viabilities. NL, NS, and NSP dramatically decreased the protein expression of pro-inflammatory genes such as iNOS and COX-2. NL, NS, and NSP also suppressed phosphorylation of MAPKs and the nuclear translocation of NF-κB p65 indicating they have their anti-inflammatory activities via regulating mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) pathways. In addition, we analyzed the production of reactive oxygen species (ROS) by the treatment of NL, NS, and NSP. All extracts reduced ROS production in a dose-dependent manner. And also, they increased heme oxygenase-1 (HO-1) protein expression and the nuclear translocation of nuclear respiratory factor 2 (Nrf2). In conclusion, our results suggest that Nelumbo nucifera has its anti-inflammatory activity via regulating MAPKs, NF-κB, and Nrf2/HO-1 pathways.

건조 상추 에탄올 추출물의 항염증 활성

이은주(Eun-Joo Lee),서유미(Yu-Mi Seo),김용현(Yong-Hyun Kim),정정욱(Chungwook Chung),성화정(Hwa-Jung Sung),손호용(Ho-Yong Sohn),박종이(Jong-Yi Park),김종식(Jong-Sik Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.3

상추는 가장 선호하는 녹색 채소 중 하나이다. 상추는 폴리페놀성 화합물을 비롯한 다양한 성분을 함유하고 있으며, 항균, 항산화, 항염증 등의 생리활성을 가지고 있는 것으로 알려져 있다. 본 연구에서는 건조상추의 에탄올 추출물(DLE)을 제조하고 이들의 항염증 활성을 연구하였다. DLE의 항염증 활성을 측정하기 위하여 LPS로 활성화된 마우스 대식세포 RAW 264.7 세포주에서 nitric oxide (NO) 생성을 측정하였다. DLE는 세포주의 생존에는 영향을 미치지 않으면서 NO 생산을 현저하게 저해하였다. DLE에 의해 염증 유전자인 iNOS와 COX-2의 유전자와 단백질의 발현이 모두 감소하였으며, 6개의 염증관련 cytokine 유전자(IL-1α, IL-1β, IL-1F6, TNF-α, CSF2, 그리고 CXCL10)의 발현이 모두 감소하였다. 또한, DLE의 처리는 MAPKs 경로의 인산화를 모두 저해하였으며, NF-κB p65의 핵으로의 이동을 저해하였다. 이러한 결과는 DLE의 항염증 활성은 MAPKs 경로와 NF-κB 경로를 조절함으로써 이루어짐을 시사한다. 또한, DLE는 농도의존적으로 reactive oxygen species (ROS)의 생산을 저해하였으며, hemeoxygenase-1 (HO-1) 단백질의 발현을 증가시켰으며, HO-1의 전사조절인자인 Nrf2의 핵으로의 이동을 증가시켰다. 결론적으로, 이러한 연구결과는 DLE가 염증관련 유전자의 발현을 감소시키며, MAPKs, NF-κB, 그리고 Nrf2/HO-1 등 다양한 경로를 조절함으로써 항염증 활성을 가지는 것을 제시한다. Lettuce (Lactuca sativa L.) is one of the most popular green leafy vegetables, and it contains various beneficial components including polyphenolic compounds and has been known to possess various biological functions such as anti-microbial, anti-oxidative, and anti-inflammatory activities. In the present study, we prepared ethanol extract of dried lettuce (DLE) and investigated its anti-inflammatory activity. To evaluate the anti-inflammatory activity of DLE, nitric oxide (NO) production was measured in LPS-activated mouse macrophage RAW 264.7 cells. DLE significantly suppressed NO production in these cells without affecting cell viabilities while resveratrol was used as a positive control. DLE dramatically decreased the expression of pro-inflammatory genes such as iNOS and COX-2 at the mRNA and protein levels and reduced the expression of several cytokines including IL-1α, IL-1β, IL-1F6, TNF-α, CSF2 and CXCL10. In addition, DLE suppressed phosphorylation of MAPKs and the nuclear translocation of NF-κB p65 indicating DLE shows its anti-inflammatory activity via regulating MAPKs pathway and NF-κB pathways. And also, DLE reduced the production of reactive oxygen species in a dose-dependent manner. DLE increased HO-1 protein expression, and also increased the nuclear translocation of Nrf2. Overall, our results suggest that lettuce down-regulate various pro-inflammatory genes and have its anti-inflammatory activity via regulating MAPKs, NF-κB, and Nrf2/HO-1 pathways.

사례기반 추론을 이용한 공정설계

서윤호,고범석,김용태 울산대학교 2002 공학연구논문집 Vol.33 No.1

This paper introduces a method to automate process design using Case Based Reasoning technique. Particularly, the function-behavior-structure model is used to represent a process plan as a case. Specifically, a case retrieval algorithm to use function and behavior, case adaptation knowledge definition by using neural network technique, and a machine selection method are presented.

뇌졸중 후 반사성 교감신경성 위축증후군에서체표온도의 정량적 분석

신용일,양선호,서정환,김연희 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.3

상완골 근위부 분쇄 골절에서의 상완골 두 치환술

서중배,원중희,김용민,최의성,이호승,홍윤철 대한견.주관절학회 2000 大韓肩.주關節學會誌 Vol.3 No.2

협동학습이 자연과 학업성취 및 과학태도에 미치는 효과

서승조,백남권,조태호,김용진 晋州敎育大學校 科學敎育硏究所 1999 科學敎育硏究 Vol.25 No.-

The purpose of this study is to manifest the effect of STAD cooperative learning which has not yet been explained in cooperative learning of an elementary school, on the academic achievement in science and science related attitudes. Therefore, in order to achieve that purpose, I established the following issues of study. First: What effect does STAD cooperative learning have on the elementary school students' academic achievement in science? Second: What effect does STAD cooperative learning have on the elementary school students' science related attitudes? The results of the study are as follows. First, while it was proven that the effect of STAD cooperative learning on academic achievement in science had no significant difference in pre·post achievement levels for both the experimental group and the control group, the experimental group showed larger changes in the mean value than control group; it can be inferred that classes to which cooperative learning is applied is more effective than traditional science classes. Second, in regard with the effect of STAD cooperative learnign on science related attitudes, while there was a significant difference between the experimental group and control group in pre examination, there was no significant difference, which means STAD cooperative learning does have effect on science related attitudes. In conclusion, the results of this study manifest that STAD cooperative learning is effective for science related attitudes in elementary school science classes, and assume that it can be effective for academic achievement.

자동차차체 및 부품제조업 산업장의 작업환경실태에 관한 조사 연구

서준호,문덕환,김정호,이채관,황용식,손병철,김대환,이창희,김휘동,이채언 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.5

Objectives: For the purpose of preparing the fundamental data and control the working environment and assessing the status of working environmental in manufacturing industry of bodies for motor car and of parts. Methods and Materials: Accessories for motor car and its engines the auther measured the noise level, concentration of dust, heavymetals, organic solvents, chemicals to 99 industries(24 working processes) form Feb. 2000 to Oec. 2000. Results: The results were as follows : 1. Mean noise level cutting, grinding, pressing, foundries and shot-blast was exceeded to threshold limit value(TLV) of noise. 2. Mean concentration of total dust was not exceeded to TLV. 3. Mean concentration of Mn in welding process and Pb in drying, welding, fabricating, rolling was exceeded to TLV. 4. Mean concentration for chemicals and organic solvents were not exceeded to TLV. 5. Mean concentration of dust and heavymetals were statistical significant difference between with local ventilation system and without local ventilation system. Conclusion: Above results, author suggest to prepare more passively control to working environment where exceeded the TLV.

전류평형 제어기를 부가한 IGBT 모듈의 모델링

서영수,송호빈 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

Due to tolerances of semiconductor, gate-drive and mechanical parameters the total load current is not shared equally between paralleled IGBT power modules. In this paper, a closed loop control is presented to balance the current in a gate-drive circuits. The theoretical assumptions have been verified through SIMULATION with PSPICE.

유도전동기 운전을 위한 외란상쇄 상태관측기 설계

서영수,송호빈,김용주 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

Abstract - This paper described a robust control of an induction motor using a disturbance cancellation observer of a feedforward control with Matlab simulink. The speed response of conventional PI controller characteristics is affected by variations of load torque disturbance. In the proposed system, the speed control characteristics using a feedforward control isn't affected by a load torque disturbance.

원형탈모증의 임상양상 및 기술역학적 연구

이용호,하헌용,이동초,김미경,서필승,김남송 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

본 연구는 2004년 1월부터 2008년 10월까지 원광대학교 병원 피부과에 내원한 원형 탈모증 환자 126명중 치료 결과를 확인 할 수 있었던 95명을 대상으로 인구학적 특성 및 치료 방법에 따른 치료 효과의 차이를 알아보고자 빈도 분석 및 교차분석을 통하여 유의성을 검정하였다. 탈모반면적은 성별에서 남자와 여자가 각각 26-50%일 때 53.5%, 71.4%로 가장 많았으나 유의한 차이는 없었고, 연령별로는 20대 이하와 30대, 40대 이상이 각각 26-50%일 때 54.1%, 51.9%, 58.6%로 가장 많았으나 유의한 차이는 없었다. 치료방법은 성별에서 남자와 여자 각각 부신피질호르몬 국소방법을 94.3%, 85.7%로 가장 많았으나 유의한 차이는 없었고, 연령별로는 20대 이하, 30대, 40대 이상이 각각 스테로이드 국소 주사요법을 89.7%, 100%, 93.1%로 가장 많았으나 유의한 차이는 없었다. 치료효과는 성별에서 남자와 여자 각각 완치하였다가 64.8%, 71.4%로 가장 많았으나 유의한 차이가 없었고, 연령별로는 20대 이하와 30대의 경우 완치되는 경우가 각각 71.8%, 85.2%로 가장 많았고, 40대 이상은 부분반응 하는 경우가 58.6%로 가장 많았으며 통계적으로 유의한 차이를 보였다(p<0.05) 치료방법에 따른 치료효과에는 유의한 차이가 있었다(p=0.003). 스테로이드 pulse therapy를 사용한 경우 완치가 100%, DPCP 요법은 완치가 77.8%, minoxidil 국소요법은 완치가 73.8%, 스테로이드 pulse therapy의 경우 완치가 100%로 가장 많았다. Background: We embarked on this research in order to gain fundamental information and knowledge on the key differences in the effectiveness of various treatments and factors, such as key clinical conditions and demographic traits through the descriptive research-based procedures, on patients with alopecia areata. Methods: Based on the comprehensive data of 95 patients with verifiable treatment records out of 126 alopecia areata patients who have visited the department of dermatology of Wonkwang University Hospital from January 2004 to October 2008, We examined the significance tests through the analysis of frequency and range of the differences in the effectiveness of various treatments using demographic traits and medical treatments. Results: In terms of the treatment effectiveness, 64.8% of the male patients and 71.4% of the female patients showed the full recovery, with virtually no significant clinical data difference. In terms of the age group variations, 71.8% of the patients in 20's and under and 85.2% of the patients in 30's showed the full recovery, and 58.6% of 40's showed only partial recovery, rendering this difference quite significant.(p<0.05). There was a noticeable difference in effectiveness of various treatments.(p<0.01). Through the steroid pulse therapy, 100% of the patients fully recovered, 77.8% through the DPCP(Diphencyprone) method, 73.8% through the minoxidil application treatment, 65.2% through the steroid injection treatment, and finally 33.3% of the steroid application treatment fully recovering from the hair loss. Conclusion: In terms of the effectiveness, the steroid pulse therapy were most dominant and clear treatment, followed by DPCP treatment, the minoxidil application treatment, the steroid injection treatment, and the steroid application treatment in this order. In terms of the distribution of the gender factor of the observed patients, the number of female patients was far smaller than that of male patients, and tremendous barriers and challenges lie in the quest to make a conclusive finding through the use of the raw data of hospital patients. As such, a variety of rigorous and dynamic clinical research endeavors and the large sample of patients are necessary and strongly recommended at this point.