약물의 폐 전달을 위한 염화 벤제토늄 기반 리포좀의 제조 및 평가

문용현, 유소열, 안지선, 이한솔, 김혜림, 이은주, 이재영 충남대학교 약학대학 의약품개발연구소 2021 藥學論文集 Vol.36 No.-

Benzethonium chloride-containing egg phosphatidylcholine-based liposomes (BC/EPC-LIPOs) were developed for lung-targeted drug delivery. BC/EPC-LIPOs were fabricated via thin-film hydration method with various BC-to-EPC molar ratios of 1:4 (F1), 1:1 (F2), and 4:1 (F3). Cyanine5.5 (Cy5.5) was loaded as a fluorescent probe for the biodistribution study during the film-forming step. BC/EPC-LIPOs were evaluated by using dynamic light scattering analysis, where the mean diameter and zeta-potential values of F1, F2, and F3 were observed to be 69.5 ± 3.1 nm and 50.06 ± 1.00 mV, 59.0 ± 2.3 nm and 51.30 ± 2.98 mV, and 694.2 ± 243.3 nm and 55.01 ± 3.16 mV, respectively. To evaluate the lung distribution efficiency of the developed liposomes, F1 and F2, which possess suitable particle sizes for the intravenous administration, were injected into the tail vein of ICR mice. At 6 h post-injection, the lung, liver, kidney, heart, spleen, and brain were dissected, and the radiant efficiency (RE) values of the or- gans were measured using the near-infrared fluorescence imaging method. The lung RE value of F2 was 1.69-fold higher than that of F1, indicating a more efficient lung targetability of F2. This phenomenon can be attributed to the higher content of BC in F2 than F1. Considering the lung-targetability, as well as the biocompatible natures of BC and EPC, the developed BC/EPC-LIPOs can be used as an efficient lung-targeted drug delivery system.

수생식물의 중금속 흡수능에 관한 연구

이종화,함용규,박종안 한국환경독성학회 1996 환경독성보건학회지 Vol.11 No.2

In this study, we investgated the uptake capacity of several water plants for heavy metals (lead and cadmium) in soil of rivers where are adjacent to a industrial complex in Chun-An city and in A-San city. We also examined the deposition pattern of heavy metal in plants. The results are as follows: 1. The soil of river in Chun-An city was polluted more serious than that of A-San city. In Chun-An city, mean values of lead and cadmium contents in soil were 26.224±28.037㎍/g, and 0.854±1.127㎍/g, respectively. 2. Water plants examined in this study were Sium suave KITAGAWA, Persicaria thunbergii H. GROSS, Phragmites japonica STEUD, Echinochloa crus-galli var. frumentacea WIGHT and Persicaria hydropiper SPACH. Both metal contents of several water plants distributed in Chun-An city were higher than those in A-San city. In these plants, Sium suave showed the highest uptake capacity for lead and cadmium. The mean values of lead and cadmium contents in Sium suave were 40.957 ±29.577 ㎍/g and 1.930±1.076 ㎍/g, respectively. Persicaria thunbergii also showed a relatively high uptake capacity for both metal. 3. Correlation between metal contents in soil and water plants was high. In both cases of Sium suave and Persicaria thunbergii correlation coefficients were 0.605 and 0.549, respectively. 4. We analyzed lead and cadmium contents in root, stem and leaf of several water plants. Both metals were mostly deposited in root. Much of both metals were also deposited in leaf. From the results, we suggest that Sium suave KITAGAWA and Persicaria thunbergii H. GROSS can be used to reduce heavy metals from industrial waste water.

상악동을 침범하는 역위성 유두종의 증례보고

지용화,최보람,허경회,안창현,이삼선 대한구강악안면방사선학회 2009 Imaging Science in Dentistry Vol.39 No.2

The present study reports a case of inverted papilloma of the nasal cavity and infiltrating into the maxillary sinus. Inverted papilloma is an uncommon and locally aggressive benign tumor of the sinonasal region. The patient, 51-year-old male, presented with unilateral nasal obstruction and periodic swelling on the palate without pain. Enhanced CT scan revealed a heterogeneously enhancing solid mass in the nasal cavity and infiltrating into the right maxillary sinus, as well as an incidental, secondarily infected residual cyst m the periapical area of the right maxillary canine. The sinonasal mass was revealed as an inverted papilloma on histopathologic examination.

Novel Methylation Biomarker for Non-invasive Diagnostics in Lung Cancer

오태정,( Chang Hun Lee2 ),( Min Ki Lee ),( Yeul Hong Kim ),( Sang Yull Lee ),( Hyo Sung Jeon ),( Shin Yup Lee ),( Seung Soo Yoo ),( Jae Yong Park ),( Sung Whan An ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

To identify aberrantly hypermethylated DNA in lung cancer cells we established a genome-wide analysis for hypermethylation sites, namely Methyl DNA Isolation and Amplification (MeDIA) coupled-CpG microarray analysis. In the comprehensive methyaltion profiling analysis between human lung cancer, A549 cells and normal NHBE cells, we observed that several clusters of genes show a significant level of aberrancy in CpG island methylation pattern in cancer cells compared to normal cells. We further identified PCDHGA12 gene as a new marker of non-invasive diagnostics for lung cancer based on followings. 1) Transcription of PCDHGA12 gene is reactivated after treatment of A549 cells with demethylating agent. 2) Bisulfide clonal-sequencing reveals that CpG island of PCDHGA12 shows a distinctive differential methylation between two cell lines. 3) Pyrosequencing-based quantitative methylation assay for such region in tumor and non-tumorous tissues from lung cancer patients shows aberrant hypermethylation in 37 (92%) of the 40 tumor tissues. In clinical validation by pyrosequencingin induced-sputum of lung cancer patients (n=87) and healthy controls (n=51), we observed aberrant hypermethylation incident at significantly elevated level in samples derived from lung cancer patients. According to the optimal threshold calculated by ROC curve analysis, sensitivity and specificity of PCDHGA12 was 86.2% and 82.4%, respectively. PCDHGA12 methylation status could be a potential methylation biomarker alone or combined with others for the screen and the detection of relapse of lung cancer.

Thermal Expansion Behavior of Thermally Expandable Microspheres Prepared by Suspension Polymerization Using P(AN-MMA-MAA) Core/Shell

Hyun Tae Kim,Ashok Kumar Jaladi,Yong Jin Lee,Duk Keun An 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.2

Thermally expandable microspheres (TEMs) have widespread industrial applications. In this study, high heat resistant and largely expandable TEMs have been synthesized by suspension polymerization. Microspheres were prepared with acrylonitrile (AN) as the main monomer. Initial thermomechanical analysis of TEMs containing AN and methyl acrylate (MA) showed good thermal expansion, leading to the investigation of the polymerization using various monomers at variable weight ratios. TEMs with co-polymer AN, methyl methacrylate (MMA), and methacrylic acid (MAA) were investigated for the first time. They displayed wider heating range and larger expansion rate than the microspheres with MA monomer. The effect of varying amounts of monomer ratios on the thermal expansion behavior is discussed.

Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

중·고 하키지도자의 지도유형에 따른 선수만족과의 관계

안용덕,김종이,이승준,신명건 朝鮮大學校 스포츠科學硏究所 2007 스포츠科學硏究 Vol.18 No.-

선형파괴역학에 의한 Aluminum Foil과 종이의 파괴거동 해석

안득만,신용산,이현웅,옥영구 釜山大學校生産技術硏究所 1999 生産技術硏究所論文集 Vol.57 No.-

크랙이 내재된 시편을 크랙으로부터 멀리 떨어진 단면에 대해 균일한 크기의 응력과 변형량이 발생하도록 길이방향으로 당겨주는 장치를 이용하여, 선형 파괴 역학적 관점에서 알루미늄 호일과 종이의 파괴거동을 평가하였다. 상사형의 균열을 가진 파괴시편의 파괴하중을 차원해석으로 예측하였고, 실험을 통하여 예측된 하중과 실제하중을 비교, 검토하였다. The fracture behaviors of aluminum foils and sheet papers were analyzed on the basis of linear elastic fracture mechanics(LEFM). The fracture loads of the Al foil and paper were calculated by dimensional analysis. The actual fracture loads were measured using the simple tension equipment. The predicted fracture loads were compared with the experimental results, respectively.

Alloxan 유도 당뇨병상태에서 과산화지질생성에 미치는 Brazilin의 효과

안영수,길이룡,소동수,창동신,김진형,박광식,문창규 한국환경독성학회 1996 환경독성보건학회지 Vol.11 No.2

Brazilin was tested for its ability to inhibit alloxan induced lipidperoxidation. Lipid peroxide contents in liver, kidney and serum were measured by the TBA method. ICR mice receiving alloxan at a dose of 43mg/kg via the tail vein after a 24hrs starvation showed significantly increased lipid peroxide contents as compared to untreated control. Lipid peroxide contents in liver, kidney and serum of alloxan-induced diabetic mice were dose-dependently decreased by the treatment of brazilin at a dose of 10mg/kg, 50mg/kg, 100 mg/kg for 5 days.

교육대학 남학생들의 대학생활 적응과 만족도 연구

이관형,장용규,안현미 서울교육대학교 학생생활연구소 2000 學生生活硏究 Vol.26 No.-

본 연구는 교육대학 남학생들의 대학생활 적응과 대학생활 만족도를 살펴봄으로써 교육대학 남학생들의 대학생활에 관한 깊이있는 이해를 돕고 남학생들의 의미있고 원만한 대학생활을 위한 개선 방안을 모색하는데 그 목적을 두고 있다. 총 204명의 남학생들이 참여하였으며 대학생활적응척도(Student Adjustment to College Questionaire)가 사용되었고, 또한 남학생 소집단 대화 프로그램 참여자들을 대상으로 실시된 면접 자료들에 대하여는 질적 분석이 시도되었다. 주요 연구 결과는 다음과 같다. 학년별 대학생활 만족도의 차이를 살펴본 결과, 1학년 남학생 집단에 비하여 2학년과 4학년 남학생 집단이 유의미하게 높은 대학생활 만족도를 경험하는 것으로 나타났다. 또한, 군복무 경험은 대학생활 만족도에 영향을 미치는 중요한 요인으로 보이는데 학업적 적응, 사회적 적응, 개인-정서적 적응, 대학 만족도 등의 각 영역에서 군복무 경험이 있는 남학생 집단이 보다 높은 대학생활 만족도를 경험하는 것으로 나타났다. 반면에, 재수 경험이 있는 남학생들의 경우 대인관계 및 대학 생활 적응에 어려움을 더 많이 겪는 것으로 나타났다. 교육대학 남학생들의 거주형태를 알아본 결과 자택에 거주하는 학생은 51.5%로 나타났으며 그 외 자취나 고시원 등에서 생활하는 경우도 48.5%로 나타났다. 자취나 고시원 생활을 하는 남학생들은 자취 방이나 고시원의 좁고 밀폐된 생활공간이나 위생 등의 문제로 불편함이 많다고 호소하였으며 심리적으로도 불안정하고 위축된 생활을 하고 있는 것으로 나타났다. 남학생 전용 기숙사 시설의 설립 문제에 대해서는 설문 대상자의 86.6%가 '필요하다'는 응답을 보였다. 끝으로, 이 연구 결과들을 기초로 하여 교육대학 남학생들의 건강한 대학생활 영위를 효율적으로 돕기 위한 대안들을 제시하였다. Focused on the male groups of Seoul National University of Education, the present study explored the campus life adjustment and satisfaction index. The purpose of this study was to examine the degee of campus life adjustment and the causes of maladjustment of males and to consider some alternatives for the coping strategies. The data was collected from 204 male students with Student Adjustment to College Questionaire and through interview with structured group counseling. The results of this study are as follows. The majority of the senior males and sophomores expressed higher degree of satisfaction with regard to their campus life than the freshmen. The older students discharged from military service showed more satisfaction than the younger students in active service. But adjustment level was not higher in the students who had prepared themselves for this university entrance exams once more than other students. Forty eight percent, self-boarding students of males expressed many stresses on their university life in the actual conditions of residence. The majority of males strongly hoped that a university dormitory for males will be set up as soon as possible. Finally on the basis of the results, some discussions and suggestions could be given.