성인 급성 부고환염에서 Nested PCR에 의한 Chlamydia trachomatis의 검출

정원일,우승효,이형래,이상철,김용태,김원재 충북대학교 의학연구소 2000 忠北醫大學術誌 Vol.10 No.2

연구목적 : 성교가능 성인남자의 급성 부고환염에서 Chlamydia trachomatis가 차지하는 빈도를 Nested PCR을 통해 검출하여 보고자 하였다. 대상 및 방법 : 1996년 9월부터 1999년 2월까지 본 충북대학교 병원 비뇨기과 외래 및 응급실을 내원하여 급성 부고환염으로 진단된 환자 중 성교가능 성인남자 28명을 대상으로 하여 환자로부터 얻은 뇨검체로 일반 미생물 배양검사, 그람염색검사 및 Chlamydia PCR을 시행하였다. 결과 : 급성 부고환염으로 진단된 28명의 평균 연령은 30.9±12.4세 였으며 총 28명중 17명 (60.7%)에서 Nested PCR 검사에 양성으로 나타났으며 연령별로는 20대가 10명중 7명(70%)으로 가장 높은 양성율을 보였다. 그람염색도말 결과 28명중에서 6명(21%)이 비임균성 요도염에 양성을 나타냈으며 또한 일반 미생물 배양 검사 결과 2명에서 균이 자랐는데, 각각 S. epididermidis와 E. coli였다. 결론 : PCR을 통한 Chlamydia trachomatis의 검출이 지금까지 행해진 다른 검사 방법들에 비해 월등하게 민감도가 높으며 성접촉이 가능한 연령의 성인 남자가 급성 부고환염 증세로 내원시 기본적으로 PCR을 통한 Chlamydia trachomatis 검출 검사를 시행하여 적절한 치료가 이루어지는 것이 중요할 것이다. Purpose : We studied the incidence of Chlamydial epididymitis in sexually active male with acute epididymitis by the Nested polymerase chain reaction( PCR ). Materials and Methods: Urine specimens, collected from 28 patients that was 30.9±12.4 years old and presented with acute epididymitis, were tested for the presence of Chlamydia trachomatis by the Nested PCR and for other bacteria by the urine gram stain and culture. Results: Out of total 28 patients, 17 patients(60.7% ) showed positive for Chlamydia trachomatis by the Nested PCR. In thirtieth decade, 7 of 10 patients(70%) showed positive, revealed the highest incidence. 6 patients(21%) showed positive for other bacteria by the urine culture. Conclusion: Detection rate of Chlamydia trachomatis by the Nested PCR was higher than other current diagnostic technique. For the high incidence of Chlamydial epididymitis, all men with acute epididymitis should undergo Chlamydia trachomatis evaluation for adequate treatement of this infection.

해양미생물로부터 카로테노이드 색소의 생산을 위한 최적조건

정영기,박정욱,주우홍,류병호,정명주,김태수 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1

The optimal medium composition for the production of carotenoid pigment from Haloarcular sp. EH-1 as a dietary for fishes were 1.0% sucrose, 1.0% yeast extract, 25% sodium chloride, 0.3% sodium citrate, 0.2% potassium chloride, 2.0% magnesium sulphate, 0.002% ferric sulphate(pH7.0). The incubation temperate, aeration rate and agitation speed were 40℃, 100ml medium/500ml vol. shaking flask, and 180 rpm/min. The carotenoid pigment production was highest after 5 days of incubation with the light.

全州川의 水質汚濁에 관한 調査硏究

鄭遇喆,金煥起,李容源 全北大學校 附設 都市및環境硏究所 1987 都市 및 環境硏究 Vol.2 No.-

This paper analytically compared the water quality data with the effluent flow rate of the pollutent, in Chenju-cheon, during the past fifteen years. 1. The main sorces of pollutron were revealed as the domestic sewage and the industrial wastewater. 2. The yearly average of pollutant concentration has been decreasing but MPN has been incresing for the conversion of flush toilet. 3. ABS concentration has been decresing for the use of soft detergent. 4. The effluent flow rate of discharge was under the control of the geological and topographical property. 5. The discharge rate of BOD and SS was 29% and 6.5% at Hanbukru, 6% and 21% at Dukjinbo, 89% and 69% at Iseongbo.

Homomorphic Encryptions at a Long Distance Through Quantum Key Distribution Protocols

JEONG*, Kabgyun,KIM, Yong Jin,YOON, Jong Beom,KIM, Geon Woo,CHO, Gueha,KIM, Suntaek Korean Physical Society 2019 새물리 Vol.69 No.2

식품유해세균에 대한 식초의 항균효과

우승미,장세영,김옥미,윤광섭,정용진 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2004 한국식품저장유통학회지 Vol.11 No.1

본 연구에서는 시판되고 있는 식초를 이용하여 6종의 식품 유해세균에 대한 항균력을 비교하였다. 그 결과, 감식초와 현미식초는 합성식초에 비해 S. aureus, M. luteus, S. enteritidis 및 E. coli에서 항균 활성이 높게 나타났으며, 현미식초가 감식초에 비해 식품유해세균에 대한 항균활성이 비교적 더 높게 나타났다. 현미식초의 농도에 따른 항균활성을 조사한 결과 5종의 그람양성 세균 및 음성 세균 모두 10μm/mL 이상의 농도에서 생육이 거의 억제되었으나, V. parahaemolyticus는 15 μm/mL 농도에서 생육이 거의 정지되어, 현미식초는 식품 부패균과 대장균 생육억제에 우수한 것으로 나타났다. 식초처리에 의한 미생물의 형태 변화를 전자 현미경으로 관찰한 결과 S. aureus, E. coli 모두 세포가 팽윤되고 일부 세포벽이 완전히 파괴된 형태를 관찰할 수 있어 식초처리로 인해 세균의 생육이 억제되는 것을 알 수 있었다. We investigated antimicrobial effects of commercial vinegar on the harmful food-born organisms. As a result, antimicrobial effects of brown rice vinegar showed stronger than persimmon and artificial vinegar. In 10μL/mL concentration of brown rice vinegar was completely inhibited about 5 strains except for V. parahaemolyticus, it was inhibited 15 μL/mL concentration. Therefore vinegars were effective for inhibition acitivity against foodborne organisms. S. aureus and E. coli treated with 25μL/mL concentration brown rice vinegar was observed by scanning electron micrographs(SEM). The cells were expanded and a part of cell wall was completely destructed by brown rice vinegar.

분산제어 구조를 가지는 TDX-10 전전자 교환기에서 프로세서간 통신 네트워크 관리

정연쾌,우용태 國立 昌原大學校 基礎科學硏究所 1993 基礎科學硏究所論文集 Vol.4 No.-

This paper presents the structure of Inter-Processor Communication(IPC) network implemented for TDX-10 which is the large-capacity digital swiching system with distributed control being developed by ETRI. It also describes management function of the IPC network to provide reliable internal network path.

Bacillus sp. YJ-63이 생산하는 항곰팡이 항생물질의 구조분석

정영기,신영준,정명주,주우홍,최재수 Institute of Genetic Engineering Changwon National 2002 Gene and Protein Vol.6 No.-

토양으로부터 분리한 Bacillus sp. YJ-63으로부터 항진균성 항생물질을 분리하여 그 구조를 분석한 결과는 다음과 같다. 항생물질은 7개의 α-amino acid와 1개의 β-amino acid가 결합한 환상구조를 가지는 iturin E 계에 속하며 특히, β-amino acid의 methylene 수가 기존의 iturin E 보다 더 많은 것으로 나타났다. Structural analysis was performed by the ^1H-NMR, ^13C-NMR, amino acid composition analysis and FAB-mass. The instrumental analysis represented that the potential antifungal antibiotic belonged to the iturin E group antibiotic, consisting of 7 α-amino acid residues and a collection of β-amino acid with aliphatic side chain. Compared to the iturin E group, notably, the potent antifungal antibiotic from bacillus sp. YJ-63 carried longer β-amino acid side chain. In conclusion, these findings identified a potential antibiotic, which contained a stable cyclopeptide structure with long β-amino acid side chain.

Genome-wide investigation of a Korean synthetic breed, Woori-Heukdon using the Illumina PorcineSNP60K BeadChip

Yong‑Min Kim,Ha‑Seung Seong,Jung‑Jae Lee,Da‑Hye Son,Jin‑Su Kim,Soo‑Jin Sa,Young‑Sin Kim,Tae‑Jeong Choi,Kyu‑Ho Cho,Joon‑Ki Hong,Jung‑Woo Choi,Eun‑Seok Cho 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Woori-Heukdon (KWH) is a Korean synthetic pig breed generated using Chookjin-Duroc (KCD), Chookjin-Chamdon (KCC), and their crossbreds. Currently, there is a severe lack of studies investigating the Korean breed populations including wild boars (KWB) throughout the genome. Objective: This study was performed to investigate the genetic characteristics of Korean pig populations at the genome-wide level. Methods: Using the SNP dataset derived from genotyped and downloaded datasets using the Illumina PorcineSNP60K BeadChip, we compared the genomes of 532 individuals derived from 23 pig breeds to assess the genetic diversity, inbreeding coefficient, genetic differentiation, and population structure. Results: KWB showed the lowest average expected heterozygosity (HE = 0.1904), while KWH showed the highest genetic diversity (HE = 0.02859) among Korean populations. We verified that the genetic composition of KWH, showing USD of 74.8% and KCC of 25.2% in ADMIXTURE analysis. In population structure analyses, KCC was consistently shown to be separated from other pig populations. In addition, we observed gene flow from Western pigs to a part of Chinese populations. Conclusion: This study showed that Korean native pigs, KCC have genetic differences in comparison with Chinese and Western pigs; despite some historical records and recent genetic studies, we could not find any clear evidence that KCC was significantly influenced by Chinese or Western breeds in this study. We also verified the theoretical genomic composition of KWH at the molecular level in structure analyses. To our knowledge, this is the first genomic study to investigate the genomic characteristics of KWH and KCC.

평면 구성 및 공간 분석을 위한 프로그램 개발

정상규,박우장,이요희,이훈 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

This paper aims at developing a program for the composition of plan and the analysis of space. This program is able to draw plans more easily and quickly, and to seek a proper design solution by analyzing the topology of space based on Space Syntax(Hillier & Hanson, 1984). The program includes the Algorithms united some theories such as Shape Grammar(Stiny & Mitchell, 1980). Dimensionless Representation, Space Syntax and so on. Therefore we exhibit developing tools for modifying, editing and analyzing architectural plans.

여성 요도증후군에서 Terazocin의 효과에 대한 연구

우승효,신관희,한광희,정원일,이형래,이상철,김용태,김원재 충북대학교 의학연구소 2000 忠北醫大學術誌 Vol.10 No.2

연구목적: 여성 요도증후군을 보이는 환자에서 교감신경차단제인 terazocin을 복용한 후 치료 효과에 대한 각 증상의 변화와 부작용을 알아보고자 하였다. 대상 및 방법: 1997년부터 1999년까지 충북대학교병원 비뇨기과에 내원한 35명의 여성 중 요검사 및 요배양 검사, 그리고 다른 이학적 검사에서 이상이 없는 요도증후군 환자를 대상으로 간질성방광염 증상점수(ICSS)를 terazocin (3mg/day for 3 months) 투여 전후에 조사하였고, 치료 후 3개월과 1년에 Likert scale 점수를 조사하였다. 결과: ICSS는 비치료군은 18.3±5.9점, 치료군은 16.5±6.8점으로 두군간의 차이는 없는 것으로 나타났다(p=0.52). 치료군은 치료 후 3개월에 시행한 ICSS가 9.9±7.8점으로 치료 전에 비해 현격히 증상이 호전되는 것으로 나타났다(p=0.0001). 특히 3개월에 시행한 Likert scale에서는 주간 및 야간빈뇨, 절박뇨, 배뇨통의 횟수 및 강도, 그리고 전체적인 증상정도에서 40-70% 가량 호전된 것으로 밝혀졌으며 이는 1년 후에도 증상의 호전이 지속되는 것으로 나타났다. 비치료군에서의 1년 후 Likert scale은 6명 중 2명이 전체적인 증상의 호전이 있는 것으로 나타났다. 결론: Terazocin은 환자의 약 70%가량에서 증상의 호전을 가져왔고, 비치료군에서도 환자의 33%가 저절로 증상이 좋아진 것으로 보아 Terazocin은 여성 요도증후군에서 분명히 효과가 있으나 증상이 심할 경우 보조적인 치료로 이용되는 것이 바람직할 것으로 사료된다. Purpose: We performed to assess the effects and complications of terazocin in women with chronic female urethral syndrome. Materials and Methods : Thirty-five women with chronic female urethral syndrome were studied from March 1997 to August 1999. The subjects exhibited normal findings without the pyuria and bacteriuria in urinalysis and urine culture and were invested the interstitial symptom score(ICSS). 29 subjects of them took a terazocin 1-4 mg/day for 3 months. They were reevaluated for the ICSS and Likert scale after 3 months and for Likert scale after 1 year. Results: The ICSS before treatment were showed 18.3±5.9 in the untreated patient group and 16.5±6.8 in the treated patient group, and no statistically difference between both patient groups(p=0.52). In the treated patient group, the ICSS after treatment were showed 9.9±7.8, significant difference between before and after treatment(p=0.0001). The Likert scale was showed at 3 months after treatment that frequency, nocturia, urgency, pain frequency, pain intensity, daily activities, and overall symptoms were improved in 65.5%, 55.2%, 24.1%, 41.4%, 41.4%, 70%, 70%, and 70% of 29 subjects, each other. Overall symptom score assessed at 1 year were showed that 3 subjects was aggravated. In untreated patient group, 2 of 6 was improved in overall symptom score. Conclusion: Terazocin was effective above 70% in the treated group, and symptoms were improved spontaneously in a third of the untreated group. So, we thought that terazocin would be useful as supportive treatment in period of severe symptoms.