Effect of Developmental Lead Exposure on the Expression of Hippocampal NMDA Receptor Subunit mRNA

Kim, Tae-Wan,Chung, In-Sung,Bae, Jae-Hoon,Shin, Dong-Hoon,Lee, Mi-Young,Kim, Joon-Sik 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

목적: in vivo 및 vitro에서 해마 신경세포의 발생단계별 NMDA 수용체 아단위 mRNA 발현에 대한 연 폭로 영향을 알아보고자 하였다. 방법: 흰쥐 해마 신경세포의 발생단계별 NMDA 수용체 NR2A, NR2B 아단위 mRNA 발현에 대한 연의 영향은 정상군과 연 폭로군의 출생 후 7일, 14일, 22일 흰쥐의 해마에서 in situ hybridization으로 mRNA 발현 정도를 densitometer로 측정하여 비교하였고, 연과 NMDA 의 세포독성은 해마 신경세포 일차배양 후 도립현미경을 이용한 형태학적인 관찰과 LDH 활성도를 이용하여 측정하였다. 결과: 연 과 NMDA 에 의한 세포독성에 대한 in vitro 실험에서 형태학적 소견과 LDH 활성도에서 해마 미성숙 신경세포와 성숙 신경세포사이의 차이가 있었으므로, 연과 NMDA 독성효과는 해마 신경세포의 발달 단계에 따라 차이가 있다. 정상군의 해마에서의 NR2A mRNA 발현은 출생 후 연령이 증가함에 따라 점진적으로 증가하였으나, NR2B mRNA 발현은 출생 후 연령이 증가함에 따라 점진적으로 증가하였으나 NR2B mRNA 발현은 연령의 증가에 따른 변화가 없었다. 연 폭로에 희한 NR2A mRNA 발현은 유의하게 감소하였으나(p<0.05), NR2B mRNA 발현은 변화가 나타나지 않았다. 만성적 연 폭로는 NR2A를 포함하는 NMDA 수용체를 감소시킬 수 있음을 알 수 있다. 결론: 연은 해마신경세포의 발생단계에서 NMDA 수용체 아단위 특히 NR2A mRNA 발현의 변화를 야기하여 시냅스 신호 전달에 영향을 나타냄을 알 수 있었다. Hippocampus

통일교육 방식과 내용에 따른 통일·평화의식의 변화

정용하,김태완 釜山大學校 韓國民族文化硏究所 2005 한국민족문화 Vol.26 No.-

We are analyzing the effects of how does college student's view of unification and peace changes by method and contents of unification education. We invited progressive persons who have unrestricted thoughts about N.Korea and took parts in actual business for exchange between South and North Korea. The contents and style of this lecture are different to ordinary unification education. we also analyzed difference between before and after the students who took the class about awareness toward unification and peace. The result of analysis is as follows. It showed that unification education is improved, effected much student's recognition of unification and peace. Especially, the seven questions were 95% or more, nine questions were 99% in level of significance except five questions that are reason of unification, changing direction of N. Korea, friendly state toward unification and withdrawal of US army from Korea. By the result, we assume that the unification education effected change of student's recognition toward unification and peace. But, we can't measure which one effected more between method and contents of education. In conclusion we assume that change in method and contents of unification education influenced recognition of students. And these changes will greatly effect those who are called 'The New Generation'

혈소판 풍부혈장의 임상적 응용

하정완,김수관,조세인,이철우,정태영,김수흥,김영균 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.5

Platelet-rich plasma is an autologous source of platelet-derived growth factor and transforming growth factor beta that is obtained by sequestering and concentrating platelets by gradient density centrifugation. We have used platelet-rich plasma for bone graft, especially allobone graft, at implant surgery, sinus lift procedure, and cyst enucleation. This article is retrospective study from October 1999 to November 2000. All cases were good healing and clinical success.

급성 심근경색증을 동반한 혈전성 혈소판 감소성 자반증 1예

이태희,김진혁,김완중,박지영,강흥선,조정휘,김권삼,송정상,배종화 대한순환기학회 2003 Korean Circulation Journal Vol.33 No.11

마이크로 센서에의 응용을 위한 SnO₂_(-x)열산화 박막의 특성

김태훈,정완영,홍영호,백종무,최만식,이덕동 경북대학교 센서기술연구소 1994 센서技術學術大會論文集 Vol.5 No.1

SnO_(2-x) thin films were prepated by thermal evaporation of metal tin granules on silicon wafer and were oxidized under various furnace termperature in exposure to O_(2). Mobility, carrier concerntration and resistivity of the SnO_(2-x) thin films were measured by Hall effect measurement and 4-point probe method. The temperature profile in thermal oxidation showed great effects on electric properites and surface structures of the thin films. The thin films were used for the sensing membrane of C_(4)H_(10) microsensor. The contact characteristics of Pt electrode-SnO_(2-x) and sensing properties of the films were investigated.

Boxing 선수들의 체중조절 실태에 관한 조사 연구

김태완,조경욱,이충섭 忠北大學校 平生體育硏究所 1991 平生體育硏究所 論文集 Vol.5 No.-

The investigation of resarch on research on the actual condition about weight control of 137 high school or college boxing players in cheongju, Seoul, Incheon, Suwon, Yongin, and Daegu drew the following conclusions. 1) The methods of weight control were described that reducing weight was 78.15, increasing weight was 13.1% and reducing or increasing weight was 8.8%. The method of imcrease in quantity of exercise was most used as the method of reducing weight, and the methods of bathing and dieting were used a lot. 2) The best condition was in teducing 3-1Kilograms of weight and the players felt the most fatigue in reducing 5-6Kilograms of weight. The foodstuffs which they ate most in teducing weight were vegetable and fruit. Water and soft dinks weten secodly. 3) They described 6-10days before thore t heir games were the most appropriate periods of reducig weight. Many plauers were satified with methods of reducing weight, but 82.5% of them thought reducing weight was icjurious to their tealth. 4) 47.4% of them lost their games because of the failure of reducing weight. All the players described the success of reducing weight affected the victory and thd defeat of their games. Therefore we could understand weight control was another game. 5) 8% of players had experience in taking medicine in reducing weight. 30% of them had experience in reducing more 9Kilograms of weight. "Laxis" was described as the most medicine taken in reducing weight.

웅접흄이 환기기능에 미치는 영향

문태인,구정완,정치경 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.3

In order to study the effects of welding fume on ventilatory function, forced expiratory spirogram was performed on 563 male workers exposed to welding fume in motor vehicles industry and 589 male control workers. Ventilatory indices such as forced vital capacity (FVC), forced expiratory volume in one second (FEV₁), the ratio of one-second forced expiratory volume to forced vital capacity(FEV₁%=FEV₁/FVC) and maximal mid-expiratory flow(MMF) were obtained by analyzing forced expiratory spirogram which were measured by Vitalograph in standing position. The result were as follows: 1. Mean values of FVC, FEV₁, FEV₁%, and MMF expressed as percent of the predicted were significantly lower in exposure group than in control group. 2. No significant differences for mean ventilatory indices were demonstrated between smokers and nonsmokers both in exposure group and control group. However, mean MMF of welders tended to reduce in smokers(77.4%) than in nonsmokers(82.4%). 3. In exposure group mean FEV₁was significantly lower in more than 20 years than those of control group. However, mean MMF, FEV₁% were significantly reduced with increasing exposure duration compared to control group, indicating 90.8% and 62.2% of the predicted in more than 20 years group. 4. In exposure group workers with obstructive ventilatory impairment(7.5%) was markedly higher than that with restrictive ventilatory impairment(2.0%). 5. In exposure group the proportion of workers with low ventilatory indices was the highest in MMF(26.8%), and followed by FEV₁(11.9%), FEV₁(7.6%) and FVC(2.1%) in descending order. Workers with low MMF and FEV₁% were significantly increased with increasing exposure duration compared to control group, indicating 64.3% and 32.1% in above 20 years group. With the above consideration in mind it suggested that the exposure to welding fume be associated with reduced pulmonary function and obstructive ventilatory impairment, increasing exposure duration be associated with reduced pulmonary function, and MMF be the most sensitive index in the evaluation of ventilatory impairments of workers exposed to welding fume.

방광이행상피세포암에서 p53유전자의 변화

김법완,권태균,노기석,정성광,장세국,정운복,김정완 경북대학교 병원 1997 경북대학교병원의학연구소논문집 Vol.1 No.1

The objective of this study was to characterise the pattern of p53 mutations in bladder turmor. In this study, 25 bladder transitional cell carcinomas were analyed by immunohistochemistry (IHC) for p53 nuclear overexpression, and the results were compared with those of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis in exon 5-8 of the p53 gene and DNA sequencing analysis. 15 out of 20 cases (75%) showed p53 nuclear immunoreactivities on IHC. On PCR-SSCP analysis, 10 out of 25 cases(40%) had abnormal shifts on mobility. 62% of the mutations were in exon 8. Direct DNA sequencing analysis were performed in these 10 cases to confirm the presence of mutated p53 genes and to determine the type of mutations. Sixteen point mutations were detected in 10 cases. Tow specimens had double mutations and another two had triple mutations. G:C→A:T transitions were the most frequent patterns (62.5%). One mutation was a premature stop codon and two were silent mutations. There out of 10 had a point mutation at codon 285 (GAG/Glu→AAG/Lys) and two had at codon 280(GAG/Glu→AAG/Lys). One of 16 mutations was transition at hot spot codon 273 with CpG site. These results suggest that altered expressions and point mutations of p53 occured in all grade of bladder cancer, but are more associated with hight grade bladder tumors. To elucidate the carcinogenesis of bladder cancer, further studies should be carried out.

경막외에 침범한 과립구성 육종 1예

최완영,김인순,최일영,정태준,이영열,고영혜,김남규 대한내과학회 1992 대한내과학회지 Vol.42 No.4

본 저자들은 백혈병으로 진단 받지 않은 환자에서 경막외에 침범하여 척수를 누르고 있고 수술후 방사선 치료로 증상이 좋아진 과립구상 육종을 경험하였기에 이에 보고하는 바이다. Granulocytic sarcoma is an uncommon tumor composed of immature cells of the granulocytic series, occuring in patients with AML and blast crisis of CML. It may rarely precede an acute myelogenous leukemia with an uniformly fetal course. We experienced a case of granulocytic sarcoma of extradural space presenting as spinal cord compression in a 31 years old female with no evidence of leukemia on the bone marrow and periphera1 blood. The tumor was located to the T₁-T₃ having narrowing pole after decompression laminectomy, patient received local irradiation to the residual tumor bed, and being followed at the OPD without evidence of leukemia.

하악 무치악 인공치아매식술시 하치조신경 전위술

최의환,하정완,김수관,정태영,김수흥 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.3

Modified surgical technique for transposition of the inferior alveolar nerve followed by immediate placement of endosseous implants in mandibles with moderate to severe atrophy are presented. Five transpositions of the inferior alveolar nerve together with the installation of 10 implants were performed in four patients. The mean postoperative follow-up time was 17 months, with a range of 8 to 20 months. All implants with functioning pontics remained stable, with no mobility or symptoms of pain and infection during the follow-up period. Neurosensory evaluation was performed using the two-point discrimination test. Two patients had objective neurosensory dysfunction at postoperative, but all the nerve function were reported as normal by the patients 4 months postoperatively.