차량 재도색용 청색 페인트의 분석

박성우,손성건,박하선,박성지,정영호,홍성욱,박종신 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

Effect of Non-animal-Derived Nitrogen Sources on the Production of Hyaluronic Acid by Streptococcus sp. KL0188

Lee, Gil-Yong,Ha, Suk-Jin,Jung, Jong-Hyun,Seo, Dong-Ho,Park, Jong-Yul,Kim, Su-Rin,Park, Nam-Woo,Kweon, Dong-Keon,Park, Sang-Hoo,Park, Cheon-Seok The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.3

Hyaluronic acid (HA) is a linear high-molecular-weight polysaccharide with useful biomedical applications. Streptococcus zooepidemicus, a typical HA-producing bacterium, requires an animal-derived nitrogen source such as tryptone, peptone or sheep blood as a nutrient. Sixteen non-animal-derived (NAD) nitrogen sources were tested as a replacement for the expensive animal-derived nitrogen sources, which may have safety issues. Among the sixteen tested NAD nitrogen sources, a yeast-derived nitrogen source (YE 0251) showed the highest HA productivity, which was equivalent to the control HA production medium containing tryptone in a 5-L jar and in 3,000-L industrial fermentations. In the 3,000-L fermentation, YE 0251 increased cell mass (dry cell weight) and HA production by 11% and 8%, respectively, compared with the control HA production medium. The fmal specific volumetric productivity (0.41 g/L h) was improved by about 70% after reducing the fermentation time from 20 h to 12 h, compared to the conventional production medium.

The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection

Chun, Yoon-Keun,Ha, Joo-hun,Hong-Jung-Woo,Oh, Soo-Myung,Kim, Sung-Soo 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yoon-Keun Chun¹,Joohun Ha□Hong-Jung Woo□, Soo Myung Oh□,Sung Soo Kim□ ¹Department of Molecular Biology, College of Medicine,²Department of Surgery, college of Medicine,³Department of Internal Medicine, College of Oriental Medicine,and ⁴East-Weat Medical Reserch Institute,Kyung Hee University, Seoul, Korea. The HBV DNA Amounts in Serum Have No relationship with ALT level and Hetergeneous Population Coexits in Chronic Hepatitis B Virus Infection. Proceedings of International Symposium on East-West Medicine, Seoul. 212-230, 1999. -Hepatitis B is caused by hepadnavirus. Hepatitis B virus replicates through 3.5kb pregenomic RNA intermediate which is regulated by core promoter. Pathogenesis of hepatitis B virus has been bilieved the result of host immune response. But recently many studies have reported that high level of viral replication caused by mutation in core promoter might result in severs hepatitis. But these studies were performed in vitro, not in vivo. So there is yet debate about which factor, viral of host factor, is more important in pathogenesis of hepatitis B virus. So we measured real viral replication level in 204 chronic hepatitis B patients by quantifying HBV DNA from sera by our novel PCR-based more sensitive method, and compared these results with ALT level measured from same sera, which indicates liver cell damage. Surprisingly there are no significant correlation between HBV DNA quantity and ALT level. Then we cloned core promoter region. In SSCP, we found that many viral mutants coexist in one patient. Base on SSCP result, we chose main viral core promoter type in each patients, which is thought to determine overall viral replication level in this patient. Main type of core promoter region of each 41 patients were directly sequenced. And with these we measured promoter activity by luciferase assay system and compared promoter activity with on another. We found tha there were some differences in promoter activity according to core promoter sequences. And we constructed replication-competent viral constructs with core promoter from 41 patients and Transfected these into HepG2 cell and measured HBV DNA by southern blot. There were also differences in HBV DNA quantity according to core promoter sequences. On these all results we investigated correlation between the effect of HBV core promoter on viral replication in vitro and HBN DNA quantity, ALT level from sera of each patients. We found there is no significant correlation among them. As a result, we concluded that in determining severity chronic hepatitis B patients, host factors of each patient is more important rather than replicative activity of virus itself.

Bloodstream Infections and Clinical Significance of Healthcare-associated Bacteremia: A Multicenter Surveillance Study in Korean Hospitals

Son, Jun Seong,Song, Jae-Hoon,Ko, Kwan Soo,Yeom, Joon Sup,Ki, Hyun Kyun,Kim, Shin-Woo,Chang, Hyun-Ha,Ryu, Seong Yeol,Kim, Yeon-Sook,Jung, Sook-In,Shin, Sang Yop,Oh, Hee Bok,Lee, Yeong Seon,Chung, Doo The Korean Academy of Medical Sciences 2010 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.25 No.7

<P>Recent changes in healthcare systems have changed the epidemiologic paradigms in many infectious fields including bloodstream infection (BSI). We compared clinical characteristics of community-acquired (CA), hospital-acquired (HA), and healthcare-associated (HCA) BSI. We performed a prospective nationwide multicenter surveillance study from 9 university hospitals in Korea. Total 1,605 blood isolates were collected from 2006 to 2007, and 1,144 isolates were considered true pathogens. HA-BSI accounted for 48.8%, CA-BSI for 33.2%, and HCA-BSI for 18.0%. HA-BSI and HCA-BSI were more likely to have severe comorbidities. <I>Escherichia coli</I> was the most common isolate in CA-BSI (47.1%) and HCA-BSI (27.2%). In contrast, <I>Staphylococcus aureus</I> (15.2%), coagulase-negative <I>Staphylococcus</I> (15.1%) were the common isolates in HA-BSI. The rate of appropriate empiric antimicrobial therapy was the highest in CA-BSI (89.0%) followed by HCA-BSI (76.4%), and HA-BSI (75.0%). The 30-day mortality rate was the highest in HA-BSI (23.0%) followed by HCA-BSI (18.4%), and CA-BSI (10.2%). High Pitt score and inappropriate empirical antibiotic therapy were the independent risk factors for mortality by multivariate analysis. In conclusion, the present data suggest that clinical features, outcome, and microbiologic features of causative pathogens vary by origin of BSI. Especially, HCA-BSI shows unique clinical characteristics, which should be considered a distinct category for more appropriate antibiotic treatment.</P>

Holographic Interferometry를 이용한 탄성 고무 인상재의 체적변화에 관한 연구

하치양,이성복,우이형 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.1

This study investigated the time-dependent dimensional changes of elastomeric impression materials using holographic interferometry. Six commercial impression materials, Permlastic(polysulfide), Xantopren VI, (condensation silicone), low and medium viscosity of Exafine, Provil (addition silicone), and Impregum( polyether), were selected. Steel plate was used as custom tray, and each impression specimen was 20 mm in width, 15 mm in length and 3 mm in thickness. Each impression material was evaluated at 30 minutes, 1, 2 4, 6, 8 and 12 hours after setting using real-time holography. The results were as follow 1. Xantopren VL and Permlastic showed relatively severe and continuous dimensional changes after setting. Low viscosity of Exafine, Provil, Impregum showed relatively slight dimensional changes with function of time and medium viscosity of Exafine showed almost no dimensional change from 2 hours after setting to 6 hours. 2. On initial dimensional changes within 1 hour, the amount of change in low viscosity of Exafine was the least and Xantopren VL was the largest. 3. On dimensional changes at 4 hours after setting, the amount 'of change in medium viscosity of Exafine was the least and the change of Xantopren VL was the largest. 4. In overall dimensional stability during 12 hours, medium viscosity of Exafine was the most stable and Xantopren VL and Permlastic were least stable.

水稻增收에 關한 硏究

河浩成,金明燦,金正敎,朴愚喆 진주농과대학 1968 진주농과대학 연구논문집 Vol.- No.7

本試驗 結果 米穀 增收의 要件으로 客土와 深耕에 依한 作土層을 增加시킴과 아울러 有機物의 含量을 높이고, 따라서 水?生育에 重要한 要素로 알려진 珪酸質 肥料의 施用 및 不足 ?類를 보충함과 同時에 灌排水 管理 및 耕種關係를 合理的으로 함으로서 現在의 平均 玄米收量 보다 높은 數値의 結果를 얻었다. (1) 客土를 反當 7,500kg 程度 施用함과 有機物 및 ?類 補充으로 莖數, 穗數가 10%, 玄米收量이 12% 增加하였다. (2) 深耕에 依한 作土層의 깊이를 10∼12cm에서 18cm 程度 깊게 함과 同時에 有機物 및 ?類補充으로 客土와 같은 傾向의 成績을 얻었다. (3) 客土와 深耕을 같이 處理한 結果, 客土, 深耕, 單用區에 比하여 莖數, 穗數의 增加는 약간 보았으나 收量面에서는 크게 增加를 보지 못하였다. 이는 當年에 客土, 深耕으로 作土層을 增加시켰다 하드라도, 土壤의 熱度가 잘 이루어 지지 못하였기 때문에 야기된 것으로 본다. (4) 二條竝木區로 坪當 株數를 늘이므로써 收量增加는 認定되지 않았다. 이는 3의 경우와 같다고 하겠으나 他區 역시 坪當 株數가 90株였기 때문으로 본다. To study about an increased yield of rice, this experiment was carried out at five county selected in Kyung Nan province. The essential conditions of increased yield obtained from this experiment were as follows: 1) Supplying with red clay soil to 7,500kg per 10are some of organic matter and mineral nutrition into each field, the stem and ear was increased in number to ten per cent and an unpolished rice to twelve per cent. 2) The coincident results were obtained in deep plowing treatment in order to plow more deep the sub-soil floor of 10∼12㎝ to 18㎝ with supplement of organic matter and mineral nutrition. 3) When the above mentioned two treatments were done together, the stem and ear was moderately increased in number compare with each if the depth of sub-soil was increased according to the supplement of red clay soil and decper plowing, howerer, the soil was not efficiently development during one year for plant to grow well. 4) The two strips planting to increase the number of plant per 0.245 acre was not increased in yield of an unpolished rice. It was seems that the reason also was same as in 3., and that the number of plant was epually ninety in the other all experimental treatment.

상하악 전치부 치열궁 형태에 대한 새로운 접근 : 한국성인 정상교합자 모델에서

하만희,손우성,양훈철 대한치과교정학회 2001 대한치과교정학회지 Vol.31 No.3

상하악 전치부 치열은 치아의 형태 변위, 선천결손 등에 의해 종종 교합관계나 심미성에 문제점을 나타내게 된다. 이러한 문제점을 극복하기 위해 임상의는 전치부 비율을 진단시 이용하게 되나, 치열중 형태, 견치간 폭경(intercanine width), 치열궁 장경(segment depth)과 치열중 둘레(arch perimeter)에 따른 전치부 비율의 변화로 인해 이러한 비율을 전치부 교합관계 예측에 직접 적용하는데는 한계가 있다. 이에 본 연구에서는 한국성인 정상교합자 모델(남자 : 20쌍, 여자 : 20쌍)에서 상하악 전치부 치열궁 형태를 least square method로 조사하였다. 한국인 정상교합자의 상하악 전치부 치열궁 형태는 다항 함수(polynomial function), 베타 함수(beta function), 하이퍼볼릭 코사인 함수(hyperbolic cosine function) 순으로 곡선 접합(curve fitting)하였으며, 이러한 곡선 접합도는 남녀, 상하악에 관계없이 일정하였다. 또한 곡선접합(curve fitting)된 치열궁 형태를 바탕으로 견치간 폭경(intercanine width), 치열궁 장경(segment depth)과 치열궁 둘레(arch perimeter)간의 상관관계를 구하였다. 이러한 상관관계는 견치간 폭경에 따른 치열궁 형태 예측과 보다 정확한 전치부 비율에 대한 정보를 제공할 것이다. (주요 단어 : 전치부 치열궁 형태, 전치부 비율) Maxillary and mandibular anterior dental arches often have the problems of occlusal relation and esthetics by malformations of teeth, congenital missing, et at. Though the clinician usually use the anterior ratio to overcome this problems, he has the limitation of a direct application this ratio to the prediction of anterior occlusal relationship by the change of anterior ratio as dental arch form, intercanine width, segment depth and arch perimeter. So this study examine maxillary and mandibular anterior dental arch forms by least square method using Korean normal occlusion models(man : 20 casts, woman : 20 casts), Maxillary and mandibular anterior dental arches of Korean normal occlusion models are curve fitted to polynomial function, beta function, hyperbolic cosine function in order. And this accuracy of curve fitting is constant regardless of man/woman and maxilla/mandible. The relationships between intercanine width, segment depth, and arch perimeter based on this curve fitted dental arch form are acquired. This relationships will give the prediction of anterior dental arch form and the information of more accurate anterior ratio according to intercanine width. Key words : Anterior dental arch form, Anterior ratio

피로하중에 의한 NITINOL의 기계적 성질의 변화

하국봉,손우성 대한치과교정학회 1993 대한치과교정학회지 Vol.23 No.4

Nitinol wires are now widely used in the orthodontic field because of their unique shape memory effect and superelasticity. But sometimes Nitinol wires are deformed in clinical use. Fatigue load is possible cause of Nitinol deformation. To determine the effect of fatigue load to the mechanical properties of Nitinol, various fatigue cycle(1×10^4, 2×10^4, 3×10^4, 5×10^4, 1×10^5) were applied to 0.017×0.025 inch Nitinol. The results obtained were as follows ; 1. Applied load increased as fatigue cycle increased in three point bending test. 2. Maximum tensile strength and elongation decreased as fatigue cycle increased. 3. In SEM,brittle fracture pattern was increased when fatigue cycle increased.

관광호텔 勞使關係의 安定化 방안에 관한 연구

하종명,이우상 진주여자전문대학 1997 論文集 Vol.20 No.-

The purposes of this study are to find out various significant factors impinging on the stability of labor-relations and their potential effects, and to propose effective approaches for enhancing the stability of labor-relations in tourist hotels. Specifically, the management system of tourist hotels and the empliyees' characteristics were selected as independent variables, and the stability of labor-relations was employed as subordinate variables. For these purposes, hypothesis was established as follows: Relation between independent variables(employees' characteristics) and the subor-dinate variable of the stability of labor-relations(labor harmony) should show significant difference. The samples of this study were 57 hotels in Seoul, Pusan, Taigu, TaiJun, Kyeongju, and Kyeongnam province. Enhancement of employees' Job attitude: the higher efficacy and pride the employees have toward their hotels, the higher degree of committment they may show to their Jobs, thus leading to lower rate of turnover and higher level of Job satisfaction among employees. Optimal labor-harmony may be secured when the employees are willing to sacrifice for the growth of their hotels. As a prerequisite for this harmony, new knowledge, skill, information which are required for employees' job performances must be provided to guarantee efficient communication.

담도에 국한되어 증상을 유발한 석회쓸개즙 2 예

윤성우,이지영,하경호,이수경,한나,배효근,박선자 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Limy bile is a rare condition characterized by excessive precipitation of calcium carbonate in the gallbladder or bile duct. Recently we experienced two cases with obstructive jaundice in which limy bile in the common bile duct but not in the gallbladder for one case, and in the common bile duct and intra hepatic duct but not in the gallbladder for another case. The patients were treated by endoscopic sphincterotomy. Here, we report the cases and review the literature.