Detection of Individuals Restoring Fertility by DNA Fragment Converted into STS (sequence tagged site) in Red pepper

Lee,Jang-Soo,Lee,Keun-Hyang,Kim,Yeo-Song,Kang,Kwon-Kyoo,Nou,Ill-Sup,Hirata,Yutaka 한국자원식물학회 2004 Plant Resources Vol.7 No.2

Co-segregation of male fertility with DNA markers selected by RAPD analysis as being potentially linked to the restorer gene (Rf) for Cytoplasmic male sterility (CMS) was analyzed using segregating F2 population. One RAPD marker directly linked to the Rf locus was identified. Amplification of OPT-02/570 using the STS primers generated a monomorphic band of each fertile plants randomly selected F2 progenies. From these results, this specific marker would be strongly linked to be restoring gene. The use of STS marker is effective in overcoming the reliability of the RAPD phenotype and improving their utility for MAS, co-dominant STS markers are especially very useful.

核然料에 關한 硏究 : α-UO_3의 構造的 特性 Structural Characteristics of α-UO_3

李秀一,曺秉旭,洪光俊 조선대학교 기초과학연구소 1984 自然科學硏究 Vol.7 No.1

α-UO_3 have been prepared from uranyl acetate solution by the thermal decomposition method. The materials structure were examined by surface morpholgy, X-ray diffraction and optical density spectrum. The results showed that α-UO_3 exists two different crystalline forms depending on the temperature of precipitation ; hexagonl α-UO_3 at 350℃ and orthorhombic α-UO_3 at 600℃.

Clozapine 투여로 유발된 무과립구증 1례

이기철,정홍경,이정호,최영민,전성일,최수전 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

저자들은 clozapine 투여 29일이 경과한 시점에서 무과립구증을 보인 20세 여자환자 1례를 치험하였기에 문헌고찰과 함께 보고하였다. 무과립구증은 무균처치와 항생제투여 및 G-CSF와 GM-CSF를 투여하여 무과립구증 상태는 8일만에 회복되었다. Clozapine에 의해 유발된 무과립구증의 원인은 아직까지 확실치 않다. 현재까지 알려진 원인으로는 MHC(major histocompatibility complex region)의 우성인자가 원인이라는 제안과 대사산물이 자유기근으로서 골수세포를 억제한다는 설이 제안되고 있다. 저자들은 clozapine을 투여함에 있어서 환자의 선정, 위험인자들의 고려 및 철저한 CPMS 관리를 제안하고, 일단 무과립구증이 발생하면 즉각적으로 내과적인 도움을 받을 것을 권장한다. We report a 20-year-old female patient who developed clozapine-induced agranulocytosis on the 29th day of clozapine treatment. She recovered from the agranulocytosis on the 8th day of progression after treatment with G-CSF, GM-CSF, antibiotics and associated aseptic procedures. The cause of clozapine-induced agranulocytosis is still un known and it is proposed that the dominant gene of major histocompatibility complex region and the reactive metabolites which suppress the myeloid system may be responsible. We recommend that careful attentions such as the selection of patient, evaluation of the risk factors, and the thorough control of CPMS should be paid. If the agranulocytosis happens, it is helpful to consult to the department of medicine immediately.

忠南地域 屠畜豚의 肺病變으로부터 分離한 Actinobacillus pleuropneumoniae의 生物學的 및 免疫學的 特性

이종훈,장경수,장치훈,김일택,정용성,김귀현,전무형 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

An epidemiologic study on pleuropneumonia in the slaughter pigs(Chonan and Asan area, Chungnam province, Korea) during the period of January 1994 through December 1995 was conducted. Isolation of A. pleuropneumoniae was attempted in 425 pigs with pneumonic lesions. Biochemical properties, antimicrobial susceptibility, serotypes and pathogenicity of A. pleuropneumoniae isolates were investigated. In addition, outer membrane protein(OMP) of the isolates were extracted to determine its properties and immunogenicity in both mice and piglets. Of 3,395 slaughter pigs, pleuropneumonia was observed in 425 pigs(10.6%). A. pleuropneumoniae was isolated from 22 pigs(5.2%) out of 425 pigs with pneumonic lesions. The biochemical properties of all isolates were same as those of reference A. pleuropneumoniae strain. Among 22 isolates, 9, 1 and 12 isolates were serovar 2, 3 and 5, respectively. The results of antimicrobial susceptibility test revealed that the isolates showed high susceptibility to ciprofloxacin and cephalothin, moderate susceptibility to amikacin, gentamicin, kanamycin and streptomycin, and low susceptibility to erythromycin, tylosin and sulfadimethoxin. The isolates were varied in pathogenicity to mice. Median lethal dose of LE9402(serovar 2) and LE9511(serovar 5) were 9.2×10^7CFU and 2.8×10^7CFU, respectively. Specific pneumonic lesions were observed from the infected mice with clinical signs. Bacteria recovery rate was high in the lung, but low in heart blood and tracheas. Serovar 2 was found to be more pathogenic than serovar 5 in guinea pig. Mortality on guinea pigs inoculated with serovar 2(5.4×10^8 - 5.4×10^6CFU) abd serovar 5(2.8×10^8 - 2.8×10^6CFU) was 20-40% and 40-80%, respectively. A severe hemorrhagic lesions and focal pneumonic lesions were observed from dead guinea pigs. Bacteria recovery rate was relatively higher in the lung than that of other organs. In the SDS-PAGE analysis, OMP-enriched fractions of both isolates and reference strains contain common peptide bands equivalent to molecular weight of 17, 27, 42, 52 and 95Kd. In addition to common peptide bands, the bands which are specific to each isolate were also observed. The profiles of Sephadex G25 fractions showed 3 major peaks. The common peptide bands which were observed by SDS-PAGE of the crude OMPs were found i the peaks 1 and 2. The OMPs extracted from serovar 2(LE9402) and serovar 5(LE9511) provided high level of protection in mice(70-80%) and pigs(100%). All animals inoculated with OMPs were seroconverted, showing micro-agglutination titer of 640 to 1280.

Hybrid Breeding of Red Pepper (Capsicum annum L.) Using Cytoplasmic Male Sterile Lines Combined with Phytophythora Resistance in Korea

Lee,Jang-Soo,Kang,Kwon-Kyoo,Hirata,Yutaka,Nou,Ill-Sup,Thanh,Vo-Cong 한국자원식물학회 2004 Plant Resources Vol.7 No.2

We characterized the resistances such as to waterlogging, Phytophythora and viruses in hybrids between Italian and Korean mother lines and screened them for complex resistances and agronomic traits to select elite multi-resistant lines for hybrid breeding. Resistance to waterlogging was selectable due to diversity of the resistance. Phytophythora resistance introduced from Italian lines could also be combined with resistance to other diseases and restoration abilities from cytoplasmic male sterility that has been maintained in Korean varieties.

여우 빙의(憑依) 1례

이병욱,김무진,이수일 大韓神經精神醫學會 1989 신경정신의학 Vol.28 No.5

The authors experienced a 50 year-old female patient who manifested so-called fox possession syndrome of 3 days duration, showing fox-like behavior, idea of being possessed and auditory hallucination. She had a recent episode of transient hysteroid dream state with automatic behavior. She was a highly suggestible shamanistic believer, and recently suffering from emotieonal crisis accompanied by frustration, helplessness, loneliness and hostility. Her symptoms were spontaneously subsided without any specific therapeutic intervention.

섬진강 서시천의 수달서식처 적합성에 관한 어류학적 가치 연구

이승휘,신일수 호남대학교 산업기술연구소 2005 산업기술연구논문집 Vol.12 No.-

용접공들의 면역능에 관한 연구

이수일,조봉수,김영욱,고광욱,조원근,김영실,강수용,황인경,조병만,김돈균 大韓産業醫學會 1997 대한직업환경의학회지 Vol.9 No.1

The authors examined human immunity indicators such as CD4, CD8, Natural Killer(NK) cell, IgG, IgA, and IgM, and heavy metals such as Pb, Cr, Cd to evaluate the health hazard of welders. We selected total 126 male subjects, 60 welders who had more than 5 year working history as an exposure group, and 66 office workers and medical college students as a control group. This immunity evaluation approach is the first newly designed study that has never been tried in past studies on welder's heath evaluation. And the results were as follows. 1. Total lymphocyte count of peripheral blood was significantly higher in welder group, 2,615±650 ea/㎕ compared with 2,368±681 ea/㎕ in control group(p>0.05). 2. The proportion of CD4, CD8, NK cell in welder group was 37.5±9.4%, 24.0±8.8%, and 21.0±9.5%, respectively, and 35.5±6.5%, 25.0±6.9%, and 19.6±8.8% in control group. CD4 and NK cell were slightly higher, and CD8 was slightly lower in welder group. but there was no statistical significance between two groups(p≥0.05). 3. IgG, IgA, and IgM in welder group were 15.9㎎/㎗, 2.7㎎/㎗ and 1.5㎎/㎗, respectively, and 15.6㎎/㎗, 2.9㎎/㎗ and 1.7㎎/㎗ in control group. There was no statistical significance between two groups(p≥0.05). 4. Blood lead, cadmium and chromium concentrations were 4.2±1.8㎍/㎗, 0.4±0.38㎍/㎗, 1.2±0.6㎍/㎗ in welder group and 3.2±1.7㎍/㎗, 0.44±0.29㎍/㎗, 1.1±0.6㎍/㎗ in control group, respectively, and these value were within normal ranges.

AutoCAD 프로그램을 이용한 자동 펌프설계 시스템 개발

김일수,정영재,이창우,박주석 한국공작기계학회 2002 한국생산제조학회지 Vol.11 No.1

Recently, industry has moved towards automated operations with the goal of achieving better product quality, greater productivity and reliability. The pump design is characterized by extensive utilization of the related database which contains performance data. The inputs to the system are through interactive dialogue sessions and the basic input consist of flow rate, head of fluid, efficiency and the customer special requirements. These basic inputs along with the numerous rules in the knowledge bases and the mathematical modelling enable the effective design of the pump industry. This paper represents the development of an automatic pump design system that was composed of a main program, the data input module, the drawing module, the drawing edit module, and was programed y the AutoLISP language under the AutoCAD program. The developed system ultimately generates the design for a pump through the AutoCAD language.

대퇴 골두 골내 결절종 : 증례 보고 A Case Report

임수재,천동일,우승한,임강철,박종석,이병일 대한고관절학회 2004 Hip and Pelvis Vol.16 No.1