Early Phase of UVB - induced GM - CSF Upregulation in Epithelial Cell Line is not Totally Dependent on IL - 1α

Park, Kyoung Chan,Kim, Kyu Han,Ahn, Jong Seong,Chung, Jin Ho,Youn, Jai Il,Whang, Ji Hwan,Youn, Sang Woong,Kim, Young Gull,Koh, Woo Seok,Jung, Hyun Chae 대한피부과학회 1997 Annals of Dermatology Vol.9 No.4

Backgrounds : It was demonstrated that ultraviolet(UV) B light induces the release of IL-la in cultured human epithelial cell line and augmentation of GM-CSF production by UVB is reported to be mediated by IL-1α in the murine keratinocyte cell line Pam 212. Objective : The purpose of this study was to evaluate the effects of UVB on kinetic profile of IL-1 and GM-CSF mRNA expression and to see whether synthesis of GM-CSF by UVB can be completely inhibited by blocking IL-1α mediated pathway. Method : We used a competitive RT-PCR for measuring cytokine gene expression in epithelial cell line after UV radiation. Results : The IL-1α mRNA increased as early as 1h after UV irradiation, and then decreased at 3h after the irradiation. Thereafter, the response of IL-1α mRNA was upregulated with a second peak at 6h after the UV irradiation. However, mRNA for GM-CSF increased at 1h after UV light exposure and anti-IL-1α antibodies could only partially inhibit UV-augmented GMCSF production. Conclusion : UVB induced GM-CSF production seemed to be mainly mediated by UVB induced IL-1α but these results suggest that UVB may also induce GM-CSF production through an IL-1α independent pathway.

The enhanced IL-l8 production by UVB irradiation requires ROI and AP-1 signaling in human keratinocyte cell line (HaCaT)

Cho, Daeho,Kang, Jae Seung,Park, Jong Hoon,Kim, Young-In,Hahm, Eunsil,Lee, Junechul,Yang, Yoolhee,Jeon, Junho,Song, HyunKeun,Park, Hyunjeong,Kim, Taesung,Pang, Saic,Kim, Chul-Woo,Hwang, Young Il,Lee, 전남대학교 약품개발연구소 2002 약품개발연구지 Vol.11 No.-

Based on our recent observation that enhanced IL-18 expression positively correlates with malignant skin tumors, such as SCC and melanoma, we examined the possible role of UVB, known to be associated with skin cancer development, in the enhancement of IL-18 production using primary human epidermal keratinocytes and human cell line HaCaT. After cells were exposed to UVB irradiation in vitro, IL-18 production was examined by Northern blot analysis and ELISA, and it was found that IL-18 production is enhanced by UVB irradiation in a dose- and time-dependent manner. In addition, we confirmed that it is functionally active form of IL-18 using the inhibitor of caspase-1. The effect of UVB irradiation was blocked by antioxidant, N-acetyl-ι-cysteine (NAC), which suggested the involvement of reactive oxygen intermediates (ROI) in the signal transduction of UVB irradiation-enhanced IL-18 synthesis. We also found that UVB irradiation increased AP-1 binding activity by using EMSA with AP-1-specific oligonucleotide. Furthermore, inhibitors of UVB-induced AP-1 activity, such as PD98059, blocked enhanced IL-18 production, indicating that AP-1 activation is required for UVB-induced IL-18 production. Taken together, our results suggest that UVB irradiation-enhanced IL-18 production is selectively mediated through the generation of ROI and the activation of AP-1.

Intracellular IL-4, IL-10, and IFN-gamma levels of leukemic cells and bone marrow T cells in acute leukemia.

Park, Hun Hee,Kim, Myungshin,Lee, Bong-Hee,Lim, Jihyang,Kim, Yonggoo,Lee, Eun Jung,Min, Woo Sung,Kang, Chang Suk,Kim, Won Il,Shim, Sang In,Han, Kyungja Institute for Clinical Science] 2006 Annals of clinical and laboratory science Vol.36 No.1

<P>The quantitative levels of intracellular cytokines IL-4, IL-10, and IFN-gamma (ie, the number of bound PE-conjugated antibody molecules/cell) of leukemic cells and bone marrow T cells (bmT cells) of acute leukemia patients were analyzed by flow cytometry. One hundred, thirty-one (95 AML, 25 ALL, 11 ABL) patients were studied. The leukemic cell IL-4 level was highest in the monocytic AML group (1735 +/- 1056) and lowest in the dysplastic AML group (960 +/- 545). The IFN-gamma level was highest in the acute promyelocytic leukemia (APL) group (495 +/- 159), and lowest in the ALL group (252 +/- 119). The IL-10 level was not significantly different among the diagnosis groups. In bmT cells, the IL-10 level was highest in the dysplastic AML group (972 +/- 1049) and lowest in the APL group (397 +/- 352). The leukemic cell cytokine levels were lowest and bmT cell cytokine levels were highest in the dysplastic AML group. There were no significant correlations of these cytokine levels with 2-yr survival rate, complete remission (CR) rate, or relapse rate. The cytokine levels of bmT cells at the time of CR became normal and were not different among the diagnosis groups. In summary, leukemic cell and bmT cell cytoplasmic expression profiles of IL-4, IL-10, and IFN-gamma are characteristic for each diagnostic group of acute leukemia patients and the profiles of bmT cells are normal at the time of CR.</P>

Regulation of interleukin-11 expression in ovulatory follicles of the rat ovary

Jang, You-Jee,Park, Jae-Il,Jeong, Seong-Eun,Seo, You-Mi,Dam, Phuong T. M.,Seo, Young-Woo,Choi, Bum-Chae,Song, Sang-Jin,Chun, Sang-Young,Cho, Moon-Kyoung Commonwealth Scientific and Industrial Research Or 2017 Reproduction, fertility, and development Vol. No.

<P> The aim of the present study was to examine the regulation of interleukin (IL)-11 expression, as well as the role of IL-11, during ovulation in gonadotropin-primed immature rats. Injection of equine chorionic gonadotropin (eCG), followed by human CG (hCG) to induce superovulation stimulated expression of the Il11 gene in theca cells within 6 h, as revealed by northern blot and in situ hybridisation analyses. Real-time reverse transcription-polymerase chain reaction analysis showed that the IL-11 receptor, α subunit gene was expressed in granulosa and theca cells and that injection of hCG had no effect on its expression. IL-11 protein expression was stimulated in theca cells by hCG. LH-stimulated increases in Il11 mRNA levels in cultured preovulatory follicles were inhibited by protein kinase A and mitogen-activated protein kinase kinase inhibitors. Toll-like receptor (TLR) 2 and TLR4 were detected in preovulatory follicles, and the TLR4 ligand lipopolysaccharide, but not the TLR2 ligand Pam3Cys, increased Il11 mRNA levels in theca cells, but not in granulosa cells. Treatment of preovulatory follicles with IL-11 stimulated progesterone production and steroidogenic acute regulatory protein (Star) gene expression. Together, these results indicate that IL-11 in theca cells is stimulated by mitogen-activated protein kinase signalling and TLR4 activation, and increases progesterone production during ovulation. </P>

푸른길 공원의 대기 환경 특성에 관한 연구

민경우,이경석,박옥현,윤관주,김도술,박세일,정원삼,이대행,조영관,Min, Kyoung-Woo,Lee, Kyoung-Soek,Park, Ok-Hyun,Yoon, Kwan-Ju,Kim, Do-Sool,Park, Se-Il,Jeung, Won-Sam,Lee, Dae-Hang,Cho, Young-Gwan 한국환경보건학회 2015 한국환경보건학회지 Vol.41 No.3

Objectives: This study aimed to survey the characteristics of air quality and meteorological conditions in a greenway park. Methods: We measured meteorological and health related factors, including noise, particulate matter ($PM_{10}$) and selected gaseous air pollutants at three locations in a greenway park and on a general roadside as comparison. The measurements were repeated four times from April to October 2014. Results: The average air temperature in the greenway park was $20.7^{\circ}C$ which was $1-2^{\circ}C$ lower than on the general roadside. The average $PM_{10}$ concentration in the greenway park was $85.0{\mu}g/m^3$, a level 2-3 times lower than that at the roadside. The noise level at the greenway site was 4.4 dB(A)- 23.0 dB(A) lower than at the roadside. The average CO, $CO_2$, $SO_2$ and NOx concentrations in the greenway park were lower than at the roadside. The average phytoncide and anions concentrations in the greenway park were higher than at the roadside. Conclusions: The urban forest of the greenway park may have some impact on air quality and meteorological conditions.

의약품의 효능별 안전성·유효성 평가에 관한 조사연구(Ⅰ) : 항균제 Antiboiotics

박윤주,최기환,김동섭,박인숙,정면우,임화경,오우용,강주희,박찬웅,김주일 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

새로운 물질이 개발되어 신약으로 탄생하기까지 많은 시간과 노력, 예산이 필요하다. 최근, 우리 나라에서도, 신약캐발이 어릴고 힘든 분야이지만 새로운 신약개발을 총해 얻어지는 분가가치가 막대함을 인식, 연구에 박차를 가하 있는 실정이다. 본 연구는, 의약품 개발 선진국인 미국 FDA에서 발행하는 참고자료 중 항균제 임상편가에 대한 가이드라인 및 주요 임상적응증별 항균제 기발(지역사획회득성 폐렴, 원내감염성 계렴, 급성기관지염의 2차세균감염, 만성기지염의 급성세균성 악화, 연쇄구균에 의한 인두영 및 편도염 , 단순성 요로감염증, 복합성 요로감염증 및 신우신영, 급성 세균성 부비동옆, 항 바이러스제 개발시 전임상단계에서 고려사항, 항 바이러스제 허가와 된련 잉상적 고려사항, 카레타 괸련 혈류감염)에 있어서의 파이드라인 등의 자료를 소개함으로져, 향후 제약사의 항균제 신약개발 및 허가 신청된 의악품의 전임상차료 및 잉상시험자료의 검토 평가업무에 참고자료로 활용될 수 있을 것이다. Changing or unclear interpretations of clinical trial data needed to demonstrate the effectiveness and safety of antimicrobial drug products have a times led to confusion and frustration on the part of both applicants and division of these drug products reviewers. The FDA published guidance on desing clinical protocols, implementing, and analyzing data from clinical trials for antimicrobial drug products has been presented and additional companion guidances introduced specific issues to individual infections. This document provides applicants and reviewers with minimal information appropriate for the clinical development of routine antimicrobial drug products and identifies issues common to many antimicrobial drug applications. The agency can use the kind of information to determine whether the antimicrobial drug product under study is safe and effective in the treatment of the specific infection studied.

Amelioration of inflammatory responses by <i>Socheongryong-Tang</i> , a traditional herbal medicine, in RAW 264.7 cells and rats

Park, Sang Mi,Lee, Tae Hoon,Zhao, Rongjie,Kim, Youn Sook,Jung, Ji Yun,Park, Chung A.,Jegal, Kyung Hwan,Ku, Sae Kwang,Kim, Jae Kwang,Lee, Chul Won,Kim, Young Woo,Cho, Il Je,An, Won G.,Kim, Sang Chan D.A. Spandidos 2018 International journal of molecular medicine Vol.41 No.5

<P>Socheongryong-Tang (SCRT) is a natural medicine prescription that has been mainly used in East Asia for the treatment of inflammatory disorders, including asthma and allergic rhinitis. The present study evaluated the anti-inflammatory effects of SCRT on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and in a rat model of carrageenan (CA)-induced paw edema. Levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) in the culture supernatant were quantified and nitric oxide (NO) production was monitored. In addition, the effect of SCRT on the protein expression of nuclear factor-κB (NF-κB), mitogen-activated protein kinases (MAPKs), inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) was assessed by western blot analysis. Furthermore, the effects of SCRT on acute inflammation <I>in vivo</I> and changes in the histomorphometry and histopathology of paw skin were observed using CA-treated rats. SCRT (1 mg/ml) inhibited the LPS-induced changes in the protein expression of NF-κB, JNK, ERK1/2, iNOS and COX-2, as well as the production of NO, PGE<SUB>2</SUB> and cytokines. In the rat paw edema assay, administration of 1 g/kg of lyophilized powder obtained from the aqueous extracts of SCRT for 3 consecutive days inhibited the CA-induced increases in skin thickness, mast cell degranulation, and infiltration of inflammatory cells in the ventral and dorsal pedis skin within 4 h. These results demonstrated that SCRT exerts its anti-inflammatory activities in LPS-stimulated RAW 264.7 cells through decreasing the production of inflammatory mediators, including PGE<SUB>2</SUB>, NO and cytokines, via suppression of the NF-κB and JNK and ERK1/2 signaling pathways. In addition, the data of the CA-induced paw edema indicated an anti-edema effect of SCRT. SCRT (1 g/kg) reduced acute edematous inflammation through inhibition of mast cell degranulation and infiltration of inflammatory cells. Therefore, the present study provided scientific evidence for the anti-inflammatory activities of SCRT as well as the underlying mechanisms.</P>

Anti-inflammatory Activity of an Ethanol Extract of Caesalpinia sappan L. in LPS-induced RAW 264.7 Cells

Il Yun Jeong,Chang Hyun Jin,Yong Dae Park,Hyo Jung Lee,Dae Seong Choi,Myung Woo Byun,Yeung Ji Kim 한국식품영양과학회 2008 Preventive Nutrition and Food Science Vol.13 No.4

The anti-inflammatory activities of an ethanol extract of Caesalpinia sappan L. (CS) were investigated in LPS-induced RAW 264.7 cells. Result indicated that CS inhibited the LPS-induced NO production in a dose-dependent manner with an IC?? of 10.9 ㎍/mL. In addition, CS attenuated the iNOS mRNA and protein expression by inhibiting NF-κB activation. CS also suppressed the productions of IL-6 and MCP-1 in a dose-dependent manner, with IC?? values of 15.9 ㎍/mL and 5.47 ㎍/mL, respectively. In addition to the anti-inflammatory activities, CS decreased intracellular ROS formation in the same cells. In conclusion, CS inhibited the production of NO, IL-6 and MCP-1 via a suppression of the NF-κB activation and intracellular ROS generation.

월아산 근린공원의 식생구조와 동태

노일,조현남,박우진,문현식 경상대학교 농과대학 부속연습림 2001 演習林硏究報告 Vol.- No.11

The objectives of this study was to investigate the vegetation structure and dynamics in the urban neighborhood park of Mt. Weola. The results obtained were as follows; 1. The woody plants of 18 species are distributed in upper story. By importance value, the upper story was dominated in the order of Pinus densiflora, Qoercus variabilis, Robinia pseudoacacia, Platycarya strobilacea, Alnus firma, Quercus aliena, Carpinus laxiflora. 2. In middle story is formed by 23 species with dominated in the order of Rhus trichocarpa, Prunus sargentii, Quercus variabilis, Platycarya strobilacea, Carpinus laxiflora, Quercus serrata by importance value. 3. 68 species is forming the upper story. Importance values of dominated species was in the order of Rhus trichocarpa, Indigofera kirilowii, Lesdedeza bicolor, Quercus variabilis, Smilax china, Lindera glauca. 4. 78 species is distributed in herbaceous story. And, importance value was high in the order of Oplismenus undulatifolius, Carex siderosticta, Disporum smilacinum, Phryma leptostachya var. asiatica, Paederia scandens, Carex humilis, Spodiopogon sibiricus. 5. The species diversity and evenness in each story were 0.751 and 0.598 for upper story, 0.473 and 0.347 for middle story, 1.429 and 0.794 for lower story and 1.125 and 0.594 for herbaceous story, respectively.

16주 저항성 트레이닝이 엘리트 역도선수의 피로물질과 근 손상, 산화적 손상, myokine에 미치는 영향

Cheol-Woo Kim(김철우),Gun-Do Kim(김군도),Sung-Hwun Kang(강성훈),Chan-Hoo Park(박찬호),Kwi-Baek Kim(김귀백),Young-Il Kim(김영일) 한국생명과학회 2012 생명과학회지 Vol.22 No.2

본 연구의 목적은 16주 근력 트레이닝을 동반한 TRX, 코어 훈련이 엘리트 역도 선수의 피로물질과 근 손상, 산화적 손상, myokine에 미치는 영향을 규명하기 위함이다. 본 연구 대상자들은 총 10명(남 6명, 여 4명)으로 구성되어 있으며 16주 근력 운동과 더불어 코어 및 TRX 트레이닝을 실시하여 다음과 같은 결과를 얻었다. Ammonia와 Pi는 트레이닝 전에 비해 점차적으로 증가함을 나타냈으나 통계적으로 유의한 차이는 나타나지 않았다. 근 손상 물질인 CK는 트레이닝 전에 비해 8주에 유의한(p<0.05) 증가를 나타냈으며, LDH는 트레이닝 전에 비해 8주에 유의한(p<0.05) 증가를 나타냈지만 16주에는 감소하는 경향을 나타냈다. 산화적 손상 지표인 MDA는 트레이닝전과 16주에 비해 트레이닝 8주에서 유의한(p<0.05) 증가를 나타냈으며, 항산화 지표인 TAS 또한 트레이닝 전에 비해 트레이닝 8주에서 통계적으로 유의한(p<0.05) 증가를 나타냈다. Myokine인 IL-15는 트레이닝 전에 비하여 트레이닝 8주와 16주에 통계적으로 유의한(p<0.05) 증가를 나타냈다. 결과적으로 16주 고강도 근력 트레이닝은 엘리트 중학교 역도선수의 피로물질과 근 손상, 산화적 손상, myokine에는 긍정적이 영향을 미친 것으로 나타났다. The purpose of this study was to examine the effect of 16 weeks of resistance training on the fatigue factor, muscle soreness, oxidative stress, and myokine in elite weightlifters. A total of 10 subjects (six male, four female) participated in this study. The results were compared according to baseline, 8 weeks, and 16 weeks. Ammonia and Pi were increased through 16 weeks of resistance training, but this result was not significant. CK was significantly (p<0.05) increased at 8 weeks and 16 weeks compared to baseline, while LDH was significantly (p<0.05) increased at 8 weeks compared to baseline. The MDA of the oxidative stress factor was significantly (p<0.05) increased at 8 weeks compared to baseline and 16 weeks, and TAS of the antioxidant factor was significantly (p<0.05) increased at 8 weeks compared to baseline. The IL-15 of the myokine was significantly (p<0.05) increased at baseline compared to 8 weeks and 16 weeks. In conclusion, 16 weeks of high-intensity resistance training may have a positive effect on peripheral fatigue factors, muscle soreness, oxidative stress, and myokine in elite weightlifters.