인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

Curcumin protects retinal pigment epithelial cells against oxidative stress via induction of heme oxygenase-1 expression and reduction of reactive oxygen

Woo, Je Moon,Shin, Da-Yong,Lee, Sung Ju,Joe, Yeonsoo,Zheng, Min,Yim, Jin Ho,Callaway, Zak,Chung, Hun Taeg Molecular Vision 2012 Molecular vision Vol.18 No.-

<P><B>Purpose</B></P><P>To determine whether curcumin induces expression of the defensive enzyme heme oxygenase-1 (HO-1) and protects cells against oxidative stress in cultured human retinal pigment epithelial cells.</P><P><B>Methods</B></P><P>Effective concentrations and toxicities of curcumin were determined after 3 h of curcumin treatment with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Confluent human retinal pigment epithelium cell lines (ARPE-19) were preincubated with curcumin and oxidatively challenged with H<SUB>2</SUB>O<SUB>2</SUB>. HO-1 expression was determined with western blot analysis. To confirm the protective role of HO-1 in oxidative stress, small interfering RNA (siRNA) against HO-1 or inhibitor of HO-1 was treated with curcumin in retinal pigment epithelium cells. Intracellular levels of reactive oxygen species (ROS) were measured with flow cytometry and confocal microscopy. Apoptosis was evaluated with Annexin V-fluoroscein isothiocyanate staining.</P><P><B>Results</B></P><P>Curcumin had little cytotoxicity at concentrations less than 30 μM, and HO-1 expression was the highest at the 15 μM concentration. At this concentration, curcumin also increased the cytoprotective effect against the oxidative stress of H<SUB>2</SUB>O<SUB>2</SUB> through the reduction of ROS levels in human retinal pigment epithelial cells. Curcumin’s effect on the reduction of ROS was mediated by the increase in HO-1 expression.</P><P><B>Conclusions</B></P><P>Curcumin upregulated the oxidative stress defense enzyme HO-1 and may protect human retinal pigment epithelial cells against oxidative stress by reducing ROS levels.</P>

Effects of hydrogen plasma treatment on SnO2:F substrates for amorphous Si thin film solar cells

Min-Seung Choi,Young-Ju Lee,Jung-Dae Kwon,Yongsoo Jeong,Ju-Yun Park,강용철,Pung Keun Song,Dong-Ho Kim 한국물리학회 2013 Current Applied Physics Vol.13 No.8

We investigated the effects of hydrogen plasma treatment on the physical and electrical properties of fluorine-doped tin oxide (FTO) films used for amorphous silicon (a-Si) thin film solar cells. A slight increase in carrier concentration by the hydrogen doping effect was observed for the FTO film exposed to the hydrogen plasma for 5 min. For further exposure to the plasma, the chemical reduction became prominent and resulted in deterioration of the electrical and optical properties of the film. XPS analysis revealed that the chemical reduction of SnO2 to Sn metallic state occurs on the surface region. It was found that the defects formed by hydrogen plasma act as recombination centers at the interface between FTO electrode and p-layer of a-Si solar cells. This phenomenon resulted in the deterioration of the cell performance. The averaged conversion efficiency (6.82%) of the cells on pristine FTO hydrogen substrate was decreased to 5.81% for the cells on FTO treated for 5 min, which is mainly attributed to the decrease in short-circuit current density.

저농도 이산화탄소 포집용 흡착제 개발 및 최적조건에 관한 연구

이주열 ( Ju Yeol Lee ),박덕신 ( Duck Shin Park ),조영민 ( Young Min Cho ),권순박 ( Soon Park Kwon ),황윤호 ( Yoon Ho Hwang ),송형진 ( Hyung Jin Song ),이상봉 ( Sang Bong Lee ) 한국유화학회 2012 한국응용과학기술학회지 Vol.29 No.1

본 논문은 다양한 시설내에 적은 농도의 CO2 제거를 위한 선택적 CO2 흡수능력을 향상시킨 흡착제의 효율평가에 관한 것이다. 직경 4mm의 구형 흡착제는 시판용 제올라이트에 첨가제, 물, 바인더, LiOH를 섞어 제조하였다. 칼럼테스트에서 400분 이내에 90% 이상의 CO2흡착효율을 나타내었고, 흡착필터모듈 흡착능력을 평가하기 위해 회분식과 연속식타입의 챔버테스트가 시행되었다. 회분식테스트에서 30분 이내에 약 92%의 CO2가 제거되는 것을 확인하였다. 연속식테스트에서 30분 이내 70%의 CO2가 제거효율을 보였으며, 2,500ppm 이상의 CO2가 제거되는 것을 확인하였다. 재현성테스트를 수차례 수행한 결과 15일동안 1,000ppm 이상의 CO2가 연속적으로 제거됨을 보였다. TGA 분석법을 이용한 흡착량 분석에서 흡착제 g당 5.0mmol의 CO2를 흡착하는 것으로 나타났다. 본 연구에서 개발된 흡착제는 상온에서 저농도 CO2 실내환경에 적용가능한 것으로 판단된다. In this study, CO2 adsorbent was developed for removing low concentration of CO2 in multiple-use facilities. The efficiency of the adsorbent which was improved selective CO2 adsorption capabilities was evaluated. The pellet type adsorbent was modified from a commercial zeolite with mixing LiOH, binder, additives, and H2O. Column tests showed over 90 % of CO2 was adsorbed within 400min. Chamber tests including batch and continuous types were performed for evaluating the adsorbent module. By batch tests, it was evaluated that about 92 % of CO2 was removed within 30 min. By continuous tests, 70% of CO2 was removed within 30 min. It was analyzed that over 2,500 ppm of CO2 was continuously removed as shown chamber tests. The reproducibility tests repeatedly performed for 15 days shows that over 1,000 ppm of CO2 was continuously removed. Adsorption capacity of the developed adsorbent was 5.0mmol CO2/g adsorbent which was analyzed by TGA. It was estimated that the modified adsorbent was applicable to low CO2 concentration and low temperature of indoor environment.

학교구강보건실 운영 평가에 관한 연구

민희홍,신승철,서현석,김은주,김호선 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.2

School dental clinic programs is the most active and effective programs to prevent the oral disease for school-children and it is one of the recommended programs by the advanced and welfare countries. In Korea, 15 primary schools have been established the school dental clinics in 1999 as one of the public oral health programs supported by the Ministry of Health and Welfares, and expanded more every year. 11 school dental clinics among 15 were participated for 2-years evaluation project. Such items as the structure, the process, the supply of dental services, the promotion of the health level and the community supports were evaluated through the standardization by governmental suggestions. Average score for 2-years evaluation was estimated as 81.35% as considerably good remark. In detail, score 4.74 for the structure, 22.10 for the process, 19.77 for supply of dental services, 26.22 for promotion of the oral health level and 8.53 for the community supports were calculated. In conclusion, school dental clinic programs in Korea were evaluated considerably good and they should be extended to all primary schools as possible.

중증외상환자에서 동반된 척추 및 척수손상에 대한 임상적 분석

정주호,김대용,박창민 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Background: The traumatic spinal injury is an extremely serious condition that often results in death or disabilities. We investigated epidemiological data on spinal injuries specifically in severe trauma patient admitted to a preliminarily proposed Major Trauma Distinctive Care Center. Materials and Methods: We reviewed the medical records of 28 severe trauma patients (Injury Severity Score >15) with traumatic spinal (vertebral column or spinal cord) injuries admitted to the emergency medical center in the Kosin University Gospel Hospital (KUGH) from January to October 2009, retrospectively. Results: The ratio of male to female was 3 to 1. The mean age was 46.5 years. The leading causes of traumatic spinal injury are falls (39.4%). The most common level of injury was cervical (35%) and lumbar (35%) spine. Spinal fracture accounts for 47.8% of all traumatic spinal injury, spinal cord injury 37%, dislocation 8.7% and HNP (herniated nucleus pulposus) 6.5%. The most common type of spinal body fracture was compressive type (58.8%). In spinal cord injury, incomplete injury (64.7%) was more common than complete injury (35.3%) and quadriplegia accounts for 47.1% and paraplegia 52.9%. The most common associated injury was rib fracture & pneumohemothorax (31.2%). 5 patients underwent surgery earlier than 24 hours. The average Injury Severity Score (ISS) was 24.2 and the average Abbreviated Injury Scale Score (AISS) was 3.5. Conclusion: This clinical analysis is the first basic study for understanding the patterns of the spinal injuries in the severe trauma patients admitted to KUGH emergency medical center which was preliminarily proposed as Major Trauma Distinctive Care Center by Ministry for Health, Welfare and Family Affairs in November 2008. Although this study has only small number of cases during relatively short term period, it will be touchstone of succeeding analysis of spinal injuries in the severe trauma patients. Therefore continuous additional data collections and more precise clinical investigations are suggested

IPv6 인터넷 보호 프로토콜에서의 예상 평문 공격에 대한 분석 및 대응책

박현민,소주호 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

Probable Plaintext Attack uses some probable plaintexts to find a secret key using the relationship between a pair of plaintext and ciphertext. IP header have some probable fields which can be used in a probable plaintext attack. Because of address size, Plaintext of IPv6 have more than plaintext of IPv4. In order to reduce the size of a probable plaintext in IPv6, it is required to exclude the address field from the probable plaintext attack. An encryption technique using random numbers is proposed. Before IPSEC mechanism is applied to a packet, the address field is exclusive-ored with a random number, which ensures that the address field is concealed from a probable plaintext attack. This reduces the large amount of a probable plaintext size in IPv6.

토지이용패턴에 따른 하천수질과 식생분포

오영주,강병화,김병우,김성필,한민수,김진호,나영은 한국환경농학회 2006 한국환경농학회지 Vol.25 No.1

운동신경로의 신경학적 고찰

최준호,송준민,김진상 大邱大學校 再活科學硏究所 1994 再活科學硏究 Vol.12 No.1

This study was carried out to investigate the pyramidal and extrapyramidal tracts which were composed of motor tract with respect of neuroanatomy and neurophysiology, for the purpose of increasing the physiotherapical approach on degeneration of motor system, hemiplegia and paraplegia. The results were as follows: 1.The motor tract was composed of pyradidal tract and extrapyramidal tract. 2.The pyramidal tract was composed of corticospinal tract and corticobulbar tract. 3.The extrapyramidal tract was largely composed of vestibulospinal tract, tectospinal tract, reticulospinal tract and rubrospinal tract. 4.The corticospinal tract served predominantly fine and precise movement. 5.The extrapyramidal tract was involved in the coordination of posture and locomotion. 6.The basal ganglia helped determine the direction, force and speed of movement. 7.The cerebellum coordinated posture and movement.

단클론성 IgG 침착을 동반한 증식성 사구체 신염 1예

차정민,정덕은,송주흥,안선호 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

Proliferative glomerulonephritis with monoclonal IgG deposits is a novel phenotype of glomerular injury with the diagnostic criteria of (1) the presence of glomerular monoclonal IgG deposits restricted to a single IgG subclass and a single light chain isotype, associated with endocapillary proliferative, membranoproliferative, or membranous features; (2) the presence of granular("immune complex type") deposits by electron microscopy; and (3) the absence of clinical and laboratory evidence of cryoglobulinemia. We report a case of 65-year-old man with generalized edema who was diagnosed as proliferative glomerulonephritis with monoclonal IgG deposits by renal biopsy.