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사료원료 공급량 및 스크류 회전속도를 달리하여 제조한 배합사료가 넙치(Paralichthys olivaceus)의 성장 및 체조성에 미치는 영향
김경덕 ( Kyoung Duck Kim ),김강웅 ( Kang Woong Kim ),이봉주 ( Bong Joo Lee ),배기민 ( Ki Min Bae ),안철민 ( Cheul Min An ),한현섭 ( Hyun Sob Han ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.5
The aim of this study was to investigate the effect of diet extruder conditions, such as feed-loading rate and screw speed, on growth performance and biochemical responses in olive flounder Paralichthys olivaceus. Over 8 wks, we used four identical diets (triplicated per treatment) with differing ratios of feed-loading rate (kg/h):screw speed (rpm/min) in a laboratory-scaled twin-screw extruder of 50:640, 80:640, 120:640, and 80:400, designated as EP1, EP2, EP3, and EP4, respectively. Screw speed impacted the buoyancy of experimental diets. Diets produced at a screw speed of 640 rpm/min floated for > 24 hrs, whereas those produced at a speed of 400 rpm/min sank between 10 s and 5 min. Fish that were fed EP1 and EP4 diets grew significantly faster than those fed EP2 and EP3 diets. Fish fed EP1 diets ate and gained weight most efficiently among treatments, a result that is likely to be related to feed-loading rate, i.e., ingredients extruded at a low feed-loading rate may have more time to cook in the pre-conditioner of the extruder. A cooked diet may be easier to digest in fish. Fish fed EP4 diets also showed significant weight gain, as compared to those fed EP2 and EP3 diets. However, we found no differences among treatments in proximate compositions of dorsal muscle, liver, and viscera of fish. Our results suggest that extruder conditions, may influence feed quality, impacting feed efficiency and growth of fish.
Kim, Young-Ok,Park, In-Suk,Kim, Dae-Jung,Nam, Bo-Hye,Kim, Dong-Gyun,Jee, Young-Ju,An, Cheul-Min The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5
The selected isolate, Bacillus sp. SW1-1 showed antibacterial activity against both Gram-positive and Gram-negative bacteria involved in fish diseases, including Edwardsiella tarda, Streptococcus iniae, S. parauberis, Vibrio anguillarum, and V. harveyi. The Maximum bacteriocin production was observed at $30^{\circ}C$ after 24 h with brain heart infusion medium (pH 7.0). The bacteriocin SW1-1 was purified by 50% ammonium sulfate precipitation, followed by HiPrep diethylaminoethyl 16/10 FF and Sephacryl S-100 High resolution column chromatography. The substance was characterized as a bacteriocin-like inhibitory substance with a molecular mass of 38 kDa. Bacteriocin SW1-1 was sensitive to the proteolytic action of pepsin, trypsin, chymotrypsin, and protease types I and XIV, and relatively heat labile, despite the fact that bacteriocin activity was still detected after heating at $100^{\circ}C$ for 30 min. The activity of bacteriocin SW1-1 was stable in the pH range of 2.0-11.0, and relatively unaffected by organic chemicals. The bacteriocin SW1-1 had a bacteriolytic mechanism, resulting in cell wall degradation of E. tarda. These characteristics indicate that this bacteriocin may be a potential candidate for alternative agent to control important pathogens of fish diseases in aquaculture.
( Kyung Min Park ),( Ying Li ),( Bora Kim ),( Haiyan Zhang ),( Kyong Hwangbo ),( Dong Gen Piao ),( Mei Juan Chi ),( Mi Hee Woo ),( Jae Sue Choi ),( Je Hyun Lee ),( Dong Cheul Moon ),( Hyeun Wook Chang 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0
Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among `Injinho` and `Myeon-injin` and `Haninjin`--A. capillaris collected in autumn, A. capillaris collected in spring and A. iwayomogi, which can be misused as `Injinho` in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40°C. Calibration andquantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognitionanalysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.
Interleukin-5 저해 활성 chalcone 화합물의 유효 입체 구조
이기철,김민석,P. Thanigaimalai,Vinay K. Sharma,박경래,김영수,정상헌 충남대학교 약학대학 의약품개발연구소 2010 藥學論文集 Vol.25 No.-
Interleukin(IL)-5 appears to be one of the main proinflammatory mediators among the growing number of cytokines and chemokines that induce eosinophilic inflammation. Previously, our group synthesized a number of chalcone derivatives to determine their inhibitory activity against IL-5. However, the structure-activity relationship (SAR) of these chalcones was somewhat unclear, robably due to presence of three free rotatable bonds. To further, explore the SAR of these chalcones, we synthesized eight 2-(benzylidene)-2,3-dihydroinden-1-ones and 2- benzylidene)-3,4-dihydronaphthalen-1(2H)-ones 6 and evaluated for their inhibitory activity against IL-5. These rigidified chalcones 6 exhibited very weak inhibitory activity against IL-5 compared to chalcones 2. The structures of 6 closely resemble to the stretched conformations of chalcone 2, not effective conformation for the inhibition on IL-5 function.
閔潤植,金奇哲 충북대학교 농업과학기술연구소 1992 農業科學硏究 Vol.10 No.1
Prpionibacterium sp. and Lactobacilus sp. were cultured in single and mixed under the condition of S. G. Y. medium with skim milk added with glucose and yeast extract or skim milk whey, and compared conditions of fermentation and acid fermentation by interaction. Propionibacterium 3 strains and Lactobacilus 6 strains, the best strains in growth or organic acid production were P. freudenrecichii KFCC 31227 and L. acidophilus KFCC 32825 which have shown typical symbiosis and commensalism tendency. P. freudenreichii KFCC 31227 and L. acidophilus KFCC 32825 showed better growth in mixed cultuure than in single culture. Production of lactic acid was more in single culture and the production of propionic acid was increased in mixed culture. The optimum condition of acid production was approximate pH 7, temp. 35℃, and 72 culture hr. The heating condition of medium was 100℃, 30 min. The optimum ratio of two kinds of strain starter was 1 : 1.
마르코프 reward 모델을 이용한 통신망의 성능 및 신뢰도 분석 기법
노철우,김경민 신라대학교 자연과학연구소 1998 自然科學論文集 Vol.5 No.-
This paper describes techniques to analyze network reliability and performance using Markov Reward Models(MRM). MRM is obtained by associating reward rates with Markov chain's states. This process facilitates combined evaluation of performance and reliability. Stochastic Reward Net(SRN) is a formalism for a high level specification of MRM. Modeling techniques to construct SRN network reliability models with comparing GSPN models are given. It is shown that reward-based models could lead to more concise model specification and solution of a variety of new measures. With respect to the solution of performance and reliability models, we propose two practical approaches: hierarchichal and phase type expansion modeling and show how reliability/availability measures can be obtained via performance analysis using special reward rates assignment. Two software packages, SPNP and SHARPE, that allow the specification and solution of MRMs are used.
고강도 콘크리트 디스크를 이용한 혼합모드 파괴에 관한 연구
진치섭,김희성,박현재,김민철 부산대학교 생산기술연구소 2000 生産技術硏究所論文集 Vol.59 No.-
RILEM 위원회가 제시한 3점 휨 시험은 하중-변위곡선 형상이 불규칙하고 안정된 균열 발생 후 최종균열이 발생하기 때문에 정확한 파괴인성을 구하는 것은 어렵다. 그러나, 디스크 시험은 균열 개시 하중만 알면 쉽게 파괴인성을 구할 수 있다. 중앙에 노치가 있는 고강도 콘크리트 디스크를 실험하여 실험결과와 유한요소에 의한 결과를 비교하였다. 또한, 실험에 의한 파괴 포락선과 이론에 의한 파괴 포락선도 비교하였다. It is difficult to obtain accurate fracture toughness values by RILEM committees proposed three point bend test(TPB) because the shape of load-deflection curve is irregular and final crack propagation of occurs after some slow stable cracking. However, for disk test, fracture toughness is easily obtained from crack initiation load. We examined the center notched high strength concrete disk and the experimental results were compared with the results by finite element analysis(FEA). Also, we compared experimental
탁한호,이병로,김병철,전중창,황석순,정민우 진주산업대학교 산업과학기술연구소 2003 산업과학기술연구소보 Vol.- No.10
In this paper, applications of adaptive fuzzy controller to position control of flexible beam are considered. When a flexible beam is rotated by a motor through the fixed end, transverse vibration may occur. The architecture and learning underlying adaptive networks based fuzzy inference system (ANFIS) is presented, which is a fuzzy inference system implemented in the framework of adaptive networks. By using a hybrid learning procedure, the proposed ANFIS can construct an input-output mapping based on both human knowledge and stipulated input-output data pairs. Therefor, a dynamic model for a flexible beam is derived, and then a comparative analysis was made with before learning and after learning adaptive fuzzy controller through an simulation. The results are presented to illustrate the advantages and improved performance of the proposed position control cver the after learning adaptive fuzzy controller.