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HOCl Oxidation-modified CT26 Cell Vaccine Inhibits Colon Tumor Growth in a Mouse Model
Zhou, Rui,Huang, Wen-Jun,Ma, Cong,Zhou, Yan,Yao, Yu-Qin,Wang, Yu-Xi,Gou, Lan-Tu,Yi, Chen,Yang, Jin-Liang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8
Despite progress in elucidating mechanisms associated with colorectal cancer and improvement of treatment methods, it remains a frequent cause of death worldwide. New and more effective therapies are therefore urgently needed. Recent studies have shown that immunogenicity of whole ovarian tumor cells and subsequent T cell response were potentiated by oxidation modification with hypochlorous acid (HOCl) in vitro and ex vivo. These results prompted us to investigate the protective antitumor response with an HOCl treated CT26 colorectal cancer cell vaccine in an in vivo mouse model. Administration of HOCl modified vaccine triggered robust antitumor immunity to autologous tumor cells in mice and prolonged survival period significantly. In addition, increased necrosis and apoptosis were found in tumor tissue from the oxidation group. Interestingly, ELISPOT assays showed that specific T cell responses were not elicited in response to the immunizing cellular antigen, in contrast to raising sera antibody titer and antibody binding activity shown by ELISA assay and flow cytometry. Further evaluation of the mechanisms underlying HOCl modified vaccine mediated humoral immunity highlighted the role of antibody-dependent cell-mediated cytotoxicity. These results combined with previous studies suggest that HOCl oxidation modified whole cell vaccine has wide applicability as a cancer vaccine because it can target both T cell- and B cell-specific responses. It may thus represent a promising approach for the immunotherapy of colorectal cancer.
Expression and Characterization of RNA-dependent RNA Polymerase of Dendrolimus punctatus Tetravirus
Zhou, Liang,Zhang, Jiamin,Wang, Xiaochun,Jiang, Hong,Yi, Fuming,Hu, Yuanyang 생화학분자생물학회 2006 Journal of biochemistry and molecular biology Vol.39 No.5
Dendrolimus punctatus tetravirus (DpTV) has been identified as a new member of the genus Omegatetravirus of the family Tetraviridae that may be related serologically to Nudaurelia capensis virus ($N{\omega}V$). To establish the function of DpTV RNA genome and to better understand the mechanism of viral replication, the putative RNA-dependent RNA polymerase (RdRp) domain has been cloned and expressed in Escherichia coli. The recombinant protein was purified on a Ni-chelating HisTrap affinity column and demonstrated to initiate viral RNA synthesis in a primer-independent manner but not by terminal nucleotidyle transferase activity in the presence of $Mg^{2+}$ and RNA template. Mutation of the GDD to GAA interferes with the residues at the polymerase active site and metal ions, and thus renders the polymerase inactive.
Liang Bai,Huilin Wei,Bin Wang,Fangfang Liao,Tianhua Zhou,Xing-Wen Liang 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.47 No.1
This paper presented an investigation into steel tubes encased high-strength concrete (STHC) composite walls, wherein steel tubes were embedded at the boundary elements of high-strength concrete walls. A series of cyclic loading tests was conducted to evaluate the failure pattern, hysteresis characteristics, load-bearing capacity, deformability, and strain distribution of STHC composite walls. The test results demonstrated that the bearing capacity and ductility of the STHC composite walls improved with the embedding of steel tubes at the boundary elements. An analytical method was then established to predict the flexural bearing capacity of the STHC composite walls, and the calculated results agreed well with the experimental values, with errors of less than 10%. Finally, a finite element modeling (FEM) was developed via the OpenSees program to analyze the mechanical performance of the STHC composite wall. The FEM was validated through test results; additionally, the influences of the axial load ratio, steel tube strength, and shear-span ratio on the mechanical properties of STHC composite walls were comprehensively investigated.
Liang Li,Zhang Fengmei,Feng Naibo,Kuang Biao,Fan Mengtian,Chen Cheng,Pan Yiming,Zhou Pengfei,Geng Nana,Li Xingyue,Xian Menglin,Deng Lin,Li Xiaoli,Kuang Liang,Luo Fengtao,Tan Qiaoyan,Xie Yangli,Guo Fen 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Osteoarthritis (OA) is a full-joint, multifactorial, degenerative and inflammatory disease that seriously affects the quality of life of patients due to its disabling and pain-causing properties. ER stress has been reported to be closely related to the progression of OA. The inositol-requiring enzyme 1α/X-box-binding protein-1 spliced (IRE1α/XBP1s) pathway, which is highly expressed in the chondrocytes of OA patients, promotes the degradation and refolding of abnormal proteins during ER stress and maintains the stability of the ER environment of chondrocytes, but its function and the underlying mechanisms of how it contributes to the progression of OA remain unclear. This study investigates the role of IRE1α/ERN1 in OA. Specific deficiency of ERN1 in chondrocytes spontaneously resulted in OA-like cartilage destruction and accelerated OA progression in a surgically induced arthritis model. Local delivery of AdERN1 relieved degradation of the cartilage matrix and prevented OA development in an ACLT-mediated model. Mechanistically, progranulin (PGRN), an intracellular chaperone, binds to IRE1α, promoting its phosphorylation and splicing of XBP1u to generate XBP1s. XBP1s protects articular cartilage through TNF-α/ERK1/2 signaling and further maintains collagen homeostasis by regulating type II collagen expression. The chondroprotective effect of IRE1α/ERN1 is dependent on PGRN and XBP1s splicing. ERN1 deficiency accelerated cartilage degeneration in OA by reducing PGRN expression and XBP1s splicing, subsequently decreasing collagen II expression and triggering collagen structural abnormalities and an imbalance in collagen homeostasis. This study provides new insights into OA pathogenesis and the UPR and suggests that IRE1α/ERN1 may serve as a potential target for the treatment of joint degenerative diseases, including OA.
( Xiaofeng Zhou ),( Jianghui Li ),( Weilong Wang ),( Fan Yang ),( Bingqian Fan ),( Chenlu Zhang ),( Xiaojun Ren ),( Feng Liang ),( Rong Cheng ),( Fengying Jiang ),( Huaibin Zhou ),( Juanjuan Yang ),( 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.7
Various genetically engineered microorganisms have been developed for the removal of heavy metal contaminants. Metal biosorption by whole-cell biosorbents can be enhanced by overproduction of metal-binding proteins/peptides in the cytoplasm or on the cell surface. However, few studies have compared the biosorption capacity of whole cells expressing intracellular or surface-displayed metal-adsorbing proteins. In this study, several constructs were prepared for expressing intracellular and surface-displayed Ochrobactrum tritici 5bvl1 ChrB in Escherichia coli BL21(DE3) cells. E. coli cells expressing surface-displayed ChrB removed more Cr(VI) from aqueous solutions than cells with cytoplasmic ChrB under the same conditions. However, intracellular ChrB was less susceptible to variation in extracellular conditions (pH and ionic strength), and more effectively removed Cr(VI) from industrial wastewater than the surface-displayed ChrB at low pH (<3). An adsorptiondesorption experiment demonstrated that compared with intracellular accumulation, cell-surface adsorption is reversible, which allows easy desorption of the adsorbed metal ions and regeneration of the bioadsorbent. In addition, an intrinsic ChrB protein fluorescence assay suggested that pH and salinity may influence the Cr(VI) adsorption capacity of ChrB-expressing E. coli cells by modulating the ChrB protein conformation. Although the characteristics of ChrB may not be universal for all metal-binding proteins, our study provides new insights into different engineering strategies for whole-cell biosorbents for removing heavy metals from industrial effluents.
李洲良(Zhou-Liang Li) 중국어문논역학회 2014 中國語文論譯叢刊 Vol.0 No.35
Whether the documents of our times or the literature unearthed, we have not found out the record feature of sublime words with deep meaning in Chuen-Qiou in accordance with any material in the pre-Qin period by far. In spite of the following objective conditions such as the troublesome words and strenuous writing instruments are trapped, carapare-bone and copper ware scripts have the style of contracted writings, which has to be simple in order to meet the need of abridged words. The author of Chuen-Qiou has omitted the origin, course and many details in accidents, cutdown the dialogues, speech of characters and interior presentation so as to the abundant vivid historical narrative is evacuated and drained for the descendants to recover. Although the record feature of sublime words with deep meaning has the root with the simple style of carapare-bone and copper ware scripts, which are on purpose in need of profound truth contained in subtle words. The writing of simple diction and appraised meaning formed by record feature in Chuen-Qiou has a direct effect on narrative in tso chuan and Mandarin, as turns in to the basic style of Chinese anciant narrative, that is, Chuen-Qiou writing style.
Temperature Field Reconstruction of Concrete Dams based on Distributed Optical Fiber Monitoring Data
Huawei Zhou,Zhiguo Pan,Zhipeng Liang,Chunju Zhao,Yihong Zhou,Fang Wang 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.5
Mastering the real temperature distribution of the concrete dam is the basis for solving the problem of temperature control and crack prevention. In this paper, Distributed Temperature Sensing (DTS) technology was applied to temperature monitoring of a higharch dam under construction in southwest China. In order to obtain a more comprehensive temperature distribution of the dam, optical fiber layout principles for arch dams were studied, and horizontal and vertical optical fiber layout schemes were first proposed according to these principles. The real temperature variation processes of the dam were obtained in real time with a line temperature measurement pattern instead of a point temperature measurement pattern. Additionally, a framework of distributed optical fiber data acquisition and remote transmission was proposed. Interconnection of multiple DTS hosts and remote transmission of temperature data were realized. Then, two-dimensional temperature fields of a typical dam block in different ages and longitudinal profiles of dam blocks with embedded fibers were reconstructed based on large amounts of temperature monitoring data and the Kriging difference algorithm. Temperature field reconstruction results showed that the temperature distribution law of the concrete arch dam was in accordance with the actual situation.
Zhipeng Liang,Chunju Zhao,Huawei Zhou,Yihong Zhou 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.1
To solve the problems of poor positioning accuracy and non-automatic processing seen in the machining of long ladder shaft gears, a fixture structure scheme with a new type of double collect chuck (DCC) is proposed. The design rationality, clamping stability, and repeat clamping positioning accuracy of the proposed DCC were analyzed and investigated based on theory calculation, simulation analysis, and engineering application tests. First, the clamping performance of DCC was investigated through theory calculation and simulation analysis. The test results showed that the structure of DCC maintained effective clamping and the repeat clamping positioning accuracy was steadily controlled within 0.008 mm. Meanwhile, efficiently automated machining of long ladder shaft gear was realized based on extended design and engineering application of automatic up and down mechanism when the structure of DCC was applied. Furthermore, the machining accuracy of the gear was steadily controlled within DIN8 (DIN5480-2 standard). Additionally, based on extended design and engineering tests, the automatic up and down machining improves production efficiency and machining accuracy. Thus, the novel fixture structure scheme provides a new approach for automatic up and down machining of long ladder shaft gear.