The expression patterns of RANKL and OPG in murine tooth eruption

Hwang, Kyung-Mun,Kim, Eun-Jung,Kim, Hyun-Jung,,Kim, Young-Jin,Nam, Soon-Hyeun 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

치아의 맹출은 치아기 (dental organ)와 치조골의 세포와 연관된 매우 복잡한 과정이다. 우선 치아 맹출이 일어나기 전에 파골세포가 치낭으로 집결하게 된다. 이러한 치낭의 역할은 파골세포와 조골세포의 상호작용으로 이루어 지는 골개조와 밀접한 관련이 있는데, 이는 치아 맹출과 연관된 많은 유전자들이 치낭에서 발현되기 때문이다. RANKL는 TNF ligand family로써 조골세포에 존재하며 파골세포의 형성 및 전구세포로 부터의 활성화를 유도한다. 이러한 RANKL은 OPG에 의해 그 작용이 억제되며 RANKL와 OPG의 상대적인 비율이 파골세포의 형성에 영향을 미친다. 또한 Runx2유전자의 변이는 조골세포의 분화와 활성에 차질을 가져오고 결국 RANKL/OPG pathway를 통해 파골세포 형성에 영향을 줄 수 있다. 치아의 발육 및 맹출에 미치는 RANKL 및 OPG의 영향을 알아보고 Runx2와의 연관성을 알아보기 위해 in situ hybridization 방법으로 태생 1, 3, 5, 7, 9, 11일된 쥐의 하악 및 제1대구치를 사용하여 실험을 실시한 결과 RANKL, OPG, Runx2의 mRNA가 태생 1일부터 11일까지 치낭 및 치아주위조직에 특성있게 나타났다. 이중 태생 5일에서 9일 사이에 RANKL 및 Runx2는 치아의 교합면측과 하방 치조골 부위의 발현이 강하게 나타난 반면 OPG는 약한 발현을 보였다. 이는 또한 파골세포의 활성부위를 알아보기 위해 TRAP염색을 실시하여 태생 5일에서 9일 사이에 최대의 활성화를 나타낸 결과와 연광성 있게 나타났다. RANKL, OPG, Runx2의 특성있는 발현양상들을 종합해 볼 때, 치아 맹출은 치낭, 치아기, 치조골 사이의 상호 작용을 통해 이루어 지며, 이는 치낭이 치아 맹출에 있어서 매우 중요하다는 것을 의미한다. 또한, 이러한 유전자들 (RANKL, OPG, Runx2) 이 치아의 맹출에 중요한 역할을 하는 것으로 사료된다. Tooth eruption is a complex and tightly regulated process that involves cells of the tooth organ and the surrounding alveolus. Osteoclast precursors must be recruited into the dental follicle prior to the onset of eruption. This function of dental follicle may be regarded as the ability of bone remodeling characterized by the interaction of osteoclasts and osteoblasts. This is because tooth eruption is a localized event in which many of the genes required for eruption are expressed in the dental follicle. RANKL is a membrane-bound protein that is a member of the TNF ligand family, which is present on bone marrow stromal cells and osteoblasts, and induces osteoclast formation and activation from precursor cell. The biologic effect of RANKL is inhibited by OPG and, in bone, the relative ratio of RANKL and OPG modulates osteoclastogenesis. To evaluate the roles of RANKL and OPG in tooth eruption and the relations with the expression pattern of Runx2, in situ hybridization was performed with mandibles of mice at postnatal stage 1, 3, 5, 7, 9 and 11. mRNA of RANKL, OPG, and Runx2 are expressed in dental follicle and surrounding tissue from P1 to 11. To determine the sites of osteoclastic activity duing tooth eruption, mandibles were dissected. Peak osteoclastic activity in alveolar bone along the occlusal and basal regions was observed from P5 to 9, with osteoclasts in these regions being large and strongly TRAP-positive. The specific spatic-temporal expression patterns of RANKL, OPG and Runx2 in our study suggest that tooth eruption could be progressed through the interactions of molecular signaling among dental follicle, dental organ and alveolar bone, furthermore it means that dental follicle is quite important in tooth eruption. In addition, it indicates that these genes (RANKL, OPG, and Runx2) play critical roles in tooth eruption.

대구 유니버시아드대회 자원봉사활동에 대한 만족도 연구

황성동,성희자,고강호,전보경,권현수 慶北大學校 社會科學大學 2003 社會科學 Vol.15 No.-

골프 우드 드라이버 스윙 운동 시 에너지 소비량과 운동 강도

황경식,황지현,이사겸 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.2

The purpose of this study is to provide basic data for exercise prescription by investigating for exercise intensity and energy expenditure while swinging golf fairway drive wood club. The subjects of this study are 14 male and female college students. Body composition, VO₂max. HRmax by testing graded exercise test and energy expenditure while 10 minutes golf swing using automatic gas analyzer Vmax 229(sensormedics; U.S.A) and heart rate monitored system Polar system(U.S.A) are investigated. SPSS(Version 11.0) was using as statistical method. Independent sample t-test. For all tests, alpha was set at .05 initially in significance. The results were as follows. Fist, Average heart rate was lower in male as 111.9±7.1beats·min^(-1) then in female as 115.4± 14.8beats·min^(-1). Average percent HRmax was lower in male as 56.1±2.8% then in female as 60.2±8.2. However. there was no significant difference between two groups. Second. Average RPE was lower in mele as 11.7±0.5 than in female as 12.0±0.6. However, there was no significant difference between two groups. Third, Average VO₂ was higher in male as 0.83±0.04ℓ·min^(-1) than in female 0.50±0.111ℓ·min^(-1), Average VO²per kg was higher in male 10.9 7±0.99㎖-kg^(-1)-min^(-1) than in female as 8.74±1.22㎖-kg^(-1)-min^(-1). There were significant differences between two groups. Forth. Average %VO₂max was higher in male as 28.1±3.9% than in female as 26.1±5.6. However, there was no significant difference between two groups. Fifth, Energy expenditure per minute was lower in male as 1.83±0.03kcal-min^(-1). than in female 1.81±0.08kcal-min^(-1). However, there was no significant difference between two groups. Energy expenditure per kg was lower in male as 0.063±0.005kal·kg^(-1)·min^(-1) than in female as 0.086±0.0111kal·kg^(-1)·min^(-1). There was significant difference between two groups. In conclusion, no significant differences were found in exercise intensity of golf swing for male and female subjects. Energy expenditure of female subjects were shown higher than male subjects. Energy expenditure must compared by relative divided by weight(kg) value.

리본 형태의 근관에서 열연화주입법의 근관벽에 대한 적합도에 관한 연구 : ADAPTATION TO CANAL WALLS

황현숙,조경모,김진우 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.4

The aim of this study is to compare the adaptability of thermoplasticized injectable gutta-percha technique to the canal walls in ribbon-shaped canals. Thirty resin models simulated ribbon-shape canals were instrumented to #40 using. 06 taper Profile systems. Three groups of each 10 resin models were obturated by the lateral condensation technique(LC) and the two thermoplasticized injectable gutta-percha technique; Ultrafil Endoset+Obtura Ⅱ(EO) and Ultrafil Firmset(UF), respectively. After resin model were kept at room temperature for 4 days, they were resected horizontally with microtome at 1, 2, 3, 4 and 5mm levels from apex. At each levels, image of resected surface were taken using CCD camera under a stereomicroscope at ×40 magnification and stored. Ratio of the area of gutta-percha was obtained by calculating area of gutta-percha cone to the total area of canal using digitized image-analyzing program. The data were collected then analyzed statistically using One-way ANOVA. The results were as follows. 1. At 1mm levels, there was no statistically significant difference in the mean ratio gutta-percha among the groups. 2. At 2mm level, EO showed the highest mean ratio of gutta-percha (p<0.05) and there was no significant difference between LC and UF. 3. At 3, 4, 5mm levels, EO and UF and had significantly greater mean ratio of gutta-percha than LC(p<0.05) and there was no significant difference between EO and UF. In conclusion, the thermoplasticized injectable gutta-percha techniques demonstrated relatively favorable adaptability to canal walls than lateral condensation technique in ribbon-shaped canals except for 1mm level.

이중혈류유발 심폐소생술이 심정지를 유발한 개의 단기 생존율에 미치는 영향

황성오,조준휘,강구현,김성환,문중범,이강현,이승환,윤정한,최경훈,홍은석 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

Background and Objectives: We previously reported that, compared with standard cardiopulmonary resuscitation(S-CPR), better hemodynamic effects could be achieved by simultaneous sterno-thoracic cardiopulmonary resuscitation(SST-CPR) in which we compressed the sternum and constricted the thorax circumferentially during the systolic period by using a device. This study was designed to assess whether SST-CPR, compared with S-CPR, improve the survival rate of dogs with cardiac arrest. Subjects and methods: Twenty-five mongrel dogs(19∼31㎏) were enrolled in this study. After four minutes of ventricular fibrillation induced by an AC current, animals were randomized to resuscitate with either S-CPR(n=13) or SST-CPR(n=12). Epinephrine(1 ㎎) was injected into the right atrium every three minutes after the beginning of CPR. Defibrillation was attempted after 6 minutes of CPR. Standard advanced cardiac life support was started if defibrillation was not successful. Results: SST-CPR resulted in significantly(p<0.001) higher systolic arterial pressure(91±47 vs 47±24 ㎜ Hg), diastolic pressure(43±24 vs 17±10 ㎜ Hg), coronary perfusion pressure(35±25 vs 13±9㎜ Hg), and end tidal CO2 tension(9±4 vs 3±2 ㎜ Hg). Two of 13 animals(15 %) resuscitated with S-CPR and six of 12 animals(50%) resuscitated with SST-CPR survived until 12 hours after cardiac arrest(p<0.05). Donclusion: SST-CPR, compared with S-CPR, improves the short-term survival rate in canine cardiac arrest.

두가지 혈류 유발방법에 의한 새로운 심폐소생술(이중 혈류 유발 심폐소생술)장치의 개발

황성오,김현,조준휘,오범진,임종천,최경훈,윤정한,이승환,김영식,이강현,이윤선 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: There have been many efforts to augment blood flow during cardiopulmonary resuscitation. These efforts have focused on maximizing the effect of cardiac pump or thoracic pump alone. However, considering that the heart is the biggest blood reservoir and increase of intrathoracic pressure can generate blood flow, simultaneous exploitation of both mechanisms may have synergistic effect. We hypothesized that simultaneous chest constriction in addition to sternal compression by standard CPR may have additive hemodynamic effects by preventing deformation of the chest and increase of intrathoracic pressure. Methods and results: we built a new mechanical device to perform compression and thoracic constriction simultaneously. The device consists of two main elements. Piston in the center is to depress the sternum. Strap is to constrict the thorax circumferentially. Strap is attached to both sides of the piston. When the piston is pushed down, it depresses the sternum and pulls on the thoracic strap. To determine strap width to produce optimal hemodynamic effect, we measured hemodynamic parameters with variable widths of strap in two dogs after induction of ventricular fibrillation. Result of the experiment showed that 10cm wide strap was determined to be most effective. We also determined optimal depth of compression to produce maximal hemodynamic effect with animal experiments using two dogs. Animal experiments showed that the highest aortic pressure could be generated when the stemum was depressed to 5 cm. Cardiopulmonary resusciation using a new device could generate higher systolic aortic pressure, coronary perfusion pressure and end-tidal carbon dioxide tension in comparison with standard cardiopulmonary resuscitation in a pilot animal study using two dogs. Conclusion: New cardiopulmonary resuscitation method using a mechanical device designed by us could perform sternal compression and simultaneous thoracic constriction, and generate better hemodynamic effects than standard cardiopulmonary resuscitation in pilot animal experiments.

영아보육교사의 전문성 인식과 역할수행에 관한 연구

황경애,김현주 진주산업대학교 2005 論文集 Vol.44 No.-

The purpose of this study is to find out the degree of the Educare Center Teachers' recognition of their role as professionals and to what degree this recognition influences their actual role performance. The subjects of this study were 126 infant nursing teachers located in Jinju. The data was analyzed by t-test and ANOVA in SPSS program. The results are as follows : 1) First, professional recognition degree of infant nursing teachers appeared to be over average in overall. The result from examining professional recognition in terms of circumstance factors of the infant nursing teachers indicated significant differences depending on experiences, education in overall, where as indicated no difference depending on age, major, facility type, working hours and payment. 2) Second, In the realm of role performance, age and experience were a signigicant factor. 3) Third, as a rule, it was shown that there was intimate correlation between the recognition and the performance.

가사노동의 사회화에 관한 이론적 고찰

황경애,안현숙 진주산업대학교 1993 論文集 Vol.32 No.-

The purpose of this study is to review the preceding research related to housework socialization. Following items are reviewed: 1) the concept of housework socialization 2) the pattern of housework socialization 3) relations to demographic variables 4) relations to psycho-social variables 5) domains of housework socialization

생활양식과 소비자역할 인식 및 기능과의 관계에 대한 연구

황경애,안현숙 진주산업대학교 1993 論文集 Vol.32 No.-

The purpose of this study is to investigate lifestyle pattern and to analyze the influence of demographic and psycho-social valuables on consumer role concept and ability. For the purpose of this study, questionnaires were administrated to 319 housewives in Chinju. The data were analyzed by factor analysis, One-Way ANOVA, Pearson's correlation, Multiple Regression Analysis. The major results of this study were summarized as follows: 1) By utilizying factor analysis the life style patterns of housewives were classified 8 types. 2) The variables which were influenced directly on consumer role concept ; rational activity type, sound and thrifty type, income. (These variables explain consumer role concept 18%) 3) The variables which were influenced on consumer role concept ; rational activity type, social activity type, modernistic self type, number of child, occupation of husband. (These variables explain 37.2%) 4) Relations between consumer role concept and consumer role ability are positive correlation. (r=.24, P<.001)

장기투석을 받은 만성 신부전 환자에서 발생한 종양에 관한 고찰

황정화,이혜경,홍현숙,박재성,김대호,권귀향,최득린,황승덕,이희발 순천향의학연구소 1996 Journal of Soonchunhyang Medical Science Vol.2 No.2

The authors tried to evaluate tumor occurrence in long-term dialysis patients with chronic renal failure. Among 359 patients, 20 patients (about 5.6%) were diagnosed with malignancy during long-term dialysis from the period of 1983 to 1995 at our nephrology department. The ultrasonographic and computed tomographic findings including the clinical features of 20 patients that were retrospectively reviewed. The mean age of the patients was 53 (37-75)years old and the ratio of male to female was 9:1. Among the 20 cases, 7 cases of hepatoma (35%) were developed. Among them, urinary tract tumors such as renal and bladder cancer were developed in 4 (20%) and 2 (10%) of the cases. Other malignant tumors were lymphoma, stomach cancer, uterine cervical cancer, cholangiocarcinoma, lung cancer, meningioma, and acoustic neuroma one case of each (each of 5.3%). The most common tumor in patients with chronic renal failure, who were receiving long-term dialysis, was hepatoma and the second most common tumor was cancer of the urinary tract such as kidney and bladder.