맥문동 물 추출물의 식이가 Streptozotocin으로 유도한 당뇨 흰쥐의 혈당과 혈청 콜레스테롤 함량에 미치는 영향

임정교,강명수,박인경,김순동 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.1

The dietary effect of water extracts of Liriopis tuber(WELT) in the diabetic SD-rats on the level of blood sugar and serum cholesterol was investigated. The experimental plots divided into normal group(N), diabetic control(DC), 5% WELT-group(WELT-Ⅰ) and 10% WELT-group (WELT-Ⅱ). Each group was fed for 6 weeks, then continuously fed for 1 more week after streptozotocin injection. The loss of the body weight fed for one week after induction of the diabetes was 2.2~6.3% in the WELT-Ⅰ and -Ⅱ groups, but it was 18.6% in the DC-group. There was no significant difference in the feed intakes after diabetes induction between N-group and WELT-group, while it was significantly increased in DC-group. The feed efficiency ratios before diabetes induction were 1.70 in WELT-Ⅰgroup, 1.53~1.59 in the N, DC and WELT-Ⅱ group, while the ratios after diabetes induction were 0.92 in DC-group, 1.51~1.83 in the N, WELT-Ⅰ and -Ⅱ group. While the amounts of water intakes for one week after diabetes induction was 625.4 mL in the DC-group, and it were 364.3~371.1 mL in the WELT-groups showing no significant difference with N-group. The excretion amounts of urine were 431.96 mL in DC-group for one week after diabetes induction, and it was 182~192.84 mL in WELT-groups. The ratios of liver weight against body weight were 2.74% in N-group, 2.93~2.96% in WELT-groups, but it was 4.01% in DC-group. The level of blood glucose in WELT-groups fed for one week after diabetes induction were 136.8~138.6 ㎎/dL showing no significant difference with N-group, but it was 357.8 ㎎/dL in DC-group. The level of serum triacylglycerol and serum total cholesterol were 93.8 and 68.7 ㎎/dL in N-group, 120.1 and 101.6 ㎎/dL in DC-group, 97.4~100.6 and 60.8~67.7 ㎎/dL in WELT-groups, respectively, showing no significant difference between N-group and WELT-groups. HDL-cholesterolltotal cholesterol ratio were 0.63 in N-group, 0.57~0.67 in WELT-groups, which was significantly higher than that of DC-group(p<0.05). Atherogenic index were 0.58 in N-group, 0.49~0.74 in WELT-groups, but it was 1.32 in DC-group. The above results suggest that the WELT diets may have both preventive and curing effects against the diabetes.

단백질-다당질 복합겔의 용해도 및 전기영동 특성에 미치는 몇가지 첨가제의 영향

정경임,이동선,하정옥 경남대학교 신소재연구소 1996 論文集 Vol.6 No.-

본 연구에서는 닭고기에서 조제한 근원섬유 단백질(MP)과 MP와 유청 단백질 분리물(WPI)의 혼합물, MP와 대두 단백질 분리물(SPI)의 혼합물 등의 3가지 종류의 단백질과 다당질로서 methyl cellulose, sodium alginate, xanthan gum을 택하여 단백질-다당질의 복합겔을 만들고 NaCl, NaSCN, propylene glycol, 2-mercaptoethanol들의 처리에 의해 단백질-다당질간의 상호작용에 기인하는 겔의 용해도 및 전기영동에 의한 겔 용해에 따른 상징액의 변화 등을 조사함으로써 단백질 식품의 기능적 특성에 미치는 영향을 고찰해 보았다. 겔 용해도는 대체로 모든 다당질에 대해 낮은 용해도를 나타냈으나 세 가지 단백질 시료에 대해 2-mercaptoethanol 처리시 가장 많은 단백질이 용해되어 나온 것으로 나타났다. 2-mercaptoethanol은 단백질간 상호작용, 즉 겔 망상구조를 이루는 sdisulfide 결합을 크게 방해함으로써 가장 높은 용해도를 나타낸 것으로 생각된다. 모든 시료에 대해 NaCl과 NaSCN 용액에 대해서는 아주 낮은 용해도를 보였는데, 이것은 NaCl과 NaSCN은 저 농도일 경우, 단백질 구조를 안정화함으로써 단백질간의 상호작용이 크게 증가되어 용해되는 단백질의 양이 극히 낮아진 것으로 보인다. Xanthan gum은 가장 겔 용해도가 낮았고, 특히 MP와 xanthan gum으로 이루어진 겔에서는 아주 극소량의 단백질이 용해되었다. Methyl cellulose 겔의 경우, 모든 용액에서 단백질이 거의 용해되어 나오지 않았다. 그러나, 1% WPI를 혼합한 경우 역시 겔 용해도가 낮았으며 여전히 MP의 단백질 성분은 용해되어 나오지 않았고 WPI의 주요성분인 α-lactalbumin과 β-latcoblobulin이 많이 용해되어 나왔다. 1% SPI을 혼합한 겔은 셀 용해도도 여전히 낮았으며 전기영동 결과에서도 SPI 성분의 단백질 band가 아주 미약하게 나타났다. 이것으로 보아 WPI는 다소 불안정한 겔을 형성하여 용해도를 증가 시키고 반대로 SPI는 겔을 안정화하여 단백질 용해도를 감소시켰으리라 짐작된다. Sodium alginate 겔의 경우, 단백질 용해도는 아주 낮았으나 3 가지 단백질 시료 모두 용해되어 나왔다. 이것으로 sodium alginate는 단백질과 다소 불안정한 겔을 형성한다는 것을 알 수 있다. X무소무 gum 겔에서도 역시 MP와 MP+SPI 혼합겔은 아주 낮은 겔 용해도를 뒷받침하듯 단백질 band를 거의 확인할 수 없었으나 WPI를 혼합한 겔만이 WPI의 주요성분인 α-lactalbumin과 β-latcoblobulin 성분이 용해되어 나왔다. 즉, methyl cellulose와 xsanthan gum 겔의 경우, 근원섬유 단백질은 거의 용해되어 나오지 않았고 유청 단백질 분리물의 성분이 많이 용해되어 나왔으며 대두 단백질 분리물은 단백질과의 결합력이 커 용해된 단백질 성분이 아주 미약했다. Sodium alginate 겔의 경우 모든 단백질 성분이 용해되어 나온 것으로 보아 sodium alginate는 단백질과 결합하여 불안정한 겔을 형성하는 것으로 나타났다. Interactions between polysaccharides and proteins occur widely in biological system. Their interaction in food system often plays a role in determining the functional properties of these systems. The effects of various reagents such as NaCl. NaSCN. propylene glycol and 2-mercaptoethanol on the gel solubility of heat-induced gels of protein and polysaccharide were studied. Protein samples were myofibrillar proteins(MP) extracted from chicken breast muscle and the combinations of MP plus whey protein isolate(WP1) and MP plus soybean protein isolate(SP1). As polysaccharides in preparation of gels, methyl cellulose, sodium alginate and xanthan gum were used. Protein- polysaccharide composite gels were formed by heating at 80 C for 30 min. In the effects of reagents on gel solubility, 2-mercaptoethanol had higher solubility than other reagents. indicating that 2-mercaptoethanol cleaved disulfide bonds in the protein-polysaccharide composite gels. In order to indicate the composition of protein solubilized from gels by the treatment of various reagents such as 0.1 M NaC1. 0.1 M NaSCN. 20% propylene glycol, 0.04 M 2-mercaptoethanol, the solubilized proteins were analyzed by SDS-PAGE. In methyl cellulose and xanthan gum gels. less protein was solubilized by reagents, but MP gels with WPI was solubilized more abundantly than SPI.

가정관리연구에서의 체계론적 접근과 인간생태학적 접근에 대한 검토

임정빈,임혜경 漢陽大學校 韓國生活科學硏究所 1991 韓國 生活 科學 硏究 Vol.- No.1

The purpose of this paper is to suggest the usefulness of systematic / ecological approach as means of facilitationg the development of home management research. Home management not only reflect the dynamic changes within a household but also the less obvious interchanges between the household and the larger environment. The total managerial effect is greater than the sum of the individual components. So, the study of home management should reflect this wholeness, or totality. According these ideas, firstly the basic concepts of general system theory and human ecology are explained, and discussed the appropriateness of two approaches in home management study. As a result, systematic and ecological approach in home management research are both necessany. Systematic approach is more useful in analysis of stuctural and systematic problem to manage home efficiently. Ecological approach is more available to study dynamic and multidimensional family living.

바질 물추출물을 첨가한 두부의 품질특성

임정교,박인경,김순동 한국조리과학회 2004 한국식품조리과학회지 Vol.20 No.2

This study was conducted to investigate the quality characteristics and shelf-life of tofu added with basil water extract(BWE). BWE was selected from 11 kinds of herbs based on estimations of antimicrobial activity, taste and flavor. The amount of waster was 7 times treater than that of raw soybean, BWE concentration against soybean juice was 20㎎% and GDL concentration was 0.3% as the optimal preparation conditions of tofu added with BWE(BWE-tofu). Color L*, a* and b* values of BWE-tofu were 78.11, 0.78 and 19.10, respectively, indicating that light green color was lower in the L* value, and higher in the a* and b* values compared to control products. Savory taste and overall acceptability of the tofu were higher than those of control products evaluated by sensory test. Shelf-life of the tofu at 10℃ was 8 days, which was prolonged by 4 days compared to control products evaluated by pH, titratable acidity and total microbes.

상아질 결합제의 L929 세포 독성에 관한 연구

임미경,지정호,김상섭 大韓齒科保存學會 1997 Restorative Dentistry & Endodontics Vol.22 No.1

세균에 노출된 말초혈액 단핵구에서 Interleukin-8과 Monocyte Chemoattractant Protein-1의 형성에 관한 연구

임미경,이정민 大韓齒科保存學會 2000 Restorative Dentistry & Endodontics Vol.25 No.2

남자 한복바지구성의 상징성 연구 : 圖式을 중심으로

정옥임,김경희 服飾文化學會 2003 服飾文化硏究 Vol.11 No.3

When analyzing fors in the human body and clothes, we conceptualize each part into a cone, sphere, and cylinder, and the esthetic characteristics of our fashion culture and esthetic fashion characteristics of Koreans are composed by expressing with universal images such as circle, square, and triangle when forms are expressed in a plane. To determine how the structural principle of shapes that are the shapes of all things and basic to geometry, i.e., circle, square, and triangle, applies to clothes that cloth our body called the small cosmos, we discussed the symbolic meaning hidden inside the structure of Korean clothes based on the basic pattern drafting method of Korean men's traditional trousers. We drew the conclusion that Korean men's trousers composed of circles, triangles, and squares express the five elements diagram through their plane structure, and designed in circles with cones and pyramids, and are made to well express the principle of the small cosmos of our body. Moreover, when the basic pattern of Korean men's trousers explained with the antipathy diagram is viewed to create all things while maintaining opposite and complementary relationship with sangsang (mutually benefitting factor), it would be correct to say that the principle of the top verse the bottom is viewed as conflicting. From the structural point of view, since the philosophy of the Samsu principle (the principle of 1,2 and 3 in which 1 represents the heaven, 2 represents the earth, and 3 represents the people) is hidden in Korean men's trousers in which Hurimalgi (the part of trousers corresponding to the waist band of Western pants) is the circle representing the heaven, Marupok is the square representing the earth, and Sapok is the triangle representing the people, we could look at deep meaning of our ancestors expressed in our clothes.

표면 플라즈몬 공명 신호의 증폭을 위한 새로운 금 콜로이드 센서칩 제조와 특성 연구

임정혁,이경호,고광락,강신원,김재호 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

New gold colloidal film sensor chips were employed to enhance the sensitivity of the Surface Plasmon Resonce (SPR). Au collodal solutions were synthesized, and Au colloidal films were fabricated by immobilization of colloid on vacuum deposited Au film through the coupling agent of 1,8-diaminooctane. Specific interaction of human IgG antibody to human antigen was monitored by in situ SPR measurement from both a smooth and a Au colloidal films. Au colloidal film sensor chips resulted in 27 % enhancements effect for the same reaction on SPR angle shifts compared to a smooth Au film.

대학생 성별에 따른 공간 양립성 차이 비교 : 가스레인지 사례를 중심으로

이경태,임정빈 대불대학교 2004 大佛大學校大學院 硏究論文集 Vol.3 No.-

This paper investigated sex difference of the spacial compatibility of the ordinary gas range with four burners and four control switches. Twenty young Koreans and twenty young Chinese participated in the experiment. The statistical difference on the physical arrangement of displays and controls showed betwee the young Koreans and the young Chinese, but no sex difference

이질아메바에 의한 인체 대장상피세포주 HT-29에서의 interleukin-8 유전자의 발현

김정목,정현채,임경일,조양자,김정룡 INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1995 YONSEI REPORTS ON TROPICAL MEDICINE Vol.26 No.1

이질아메바에 의한 장염 환자의 조직 또는 이질아메바를 실험적으로 감염시킨 동물의 조직 검사에서 호중구의 침윤이 특징적으로 관찰된다. 그러나 이와같은 호중구의 침윤을 설명할 수 있는 기전에 대한 연구는 매우 미흡하다. 따라서 본 연구자들은 아메바 감염 초기에 인체 대장상피세포에서 interleukin-8(IL-8)이 유도되어 호중구 침윤과 같은 염증반응이 유발될 것이라는 가설을 설정하였다. 이를 위하여 인체 대장상피세포주인 HT-29에 이질아메바 영양형을 실험적으로 노출시킨 뒤 발현되는 IL-8 mRNA를 역전사 중합효소법(reverse transcriptional polymerase chain reaction, RT-PCR)으로 검사함과 동시에 발현된 IL-8 mRNA를 인공적으로 합성시킨 표준 RNA와 RT-PCR법을 이용하여 정량하였다. 실험 결과 이질아메바 영양형에 노출된 30분 후 부터 IL-8 mRNA가 발현되기 시작하였다. 그리고 그 발현 분자수는 노출 시간의 증가에 따라 계속 증가하여 3시간 대에는 3.1×10(7) molecules/㎍ total RNA를 나타내었다. 동시에 IL-8 mRNA의 발현은 노출시킨 이질아메바 영양형의 수에 비례하였다. 즉, HT-29/아메바 영양형의 비율이 10:1인 경우 IL-8 mRNA의 발현 분자수는 1.2×10(7) molecules/㎍ total RNA로 나타났다. 이와같은 IL-8 mRNA의 발현은 IL-8 단백질 분비로 이어짐을 ELISA 검사로 확인할 수 있었다. 한편 이질아메바 파쇄액(lysate)도 대장상피세포주인 Caco-2에서 IL-8 mRNA발현을 유도하였다. 결론적으로 본 실험은 이질아메바 감염 초기에 대장상피세포로 부터 IL-8이 발현되며 이에 의하여 염증반응이 촉발될 가능성이 있음을 시사해 준다. The protozoan parasite, Entamoeba histolytica, is one of major causative agents of intestinal disease all over the world. In acute experimental infection, the early host response to E. histolytica is characterized by an infiltration of neutrophils. However, the chemotactic signal for this response is not well known. Based on the finding that human epithelial cells produce the potent neutrophil chemoattractant and activator, interlukin-8 (IL-8), IL-8 gene expression was examined thoroughly in human colon epithelial cells exposed to E. histolytica trophozoites. Cellular RNAs were extracted from HT-29 or Caco-2 human colon epithelial cells exposed to E. histolytica trophozoities for 30 minutes. 1 and 3 hours. IL-8 mRNA transcripts were measured by reverse transcriptional polymerase chain reaction (RT-PCR) using synthetic standard RNA. The number of IL-8 mRNA molecules increased from 30 minutes to 3 hours of exposure period, reaching 3.1×10(7) molecules/㎍ of total RNA. Expression pattern of IL-8 mRNA transcripts was parallel to the amounts of IL-8 protein measured by enzyme-linked immunosorbent assay (ELISA). Lysates of E. histolytica also induced expression of mRNA for IL-8 in colon epithelial cells. These results suggest that acute inflammatory reaction by E. hisstolytica may be initially triggered by proinflammatory cytokines such as IL-8 secreted from epithelial cells of the colon.