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- 저자 : 정현채 (검색결과 724 건)
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鄭玹采,윤대현,임화준,김기선 慶熙大學校 1987 論文集 Vol.16 No.-
The ν_6 band of the ethane gas molecule was theoretically synthesized. The intensity and the frequency of each rotational line were computed with VAX-11 computer. The theoretically synthesized spectrum was compared with the experimentally obtained spectrum, which was taken through FT-IR spectrophotometer MX-3600. The R and P branches of the ν_6 band contour of the ethane gas molecule were different from the regular symmetrie top molecule and rather similar to the linear molecules as if there were no K dependence. The absorbance of the P-branch spectrum was overlapped by the unknown branches of the neighbour bands The intensities and frequencies of the unknown lines were not indentified. The overall band contour of the observed spectrum was quite well coincided with the theoretical spectrum except the P-branch.
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정현채,김보배,박현준,이규연,김희훈,심호철,도현진,현창기,도명술 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.5
Metabolic diseases, such as glucose intolerance and nonalcoholic fatty-liver disease (NAFLD), are primary risk factors for life-threatening conditions such as diabetes, heart attack, stroke, and hepatic cancer. Extracts from the tropical tree Moringa oleifera show antidiabetic, antioxidant, anti-inflammatory, and anticancer effects. Fermentation can further improve the safety and nutritional value of certain foods. We investigated the efficacy of fermented M. oleifera extract (FM) against high-fat diet (HFD)-induced glucose intolerance and hepatic lipid accumulation and investigated the underlying mechanisms by analyzing expression of proteins and genes involved in glucose and lipid regulation. C57BL/6 mice were fed with normal chow diet (ND) or HFD supplemented with distilled water (DW, control), nonfermented M. oleifera extract (NFM), or FM for 10 weeks. Although body weights were similar among HFD-fed treatment groups, liver weight was decreased, and glucose tolerance test (GTT) results improved in the FM group compared with DW and NFM groups. Hepatic lipid accumulation was also lower in the FM group, and expressions of genes involved in liver lipid metabolism were upregulated. In addition, HFD-induced endoplasmic reticulum (ER) stress, oxidative stress, and lipotoxicity in quadriceps muscles were decreased by FM. Finally, proinflammatory cytokine mRNA expression was decreased by FM in the liver, epididymal adipose tissue, and quadriceps of HFD-fed mice. FMs may decrease glucose intolerance and NAFLD under HFDinduced obesity by decreasing ER stress, oxidative stress, and inflammation.
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$\lambdaP_{R}$ 프로모터 열린복합체 형성에 미치는 DNA melting 부위 염기서열의 영향
정현채,노정혜 한국미생물학회 1990 미생물학회지 Vol.28 No.1
To examine the effects of sequence variations near the transcriptional start site on the rate of formation of the open complexes at bacteriophage $\lambda P_{R}$ promoter, two mutant promoters were created by site-specific mutagenesis using synthetic oligonucleotides. Mutant I coatains changes at positions -3 and -4 from TT to CC, thus having a 6-bp long G/C stretch between -10 region and transciptional start site (+1). Mutant II has changes at positions -5 and -6 from GG to AA, thereby having a 9-bp long A/T stretch between positions -11 and -3. Selective filter binding assays were performed to measure the rate of formation of the open complexes between the wild-type or two mutant $P_{R}$ promoters on 664 bp fragments and E. coli RNA polymerase at two temperatures. At 37.deg.C, the wild-type and two mutants showed similar rates for the formation of open complex. The second order rate constant $k_{a}$ and $\tau _{int}$, as determined from the .tau.-plot analysis, were $(6.0\pm0.4)\times10^{6}M^{-1}sec^{-1}$ and $11\pm5$sec, respectively. At 18.deg.C, however, the wild-type and two mutant promoters showed differences in the kinetic parameters. k for the wild-type promoter was (2.2$\pm$0.1)\times 10^{6}M^{-1}sec^{-1}$ and $\tau _{int}$ was 76$\pm$sec. Mutant I and II exhibited differences mainly in the rate of isomerization ($\tau_{int,I}=91\pm$10 sec, int,II=34$\pm$ sec), whereas the second order rate constant $k_{a}$ was similar to the wild type value. This result implies that at $18^{\circ}C$, the isomerization rate is determined by both protein conformational change and DNA melting, which are separable kinetically according to the 3-step mechanism of Roe et al.(1984,1985), and that the base changes affected mainly the rate of DNA melting as predicted.lting as predicted.
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정현채,김기선,선경호,남승영 한국태양에너지학회 1990 한국태양에너지학회 학술대회논문집 Vol.- No.-
본 연구소에서 실험 가동하고 있는 태양열 온수기로 부터 얻은 온수를 이용하여 수온이 낮은 3,4,10,11월 중에는 물론 한 겨울철에도 미꾸라지의 최적 성장온도를 유지 시켰던 바 다음과 같은 연구결과를 얻었다.<br/> 가. 전장 3-4㎝이고 체중 2-4g 정도의 미꾸라지는 15-20℃의 최적 조건을 찾았고 25℃이상은 적당치 못함을 미꾸라지 사망으로 확인하였고 수심은 15-20㎝ 내외의 최적조건을 찾았다.<br/> 나. 1-3g 미꾸라지는 서서히 성장하나 4-6g의 미꾸라지는 빠른 성장을 보임을 확인했고 총개체수의 무게 총량은 1개월간에 30-50g 정도로 증가하였다.
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