種實油脂의 貯藏安定性에 關한 硏究

具成子,張晶玉,曺載銑 경희대학교 부설 식량자원개발연구소 1986 硏究論文集 Vol.7 No.-

In order to investigate the storage stability of seed oils, such as perilla oil, sesame oil, rapeseed oil and peanut oil, the oil seeds and seed oils were stored for 40 days at room temperature (20℃-30℃), 4℃ and -20℃, respectively, than the acid value, peroxide value, TBA value and iodine value of the oil and oil extracted from the stored seeds, were determined. The results were as follows; Acid value, peroxide value and TBA value of these oils were increased sharply during storage at room temperature. Among seed oils, perilla oil was least stable and peanut oil was most stable. The oils extracted from stored seeds were more stable than the stored oils, thus the patterns of oxidation of the oils, extracted from stored seeds at room temperature, were same as that of oils, stored at -4℃. Generally, the quality of seed oils could be preserved when the crude seed oils were stored at the temperature below -20℃ and oils seeds at 4℃, respectively.

담도 및 췌장 악성종양에서의 CAl9-9의 진단적 가치에 대한 평가

명승재,최승목,김은옥,최호순,이성구,김명환,민영일,류진숙 大韓消化器病學會 1995 大韓消化器病學會誌 Vol.27 No.3

種實油脂의 貯藏安定性에 關한 硏究

具成子,張晶玉,曺哉銑 慶熙大學校 1985 論文集 Vol.14 No.-

In order to investigate the storage stability of seed oils, such as perilla oil, sesame oil, rapeseed oil and peanut oil, the oil seeds and seed oils were stored for 40 days at room temperature(20°-30℃), 4℃ and -20℃, respectively, than the acid value, peroxide value, TBA value and iodine value of the oil and oil extracted from the stored seeds, were determined. The results were as follows; Acid value, peroxide value and TBA value of these oils were increased sharply during storage at room temperature. Among seed oils, perilla oil was least stable and peanut oil was most stable. The oils extracted from stored seeds were more stable than the stored oils, thus the patterns of oxidation of the oils, extracted from stored seeds at room temperature, were same as that of oils. stored at -4℃. Generally, the quality of seed oils could be preserved when the crude seed oils were stored at the temperature below -20℃ and oils seeds at 4℃, respectively.

컴퓨터 게임과 학업성취도와의 관계 연구

구성옥,맹승렬,유관희 한국컴퓨터교육학회 2005 컴퓨터교육학회 논문지 Vol.8 No.4

澱粉 Gel의 Rheological Properties에 關한 硏究

張晶玉,具成子 慶熙大學校 1984 論文集 Vol.13 No.-

The experiments were carried out to investigate the rheological properties of Acorn, Buckwheat, Mungbean and Cowpea starch gel. The dynamic viscoelasticity and rupture properties of Gels were determined by Rheolograph-Gel and Dynagraph, respectively. The results were as follows: 1. The results, by Z-ray diffractometry showed C-pattern for Acorn starch, others showed B-patterns. 2. According to the study of amylography, the pasting temperature of Buckwheat starch was higher and maximum viscosity of that was extreamely lower than others. 3. The dynamic viscoelasticity of the sample Gels except Buckwheat starch Gel increased with the rise of refrigerated time, but after 48 hours there were no differences of viscoelasticity parameters. 4. The stress-strain curves showed sigmoid shape. Also it was found that the breaking point coincided with the yield point. It was recognized that the rupture strain decreased with the rise of refrigerated time of sample Gels. 5. According to the sample starch Gels, it was recognized that the rheorological properties of small deformation and large deformation were different.

일록춘폐흡충 충체 단백질의 분획별 항원성

정구성,이옥란 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

일록춘폐흡충의 조항원 중 항원단백질의 항원성 차이를 보고자 이 연구를 시도 하였다. 충체 추출물 조항원을 Sephacryl S-300 gel filtration과 DEAE anion exchange chromatography에 의해 각각 조항원을 분리하였다. 이들 분리된 항원은 SDS-PAGE로 단백질 분획상을 구별 하였고, 효소면역 반응(ELISA)에 의하여 항원성을 비교하였다. Gel filtration으로 분리된 S2항원과 chromatography로 분리된 D4 및 D5 항원이 각각의 분리항원중에서 강한 항원성을 나타내었다. 이들은 항원성이 보다 낮은 다른 분리항원에 비해 분자량 8∼9 kDa, 27∼29 kDa, 37∼39 kDa, 50 kDa, 55 kDa 및 60 kDa 분획의 염색강도가 높았고, 이 중 8∼9 kDa를 제외한 나머지 분획들은 immunoblot으로 강한 항원성이 증명 되었다. This study was done to detect the antigenic component from the crude worm extract of Paragonimus iloktsuenensis. Three and five protein fractions, S1∼S3 and D1∼D5 were separated by Sephacryl S-300 gel filtration and by DEAE anion exchange chromatography, respectively form the crude antigen(whole worm extract: PIWA) of P. iloktsuenensis. These fractions were further analyzed by SDS-PAGE(SDS-polyacrylamide gel electrophoresis). The antigenicities of those protein fractions were evaluated by OD (optical density) value of ELISA(enzyme-linked immunosorbent assay), reacted with the serum of the rats infected with P. iloktsuenensis. The results were as follows: 1. The worm extracts(crude antigens) were separated in three fractions, S1, S2 and S3 by Sephacryl S-300 gel filtration. By SDS-PAGE, the characteristic fraction of S1 was 98 kDa, while those of S2 were 20 kDa, 27∼28 kDa, 38 kDa, 50 kDa, 55 kDa and 60 kDa, and of S3 were 12∼13 kDa, 17∼18 kDa and 35∼36 kDa, respectively. The major fraction was of 27∼29 kDa molecular weight among the three fractions. The worm extracts were separated in five fractions, D1, D2, D3, D4 and D5 by DEAE anion exchange chromatography. The characteristic fractions of D1 were 14∼15 kDa and 17∼18 kDa, and of D2 were 12∼13 kDa and 14∼15 kDa, and of D3 were 27∼28 kDa and 35∼36 kDa, and of D4 were 27∼28 kDa, 35∼36 kDa, 38∼39 kDa and 50 kDa, and of D5 were 8∼9 kDa, 27∼29 kDa, 37∼39 kDa, 55 kDa and 60 kDa, respectively. 2. The antigenicity of the fractions, expressed by the intensity of OD value of ELISA. The S2, D4 and D5 revealed stronger antigenicity than those of others. Statistically, antigenicity of S2 antigen (0.98±0.36∼1.42±0.55) was stronger(p<0.001) than that of S3(0.41±0.11∼0.90±0.41) of most low OD values in separated antigens by gel filtration, and D5 antigen (0.73±0.22∼1.38±0.65) of highest OD value was stronger(p<0.001) in all infection. That of D2(0.37±0.13∼0.51±0.23) of lowest OD values in separated antigens by chromatography. D1 showed relatively strong antigenicity compared with the other in periods of two and four weeks after infection. Among separated antigens, the 27∼29 kDa and 37∼39 kDa of protein components fractioned from whole worm extract crude antigen of P. iloktsuenensis plays a important role of humoral immune reaction in this parasitic infection, and the protein of 17∼18 kDa was regarded as sensitive antigen at early period.

신생아 황달질환에서의 RDW의 진단적 의의

김행미,신채옥,현명철,이건수,이상범,구자훈 慶北大學校 醫科大學 1995 慶北醫大誌 Vol.36 No.3

목적 : 신생아 적혈구는 그 모양이 불규칙하여 여러 측정치가 성인 및 소아와 다르다, 저자들은 국내에서 보고된 바 없는 신생아의 Red cell distribution width(RDW)를 조사하여 신생아 정상 측정치를 제시하는 동시에 신생아 시기의 적혈구에 손상을 미치는 질환의 RDW를 분석하여 진단상 의의를 조사하였다. 대상 및 방법 : 신생아 집중치료실에 입원한 만삭아중 감염소견이 없었던 78명과 ABO부적합증 및 패혈증 환아에서 hemoglobin, hematocrit, MV, RDW 및 망상적혈구를 측정하였다. 결과 : 생후 1일부터 7일까지의 만삭신생아의 RDW 17.4±1.5로 소아 정상치 13.4±1.2%에 비해 증가되어 있었으나 출생후 7일까지 hemoglobin, hematocrit는 의의있게 감소한 반면 RDW는 변동이 없었다. 같은 연령의 ABO 부적합증 및 패혈증 환아군은 대조군에 비해 의의있게 hemoglobin과 hematocrit가 낮았으나 RDW는 대조군과 질병군 및 각 질병군 사이에 차이를 보이지 않았다. RDW와 망상적혈구는 ABP 부적합증에서만 상관계수 0.91 (p<0.001)의 상관관계를 보였다. 결론 : 출생후 7일까지 계측한 정상 신생아의 RDW는 소아에 비해 증가되어 있으나 출생후 변화는 없었다. ABO 부적합증 및 패혈증 환아군의 RDW는 대조군과 질병군 및 각 질병군 사이에 차이를 보이지 않아 이들 질환의 진단적 의의가 없었다. ABO 부적합증에서 RDW가 망상적혈구 수와 높은 상관관계를 나타내므로서 자동 분석기로 측정된 RDW로서 망상적혈구 수를 추정할 수 있었다. The RBC distribution width(RDW) has been reported to be of value in the early and differential diagnosis of several RBC disorders, with no sufficient studies on the newborn population. Seventy-eight normal fullterm newborns were studied to establish normal values for RDW of 1st week of life. The RDW of 43 patients with ABO incompatibility or sepsis was then evaluated for the usefulness of the RDW in diagnosis of Jaundice. The RDW was 17.7 ± 1.2 and 17.5 ± 0.8 at 1st and 4-7th days of life, revealing no differences de pending on the postnatal age. The RDW of newborns with ABO incompatibility, sepsis with and without jaundice were 18.4 ± 2.2, 18.2 ± 1.1 and 17.3 ± 0.9 respectively. The RDW was found to be consistently elevated in all these newborn infants-the normal newborns and the newborns with ABO incompatibility or sepsis-when compared with normal older children at our hospital. That reveals a physiologic state of anisocytosis in the newborn, showing no significant differences between these infants. Our results suggest that RDW alone cannot be used as an indicator to distingish between jaundice induced by hemolysis and by other causes. In this study all parameters examined except the reticulocyte counts of ABO incompatibility, which showed, by regression analysis, no correlation with the RDW. High RDW in ABO incompatibility is consistent with high reticulocyte count. One clinical value of the RDW therefore may lie in its capacity for reflecting active erythropioesis in ABO incompatibility. Thus the study has confirmed that red blood cell anisocytosis, as determined by RDW, has no value to differentiate the etiology of jaundice in the newborn period but it seems that RDW plays a role in determining the reticulocyte count in newborns with ABO incompatibility.

Alcaligences에 의해 생산되는 새로운 Hetero-다당류인 Welan Gum과 Xanthan Gum의 Rheology특성에 관한 비교 연구

박혜정,김미옥,구성자 경희대학교 유전공학연구소 1989 遺傳工學論文集 Vol.1 No.-

Alcaligences로부터 추출된 새로운 미생물 다당류인 Welan gum 과 Xanthanonas campetris 의 발효에 의해 얻은 Xanthan gum의 rheology 특성을 비교 검토하였다. 그 결과 Welan gum의 고유점도는 pH7에서 염을 가하지 않는 상태에서는 Xanthan gum 보다 훨씬 낮았으나 0.1 M-KCI 용액중에서는 비슷한 값을 나타냈다. pH, 온도 및 염농도 의존성은 Welan gum 이 더 안정하였으며, 농도 의존성은 두 시료 모두 pseudoplastic 거동을 나타냈다. 또한 Welan gum과 Xanthan gum은 각각 0.056g/dl 및 0.024g/dl 농도 이상에서 entanglement coupling 이 일어났으며, 염종류에 대한 영향은 1 가염(KCl, NaCl), 2가염(MgCl_(2), CaCl_(2)) 및 Urea에 대해 Welan gum 이 더 안정하였다. The polysaccharide produced by Alcaligence, Welgan gum, was rheologically characterized and compared with Xanthan gum, To evaluate the potential function of Welan gum and Xanthan gum, the physical properties of the gums were determined under various solution conditions. Rheological properties of the gums were determined by measuring intrinsic viscosity of the solution in the different pH and salt concentration. At the range of pH 2-11 and salt 0-1.00 M-KCl, the intrinsic viscosity of Welan gum was ranged from 13.65 to 17.90 dl/g and Xanthan gum was ranged from 8.19 to 41.8dl/g. In the absence of salt, the instrinsic viscosity of Welan gum was not affected by increasing pH of the solution, whereas the intrinsic viscosity of Xanthan gum was appeared maximum at the neutral pH, then decreased both acidic and alkalic pH. Intrinsic viscosity of Welan gum was not affected by increasing salt concentration, whereas intrinsec viscosity of Xanthan gum was decreased with increasing salt concentration. The chain stiffness parameter for the Welan gum was 0.01, for Xanthan gum was 0.005. The overlap parameter of Welan gum and Xanthan gum were 0.056 dl/g and 0.024 dl/g, respectively Welan gum and Xanthan gum were showed shear rate dependent or pseudoplastic. The apparent viscosity of Welan gum and Xanthan gum were decreased as the shear rate increased. The yield stress, proportionality constant, and shear index of Welan gum were increased slightly as the concentration of the gum was increased. The apparent viscosity of Welan gum was decreased slightly by increasing temperature, whereas Xanthan gum was decreased In the presence of salts (KCI, NaCl CaCl_(2), MgCl_(2) and urea). Welan gum was not affected and Xanthan gum was affected slightly.

한국인에게 적절한 기관삽관 위치

이승한,최옥경,정구영,정성필 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Endotracheal intubation is one of the important procedures in ED and various fields. Endobronchial intubation can lead to hypoxemia secondary to tension pneumothorax of intubated side and atelectasis of contralateral side. The placement of tube beyond the vocal cords may results in inadvertent extubation, larygeal spasm and aspiration pneumonia. The conformation methods of endotracheal intubation are auscultation of the chest, observation of a vapor trail, oximetry and capnography, and radiologic evaluation. Owen and associates recommended that oral intubation tubes at the upper incisor teeth at the 23cm in men and the 21cm in women of average adult size would have led to proper placement. We studied that whether the proper depth of endotracheal tube in American is appropriate to Korean. We studied adult patients admitted to ED (of both hospitals during March in 1995. Endotracheal tubes in men were positioned at the 23cm and women had positioned at the 21cm at the upper incisor teeth. The distance of the tip of the endotracheal tube to the tracheal carina on the postintubation chest X-ray then determined. Correct placement of intubation was defined with the tip of the tube≥3cm above the carina and below the level of the larynx. Fifty adult patients were enrolled in the study ; 29 men (58%) and 31 women (42%). Mean height and weight were 170±5.2cm, 68±9kg in men and 158±6.5cm, 56±10kg in women. Reasons for intubation were ; respiratory failure(38%), trauma(16%), increased intracramial pressare(16%), drug intoxication(12%), shock(8%), cardiac arrest(7%)and others(3%). The mean distance from the tip to carina on chest X-ray was 4.13±1.5cm in men and 3.45±2.1cm in women. Proper position was 27/29(93%) in men, 14/21(67%) in women or 41/50(82%). this means the placement of endotracheal tube of our patients was positioned deeper than prion study group(97.6%).(p<.05) We recommend that the 22cm tube mark for men and 20cm tube mard for women at upper incisor teeth would have led to proper placement in Korean adult patient.

위암종과 Epstein-Barr 바이러스와의 연관성에 대한 연구

심광용,김호영,김효열,백순구,권상옥,조미연,이창훈,이종인 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Objectives: Epstein-Barr virus (EBV) has been known to be linked to a spectrum of neoplastic conditions, including nasopharyngeal carcinoma, Burkitt's lymphoma, peripheral T-cell lymphoma and Hodgkin's disease. This study aims to investigate the association of EBV with gastric carcinoma in Korea. Methods: Fifty-three cases of gastric adenocarcinoma were studied for evidence of EBV infection by EBV-encoded small RNA (EBER) in situ hybridization (ISH) on the paraffin sections and amplifying the EBV genome encoding envelop glycoprotein (gp220) with polymerase chain reaction (PCR) in fresh gastric cancer specimens. Results: EBER was detected in 7 (13.2%) of 53 gastric carcinomas and all cases were male. In 6 (85.7%) of 7 EBER-positive cases, the tumors were located in the upper and middle part of the stomach. EBER ISH study showed strong positivity in all the tumor cells, but negativity in surrouning lymphocytes, stromal cells and normal gastric mucosa. DNA PCR was positive in 23 (43.4%) of 53 gastric carcinomas, including all of 7 EBER-positive cases. Conclusion: We could observe some association of EBV with gastric carcinoma, and our findings about the charateristics of EBV-positive gastric cancers involving sex, site and histological type are similar to those in Japan. However, EBV-positive rate of gastric cancer is higher than in Japan and lower than in Western countries. Further studies to elucidate oncogenic mechanism of EBV in gastric cancer should be performed.