쪽파[Allium ascalonicum L.] 재배기간 중 Diazinon, Iprodione 및 Chlorfenapyr의 잔류랑 변화

고광용,나은식,김성헌,김상준,장영희,이규승 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.2

In order to know the residual pattern of pesticides and predict to the degradation period until below MRL, we experimented diazinon, iprodinon and chlorfenapyr for shallot. They were the most detected pesticide in shallot by NAQS (National Agricultural product Quality management Service) survey. In this experiment, we sprayed those pesticides 15days before harvest and analyzed 0, 1, 2, 3, 5, 7, 10, 14 day sample to establish logical equation and to calculate DT_(50). During the cultivating period, the residue amount of diazinon was changed from 1.02 mg/kg (0 day) to 0.01 mg/kg (14 day), DT_(50) was 2.19 days, and iprodione was changed from 1.45 mg/kg (0 day) to 0.14 mg/kg (14 day), DT_(50) was 4.15 days, and chlorfenapyr was changed from 1.5 mg/kg (0 day) to 0.01 mg/kg (14 day), DT_(50) was 1.97 days. The DT_(50) of double amount in those pesticides, diazinon was 2.24 days, iprodione was 4.82 days, and chlorfenapyr was 2.24 days, respectively.

학생 이질성의 실태 분석

高光俊 圓光大學校大學院 1996 論文集 Vol.15 No.-

The necessity of this study is that the students' individual learning in front line school education, that is, the problem of induvidualization of learning, remains a long-cherished desire internally and externally. So far, a great many people have directed their concern and efforts toward solving this problem. There are IPI(Individually Prescribed Instruction), Glaser's Class Model, Carroll's School Learning Model, Bloom's Mastery Learning Model, which have contributed to the individualization, and also the Education Development Project (EDP) developed within this country. The individualization of learning still remains unsolved internally and externally. However, the settlement for that is being explored multi-laterally. In order for the individual learning to be developed in school education scene, it is important to confirm and analyze accurately and reasonably the heterogeneity of each student, the prerequisite for the realization of individual learning. Despite the difference in each student's disposition, concern, learning ability, and learning speed, if teachers grasp precisely and individually and teach these things, the problem of individualization is expected to be solved. What matters is to analyze and confirm precisely each student's individual learning feature regarding the learning contents of particular time to be learned by students in the development process of regular teaching-learning. It is impossible for a teacher to have each student learn individually in the class consisting of many students under the circumstances where the possibility of these things being solved is slim. In this respect, this study puts focus on confirmation of the realities of students' heterogeneity on the basis of a new conception system which can confirm students' heterogeneity in a well-founded manner. This study established the following basic objects of study on the basis of the necessity of the above-mentioned study. This study has its basic objects in investigating the conceptual authority of systemized students' learning feature in a new scholary respects and analyzing corroboratively how it is conducive to assaying and confirming the heterogeneity realities of students in teaching-learning scene. In order to attain such basic objects of this study, two study contents were selected. First, investigate the conceptual authority of the confirmation of students' heterogeneity in the position of the study of Human Education. Second, analyze and confirm each student's heterogeneity in the following six aspects. A. Learner's Starting Point of Learning B. Learner's Ability of Learning in Terms of Difficulty C. Learner's Time Spent in Learning D. The number of assignments of collective common learning E. the accomplishment degree of collective common learning assignment F. the selection accuracy of proper learning assignment Two methods, the analysis of related documents and the corroborative analysis of statistics management, are used in this study. This study was conducted in the study and practice room of The Human Education Research Institute Corporation. The second unit of the third-grade and the fifth-grade were the analysis subjected among the learning results of the first term course of the 1995 school year developed in one third-grade learning class and the fifth-grade learning class of elementary school. On the basis of the said study results, a conclusion was made as follows. In the teaching-learning process, the individualization of learning starts from teacher's accurate grasp of students' heterogeneity. It is the students' learning feature that is basic to teachers' confirming each student's heterogeneity. The success or failure of the individualization of learning in school education depends on how exactly the teacher confirms diverse learning feature of each student in the development process of teaching-learning. What is confirmed after this study is that the conceptual system established in accordance with the Study of Education and the ESANET-STL (Education Service Automation Network for School Teaching-Learning) developed for it to be practiced should be applied. Accordingly, it is prospected that the individualization of learning in the school education of this country will be realized if teachers apply this ESANET-STL properly and confirm each student's learning feature accurately.

들깻잎이 재배 및 저장기간중 Procymidone 및 Bifenthrin의 잔류량변화

고광용,이용재,원동준,박혜진,이규승 한국환경농학회 2003 한국환경농학회지 Vol.22 No.1

들깻잎에서 procymidone과 bifenthrin의 경우 안전사용기준인 약제살포 후 5일에 각각의 농도가 38.16, 1.77 mg/kg 이지만 이는 살포 조건과 재배환경에 따른 농약의 초기부착농도가 높은데서 기인한 것이라고 예상되어진다. 앞에서의 결과들로 미루어 볼 때 일반적인 농약의 안전사용기준으로 농약을 처리한다면 최초살포 이후 재배기간에서 5일간 50% 이상 이 분해되고 10일 후에는 90%이상이 분해된다. 또한 상온저장 중 20~40%가 분해되며 수돗물 세척에 의해서 만으로도 50%이상이 제거되므로 조리과정을 감안하지 않더라도 초기살포량의 약 90%는 분해 또는 제거되어 안전사용기준에 의거하여 사용한다면 두 약제 모두 MRL이하로 잔류하게 되어 별다른 문제가 없을 것으로 예상되어진다. 하지만 현행법상 수확 시점에 그 잔류량을 조사하도록 되어 있으므로 깻잎의 경우 재배가 대부분 하우스에서 이루어짐을 감안한다면 두 약제 모두 초기살포시 작물체로서의 부착량이 많을 경우에 수확시점에서의 잔류량이 MRL을 상회할 가능성도 있음을 알 수 있었다. 따라서 이에 따른 약제 사용량의 축소나 저농도 약제의 사용, 살포일수의 조정 등에 관한 연구가 필요하다고 본다. In order to know residual pattern of pesticides and to predict degradation period until below MRL, we experimented with procymidone and bifenthrin for perlua leaf which were the most detected pesticides by NAQS (National Agriculture-product Quality management Service) survey. In this experiment, we sprayed those pesticides 10 days before harvest and analyzed 0, 1, 2, 3, 5, 7, 10 day samples to establish logical equation and to calculate DT_(50). Also degradation patterns of those samples were compared during storage at 4℃ and 20℃. During cultivating period, procymidone residue amount was changed from 79.52 mg/kg (0 day) to 4.2 mg/kg, DT_(50) was 2.65 days by logical-equation, and bifenthrin residue amount was changed from 5.03 mg/kg (0 day) to 0.17 mg/kg, DT_(50) was 2.24 days. During storage period, DTso of procymidone and bifenthrin at 4℃ was 12.23 days and 10.57 days, and at 20℃ was 6.32 days and 8.2 days, respectively.

여고 테니스 우수선수와 비우수선수의 호흡순환기능에 관한 연구

고기준,이재규,김종인,이광무,한재웅,신군수,김태운,안병철,문혜경 釜山大學校 附設 體育科學硏究所 1999 體育科學硏究所 論文集 Vol.15 No.-

The purpose of this study was to evaluate a study on Cardiorespiratory Functions of Elite Tennis Player and Non-Elite Tennis Player in Female High-School. The subjects were included 2 group(each group n=7); Elite Tennis Player group and Non-Elite Tennis Player group. The conclusion of this study was as follows: 1. Elite Tennis Player group showed significantly high in VO2max(p<.001) 2. Elite Tennis Player group showed significantly high in VCO2(p<.001) 3. Elite Tennis Player group showed significantly high in VO2max/kg(p.05)

여고생의 BMI와 체력과의 상관관계

이광무,고기준,한재웅,김태운,김종원 釜山大學校 附設 體育科學硏究所 1998 體育科學硏究所 論文集 Vol.14 No.-

The purpose of this study was to investigate the correlation of BMI and physical fitness in high school girls. The subjects were high school girls(n=295). The measurement items are physical fitness, physique, physique index(BMI : Body Mass Index). The conclusion of this study was as follows; 1. The record of 100M sprint, standing long jump, and sit ups showed a slack downward curve(low record) as BMI rises. 2. The record of softball showed a slack upward curve(high record) as BMI rises. 3. The record of 800M run, sit-ups and softball throw, from the top to the bottom. 4. The order of correlation between BMI and physical fitness was, flexed-arm hang, 100M sprint, standing long jump, 800M run, sit-ups and softball throw, from the top to the bottom. Therefore, we concluded that correlation BMI and physical fitness showed not so high.

物流輸送 手段의 選擇을 위한 知能的 意思決定支援시스템 Prototype

이준석,고광선 한국정보전략학회 1998 한국정보전략학회지 Vol.1 No.1

In these competitve times, there is great pressure to improve customer service, and at the same time to find cost savings wherever possible. Many manufacturers use the private carrier, or the contracted transportation service, for the purpose of collecting and deilvering shipments for their facilities. Transportation is the largest component of logistics cost. Also, few researches have developed this field and they are fragmentary. The advanced in computer technology has resulted in the greatest impact on business since the industrial revolution. The transportation logistics is no exception to this pheomenon. Especially, Intelligent decision support system(IDSS), integrated expert system(ES) and decision support system(DSS), was significant technology in inventory parts. Therefore, this paper developed prototype IDSS for transportation logistics carrier selection. The focus is on the development of a simple, user-friendly tool that can be used effectively by managers to increase the cost-effectiveness of their transportation logestecs. The IDSS was constructed by using the tools of ART-IM expert system shell, CLIPPER, FORTRAN 77, and SLAMSYS simulation language.

임신부 뇨로부터 정제된 인간 상피세포 증식 인자 유사체의 in vitro bioassay 및 특성

박세철,전재현,남정현,권태종,고인영,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

벤조산흡착, 음이온 교환수지, 단일클론항체을 이용한 immunoaffinity chromatography를 통하여 임신부 뇨로부터 천연의 hEGF를 정제하였다. 정제된 hEGF는 μ Bonda C_18 column을 사용한 HPLC 분석을 통하여 4개의 fraction으로 분리가 가능하였으며 western blot과 double immunodiffusion 실험 결과, 각각의 fraction이 hEGF의 특성을 가진 유사체인 것을 알 수 있었다. 또한 hEGF 표준 물질과의 spiking 및 아미노산 분석 등을 통하여 두 번째 fraction이 nhEGF와 동일한 것으로 확인하였다. nhEGF 및 그 유사체의 생물학적 활성 비교를 위하여 NIH 3T3 세포주에서 5'-Brdu incorporation 측정을 위한 labelling 시간, 혈청 농도의 최적 조건을 결정하였다. NIH 3T3 세포주의 DNA 합성능은 0.2% FCS가 포함된 저혈청 배지에서 hEGF가 0.1~10 ng/ml 농도로 첨가하였을 때 증가하는 경향을 나타냈다. HPLC를 통하여 분리된 두번째 hEGF 유사체가 다른 유사체보다도 생물학적인 활성이 우수하였으며, rhEGF 표준물질과의 spiking 및 아미노산 서열 분석등을 통하여 nhEGF로 밝혀졌다. 임신부의 뇨의 hEGF 유사체 함량중 natural hEGF는 46%이었다. Natural human epidermal growth facto (nhEGF) was purified from pregnant human urine by benzoic acid adsorption, DEAE-Sepharose ion exchange, and immunoaffinity chromatography. The purified nhEGF was further separated into four fractions using Bondapak C_18 HPLC system. Following characterization by Western blot and double immunodiffusion, we found that each fraction corresponds to four derivatives of the nhEGF. For biological analysis of nhEGF, we optimized the labeling time and serum concentration for the incorporation of 5-bromo-2'-deoxy-uridine (BrdU), a non-radioactive alternative for [^3H]-thymidine uptake, into NIH 3T3 cells. The DNA synthesis of NIH 3T3 cells was gradually increased at the nhEGF concentrations between 0.1~10 ng/ml in the Dulbecco's Modified Eagles Medium (DMEM) containing 0.2% Fetal calf serum (FCS). When we assayed the biological activity of four fractions, the activity of the second fraction was superior to that of the others.

복숭아의 재배 및 저장기간 중 Procymidone, Chlorpyrifos 및 Cypermethrin의 잔류량 변화

이용재,고광용,원동준,길근환,이규승 한국환경농학회 2003 한국환경농학회지 Vol.22 No.3

복숭아의 생산단계에서 잔류허용 기준과 최종 소비단계에서의 잔류양상 평가자료로 활용하기 위하여 복숭아에서의 procymidone, chlorpyrifos 및 cypermethrin의 포장상태에서와 실온(20°C) 및 저온(4°C) 저장시 잔류양상을 알아보았다. Procymidone의 포장상태에서의 반감기는 표준량 3.1, 배량 3.4일로 나타났고, chlorpyrifos는 포장상태에서 포준량 7.2, 배량 5.8일로 나타났으며, cypermethrin은 표준 10.1, 배량 14.4일로 나타났다. 약제 살포 후 초기 부착량이 MRL 이하로 내려갈 때까지의 시간을 계산해 본 결과 배량으로 살포 할 경우 procymidone과 chlorpyrifos는 안전사용기준 보다 시간이 오래 걸렸지만, 표준량으로 사용할 경우 별다른 문제가 없는 것으로 나타났다. 저장기간 중의 procymidone, chlorpyrifos, cypermethrin의 반감일수는 20°C에서 4.6, 10.2, 12.9일 이며, 4°C에서는 16.1, 14.3, 13.1일로 저온에서의 반감기가 실온에서보다 더 긴 것을 알 수 있었다. 한편 세척방법에 의한 잔류농약의 제거율은 수돗물에서는 최고 procymidone 82.9%, chlorpyrifos 27.0%, cypermethrin 24.0%로 나타났고, 세제를 사용 할 경우 procymidone 88.8%, chlorpyrifos 59.0%, cypermethrin 59.4%의 제거율을 보였다. The residue patterns of procymidone, chlorpyrifos and cypermethrin in peaches were examined. The pesticides were sprayed at 15 days before harvest and then were determined the residue at 0, 1, 2, 3, 5, 7, 10 and 15 days after pesticide application and calculated their DT_(50). Also, the degradation patterns at 4℃ and 20℃ during storage period were compared. Biological half-lives of procymidone, chlorpyrifos and cypermethrin in peaches during the cultivation period were 3.1, 7.2 and 10.4 days, respectively. The biological half-life of procymidone was shorter than the others. During the storage period, half-lives of procymidone, chlorpyrifos and cypermethrin were 16.0, 14.3 and 13.1 days at 4 C and 4.6, 10.2 and 12.9 days at 20℃, resp ectively. The degradation rates of these three pesticides in storage period were slower than them in cultivation period. Removal rates were 22.2--82.9% by tap water, and 12.5∼88.8% by detergent solution.

규칙적인 유산소성 운동이 비만아동들의 신체구성 및 호흡순환기능에 미치는 영향

김태운,신군수,김종인,이광무,김성헌,안병철,고기준,한재웅,이재규 釜山大學校 附設 體育科學硏究所 1997 體育科學硏究所 論文集 Vol.13 No.-

The purpose of this study was to examined the effect of supervised regulary aerobic exercise program with cardiorespiratory function of obese children. The subjects were 16 obese Childrens(elementary school 4∼5 grade), and engaged in regulary aerobic exercise program for 9 weeks(5 days/week, 100 min/day, 60∼70% HRmax) with mountain climbing, basketball and football. The items of cardiorespiratory function measured VEmax, HRmax, RQmax, VCO₂max, VCO₂max, VCO₂max/kg and BFmax after treadmill exercise load. The conclusion of this study was as follows ; 1. In body composition, there were significant decrease(P<0.001) after aerobic exercise for 9 weeks in body weight, percent body mass index 2. In cardiorespiratory function, there were significant increase(P<0.001) after aerobic exercise for 9 weeks in VEmax and VCO₂max by treadmill exercise load. 3. In cardiorespiratory function, there were significant increase(P<0.001) after aerobic exercise for 9 weeks in VO₂mex and RQmax by treadmill exercise load. 4. In cardiorespiratory function, there were significant increase(P<0.001, P< 0.01) after aerobic exercise for 9 weeks in VO₂max/kg and BFmax by treadmill exercise load.

여중생들의 신체조성과 심폐기능 및 심박수 회복율에 관한 비교 연구 : 비만 여중생과 정상 여중생을 중심으로

김태운,신군수,김종인,이광무,김성헌,안병철,고기준,한재웅,이재규 釜山大學校 附設 體育科學硏究所 1996 體育科學硏究所 論文集 Vol.12 No.-

The purpose of this study was to compare and analyze the relation between body composition, cardiorespiratory functions and percent heart rate recovery in obese and normal middle school female students. We used total of 12 middle school female students(3rd grade) as subject and devided them into two groups : The measurement items of cardiorespiratory functions were HR, VE, RQ, VO₂, VCO₂, VO₂/kg, O₂pulse, VE/VO₂, VE/VCO₂, before and after treadmill exercise load. The statistical analysis were M±SD and t-test for groups(α=.05) using ?? statistical package. The conclusion of this study was as follows ; 1. In 5 items(FM, LBM, %Fat, TBW, BMI) all of body composition, obese group has been significantly higher than normal group(P<0.01). 2. In heart rate and VO₂ of resting(before exercise loads) cardiorespiratory functions, normal group has been significantly higher than obese group(P<0.05). 3. In VEmax of cardiorespiratory functions after exercise loads, obese group has been significantly higher than normal group(P<0.05), and in VO₂max, obese group has been significantly higher than normal group(P<0.01). 4. In 1, 3, 5 minute percent recovery all of percent heart rate recovery after exercise loads, normal group has been higher than obese group but there was no statistical significant difference.