영상분석 시스템 구현(Ⅰ)

김동욱,한한수,김석경,이중,하영호,김희수 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

RESEARCH ARTICLE : Amniotic membrane extract-loaded double-layered wound dressing: evaluation of gel properties and wound healing

( Yeung Keun Choi ),( Fakhar Ud Din ),( Dong Wuk Kim ),( Yong Ll Kim ),( Jong Oh Kim ),( Sae Kwang Ku ),( Jeong Chan Ra ),( Jae Wook Huh ),( Jangik L Lee ),( Dong Hwan Sohn ),( Chul Soon Yong ),( Han 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

The conservative single-layered wound dressing system is decomposed when mixed in polyvinyl alcohol (PVA) solution, which means it cannot be used with a temperature-sensitive drug. The goal of this investigation was to make an amniotic membrane extract (AME)-loaded double-layered wound dressing with an improved healing result compared to the conservative single-layered wound dressing systems. The double-layered wound dressing was developed with PVA/sodium alginate using a freeze-melting technique; one layer was PVA layer and the other was the drug-loaded sodium alginate layer. Its gel properties were assessed compared to single-layered wound dressings. Moreover, in vivo wound-healing effects and histopathology were calculated compared to commercial products. The double-layered wound dressing gave a similar gel fraction and Young`s module as single-layered wound bandages developed with only PVA, and a similar inflammation ability and WVTR as single-layered wound dressings developed with PVA and sodium alginate. Our data indicate that these double-layered wound bandages were just as swellable, but more elastic and stronger than single-layered wound dressings comprised of the same polymers and quantities, possibly giving an acceptable level of moisture and accumulation of exudates in the wound zone. Compared to the commercial product, the double-layered wound dressing comprising 6.7% PVA, 0.5% sodium alginate and 0.01% AME significantly enhanced the wound-healing effect in the wound-healing test. Histological investigations showed that superior full-thickness wound-healing effects compared to the commercial product. Therefore, the double-layered wound dressing would be an outstanding wound-dressing system with improved wound healing and good gel property.

붕장어 그물통발의 혼획 방지기구 개발에 관한 기초적 연구

김욱성 ( Wook Sung Kim ),이주희 ( Ju Hee Lee ),권병국 ( Byeong Guk Kwon ),유제범 ( Jae Bum Yoo ),김부영 ( Bu Yeung Kim ),김병수 ( Byung Soo Kim ),이혜옥 ( Hye Ok Lee ),조영복 ( Young Bok Cho ) 한국어업기술학회 2006 수산해양기술연구 Vol.42 No.1

기능성 지질 추출 소재로서 Fish Frames의 지질성분 특성

김정균(Jeong-Gyun Kim),한병욱(Byung-Wook Han),김혜숙(Hye-Sook Kim),박찬호(Chan-Ho Park),정인권(In-Kwon Chung),최영준(Yeung Joon Choi),김진수(Jin-Soo Kim),허민수(Min-Soo Heu) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.3

수산가공 부산물인 fish frame을 기능성 지질 추출소재로서의 이용 가능성을 검토하기 위하여 6종의 fish frame(붉은 메기, 연어, 삼치, 고등어, 붕장어, 가다랑어 등)에 대한 지질 성분 특성에 대하여 살펴보았다. Fish frame에 대한 어류 뼈의 수율은 가다랑어가 64.2%로 가장 높았고, 다음으로 연어(57.9%), 붕장어(54.6%), 붉은 메기(42.0%), 삼치(41.7%)등의 순이었으며, 고등어가 32.6%로 가장 낮았다. 어류 뼈 유래 총지질 중 중성지질의 함량은 삼치가 23.3 g/100 g으로 가장 많았고, 다음으로 붕장어(21.5 g/100 g), 연어(16.0 g/100 g), 고등어(15.5 g/100 g)의 순이었으며, 가다랑어와 붉은 메기의 경우 다른 4종의 어류에 비하여 확연히 낮았다. 붉은 메기를 제외한 5종 어류의 중성지질 모두가 근육 및 뼈 지방에 관계없이 TG가 주성분(근육지방, 71.9~83.2%; 뼈지방, 74.2~86.9%)이었고, 다음으로 근육지방의 경우, FFA(8.1~19.2%), 뼈지방의 경우 FS(5.5~15.5%)이었다. 붉은 메기의 경우, 중성지질의 주성분인 TG의 조성비(근육지방, 48.6%; 뼈지방, 45.3%)가 다른 어류에 비하여 확연히 낮았고, FFA의 조성비 또한 근육지방(41.6%) 및 뼈지방 (43.4%)에 관계없이 모두 5종의 어류에 비하여 확연히 높았다. 어류 뼈의 중성지질의 EPA+DHA 조성은 연어가 29.3%로 가장 높았고, 다음으로 가다랑어(27.1%), 붉은 메기(27.0%), 고등어(25.7%), 삼치(21.6%)의 순이었고, 붕장어의 경우 14.9%에 불과하였다. 이상의 수율, 중성지질 함량, 유리지방산 조성 및 EPA+DHA 조성 비율의 결과로 미루어 보아 기능성 지질 추출원으로는 연어가 가장 우수하였고, 다음으로 삼치, 붕장어, 고등어, 가다랑어 등의 순이었으며, 붉은 메기의 경우 부적절하다고 판단되었다. Lipid characteristics of 6 species of fish frame (armored weasel-fish, AW; chum salmon, CS; spanish mackerel, SM; common mackerel, CM; conger eel, CE and skipjack tuna, ST) were studied by determining total lipid (TL) contents, lipid classes, and fatty acid composition. The highest yield of bone was obtained from ST frame (64.2%), followed by CS frame (57.9%), CE frame (54.6%), AW frame (41.6%), SM frame (41.7%), and CM frame (32.6%). The highest neutral lipid content was also found in total lipid (TL) from SM bone (23.3 g/100 g), followed by TL from CE bone (21.5 g/100 g), CS bone (16.0 g/100 g), and CM bone (15.5 g/100 g), while those from ST and AW bones were 7.2 g/100 g and 0.4 g/100 g, respectively. The prominent lipid classes of neutral lipids (NL) from all fish bones and muscles were triglyceride (TG), however, which was much lower in AW than in other fishes. The percentages of EPA and DHA in NL from fish bone were in the descending order of CS (29.3%), ST (27.1%), AW (27.0%), CM (25.7%), SM (21.6%), and CE (14.9%). Based on the lipid characteristics, the CS frame was the best resource for extraction of a functional lipid.

Populus alba × P. glandulosa (은수원사시나무)에 대한 速生長의 이질 3배체 육성연구 Ⅱ. 엽육조직의 원형질체 분리와 microcolony 형성에 미치는 요인

김정석(Chung Suk Kim),김용욱(Yong Wook Kim),김영두(Yeung Du Kim) 한국육종학회 1988 한국육종학회지 Vol.20 No.4

The isolation and culture of protoplasts in mesophyll tissues were conducted to induce further somatic cell hybrids in Populus aiba×P. glandulosa F₁. The results obtained were as follows: Optimum enzymes and its concentrations for mesophyll protoplast isolation and viability were 2.0% cellulase, 1.0% macerozyme and 1.2% hemicellulase, and four-hour incubation showed 5.3×10⁶ protoplasts/g mesophyll which were the best. The 6M of mannitol was the most effective cocentration to protoplast isolation and viability. The 3.4×10⁶ protoplats/g mesophyll were isolated with its viability of 96.5%. Media containing 10mg/ℓ of gibberellin were more effective to preplasmolysis than those containing 30 mM of cystain, and showed 175% in protoplast isolation. the incubation at 4℃ for 24 hours was the most adequate condition. Modified MS medium was the most effective to budding, first cell division and microcolony formation among the media. Among the treatments with various hormones added to liquid media, the treatment of 2.0mg/ℓ NAA, 1.0mg/ℓ IAA, 0.2mg/ℓ 2,4-D and 0.5mg/ℓ BAP showed the lowest cell division and microcolony formation. The medium added with 0.2mg/ℓ 2,4-D only was effective to microcolony formation.

LOC Die Bonder의 접합 공정 해석 및 접합력 제어

김재훈(Jae-Hun Kim),홍성욱(Seong-Wook Hong),김영식(Yeung-Shik Kim),김원남(Won-Nam Kim) 한국정밀공학회 1997 한국정밀공학회 학술발표대회 논문집 Vol.1997 No.5월

PSL, ESR 및 TL 측정에 의한 복합조미식품의 방사선 조사여부 검지

권중호 ( Joong Ho Kwon ),김미영 ( Mi Yeung Kim ),김병근 ( Byeong Keun Kim ),정형욱 ( Hyung Wook Chung ),김태철 ( Tae Cheol Kim ),김수진 ( Soo Jin Kim ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2006 한국식품저장유통학회지 Vol.13 No.1

Methamphetamine 중독증상을 보인 환자에서 발생한 횡문근 융해증 및 급성 신부전 1예

권태경,문봄내,최동진,윤기현,김태균,김경욱,김철홍,김민호,이성주,김영훈 인제대학교 백병원 2001 仁濟醫學 Vol.22 No.2

Drugs are important causes of rhabdomyolysis. In Korea, however, there is no report of rhabdomyolysis developed by methamphetamine intoxication. We experienced a case of rhabdomyolysis developed in a methamphetamine poisoning patient. The patient was a 25-year-old man presenting with altered mental status. Results of further investigations showed evidence of acute renal failure and rhabdomyolysis. He was treated with general supportive care including hydration, urine alkalization and hemodialysis, but this patient died eventually. This case shows the importance of the suspiction of illicit drugs including methamphetamine as a cause of rhabdomyolysis with an unclear etiology.

Helicobacter pylori 감염의 유병률과 관련요인에 관한 연구

김영욱,이수일,조병만,고광욱,김영실,강수용,차애리,김돈균,Kim, Yeung-Wook,Lee, Su-Ill,Cho, Byung-Mann,Koh, Kwang-Wook,Kim, Young-Sil,Kang, Su-Yong,Cha, Oae-Ri,Kim, Don-Kyoun 대한예방의학회 1996 Journal of Preventive Medicine and Public Health Vol.29 No.3

Helicobacter pylori 감염은 만성 위염과 소화성 궤양의 원인인자로 작용하며 위암과도 강한 상관관계가 있는 것으로 증명되어 기존의 위 십이지장 질환의 치료와 관리에 많은 변화를 가져오고 있다. 저자들은 Helicobacter pylori 감염의 유병률을 측정하고 유병상태와 관련되는 요인을 조사할 목적으로 부산광역시와 경상남도에 거주하는 소아 몇 청년 552명을 대상으로 1996년 4월부터 6월에 걸쳐 본 연구를 시행했다. 연구 대상자들의 혈청에 존재하는 Helicobacter pylori Ig G 항체는 Enzyme Linked Immunosorbent Assay(ELISA) 방법으로 측정했으며 설문조사를 통해 기본적인 인구학적 특성, 유병상태와 관련되는 요인에 관한 정보를 얻었다. 연구 대상자들의 총 Helicobacter pylori 감염률은 25.7%였고 $1\sim3$세군에서의 5.8%에서 $20\sim29$세군의 44.4%까지 연령이 증가함에 따라 유병률도 비례해 증가하는 양상을 보였다($\chi^2$ for trend=57.9, p<0.0001). 특히 학동기 전 연령인 $4\sim6$세 군에서 초등학교 1, 2, 3학년 연령인 $7\sim9$세 군간에 유병률의 급격한 상승이 관찰되어 이 시기에 Helicobacter pylori 감염이 본격적으로 발생하는 것으로 추측된다. 다변량 로지스틱 회귀 모델에서 연령, 가족수 및 거주하는 주택의 형태가 유병상태와 유의한 상관관계를 보였으며 각각의 교차비(95% 신뢰구간)는 연령 $1\sim9$세를 기준으로 $10\sim19$세 $3.6(2.0\sim6.4)$, $20\sim29$세 $7.3(4.1\sim13.1)$ 였고, 가족수 $1\sim3$명을 기준으로 $4\sim5$명 $2.1(1.3\sim5.4)$, 6명 이상 $2.7(1.3\sim5.4)$였으며 주택의 형태는 아파트 거주를 기준으로 일반주택 및 연립주택 $1.9(1.1\sim3.5)$ 이었다. Helicobacter pylori is now recognized as causative agent of chronic gastritis and peptic ulcer, and strongly associated with development of gastric carcinoma. With development of sensitive and specific serologic tests to identify individuals infected with Helicobacter pylori, the epidemiologic study of this diseases has been investigated. But it's transmission route is not established, yet. The purpose of this study is to measure the prevalence of Helicobacter pylori infection in healthy children and young adults and to evaluate related factors for Helicobacter pylori infection in Korea. The seroprevalence of Ig G antibodies to Helicobacter pylori was determined using a Enzyme Linked Immunosorbent Assay and we obtained the information, such as demographic characteristics, monthly household income, numbers of family members in the house, numbers of persons using same room, type of house, and type of drinking water through the questionnaire survey. The observed overall seropositivity rate was 25.7%. The rate is increased progressively from 5.8% in the age group $1\sim3$ years to 44.4% in the age group $20\sim29$years($\chi^2$ for trend, p<0.001). Especially, the rate increased steeply from 6.5% in the age group $4\sim6$ years to 20.8% in the age group $7\sim9$ years, and this suggested that elementary school age was the major acquisition time of Helicobacter pylori infection. In multivariate logistic regression model, age, numbers of family members in the house, and type of house was statistically significant variables for Helicobacter pylori infection. Each odds ratio(93% CI) were as follows; base to age group $1\sim9$ years, age group $10\sim19$ years $3.6(2.0\sim6.4)$, age group $20\sim29$ years $7.3(4.1\sim13.1)$ and base to group of $1\sim3$ family members, group of $4\sim5$ family members $2.1(1.1\sim4.0)$, group of 6 or more family members $2.7(1.3\sim5.4)$ and base to apartment, single and multihouse $1.9(1.1\sim3.5)$. Sex, monthly household income, numbers of persons using same room, and type of drinking water was not statistically significant for Helicobacter pylori infection.

일부 농촌지역 노인들의 인지 장애에 관련된 요인에 관한 연구

고광욱,조병만,이수일,김돈균,조봉수,김영욱,김영실,강수용,Koh, Kwang-Wook,Cho, Byung-Mann,Lee, Su-Ill,Kim, Don-Kyoun,Cho, Bong-Su,Kim, Yeung-Wook,Kim, Young-Sil,Kang, Su-Yong 대한예방의학회 1996 예방의학회지 Vol.29 No.3

경상남도 일부 지역에서 60세 이상 노인들의 MMSE에 의한 인지 장애 유병률을 조사하고 인지 장애에 영향을 미치는 요인들을 조사하여 다음과 같은 성적을 얻었다. 1. MMSE에 의한 인지 장애의 유병률은 35.9%로 남자에서 18.4%, 여자에서 45.2%였다. 2. 인지 장애 유병률은 여자에서 유의하게 높았고(p=0.02) 무학력자와 문맹자의 인지 장애 유병률이 통계적으로 유의하게 높았다(p=0.03). 3.성별과 교육 수준에 따른 층화 분석에서 알루미늄의 인지 기능에 대한 독성 효과와 칼슘의 보호 효과에 대한 전체적으로 일정한 경향은 관찰되지 않았다(p>0.05). 4. 최종 분석 대상자 전체에서 음용수 중의 알루미늄과 인지 기능 검사 점수간의 상관 계수는 -0.066으로서 미약한 음의 상관관계에 있었으나 통계학적인 유의성은 보이지 않았고(p=0.434), 칼슘과 MMSE 점수와의 관계에서도 기대했던 바의 양의 상관관계는 관찰할 수 없었다. To investigate the factors which affecting the cognitive impairment of the 60 or more age group, the authors surveyed for the subjects in some area of Kyungnam Province. 201 studied subjects were tested for cognitive function with mini-mental state examination(MMSE). Information on demographic characteristics and life style has been collected through direct interview. The concentration of Ai and Ca of subject's drinking water, which might be related with cognition, was measured by Inductively Coupled Argon Plasma Spectrometer. The main results were summarized as follows. 1. The prevalence rate of cognitive impairment was 18.4% in male and 45.2% in female and this sexual difference was statistically significant(p=0.03). And the uneducated or illiterated showed significantly high prevalence rate of cognitive impairment(p=0.02). 2. In stratified analysis by sex md education year, we can not see significant trend indicating the neurotoxic effects of aluminum and protective effects of calcium to the cognitive function(p>0.05). 3. The correlation between the concentration of aluminum in drinking water and the MMSE score in whole subjects showed weak negative relationship(r=-0.066). But there was no statistical significance(p=0.434).