약물의 in vitro 투과 실험을 위한 사람의 비강점막상피세포 단층막의 일차배양

유진욱,김유선,이민기,노환중,이치호,김대덕 한국약제학회 2002 Journal of Pharmaceutical Investigation Vol.32 No.1

The primary culture of human nasal epithelial cell monolayer was performed on a Transwell. The effect of various factors on the tight junction formation was observed in order to develop an in vitro experimental system for nasal transport studies. Human nasal epithelial cells, collected from human normal inferior turbinates, were plated onto diverse inserts. After 4 days, media of the apical surface was removed for air-liquid interface (ALI) culture. Morphological characteristics was observed by transmission electron microscopy (TEM). A polyester membrane of 0.4 μm pore size was determined as the most effective insert based on the change in the transepithelial electric resistance (TEER) value as well as the ^14C-mannitol transport study. The ALI method was effective in developing the tight junction as observed in the further increase in the TEER value and reduction in the permeability coefficient (P_app) of ^14C-mannitol transport. Results of the transport study of a model drug, budesonide, showed that the primary culture system developed in this study could be further developed and applied for in vitro nasal transport studies.

화장품 유통경로에 따른 소비자 쇼핑성향, 정보원, 점포이미지에 관한 연구

선정희,유태순 한국의류학회 2004 한국의류학회지 Vol.28 No.5

The present study attempts to identify the differences in consumer information source, shopping orientation, and store image in the purchase of cosmetics in adult women at the age of 20 or more according to the distributions channels: department stores, specialty stores, discount stores and direct mails. From October 3 to 13, a survey was conducted to 1,200 adult women who visited each channel and who would understand the purpose of this study and complete the questionnaire. 300 sheets were distributed to each of the channels under investigation, and a total of 1,116 questionaaires were used in the final analysis. Using the statistical package, SPSS WIN 10.0, factor analysis, ANOVA, and Duncan's post-hoc test were carried out. The result are as follows: 1. There was a difference in the demographical characteristics of the consumers according to the distribution channels for cosmetics. 2. There was a difference in the shopping orientations of the consumers according to the distribution channels for cosmetics. 3. There was a difference in the information sources of the consumers according to the distribution channels for cosmetics. 4. There was a difference in the store images perceived by the consumers according to the distribution channels for cosmetics.

중이기형을 동반한 안이척수이형성 증후군에서 시행한 난원창 개창술 1예

조양선,정유석,이종찬,추광철 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.12

고령토로부터 알루미나 추출에 관한 연구(Ⅱ) : 질산에 의한 추출

유대선,이희수,김정한 연세대학교 산업기술연구소 1990 논문집 Vol.22 No.2

The preparation conditions of alumina powder extracted from domestic San-Cheong Kaolin by nitric acid were investigated. The experimental conditions which have an effect on the yield of alumina were as follows ; the calcination temperature and time, the acid concentration, the reaction temperature and time, pH of the extracted solution, and particle size of the raw material. The aggolomeration of pseudo-boehmite precipitated by NH₄OH at pH 8 was controlled by various drying methods. α-Al₂O₃powder prepared by heating of the dried powder for 1 hour at 1200℃. Under the optimum condition, the yield and purity of α-Al₂O₃powder were 73.6 percent and 95.5 percent, respectively. The alumina particles prepared by the freeze drying method revealed narrower size distribution and less agglomerate than others. And impurities of the raw material which have an effect on the extraction of alumina were Kyanite and Anorthite.

전류 비유 모델에 의한 초등학생의 전류 개념 변화 분석

유병길,강인석,김병철,남만희,박선희,소하연,윤희정,이영아,이하룡,전병문,정승호 부산교육대학교 과학교육연구소 2000 科學敎育硏究 Vol.25 No.-

There exists a major impediment to learning electricity which may illuminate why direct presentation of scientists' ideas is too aggressive a strategy. This impediment lies in thinking that electricity is used up and in envisaging the light bulb as a consumer. This view is based on a powerful idea which summarises much of our experience; it declares that 'things get used up', 'you cannot get something for nothing', and 'intuitive thinking'. To replace this view with scientific view, we made the analogy model. It is suggested that the analogy model is more effective in teaching electricity circuits than conventional teaching method.

도서관리 종합 정보 시스템 설계 및 구현

윤선심,김영준,유재중,이호근,김영현,임한수,박두순 순천향대학교 부설 산업기술연구소 2002 순천향 산업기술연구소논문집 Vol.8 No.1

There has been a sizable growth in the use of rich media over the World Wide Web(WWW) as more individuals and institutions realize rich-media's potential in a broad range of applications, including electronic commerce, education and training, news, etc. Internet and WWW technologies provide the information of the web. Libraries are systems that carry out interactions among information and knowledge seekers. Library become more and more important for the provision of data. Total information system for books management exist in many forms. We present total information system for books management which is applied to several different sources, e.g., administrator security login, statistics about the present state of lending, printing management, and convenient GUI(Graphic User Interface) environment.

임플란트의 collar design이 변연골과 연조직에 미치는 영향

유현상,강선녀,정창모,윤미정,허중보,전영찬 대한치과보철학회 2012 대한치과보철학회지 Vol.50 No.1

연구 목적: 임플란트 경부가 잘 설계된 경우 양호한 연조직 반응을 통해 변연골을 보존하는데 도움이 된다. 본 실험에서는 연, 경조직 경계부에 가장 가까이 위치하는 임플란트의 collar design이 변연골 변화와 연조직 반응에 미치는 영향을 동물실험을 통해 알아보고자 하였다. 연구 재료 및 방법: 2마리의 건강한 Beagle dog에 임플란트 collar design만 다른 두 종류의 임플란트(Neobiotech Co. Seoul, Korea)를 식립하였다. Collar에 bevel 을 부여한 군(Bevel 군)과 "S"자 형태를 부여한 군(Bioseal 군)으로 나누어 마리 당 7개, 군당 7개, 총 14개의 임플란트를 무작위로 식립한 후 Healing abutment를 즉시 체결하였다. 디지털 표준구내 방사선사진을 이용해 4주 간격으로 총 12주간 근원심 변연골 변화를 관찰하였고, 12주에 희생하여 조직학적 분석을 통해 협설 변연골 흡수 및 임플란트 주변 연조직 반응을 평가하였다. Mann-Whitney test를 통해 동일한 방사선 사진 촬영 시점에서 근원심 변연골 변화량 및 조직계측치를 군 간 비교하였고, Kruskal-Wallis test를 통해 방사선 사진상 근원심 변연골 변화량이 시간에 따른 차이가 있는지 군 내 분석 한 후 Duncan test를 통해 사후 검증하였다(α=.05). 결과: 방사선학적 분석 결과 각 촬영 시점에서 두 군간 근원심 변연골 변화량의 차이를 보이지 않았다(P>.05). 군 내에서 시간에 따른 근원심 변연골의 흡수량을 분석한 결과 Bevel 군에서는 시간에 따른 차이를 보이지 않았으나 (P>.05), Bioseal 군에서는 시간에 따른 차이를 보였으며, 4주및8주와 비교했을 때 12주에서 변연골의 증가를 보였다(P<.05). 조직학적 분석 결과 협설측 변연골 흡수량에서 두 군간 차이를 보이지 않았으나 (P>.05), Bevel 군에 비해 Bioseal 군에서 더 견고한 결합조직부착을 관찰할 수 있었으며, 생물학적 폭경의 값은 두 집단 간 차이를 보이지 않은 반면에 (P>.05), 접합상피부착은 Bevel 군에서 유의하게 길었고, 결합조직부착은 Bioseal 군에서 더 길게 나타났다(P<.05). 결론: Bevel 군에 비해 Bioseal 군에서 결합조직부착은 길게 형성된 반면에 접합상피부착은 더 짧게 나타났으며, 생물학적 폭경과 초기 변연골 흡수에는 차이가 없음을 알 수 있었다. 연조직 반응의 차이가 실제 기능하중 하에서 변연골 변화에 미치는 영향에 대해서 향후 연구가 필요할 것으로 생각된다. PURPOSE. The purpose of this study was to investigate the effects of implant collar design on marginal bone change and soft tissue response by an animal test. MATERIALS AND METHODS. Two types of Implant (Neobiotech Co. Seoul, Korea) that only differs in collar design were planted on two healthy Beagle dogs. The implants were divided into two groups, the first group with a beveled collar (Bevel Group) and the second group with "S" shaped collar (Bioseal group). Standardized intraoral radiographs were used to investigate the mesio-distal change of the marginal bone. Histological analysis was done to evaluate the bucco-lingual marginal bone resorption and the soft tissue response adjacent to the implant. Mann-Whitney test was done to compare the mesio-distal marginal bone change at equivalent time for taking the radiographs and the tissue measurements between the groups. RESULTS. Radiographic and histological analysis showed that there was no difference in marginal bone change between the two groups (P>.05). Histological analysis showed Bioseal group had more rigid connective tissue attachment than the Bevel group. There was no difference in biological width (P>.05). Bevel group showed significantly longer junctional epithelium attachment and Bioseal group showed longer connective tissue attachment (P<.05). CONCLUSION. For three months there were no differences in marginal bone change between the Bevel group and the Bioseal group. As for the soft tissue adjacent to the implant, Bioseal group showed longer connective tissue attachment while showing shorter junctional epithelium attachment. There were no differences in biologic width.

개에서 발생한 신우신염 증례

유건우,이선희,임수정,박형진,김태신,송은식,송근호 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2009 動物醫科學硏究誌 Vol.16 No.1

A 6 years old Schunauzer dog (7.2kg, intact female) with history of hematuria for two days was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. Complete blood cell count showed no significant abnormalities and serum chemistry profile showed increased phosphate profile. On urinanalysis, there was evidence of bacterial infection. On ultrasonographic evaluation, irregular renal contour and increased echogenecity, pelvic and uretral dilation, and thickening of urinary bladder wall were observed. Based on these finding, the dog was diagnosed as pyelonephritis. The dog was treated with antibiotics based on antimicrobial susceptibility test results, then successfully resolved after 8 weeks of treatment.

서울 및 경기지역 노인의 신체적 건강 및 기능적 건강 실태 조사

최윤정;박유신;이상선 한양대학교 2010 韓國 生活 科學 硏究 Vol.30 No.2

This study was performed to assess anthropometric status, functional health and physical fitness among the elderly in Seoul and Kyunggi-do. Four hundred forty free-living elderly people(127 male, 331 female), aged 65 or more were interviewed by trained interviewers using structured questionnaires including demographic information, activities of daily living(ADL), instrumental activities of daily living(IADL), physical activity. And they measured anthropometic status and physical fitness. All the data were analyzed by student t-test, chi-square analysis using SPSS 9.0 version at p<0.05. The results can be summarized as follow : Of all the subjects, 46.3% were obesity, especially 92.4% of female were abdominal obesity. In functional health status, ADL score was 6.62±1.54, and IADL score was 25.94±6.92. Most of the subjects were difficult to walk and run. Female was a significantly higher flexibility and body fat than male(p<0.001), whereas female was a significantly lower muscular endurance than male(p<0.001).

아로마 요법이 뇌졸중환자의 자율신경계와 스트레스에 대한 신체 저항도에 미치는 효과

신유선,조영숙,정유진 대한기초간호자연과학회 2004 Journal of korean biological nursing science Vol.6 No.2

Purpose : The purpose of this study is to determine effects of aromatherapy on the activity and balance of autonomic nervous system and on physical resistance to stress. Methods : This research is a primitive experimental design which attempts to analyze aromatherapy EKG monitoring on real-time basis. Lavender & Chamomile, each 30 drops, were blended with 100ml Sweet Almond oil. Subjects was laid relaxed, and had insides of his wrists and ankles attached with electrodes for EKG. Right after applied with essential oil onto his philtrum and parotid, started on EKG monitoring. Results : The activities of parasympathetic and sympathetic nerve were changed as time elapsed, with significant differences(p=.000). In multiple comparison, the value was significantly different (p<.05).The balance of autonomic nerve was changed, becoming close to the normal level in accord with applicable international standards(1.5)(p=.011). In multiple comparison, the value showed a significant difference(p<.05). Physical resistance to stress increased with the passage of time, but not statistically significant. Conclusions : This suggests that aromatherapy can be used as a nursing intervention which aims at alleviating symptoms related to the imbalance of autonomic nerve system such as headache, hot flashes, irregular heartbeat, nervousness, depression and anxiety.