발효조를 이용한 Monascus anka의 적색소와 황색소의 생산

강성국,임종환,정순택,김선재 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

Monascus anka를 이용하여 천연식용색소를 대량생산하기 위한 방법을 개발하기 위하여 발효조를 이용하여 적색소와 황색소의 색소생산의 최적 배양 조건과 생산된 색소의 색깔 특성을 조사하였다. Monascus anka 적색소와 황색소를 생산하였는데, 이들 색소는 균체내색소와 균체외 색소로 구분되었다. 균체의 적색소(ERP)는 494nm에서, 균체외 황색소(EYP)는 380nm에서, 균체내 적색소(IRP)는 506nm에서 그리고 균체내 황색소(IYP)는 388nm에서 최대 흡광도를 나타냈다. 적색소와 황색소 생산, 색조의 특성 및 균체증식력을 배양 온도, pH, 쌀가루 농도, peptone 농도, magnesium sulfate 농도, 통기량 및 교반속도에 대하여 조사한 결과 적색소 생산력은 30℃, 초기 pH 6.0, 쌀가루 농도 3~5%, peptone 농도 0.05%, magnesium sulfate 농도 0.25%, 통기량 0.1vvm 교반속도 300rpm의 조건에서 최대치를 보였으며, 이때 ERP, EYP, IRP 및 IYP는 각각 A_494nm 0.84, A_380 nm 0.71, A_506 nm 1.18, A_388 nm 1.10, L값은 각각 29.40, 6.44, 34,98, 8.94, a값은 각각 5.76, -1.08, 11.83, -1.55, b값은 각각 18-19, 3.41 27.26, 6.16 그리고 균체량은 7.4 g/l을 보였다. 또한 황색소 생산력은 온도의 경우 적색소 생산을 위한 최적온도 보다 높은 35℃ 부근에서 우수하였으며 초기 pH와 쌀가루 농도는 적색소 생산을 위한 최적조건과 같았고, 질소원과 무기염의 농도가 높을수록 그리고 통기량이 많을수록 황색소 생산력이 우수하였다. 적색소와 황색소 생산을 위한 발효조의 교반속도는 100~300 rpm이 적합하였다. Production of Red and Yellow Pigments from Monascus anda in a Jar Fermenter, Seong-Gook Kang, Jong-Whan Rhim, Soon-Teck Jung^* and Sun-Jae Kim. Department of Food Engineering. Mokpo National University Chonnam, 534-729, Korea - In order to develop the method for mass production of natural food colorant from Monascus anka, optimum cultivation conditions for producing red and yellow pigments by cultivating the mold in a jar fermenter and their color characteristics were investigated. The mold produced red and yellow pigments both intracellularly and extracellularly. These pigments showed unique light absorption characteristics with maximum absorption of 494, 380, 506, and 388 nm for extracellular red pigment (ERP), extracellular yellow pigment (EYP), intracellular red pigment (IRP), and intracellular yellow pigment (IYP), respectively. Optimum conditions for producing red pigments were found to be temperature 30℃, initial pH 6.0, rice powder 3~5%, peptone 0.05%, magnesium sulfate 0.25%, aeration rate 0.1vvm. Optimum temperature for producing yellow pigments was around 35℃ which is higher than that of producing red pigments. The initial pH rice powder concentration for producing yellow pigments were the same as those of producing red pigments. The higher concentration of nitrogen source and inorganic salt, aeration rate, the more the yellow pigments were produced. Them optimum agitation speed was 100~300 rpm for pigment production.

油壓 베인펌프의 內部 動的 壓力 特性에 關한 實驗的 硏究

鄭材鍊,李鍾純 全北大學校 1989 論文集 Vol.31 No.-

This paper presents an experimental study of the dynamic internal pressure within an oil hydraulic vane pump. The measurement of the dynamic internal pressures near the vane of a pressure balance type of an oil hydraulic vane pump with intravanes has been made to provide the essential information for the study of the pump dynamics and control, the pump design and the analysis of tribological problems in the sliding components. The influences of the discharge pressure and rotating speed of the vane on the dynamic pressure in four chambers surrounding a vane have been investigated. The results indicate that the surge pressures of the chambers at the instant of the start of discharge and closure are affected by the rotating speed, and the pressure in the intravane chamber maintains approximately constant values with remarkabel effect of pulsating discharge pressure. The profiles which have been presented in figures 4 through 7 indicate in detail the pressure distribution and acting forces of the vane to the camring.

례주(醴酒)의 제조와 품질특성

정순택,김선재 한국식생활문화학회 2001 韓國食生活文化學會誌 Vol.16 No.4

Li was a sweet beverage containing 2-3 percents ethyl alcohol made from malt by spontaneous fermentation from ancient custom to fifteenth century. Li was changed to the rice wine being a sweet beverage of low alcohol content using nuruk as starter and the sikhae which is non-alcoholic fermented beverage. Li was made for drinking and ceremony in Korea, China and Japan. It disappeared from the beverage items along with its method of manufacture from malt, but in Korean had made Li using nuruk until recent. We made Li according to Book of Imwon-Keongjae Ji (The book of country economy) methods for reappearance of Li. Fermentation characteristics for the production of Li with Saccharomyces cerevisiae, Saccharomyces bayanus and Saccharomyces sake were investigated. Among the yeast strains tested, Li fermented with S. sake showed higher alcohol production. Total sugar decreased considerably during the whole period of fermentation(30 hours), while ethyl alcohol content increased at 2.9∼83.52%. As the fermentation progressed, the pH decreased until the 30 hours of fermentation, while total acid increased during the same period. In fermentation of 36 hours, Li consisted of about 2.98∼3.52% of alcohol content, 5.3∼6.0% of total sugar, 1.45∼2.21㎎% of reducing sugar and total acidity were reached up to 24.4∼29.5㎎% for Li manufactured with S. cerevisiea sake, S. bayanus and S. sake.

LCC 영향도 다이어그램 開發에 관한 硏究

정순성,이정재 東亞大學校 建設技術硏究所 1999 硏究論文集 Vol.23 No.1

The purpose of this study is to suggest Life Cycle Costing Influence Diagram, which can be used on behalf of a Sensitivity Analysis and Simulation that have used with many problems. As the results of case study, new risk analysis method, Life Cycle Costing Influence Diagram, introduced here is consist with results of a Sensitivity Analysis and Simulation. The three features of Life Cycle Costing Influence Diagram as new risk analysis method can be summarized as follows: (1) While analyzing LCC, a decision-maker can make out overall situations easily as to each aspect of steps. (2) It is convenient to identify the most effective variables in LCC (The lower the slope is, the more sensitive the effect as to LCC is) (3) It enables to evaluate LCC manually as well.

660kW급 풍력발전기 로터의 강제 응답 해석에 관한 연구

정순철,김덕수,이재응 중앙대학교 기술과학연구소 2001 기술과학연구소 논문집 Vol.31 No.-

본 연구에서는 660㎾급 풍력발전 시스템용 유도발전기 로터의 강제 응답 해석을 수행하였다. 이를 위하여 유한요소법을 이용한 회전체 시스템의 모드 해석을 수행하였으며, 모드 실험 결과 나타난 고유진동수와 모드 형상의 오차를 줄이기 위하여 유한요소 모델의 구조 변경을 수행하였다. 또한 회전체 시스템의 안정성 해석을 위하여 로터에 작용하는 여러 가지 외력(불평형력, 전자기력등)에 대한 강제 응답 해석을 수행하였다. 그 결과 공진주파수는 운전주파수인 30㎐(1800rpm)의 약 4.5배의 크기에서 발생하였으며, 운전주파수에서의 최대 처짐은 풍력발전기 회전체 시스템의 자중에 의한 정적 처짐과 공극에 비하면 매우 미소하여 거의 무시할 만 하다. 따라서 로터에 작용하는 외력은 시스템의 안정성 큰 영향을 미치지 않는 것이라 결론 지을 수 있다. In this paper, the stability analysis of the rotor system in the wind-power generator was performed by using Finite Element Method. Experimental modal analysis of generator rotor system was performed and the result were used to update the finite element model. The stability analysis of wind-power generator rotor-bearing system was performed through harmonic analysis with all possible external forces including the electromagnetic force, the unbalance force, the torsional force, etc. As a result of stability analysis, the first natural frequency of the bearing-rotor system is 4.5 times as large as operating frequency. The dynamic deflection at operating frequency is quite smaller than static deflection which is also quite smaller than the air-gap. So it can be concluded that the external forces of the rotor have no effect on stability of the rotor-bearing system.

InSb의 결정성장 및 특성

정양준,양순철,이형재 木浦大學校基礎科學硏究所 1989 基礎科學硏究誌 Vol.7 No.-

InSb crystals, both doped and undoped, were grown using the zone-refining technique in vacuum. The unintentionally doped crystals exhibit n-type conduction with the lowest carrier concentration of ??. The pure crystals show intrinsic characteristics even at very low temperatures. Hence very heavily doped n-type crystals were grown in an effort to determine the electron behaviors, not disturbed by the other bands over a wide temperature range. All the InSb crystals prepared in this work were self-seeded along the (111) direction, and the volatilization turned out to be very efficient in reducing the residual impurities during the growing process. The electrical conductivity and Hall coefficient were measured as a function of temperature from 90 to 570K. Theoretical calculations were carried out using a three-band model with the ?? and two hole bands taking into account scattering mehanisms. The results were consistently fitted to the experimental data with the adjustable parameters used in describing the temperature variation of band gap, heavy hole effective mass??, deformation potential??. The final fitting was obtained with the following values, ??, ??, ??. At high temperatures polar optical scattering is dominant for electron scattering and electron-hole scattering becomes very important.

Bovine botulism outbreak associated with incidental consumption of presumably contaminated leftover food

( Jae Won Byun ),( Kyung Hyun Lee ),( Ha Young Kim ),( Soon Seek Yoon ),( Ji Youl Jung1 ),( Woo Seog Jeong1 ),( Jae Ku Oem ),( Jong Soo Lim ),( Myoung Heon Lee ),( You Chan Bae ) 한국예방수의학회(구 한국수의공중보건학회) 2013 예방수의학회지 Vol.37 No.1

Nine cattle among 18 in a native Korean herd died or were euthanized within 3 days. The affected cattle showed cowshed. The pigs and dogs had been fed leftover food originating from nearby restaurants, and the cattle could presumably reach the contaminated food in the pig troughs. Necropsy revealed a few chicken bones and red pepper pieces as well as large amounts of grain and rice straw hay mixed in the rumen. Botulism type D was isolated in the ruminal contents of one of the cattle. We speculated that the outbreak was associated with the cattle incidentally eating presumably contaminated leftover food from the pig trough.

나노크기의 은 촉매제조 및 특성분석

정유성,이상량,신재순,김영철,박남국 한국공업화학회 2002 응용화학 Vol.6 No.2

Nano-sized silver catalysts have been prepared by alcohol and dioctyl esterof sodium sulphosuccinic acid, anionic surfactant, Aerosol OT(AOT). TOD/TPR andethylene oxide activity have been studied to investigate the characteristics of silvercatalysts. The sizes of Ag particles were estimated to be 10∼16 nm and the peak ofsilver oxide was not observed by XRD except for metallic silver. The desorptionpeak of molecular oxygen by TPD/TPR uniqely observed at about 400 ℃ in thecatalyst prepared by water-alcohol method. However, in the catalyst prepared bymicroemulsion method, the desorption peaks of molecular and atomic oxygens wereobserved at about 400 ℃ and 600 ℃, respectivly. The catalyst prepared by water-alcohol method showed higher activity ethylene oxide than the catalyst prepared by microemulsion method. It eems due to the larger silver particles and easier desorption of molecular oxygen from the catalyst prepared by water-alcohol method.

β-Carotene의 酸化에 미치는 植物油의 影響

李淳宰,李萬正 韓國自然史博物學會 1981 生物과 自然 Vol.11 No.1

食品의 調理, 加工, 貯藏中에 일어나는 β-carotene의 酸化 破壞를 抑制하기 위한 實驗에서 結果는 다음과 같다. 즉 β-carotene에 添加한 植物性油가 빛에 의한 β-carotene의 酸化 破壞를 억제하는 效果가 있다. 그 酸化 抑制 結果가 가장 좋은 것은 옥수수기름이고 그 다음이 들깨기름, 콩기름, 참기름의 順位로 低下되었다. 添加한 기름의 濃度가 增加할수록 β-carotene의 酸化 抑制 效果가 增大하였다. For the protection from oxidation of β-carotene which occurred in the procedure of processing, cooking and storaging of food, some kinds of vegetable oils were added to the β-carotene solution, and then the rate of decolorization of β-carotene was detected after photo-oxidation under the illumination of 400 Lux with tungsten-filamented electroball at 11℃ for a given time in order to determine the β-carotene retention. As a results, oxidation of β-carotene was protected by vegetable oils which added to the β-carotene-acetone solution. In the protective effect, corn oil was most effective, and perilla oil, soybean oil and sesame oil were lowered orderly in its effects as an anti-oxidant, respectively. The protective dffect was increased by adding high concentration of oils to the solution of β-carotene. It seems likely, from studies of the protective activity of vegetable oils to the oxidation of β-carotene, that the protection was caused by the double bonds of unsaturated oils or contents of anti-oxidant; tocoperol.

가열유와 Vitamin E 투여가 흰쥐의 혈청 GOT, GPT 및 Alkaline Phosphatase 활성에 미치는 영향

이순재,박미향,정병두 대구효성가톨릭대학교 응용과학연구소 1991 응용과학연구논문집 Vol.1 No.-

가열유 투여에 따른 흰쥐 간장 조직의 과산화적 손상에 대한 연구와 식이중 V-E의 수준에 따라 가열유로 인한 간장조직의 과산화적 손상에 대한 항산화적 방어현상을 관찰하기 위하여 열화된 가열유(산가 4.02, carbonyl value 27.9, peroxide value 52.7)를 하루 총열량의 10%을 그리고 dl-α-tocopheryl acetate의 양을 달리하여 (0, 40, 200mg/kg of diet : OH group, 40H group, 200 H group) tube feeding하면서 3, 6주간씩 사육하고 실험종료후 혈청을 얻어 GOT, GPT, alkaline phosphatase 활성변화를 관찰하였다. 1. 가열유를 투여한 식이군은 대조군에 비해 혈청 GOT, GPT, AL-pase 활성도가 높았다. 2. V-E가 결핍된 OH식이군이 대조군에 비해 GOT, GPT, ALP 활성이 가장 높았고, 40H, 200H순위로 V-E첨가 수준이 높을수록 비례하여 이를 효소활성이 감소하였다. 3. 가열유의 투여기간이 길어질수록 효소 활성이 전반적으로 상승되었다. In order to investigate the liver damage due to heated oil intake and the antioxidative effect of vitamin E on it, rats were fed for 3 and 6 weeks by intubating heated corn oil with acid value of 4.02 at the level of 10Ca% and three different levels of vitamin E : 0, 40 and 200 mg/kg diet. Control group was fed fresh corn oil and 40 mg/kg diet of vitamin E. After each feeding period, serum glutamic oxaloacetatic transaminase(GOT), glutamic pyruvic transaminase(GPT), and alkaline phosphatase(AL-pase) activities were measured. GOT, GPT and AL-pase activities were higher in heated oil groups than control group. As dietary vitamin E level was increased, GOT, GPT and AL-pase activities were decreased progressively. These phenomena were more remarkable in 6 weeks of feeding than 3 weeks.