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( Bo Mi Kini ),( Chong Hae Hong ),( Tae Wook Hahn ) 한국예방수의학회(구 한국수의공중보건학회) 2012 예방수의학회지 Vol.36 No.2
Egg yolk immunoglobulin (IgY) is the antibody in egg yolk and can be produced in egg yolk by immunizing hens with antigens. IgY is functionally equivalent to mammalian lgG. It is found in the serum of the chicken and is passed from the mother chicken to the embryo via the egg yolk, a process that results in a high concentration of IgY in the egg yolk. The objective of this study was to evaluate the efficacy of specific IgY against enterotoxigenic Escherichia coil (ETEC) K99, Salmonella Typhimurium and Salmonella Choleraesuis that cause porcine bacterial diseases. To prepare specific IgY, Hy-Line Brown chickens were vaccinated with killed vaccine complex including E. coil 1(99, S. Typhimurium and S. Choleraesuis. The chicken egg yolk antibodies were purified from egg yolk by ammonium sulfate precipitation and the quality of the final preparation was confirmed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Titres of specific IgY in final preparations were measured by an enzyme-linked immunosorbent assay (ELISA). Antibody titers peaked at 3 weeks regardless the bacterial types and the similar patterns of immune response were observed for respective pathogens. In growth inhibitor test, specific IgY showed inhibitory effect on bacterial growth. After 0, 3, 6 and 12 hour of incubation with specific lgY (100 ag/nil, 250 /m1, 500 tig/inl), there was a significant decrease in the growth (A600nm) of E. coil K99, S. Typhimurium and S. Choleraesuis compared to nonspecific IgY and controls. In BALB/c mice, the effect of specific IgY (100 nig/kg, 250 mg/kg) on bacterial challenges was investigated by intramuscular injection and oral administration of bacteria. Mice treated with specific IgY showed high survival rate though there was no significant differences on blood biochemichal examinations between treated and untreated groups. These results indicate the potential of specific IgY for the treatment of porcine bacterial diseases caused by E. coil K99, S. Typhimurium and S. Choleraesuis.
세계의 구제역 전파,확산역학 모델 개발 현황과 Davis Animal Disease Simulation 모델 국내 적용 연구
윤하정 ( Ha Chung Yoon ),김한 ( Han Kim ),윤순식 ( Soon Seek Yoon ),김연주 ( Youn Ju Kim ),김병한 ( Byoung Han Kim ),( Jack Coleman ),( Tim Carpenter ) 한국예방수의학회(구 한국수의공중보건학회) 2011 예방수의학회지 Vol.35 No.4
Epidemic models on disease spread attempt to simulate disease transmission and associated control processes. This study reviewed published papers on epidemiological models for the management of foot-and-mouth disease in the world. In addition, an individual animal-based, spatially-explicit, stochastic disease transmission model, the Davis Animal Disease Simulation (DADS) model, was described in the frame of an international collaborative research project participating three countries: Republic of Korea, USA, and New Zealand. In this project, the Korean team is aiming at developing the most appropriate parameters for livestock and epidemiology of foot-and-mouth disease outbreaks. On the other hand, the purpose of foreign counterparts is validating their models: DADS (USA) and InterSpread Plus (New Zealand). Classification of farm types and preliminary estimations on the frequency of intra-herd contacts were also presented. This research project is expected to provide precious information to plan a strategy that will facilitate the eradication of foot-and-mouth disease from Korea.
Multiplex PCR을 이용한 Mycobacterium bovis와 Mycobacterium tuberculosis의 동정
장영부 ( Young Boo Jang ),김재명 ( Jae Myung Kim ),최재영 ( Jae Yeong Choe ),이햇님 ( Haet Nim Lee ),정석찬 ( Suk Chan Jung ),박영길 ( Young Kil Park ),구복경 ( Bok Kyung Ku ) 한국예방수의학회(구 한국수의공중보건학회) 2011 예방수의학회지 Vol.35 No.4
Tuberculosis (TB) is a significant disease for both humans and animals worldwide. The genus Mycobacterium includes several species that cause TB disease in humans and other animals. Amongst the members of the Mycobacterium tuberculosis complex (MTC), M. tuberculosis is mainly a human pathogen, whereas M. bovis has a broad host range and is the principal agent responsible for TB in domestic and wild mammals. M. bovis also infects humans, causing zoonotic TB through ingestion, inhalation. M. bovis accounts for only a small percentage of the reported cases of TB in humans. In recent years, TB in farmed deer has become a disease as public health importance in several countries. Nowadays, there has been rapid outbreak of bovine TB in cattle and deer in Korea. Investigations are needed to elucidate the relative importance of M. bovis on TB incidence in humans, especially in Korea where bovine TB remains a problem. Also, the human sources as the cause of animal infection, M. tuberculosis from the farm workers also important for TB control of animals in Korea. Differentiation between the causative organisms may only be achieved by sophisticated laboratory methods involving bacteriological characteristics, biochemical properties, and routine resistance to pyrazinamide (PZA). M. bovis shows inherently resistant to PZA whereas M. tuberculosis is susceptible to PZA. In this study, we developed a multiplex-PCR assay based on a 12.7-kb fragment for the differential detection of M. bovis and M. tuberculosis. A total of 131 M. tuberculosis complex isolates were randomly obtained from cattle and deers that were PPD positive. they all yielded M. bovis. M. tuberculosis was not isolated from animals. and, a total of 25 M. tuberculosis complex isolates which is resistant to PZA were obtained from patient. PZA resistant MTC in humans was caused entirely by M. tuberculosis. The multiplex-PCR protocol was highly species-specific and time saving for identification of M. bovis and M. tuberculosis. This multiplex-PCR assay will be easily used as a routine monitoring tool in veterinary and medical laboratories.
제주도 지역 종돈장에서의 돼지생식기호흡기증후군 혈청학적 유병률 조사
최은진 ( Eun Jin Choi ),이창희 ( Chang Hee Lee ),송재영 ( Jae Young Song ),임성인 ( Seong In Lim ),하종철 ( Jong Cheol Ha ) 한국예방수의학회(구 한국수의공중보건학회) 2011 예방수의학회지 Vol.35 No.1
This study was performed to estimate the seroprevalence of PRRSV in breeding farms in Jeju 2008 using a commercial enzyme-linked immunosorbent assay (ELISA) kit. The tested sera were randomly collected from a total of 1,947 sera from 9 breeding farms unvaccinated in Jeju. As a result, all breeding farms were seropositive for PRRSV. Seven hundred-eighty six of 1,947 sera (40.4%) were positive for PRRSV. Seropositve rate of PRRSV infection in 9 farms showed various levels: 1%, 8.9%, 9.1%, 43%, 46.9%, 48.2%, 51.6%, 60.9%, 85.5%, respectively. The results confirmed that PRRSV infection has been prevailing in breeding farms in Jeju. Also, these results must be taken into a consideration in strategy establishment for the control and eradication of PRRS.
국내 말전염성빈혈에 바이러스에 대한 혈청학적 조사 (2007~2009)
예정용 ( Jung Yong Yeh ),이지혜 ( Ji Hye Lee ),박지용 ( Jee Yong Park ),조인수 ( In Soo Cho ),최윤기 ( Yun Gi Choi ),양선주 ( Sun Joo Yang ),경순구 ( Soon Goo Kyung ),안계명 ( Kei Myung Ahn ),김희파 ( Hee Pah Kim ),문진산 ( Jin Sa 한국예방수의학회(구 한국수의공중보건학회) 2011 예방수의학회지 Vol.35 No.3
Horse population has been gradually increasing with the growth of the racing industry in Republic of Korea (ROK). Approximately 22,941 horses including native ponies were raised on 1,142 farms in 2006. Great care has been taken to prevent viral, bacterial and parasitic infections in horses. However, there has been few reports on equine infectious anemia (EIA) in ROK until recently since 1987. Therefore, we conducted a serological survey of EIA in ROK from 2007 through 2009. Using the agar gel immunodiffusion test, a total of 2,601 horses were tested in Foreign Animal Disease Division of Animal, Plant, and Fisheries Quarantine and Inspection Agency. This survey found no serological evidence of EIA presence in ROK. Although our surveillance found no evidence of EIA in ROK during the surveillance period, it is possible that EIA could be introduced into ROK in the near future. Increased cooperation between the government and other agencies, such as horse-racing authorities, is important for both the early detection of the disease and the development of effective veterinary and public health strategies.
( Suk Jae Park ),( Tae Jun Kwon ),( Jae Ho Bae ),( Min Soo Kang ),( Huun Seok Chae ),( Jae Hoon Woo ),( Seol Hwa Park ),( Hyoung Seok Yang ),( Wan Shol Kang ),( Jin A Ko ),( Nam Young Kim ) 한국예방수의학회(구 한국수의공중보건학회) 2015 예방수의학회지 Vol.39 No.4
Viral respiratory infections are common in horses, notably equine herpesvirus infection and equine influenza, which primarily initiate secondary bacterial respiratory infections such as strangles caused by Streptococcus equi equi. A decline in the production of stallions has been associated with these respiratory diseases leading to adverse financial implications. This study investigated the antibody responses against respiratory diseases in horses from Jeju Island a year after vaccination. A low level of equine herpesvirus type 1 (EHV-1) (11.36%) antibodies was detected from stallions, however a high level of EHV-4 (95.84%) antibodies was detected from horses without vaccination against this infection suggesting that EHV-4 is ubiquitous in this horse population. In case of equine influenza, ranch stallions showed low positive rate (12.06%) whereas stallions from Subtropical Livestock Research Institute displayed higher positive rate (81.32%). Antibody responses against equine influenza and strangles revealed positive rates of 26.32% and 55.12%, respectively. These findings may draw attention towards the importance of developing an improved disease prevention and/or immunization program that will effectively control respiratory diseases in horses.
Current prevalence of Batrachochytrium dendrobatidis in imported and native frogs in South Korea
Suk Kim,Lauren Togonon Arayan,Tran Xuan Ngoc Huy,Alisha Wehdnesday Bernardo Reyes,Huynh Tan Hop,Byoung Youl Cho,Jae Bum Cho,Jin Ha Yu,Son Il Pak 한국예방수의학회(구 한국수의공중보건학회) 2017 예방수의학회지 Vol.42 No.1
In Korea, a serious amphibian disease caused by the fungus Batrachochytrium dendrobatidis (Bd) has been reported from historical samples collected in the 1900s. In this study, we continue to evaluate the current prevalence of chytridiomycosis in the Korean Peninsula and we include imported frogs from America to our analysis. Non-invasive skin swabs were taken from 275 apparently healthy frogs, and Bd was detected in five free living frogs by the nested PCR protocol consisting of two species: Bombina orientalis and Rana catesbeiana, from Gyeongnam and Cheonbuk provinces. These frogs comprised about 2% of the total number of free living samples. This study might be useful for understanding amphibian chytridiomycosis in Korea.
Antioxidant effects of Phloroglucinol on gamma radiation-induced oxidative stress on mice skin
Areum Kim,KHINM Herath,Youngheun Jee,Taeseong Moon,Jinhee Cho,Sojin Bing,Ginnae Ahn 한국예방수의학회(구 한국수의공중보건학회) 2017 예방수의학회지 Vol.42 No.1
The seaweed Ecklonia cava, a brown algae abundant in JeJu Island, South Korea, has large amounts of the polyphenol compound phloroglucinol (PG, 1,3,5-trihydroxybenzene), which has been proposed to exert interesting biological properties including antioxidant and radioprotective effects against ionizing radiation-induced damages in various cells and tissues. To identify antioxidant and radioprotective effects of PG in skin tissues, we exposed mice to 8.5 Gy whole body irradiation (WBI) at day 6 after depilation with and without PG treatment. In PG treated cases, PG was applied twice, once at 17.8 hours before and then at the time of WBI. At 8 hours after WBI, a reduction in the formation of thiobarbituric acid-reactive substrates (TBARS) was observed in the PG treated group. Upon western blot analysis, PG treatment overexpressed the MnSOD, catalase, and GPx-1, although the difference was not significant. In parallel with the results of western blot analysis, the percentage of MnSOD-and catalase-positive cells was significantly increased at 8 and 24 hours after WBI, while no significant difference was observed over 48 hours in PG treated skins. Moreover, PG treatment increased the percentage of Ki-67 positive cells compared with that of irradiated only mice at 8 hours after WBI. Our results suggest that PG is effective at attenuating oxidative stress, and that the promotion of antioxidant enzymes such as MnSOD and catalase may be an important aspect for its radioprotection in skin.