Pretreatment with Lycopene Attenuates Oxidative Stress-Induced Apoptosis in Human Mesenchymal Stem Cells

( Ji Yong Kim ),( Jai Sung Lee ),( Yong Seok Han ),( Jun Hee Lee ),( Inhyu Bae ),( Yeo Min Yoon ),( Sang Mo Kwon ),( Sang Hun Lee ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Human mesenchymal stem cells (MSCs) have been used in cell-based therapy to promote revascularization after peripheral or myocardial ischemia. High levels of reactive oxygen species (ROS) are involved in the senescence and apoptosis of MSCs, causing defective neovascularization. Here, we examined the effect of the natural antioxidant lycopene on oxidative stress-induced apoptosis in MSCs. Although H2O2 (200 mM) increased intracellular ROS levels in human MSCs, lycopene (10 μmM) pretreatment suppressed H2O2-induced ROS generation and increased survival. H2O2-induced ROS increased the levels of phosphorylated p38 mitogen activated protein kinase (MAPK), Jun-N-terminal kinase (JNK), ataxia telangiectasia mutated (ATM), and p53, which were inhibited by lycopene pretreatment. Furthermore, lycopene pretreatment decreased the expression of cleaved poly (ADP ribose) polymerase-1 (PARP-1) and caspase-3 and increased the expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), which were induced by H2O2 treatment. Moreover, lycopene significantly increased manganese superoxide dismutase (MnSOD) expression and decreased cellular ROS levels via the PI3K-Akt pathway. Our findings show that lycopene pretreatment prevents ischemic injury by suppressing apoptosis-associated signal pathway and enhancing anti-oxidant protein, suggesting that lycopene could be developed as a beneficial broad-spectrum agent for the successful MSC transplantation in ischemic diseases.

항정신병약물 사용 중인 정신분열병 환자에서 올란자판으로의 교체 방법에 관한 연구(II) : Comparison of Safety 안전성 비교

안용민,권용실,권준수,민성호,박두병,양문정,소형석,송종호,신윤식,우행원,유범희,이홍석,정한용,한창환,김용식 大韓神經精神醫學會 2002 신경정신의학 Vol.41 No.5

연구목적: 이 다기관 공동임상연구는 사용 중인 항정신병약물을 ’직접 교체 방법’또는 ’시작-감량 교체 방법’중 한 가지 방법으로 올란자핀으로 교체한 후, 안정성 측면에서 두 교체 방법 간의 비교와 교체후의 변화를 관찰하기 위한 것이다. 방법: 국내 13개 병원의 입원 및 외래에 내원한 환자들 중 ICD-10 지단기준으로 정신분열병에 해당되며, 임상적으로 항정신병약물 교체가 필요한 환자를 대상으로 하였다. 두 가지 교체 방법 중 한 가지를 무작위로 피험자에 적용하였으며, ’직접 교체 방법’에 배정된 경우에는 사용중인 항정신병약물을 일시에 중단하고 10㎎의 올란자핀을 바로 투여하였고, ’시작-감량 교체 방법’에 배정된 경우는 10㎎의 올란자핀 투여하고 2주에 걸쳐서 기존 약물을 감량하여 중단하였다. 올란자핀 사용기간은 총 6주이며, 용량은 5∼20㎎ 범위로 제한하였다. 한정성 평가를 위해서 체중, 생명징후, 자발적인 이상반응 복, 실험실 검사 그리고 Simpson-Angus Scale(SAS), Barnes akathisia rating scale(BARS), Abnormal involuntary movement scale(AIMS). Liverpool University neuroleptic side effect rating scale(LUNSERS)등을 이용하였다. 결과: 총 103명의 정신분열병 환자를 대상으로 하였다. 사용한 올란자핀의 용량, 벤조디아제핀의 병용률, 탈락률과 탈락 사유, 자발적인 이상반응 보고, 생명징후, 실험실 검사 그리고 대부분의 부작용 척도 상에서 임상적으로 의미 있는 차이를 두 교체 방법간에 발견하지 못하였다. 다만 AIMS의 감소는 ’직접 교체 방법’군에서 보다 적었고, 항콜린제의 병용률은 ’시작-감량 교체 방법’군에서 보다 많았다. 기저 상태에서 전체 피험자의 SAS와 BARS 점수는 각각 3.5점과 1.8점이었으며 70% 이상의 피험자가 고프로락틴 혈증을 보였다. 올란자핀으로 교체한 후, SAS, BARS, AIMS 점수의 유의한 감소가 있었으며 고프로락틴 혈증을 보인 피험자 분율도 약 30%이하로 감소하였다. 그러나 교체 방법과 상관없이 올란자핀 교체 후 유의한 체중 증가가 있었다. 결론: 이 연구를 통해 교체 방법에 관계없이 비교적 안전하고 용이하게 올란자핀으로 교체 할 수 있음을 알 수 있었다. 그리고 기존 항정신병약물을 올란자핀으로 교체함으로써 일부 부작용들을 줄일 수 있음을 간접적으로 관찰할 수 있었다. 하지만 이 연구는 여러 제한점과 문제점을 지니고 있기 때문에 보다 체계적인 연구를 통해 검정이 필요하리라 생각된다. Objectives: This multicenter clinical trial involving 13 hospital sites compared the safely of switching to olanzapine between ’direct switching method’ and ’start-tapering switching method’. Method: This study included both inpatients and outpatients who fulfilled the criteria for schizophrenia as defined in the ICD-10, and were in need to be appropriate for switching antipsychotics. Subjects were randomly assigned to one of the two switching methods. For ’direct switching method’group, previous antipsychotics were abruptly discontinued and 10㎎ of olanzapine was administered, and previous antipsychotics was gradually tapered for 2 weeks. Olanzapine was used for 6 weeks and the dose was adjusted within the range of 5-20㎎. The safety of switching to olanzapine was measured with vital sings including body weight, adverse events reported spontaneously, laboratory tests, and various scales such as Simpson-Angus Scale(SAS), Barnes Akathisia Rating Scale(BARS). Abnormal Involuntary Movement Scale(AIMS), and Liverpool University Neuroleptic Side Effect Rating Scale(LUNSERS). Results: 103 patients were switched to olanzapine in this study. The comparison between two switching methods did not show any significant difference in the dosage of olanzapine used, the concomitant use of benzodiazepine, the rate and reasons of drop-out, the adverse events, vital signs, laboratory tests, and most scales for measuring side-effects. However, the decrease in AIMS scores was significantly lower in ’direct switching method’ group, and the concomitant use of anticholinergics was comparatively greater in ’start-tapering switching method’ group. At baseline, SAS and BARS scores were 3.5 and 1.8 points respectively, and more than 70% of the subjects showed hyperprolactinemia. After switching to olanzapine, SAS, BARS, and AIMS scores were significantly decreased and the proportion of the patients with hyperprolactinemia was also decreased to less than 30%. However significant weight gain after the treatment of olanzapine was observed regardless of switching method. Conclusion: This study may suggest that switching to olanzapine can be done with relatively high safety regardless of switching methods and olanzapine can significantly decrease some side-effects induced by other antipsychotics.

Pretreatment with Lycopene Attenuates Oxidative Stress-Induced Apoptosis in Human Mesenchymal Stem Cells

Kim, Ji Yong,Lee, Jai-Sung,Han, Yong-Seok,Lee, Jun Hee,Bae, Inhyu,Yoon, Yeo Min,Kwon, Sang Mo,Lee, Sang Hun The Korean Society of Applied Pharmacology 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.6

Human mesenchymal stem cells (MSCs) have been used in cell-based therapy to promote revascularization after peripheral or myocardial ischemia. High levels of reactive oxygen species (ROS) are involved in the senescence and apoptosis of MSCs, causing defective neovascularization. Here, we examined the effect of the natural antioxidant lycopene on oxidative stress-induced apoptosis in MSCs. Although $H_2O_2$ ($200{\mu}M$) increased intracellular ROS levels in human MSCs, lycopene ($10{\mu}M$) pretreatment suppressed $H_2O_2$-induced ROS generation and increased survival. $H_2O_2$-induced ROS increased the levels of phosphorylated p38 mitogen activated protein kinase (MAPK), Jun-N-terminal kinase (JNK), ataxia telangiectasia mutated (ATM), and p53, which were inhibited by lycopene pretreatment. Furthermore, lycopene pretreatment decreased the expression of cleaved poly (ADP ribose) polymerase-1 (PARP-1) and caspase-3 and increased the expression of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), which were induced by $H_2O_2$ treatment. Moreover, lycopene significantly increased manganese superoxide dismutase (MnSOD) expression and decreased cellular ROS levels via the PI3K-Akt pathway. Our findings show that lycopene pretreatment prevents ischemic injury by suppressing apoptosis-associated signal pathway and enhancing anti-oxidant protein, suggesting that lycopene could be developed as a beneficial broad-spectrum agent for the successful MSC transplantation in ischemic diseases.

니트섬유강화 복합재료의 기계적 성질에 관한 연구

한길영,이동기,최병기,오환교,신용욱,전수영 조선대학교 에너지.자원신기술연구소 1999 에너지·자원신기술연구소 논문지 Vol.21 No.2

Using conventional textile techniques such as weaving, braiding, knitting and stitching it is possible to produce a wide range two and three dimensional fiber preforms. However, so far only a limited attention has been given to knitted fabrics in composite industry. This is mainly due to the opinion that knitted fabric reinforced composites posses low mechanical properties owing to their looped fiber architecture. But it is possible to obtain desired mechanical properties by selecting proper knitted fabric structure. In this paper, tensile failure load and displacement of one yarn, four yarns and knitted fabrics of ten yarns of kevlar fiber were determined experimentally in the unidirection. Mechanical properties of plain weft knitted fabrics reinforced composite intrusion beam for car side door application were investigated experimentally, which compared with desire value of American Fedral Motor vehicles Safety Standard (FMVSS) and presented the results.

당뇨병환자에서 뇨증 Fibronectin농도의 증가에 관한 연구

한승범,조준승,손건영,서성문,박근용,조성래,박규영,박정모,이인규,여준기 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4

Plasma fibronectin is an α₂-glyoprotein, which is produced by vascular endothelial cells. Raised level of plasma fibronectin has been observed in diabetic patients particularly in the presence of microvascular complications. However, no available data exist about urine level of this glycoprotein in diabetic patients. We measured urine fibronectin level by ELISA methods in 54 diabetic patients who have microvascular comlications or not. The following results were obtained. 1) Urine fibronectin level(㎍/g creatinine) in diabetic group(1740.0±678.0) is increased compaired with those in normal control group(471.0±59.0). but this results were not significantly different among two groups(P>0.05). 2) Urine fibronectin level(㎍/g creatinine) was significantly increased in patients with nephropathy (6188.0±3144.0) compared with those in normal control group(471.0±59.0) and patients without nephropathy(645.0±251.0)(P<0.001). 3) There were a significant correlation between BUN, creatinine, creatinine clearance, 24hr urine total protein and urine fibronectin level in diabetic patients. Our data suggest that urine fibronectin excretion level might be used as a sensitive guide for diabectic nephropathy.

냉각탑용 수분배기의 특성에 관한 연구

전유신,최석천,이용훈,김세현,정효민,정한식 경상대학교 해양산업연구소 2002 해양산업연구소보 Vol.15 No.-

The main purpose of this research is the investigation of water distributer. The water distributer of this research is installed in evaporative cooling tower system. The performance of this cooling tower system depend on the evaporative surface, and there are many kinds of methods for increasing the evaporative surface. In this research, we introduced the two kinds of spraying system, one is the hole type with hole diameter 1, 2 and 3mm, and the other is the spray nozzle types with nozzle tip diameter of 1 and 2mm. As the experimental results, the case of diameter 2mm showed the best performance.

산소 소모법에 의한 액체 연료의 열발생율 평가

한용식,김명배,최준석 韓國火災ㆍ消防學會 1998 한국화재소방학회논문지 Vol.12 No.3

액체연료들의 열발생율이 산소 소모법에 의해 측정되었다. 산소 소모법은 화재 시에 경험되어지는 대부분의 연료에 대해서 소모된 산소단위 질량당 발생된 열량이 근사적으로 같다는 원리에 기초하고 있다. 산소농도의 측정에는 상자성 방식의 가스 분석기가 사용되었고, CO_2 및 CO가스 농도 측정은 적외선 방식의 분석기에 의해 이루어졌다. 가스 분석기들의 시간지연은 고려하지 않았다. 배기가스 측정방법에 따른 결과들을 비교 검토하였다. The heat release rate of liquid fuels is estimated by oxygen consumption technique. This method is based on the generalization that the heat release rate of combustion per unit of oxygen consumed are approximately the same for most fuels commonly encountered in fires. The oxygen concentration is measured by analyzer of paramagnetic type. The concentrations of CO_2 and CO gas are measured by analyzer of Infra-Red type. Time delays of analyzers are ignored. Results acqired from measuring techniques of exhaust gas concentrations are compared with each other.

DAF에서 압력에 따른 미세기포의 크기특성

한무영,박용효,이준,심재수 대한상하수도학회 2002 상하수도학회지 Vol.16 No.2

Although DAF (Dissolved Air Rotation) has been successfully accepted for water and wastewater treatment, the fundamental characteristics of the process have not been fully investigated. According to recent theoretical work on DAF, bubble size is one of the most important factors that affect the DAF efficiency. It is well known that the size of bubbles in DAF is mostly affected by applied pressure. In this study, a newly developed Particle Counter Method (PCM) is introduced and compared with image analysis and verified. Using this PCM, the size of bubbles in DAF is measured under various pressure conditions from 2-6atm. Through a set of experiments, it was found that bubble size decreases as the pressure increases up to 3.5atm. This is a critical pressure after which bubble size does not decrease. According to the experimental results, it is not only costly but also unnecessary to maintain pressure more than 3.5atm if the goal is only to generate smaller bubbles for better removal efficiency in DAF operational practice.

스위치드 릴럭턴스 전동기를 위한 구동시스템의 역률개선과 고효율에 관한 연구

한대희,최준혁,윤용호,정동효,김도군,원충연 성균관대학교 2003 학술회의지원논문목록집 Vol.2003 No.-

Switched Reluctance Motor (SRM) offers the advantages of simple and robust motor construction, high speeds and high efficiencies over a wide operating range of torque and speed, excellent controllability. However, SRM has the disadvantages of high current harmonics, and low power factor because of a capacitor filter is inserted in the power converter and inductance of SRM is high, it has pulse waveform of current. This paper deals with an energy efficient converter fed SRM system with the reduced harmonics and improved power factor and with higher efficiency. The validity of the proposed scheme is verified via experiment. We are implemented the proposed control system using 8OC196KC micro-controller.

면진구조의 동적특성에 따른 구조물의 진동거동에 관한 연구

김용주,강병두,전대한,김재웅 동아대학교 건설기술연구소 2001 硏究報告 Vol.25 No.1

Base isolation systems consists essentially of the installation of mechanisms which decouple the structure, and/or its contents, from potentially damaging earthquake-induced ground, or supports, motions. This decoupling is achieved by increasing the flexibility of the system, together with providing appropriate damping. In this paper, the effectiveness of base isolated structures under a variety conditions are evaluated and compared. The results of this study are as follow; Increasing the natural period and the damping capacity of the base isolated structures decreases structure's response to earthquake load. Base isolation can enhance the seismic performance of structures by increasing the fundamental period of the structure to beyond the energy-containing periods of earthquake motion, and by providing additional damping to dissipate motions that do develop. If period of base structure is longer than that of main structure, the natural period of combined structure approaches to that of base structure.