http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
정동효,유진영 한국농화학회 1989 Applied Biological Chemistry (Appl Biol Chem) Vol.32 No.3
A bacterium synthesizing extracellular polysaccharide was isolated from soil and identified as Enterobacter agglomerans. The polysaccharide was found to be glucan polymer containing glucose and galactose in a molar ratio of 1 : 1.1. The aqueous solution was very viscous. The viscosity of 1% solution was 264 mPa.s. at 42 sec^(-1) and yield stress was 4.89 Pa. The polysaccharide solution did not have thermal stability but pH and salt stability.
정동효 한국농화학회 1969 Applied Biological Chemistry (Appl Biol Chem) Vol.12 No.1
1. Crude cellulase extracted from wheat bran media of Chaetomium globosum with pH 7.0 Mcllvaine buffer was fractionated by precipitation with ammonium sulfate and by treatment with the cellulose powder, DEAE-Sephadex A-25 and Amberite XE-65 (IRC-50) column chromatography. 2. Consquently two cellulases C-1 and C-2 were obtained by cellulose column chromatography. Cellulose C-1 was a powerful CMC-saccharifying and CMC-liquefying activity but cellulose C-2 was stronger CMC-liquefying activity compared to CMC-saccharifying activity and cellulase C-2 had smaller protein than that of cellulose C-1. And cellulose C-2 was fractionated by DEAE-Sephadex A-25 column chromatography into cellulase C-1-1 and cellulose C-1-2. 3. It can be obtained, therefore, that cellulose produced Chaelomium globosum consisted, at least, of three cellulases C-2, C-1-1 and C-1-2. 4. Cellulose C-1-1 was homogenous in the ultraviolet and the ultracentrifuge pattern. And cellulose C-1-1 had enzyme for CMC-saccharifying activity. 5. The optimum pH for the enzyme activity of cellulose C-1-1 was 4.0 in any methods of meas urement reducing sugar and viscosity. The optimum temperature was 40℃ in any methods. 6. The pH stability of cellulase C-1-1 was within pH 5.0 to pH 6.0 at 40℃ and fairly stable in acidic solution. 7. The heat stability was below 50℃ at pH 4.0 and complete heat inactivation of this cellulase occurred at 70℃.
물리화학적 및 관능적 특성에 의한 국내외산 녹차의 품질평가
정동효,손태화,신동화,이상효,오상룡 한국농화학회 1988 Applied Biological Chemistry (Appl Biol Chem) Vol.31 No.3
To establish the evaluation method of Korean green tea, the correlation between physicochemical characteristics and sensory scores by the expert panels were investigated icing 15 hinds of green tea manufactured in 1985 from Korea, Taiwan and Japan. Tannin, caffeine and total free amino acid contents of Korean green tea are higher than foreign ones, but the chlorophyll content is the highest in Japanese high grade green tea. Sixteen free amino acids including theanine were identified, and theanine, glutamic acid, aspartic acid and arginine contents which affect the brothy taste of green tea infusion were highest in Korean green tea. Sensory score for the color of Korean green tea infusion were lower than Japanese tea, but the flavor and taste were she best. In multiple regression analysis, the quality affecting factors of the green tea were elucidated as the caffeine(X₁), chlorophyll b(X₂), total free amino acid contents(X₃), and L value(X₄) of green tea infusion. The regression equation was expressed as Y=-21.5820 + 2.1691X + 0.0130X₂+ 0.2314X₃- 0.0001X₄ and then R² was 0.8919.
효모 유전자 발현용 (發現用) Promoter 개발에 관한 연구
정동효,정호권,박준희,심상국 ( Dong Hyo Chung,Ho Kwon Chung,Joon Hee Park,Sang Kook Shim ) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.1
The purpose of this study was the development of promoter for the lacZ` gene. Two heterologous promoter Ⅰ and Ⅱ of lacZ` gene were isolated from chromosomal DNA Bam HI fragment of yeast. The size of the promoter Ⅰ was estimated to be 2.5 kb and β-galacrosidase activity was 124.6 U/㎎ protein, and the size of the promoter Ⅱ was 4.0 kb and its β-galactosidase activity was 168.8 U/㎎ protein, respectively. The stability of the recombinant YEp plasmid in the transformant was from 52.7 to 67.4% at minimal medium. YIp plasmid was constructed from YEp plasmid, and expressed both in E. coli and yeast. The promoter Ⅰ aid Ⅱ iso-laced from yeast chromosomal DNA can be used for promoter of plasmid YEp and YIp.
정동효 한국식품산업협회 1973 좋은식품 Vol.16 No.-
21세기 초엽에 세계의 인구는 현재의 34억2천만의 약 2배인 66억에 달한다고. UNESCO는 경종을 울리고 있다. 식량중 특히 동물성 단백질의 부족은 약 6,000만톤으로 추산된다. 이 식량부족을 해결시키는 방법으로는 (1) 재래의 농수축산물의 증산 (2) 식량의 세계적 유통의 개선 (3) 새로운 자원의 개발 등으로 생각된다.
Heterothallic Saccharomycopsis lipolytica 의 유성생활환(有性生活環)의 유전적 해석
정동효,조석금 한국농화학회 1986 Applied Biological Chemistry (Appl Biol Chem) Vol.29 No.1
A yeast strains, CJ 2, CJ 7 and CJ 8, isolated from soil and contaminated cheese, mated with authentic strains of Saccharomycopsis lipolytica and were identified as Saccharomycopsis lipolytica with mating A, B and B, respectively. The strain CJ produced large amount of isocitric acid in glucose and n-hexadecane medium as compared with another strains. All strains produced larger amount of citric acid in n-hexadecane medium as compared with glucose medium, and citric acid production of diploids was greater than that of the parental haploid strains. The specific activity of isocitrate lyase in n-hexadecane grown cells was 15∼20 times greater than that in glucose-grown cells, but the specific activity of citrate synthetase was not so influenced by carbon source. Little correlation between citric acid production and the specific activity of these enzymes was noticed irrespective of strains and ploidy.