Homing and Restorative Effects of Bone Marrow-Derived Mesenchymal Stem Cells on Cisplatin Injured Ovaries in Rats

Liu, Jiabin,Zhang, Haiying,Zhang, Yun,Li, Nan,Wen, Yuku,Cao, Fanglei,Ai, Hao,Xue, Xiaoou Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.12

Premature ovarian failure (POF) is a long-term adverse effect of chemotherapy treatment. However, current available treatment regimens are not optimal. Emerging evidence suggests that bone marrow-derived mesenchymal stem cells (BMSCs) could restore the structure and function of injured tissues, but the homing and restorative effects of BMSCs on chemotherapy injured ovaries are still not clear. In this study, we found that granulosa cell (GC) apoptosis induced by cisplatin was reduced when BMSCs were migrated to granulosa cells (GCs) in vitro. Chemotherapy-induced POF was induced by intraperitoneal injection of cisplatin in rats. BMSCs labeled with enhanced green fluorescent protein (EGFP) were injected into the rats via the tail vein to investigate the homing and distribution of BMSCs in vivo. The number of BMSCs in the ovarian hilum and medulla was greater than in the cortex, but no BMSCs were found in the follicles and corpus lutea. In addition, the BMSCs treatment group's antral follicle count and estradiol levels increased after 30 days, compared with the POF group. Hence, our study demonstrates that intravenously delivered BMSCs can home to the ovaries, and restore its structure and function in POF model rats.

Enhancing the Ag Precipitation by Surface Mechanical Attrition Treatment on Cu-Ag Alloys

Jiabin Liu,Lehao Zhang,Jingjing Liu,Liuyi Huang,Hao Gu,Youtong Fang,Liang Meng,Jian Zhang 대한금속·재료학회 2016 METALS AND MATERIALS International Vol.22 No.5

The influence of surface mechanical attrition treatment (SMAT) on Ag precipitation in Cu-Ag alloys was investigated. Cu-6 wt% Ag was melt, cold rolled and solution treated to be Cu-Ag solid solution, which was either aged at 250 and 350 °C for 24 h directly or SMAT-ed before aging. Ag precipitates were hard be found in the directly aged Cu-Ag sample while they were observed clearly in the SMAT-ed counterpart at 250 °C. The Ag precipitates formed in the surface layer by SMAT are much coarser than those in the un-SMAT-ed sample. It is obvious that the precipitating behavior of Ag was promoted significantly by SMAT approach. A large number of defects were generated by SMAT and they were acting as fast atomic diffusion channels that facilitated the atomic diffusion of Ag.

Homing and Restorative Effects of Bone Marrow-Derived Mesenchymal Stem Cells on Cisplatin Injured Ovaries in Rats

Jiabin Liu,Haiying Zhang,Yun Zhang,Nan Li,Yuku Wen,Fanglei Cao,Hao Ai,Xiaoou Xue 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.12

Premature ovarian failure (POF) is a long-term adverse effect of chemotherapy treatment. However, current available treatment regimens are not optimal. Emerging evidence suggests that bone marrow-derived mesenchymal stem cells (BMSCs) could restore the structure and function of injured tissues, but the homing and restorative effects of BMSCs on chemotherapy injured ovaries are still not clear. In this study, we found that granulosa cell (GC) apoptosis induced by cisplatin was reduced when BMSCs were migrated to granulosa cells (GCs) in vitro. Chemo-therapy-induced POF was induced by intraperitoneal injection of cisplatin in rats. BMSCs labeled with enhanced green fluorescent protein (EGFP) were injected into the rats via the tail vein to investigate the homing and distribution of BMSCs in vivo. The number of BMSCs in the ovarian hilum and medulla was greater than in the cortex, but no BMSCs were found in the follicles and corpus lutea. In addition, the BMSCs treatment group’s antral follicle count and estradiol levels increased after 30 days, compared with the POF group. Hence, our study demonstrates that intravenously delivered BMSCs can home to the ovaries, and restore its structure and function in POF model rats.

Identification and Characterization of NDM-1-producing Hypervirulent (Hypermucoviscous) Klebsiella pneumoniae in China

Zhou Liu,Yi Gu,Xin Li,Yanyan Liu,Ying Ye,Shihe Guan,Jiabin Li 대한진단검사의학회 2019 Annals of Laboratory Medicine Vol.39 No.2

Background: Carbapenem-resistant hypervirulent (hypermucoviscous) Klebsiella pneumoniae (CR-HMKP) poses a significant public health challenge. We investigated its epidemiology and molecular characteristics in a tertiary care hospital in eastern China. Methods: CR-HMKP were identified among 106 non-duplicated carbapenem-resistant K. pneumoniae isolates (from June 2013 to September 2017) using the string test. The pulsotype (PT) and sequence type (ST) of CR-HMKP isolates were determined using pulsed-field gel electrophoresis and multilocus sequence typing. Resistance determinants, capsular serotypes, and virulence genes were detected by PCR and sequencing. Representative isolates from each PT were selected, and their virulence phenotypes were established using the serum killing and Galleria mellonella lethality assays. Results: Of the 106 isolates, 13 (12.3%) were CR-HMKP. Seven were positive for blaNDM-1 and shared the same genotype (PT5/ST1764); the others were positive for blaKPC-2, belonged to ST11, and were divided into four different PTs. The serotype of all blaNDM-1-positive isolates was K64, while that of blaKPC-2-positive isolates were K47 (N=4) and K64 (N=2). The NDM-1-producing HMKP isolates were positive for aerobactin, exhibited high serum resistance, and elicited significantly increased larval mortality compared with the other isolates. All patients had received invasive treatment prior to infection by NDM-1-producing HMKP. The infections occurred between July and August 2016 and were hospital-acquired. Conclusions: NDM-1-producing HMKP ST1764 isolates were identified; this is the first report worldwide on an outbreak of nosocomial infection caused by these isolates. Effective surveillance and strict infection control strategies should be implemented to prevent CR-HMKP dissemination.

Autogenous Shrinkage and Crack Resistance of Carbon Nanotubes Reinforced Cement‑Based Materials

Yanming Liu,Tao Shi,Yujing Zhao,Yuan Gu,Zhifang Zhao,Jiabin Chen,Bingmiao Zheng,Shichong Shi 한국콘크리트학회 2020 International Journal of Concrete Structures and M Vol.14 No.5

Cracking caused by shrinkage deformation of cement-based materials at early age is a major problem leading to material failure in restrained conditions. Carbon nanotubes (CNTs) are incorporated into cement-based materials, and the autogenous shrinkage and crack resistance of the new composite materials obtained by linear shrinkage and ring tests are studied to solve the destruction of the materials caused by the shrinkage of cement-based materials. The results showed that addition of CNTs significantly inhibited the autogenous shrinkage of cement-based materials with maximum reduction rate above 40%. CNTs also significantly improved the cracking resistance of cement-based materials. The optimal effect was noticed at CNTs content of 0.1 wt%. The incorporation of CNTs not only inhibits the autogenous shrinkage of cement-based materials, but also inhibits the drying shrinkage of cement-based materials to some extent. Therefore, carbon nanotubes have the potential to solve the destruction of materials caused by shrinkage of cement-based materials.

Neural Network Based Adaptive Fuzzy PID-type Sliding Mode Attitude Control for a Reentry Vehicle

Zhen Jin,Jiabin Chen,Yongzhi Sheng,Xiangdong Liu 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.1

This work investigates the attitude control of reentry vehicle under modeling inaccuracies and externaldisturbances. A robust adaptive fuzzy PID-type sliding mode control (AFPID-SMC) is designed with the utilizationof radial basis function (RBF) neural network. In order to improve the transient performance and ensure smallsteady state tracking error, the gain parameters of PID-type sliding mode manifold are adjusted online by usingadaptive fuzzy logic system (FLS). Additionally, the designed new adaptive law can ensure that the closed-loopsystem is asymptotically stable. Meanwhile, the problem of the actuator saturation, caused by integral term ofsliding mode manifold, is avoided even under large initial tracking error. Furthermore, to eliminate the need of apriori knowledge of the disturbance upper bound, RBF neural network observer is used to estimate the disturbanceinformation. The stability of the closed-loop system is proved via Lyapunov direct approach. Finally, the numericalsimulations verify that the proposed controller is better than conventional PID-type SMC in terms of improving thetransient performance and robustness.

Characterization and performance of SmxA1xMnO3 (A=Ce, Sr, Ca) perovskite for efficient catalytic oxidation of toluene

Jinghui Yao,Jiabin Zhou,Tianlei Zhang,Su Liu,Ke Du 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.11

Catalytic oxidation of toluene was implemented over SmMnO3, Sm0.8A0.2MnO3 (ABO3, A=Ce, Sr, Ca) and Sm1xCaxMnO3 (x=0.0, 0.1, 0.2, 0.3) perovskite oxides synthesized via sol-gel method. The effects of A-site substitution of SmMnO3 and the amount of calcium substitution of SmMnO3 perovskite-type catalyst on the catalytic activity of toluene were investigated in a fixed bed reactor. The structure and chemical properties of the perovskites were studied by XRD, SEM, XPS, and H2-TPR. The results showed that the substitution of Ce and Ca had a positive impact about the catalytic properties of toluene oxidation, while a negative impact was caused by the substitution of Sr. The catalytic activity of toluene oxidation followed the order of Sm0.8Ca0.2MnO3>Sm0.8Ce0.2MnO3>SmMnO3>Sm0.8Sr0.2MnO3 in terms of the temperature of T90%, at toluene concentration=1,000 ppm and weight hourly space velocity (WHSV)=3,000mL/ g·h. Sm0.8Ca0.2MnO3 had the highest catalytic capacity (T90%=238 oC), which could be attributed to its high adsorbed oxygen concentration, Mn4+/Mn3+, and the best low-temperature reducibility (H2 consumption=0.36). Meanwhile, the Sm0.8Ca0.2MnO3 catalysts showed great long-term stability after 30 h of the reaction, and the toluene degradation rate remained over 95% at 350 oC.

Structural Directed Growth of Ultrathin Parallel Birnessite on β-MnO₂ for High-Performance Asymmetric Supercapacitors

Zhu, Shijin,Li, Li,Liu, Jiabin,Wang, Hongtao,Wang, Tian,Zhang, Yuxin,Zhang, Lili,Ruoff, Rodney S.,Dong, Fan American Chemical Society 2018 ACS NANO Vol.12 No.2

<P>Two-dimensional birnessite has attracted attention for electrochemical energy storage because of the presence of redox active Mn<SUP>4+</SUP>/Mn<SUP>3+</SUP> ions and spacious interlayer channels available for ions diffusion. However, current strategies are largely limited to enhancing the electrical conductivity of birnessite. One key limitation affecting the electrochemical properties of birnessite is the poor utilization of the MnO<SUB>6</SUB> unit. Here, we assemble β-MnO<SUB>2</SUB>/birnessite core–shell structure that exploits the exposed crystal face of β-MnO<SUB>2</SUB> as the core and ultrathin birnessite sheets that have the structure advantage to enhance the utilization efficiency of the Mn from the bulk. Our birnessite that has sheets parallel to each other is found to have unusual crystal structure with interlayer spacing, Mn(III)/Mn(IV) ratio and the content of the balancing cations differing from that of the common birnessite. The substrate directed growth mechanism is carefully investigated. The as-prepared core–shell nanostructures enhance the exposed surface area of birnessite and achieve high electrochemical performances (for example, 657 F g<SUP>–1</SUP> in 1 M Na<SUB>2</SUB>SO<SUB>4</SUB> electrolyte based on the weight of parallel birnessite) and excellent rate capability over a potential window of up to 1.2 V. This strategy opens avenues for fundamental studies of birnessite and its properties and suggests the possibility of its use in energy storage and other applications. The potential window of an asymmetric supercapacitor that was assembled with this material can be enlarged to 2.2 V (in aqueous electrolyte) with a good cycling ability.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2018/ancac3.2018.12.issue-2/acsnano.7b03431/production/images/medium/nn-2017-03431b_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn7b03431'>ACS Electronic Supporting Info</A></P>

Composite Behavior of Sandwich Panels with W-Shaped SGFRP Connectors

Huanzhi Jiang,Zhengxing Guo,Jiabin Liu 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.5

The composite behavior and structural behavior of four full-scale precast concrete sandwich panels (PCSPs) with different numberand distribution of W-shaped steel glass fiber reinforced polymer (SGFRP) shear connectors were investigated both experimentallyand theoretically in this paper. Test results showed that the number of W-shaped SGFRP connectors in the panel ends a third ratherthan in the middle section has a significant impact on the flexural capacity and the composite action of PCSPs. The ultimate loadincreased in proportion to the number of W-Connectors in both panel ends a third approximately. The shear connectors could transfera large level of shear from one wythe to the other to enable the two concrete wythes to exhibit partially composite behavior. Thedegree of partially composite action of the tested PCSPs achieved obviously different depending on the number of connectors in theends a third. Good correlation was found between experimental obtained results and the theoretical calculation results of the degreeof composite action.

The Shear Behavior of Precast Concrete Sandwich Panels with W-shaped SGFRP Shear Connectors

Huanzhi Jiang,Zhengxing Guo,Jiabin Liu,Hui Liu 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.10

This paper presents a horizontal direct shear push-out test program to evaluate the shear behavior of Precast Concrete Sandwich Panel (PCSP) systems with W-shaped Steel Glass Fiber-reinforced Polymer (SGFRP) shear connectors. The experimental program is composed of twelve PCSPs reinforced with W-shaped SGFRP connectors and six PCSPs for comparison that were panels using pure W-shaped GFRP connectors, GFRP pin connectors and stainless-steel truss connectors. The peak shear load, the relationships of shear force-average relative slip, the shear performance of each connector, and the effects of different height, angle and diameter of the SGFRP connector were investigated in this paper. The test results indicate that all specimens with the W-shaped SGFRP shear connectors in this study exhibited an elastic-brittle response caused by pull-out of certain connectors before the ultimate strength was utilized fully and the ductility of the SGFRP material did not play out. Nevertheless, the shear capacity of panels with SGFRP connectors was approximately 2 times that with pure W-shaped GFRP connectors in the case of all parameters being the same, approximately 2 times that with stainless-steel truss connectors for which diameter was reduced by a half, and approximately 2.5 times that with GFRP pin connectors. A reasonable design of connector diameter, spacing and measure of strengthened anchorage is required to ensure the ductile failure of panels.