정상과 갑상선 종양조직에서 사람 IGF-I 유전자의 발현

김성운,장현하,박상미,김덕윤,우정택,양인명,김진우,김영설,김광원,고석환,홍성화,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

Many of the growth-promoting properties of growth hormone(GH) are mediated by insulin-like growth factor-I(IGF-I), a highly conserved circulating 70-amino acid peptide. Recent studies have shown that multiple mechanisms influence IGF-I gene expression, including transcription from two promoters, alternative RNA splicing, and variable polyadenylation. In thyroid tissue, thyroid stimulating hormone(TSH) and IGF-I are the most possible candidates for follicular cell proliferation and hypertrophy. Actually IGF-I had autocrine and paracrine effect for tissue growing. We prepared thyroid tumor tissue mRNAs using single step method for detecting IGF-I levels according to different tissues, i.e., thyroid adenoma or papillary thyroid carcinoma. We used Northern blot analysis for IGF-I mRNA and RNase protection assay (RPA) for IGF-I transcription start sites. For Northern blot, we used whole human IGF-I cDNA as a DNA probe and for RPA, we used IGF-I exon 1 containing noncoding promoter 1 as a riboprobe. We got good RNA bands from Northern blot analysis around 1 kb (IGF-IA) and 7.5 kb (IGF-IB) region. To clarify the amount of both IGF-IA and IB mRNAs, we measured autoradiographied signal of IGF-I mRNAs bands using densitometer. In IGF-IA signals, there's no change among liver and thyroid tissues, but in case of IGF-IB mRNA bands, the signal was markedly increased in thyroid carcinoma tissues than that of normal thyroid tissue (85% vs 14%). In the study of RPA, all thyroid tissues used the same transcription start sites as those of liver's. We concluded that that this different regulation of IGF-I mRNA was originated from tissue specificity. That meant some tissue specific transcription factor/s were related to tissue IGF-I expression.

임상간호사의 사회심리적 건강과 건강지각, 건강관련 행위

김정희,현미열,김영순,김정실,남상옥,송만숙,이라주,이정화 한국간호과학회 간호행정학회 2010 간호행정학회지 Vol.16 No.1

Purpose: The purpose of this study was to investigate the relationships among psycho-social well-being, and health perception and behavior in clinical nurses. Methods: The data were collected from 362 nurses employed at five general hospitals. A self-reported questionnaire was used to assess demographics and job characteristics, health perception and behavior, depression, and psycho-social stress. The data were analysed using Cronbach's alpha, descriptive statistics, t-test, and ANOVA. Results: While nurses perceived themselves to be in good health, represented by a high mean for health perceptions, actual health behavior was poor as the mean for health behavior was low. Forty two percents of the subjects were categorized as having major depression and high-stress levels. There were statistically significant differences in psycho-social well-being according to age, marital status, years of employment, and monthly salary. Also, there were significant differences in psycho-social well-being according to health perception, regular meals and exercise, sufficient sleep, job related injury and accident, and absences for ill. Conclusion: In order to prevent depression and improve psycho-social well-being among clinical nurses, organizational support and health management needs to be focused on high-risk nurses. Furthermore, it is essential to consider the health perception and behavior of clinical nurses.

昆蟲의 酵素分析에 의한 環境汚染比較에 관한 硏究

金正和,李仁煥,李容揆 충북대학교 농업과학기술연구소 1992 農業科學硏究 Vol.10 No.1

The experiment was conducted to elucidate the differences of several mosquito strains(Culex pipiens pallans Coquillet : Mushimcheon, Daecheongdam, Oksan, Weolacksan) to various insecticides using the methods such as bioassay, electrophoresis, and filter paper test. And the results are summarized as follows. 1. In LC50, Mushimcheon strain showed the highest insecticidal resistance while that of Weolacksan strain was the lowest. Resistant ratio(RR50) of mosquito srains to temephos was very high while all strains showed very low rate to propoxur. In LC95, insecticidal resistance to temephos was very high in Oksan strain while Daecheongdam strain was very low. 2. Est-Bl was detected in all strains tested by electrophoresis. And the activity was very high in Oksan strain and very low in Daecheongdam strain. 3. Esterase activity, determined by filter paper test and digital densitometry, was very high in Oksan strain and that of Daecheongdam was very low. 4. The results obtained thus far indicated that the mosquito strains that inhabit in Oksan and Mushimcheon region where respective agricultural chemicals and insecticides to control medical insects are heavily applicated are more contaminated by insecticidal chemicals than other regions.

合成洗劑가 빨간집모기의 生態, 生理學的 特性에 미치는 影響

金正和,李炯來,朴垠澈,李容揆,崔觀善 충북대학교 농업과학기술연구소 1995 農業科學硏究 Vol.12 No.2

It is considered a general experience that migration from rural to urban areas will go on in any developing countries pursuing economic development through industralization. In Korea, migration is considered one of the serious socio- economic problems because it has been made excessively fast than any other countries' experiences. The socio- economic problems originates in migration can be classified in two ways ; the one is the overpopulated problems of urban areas and emptied villages problems of rural areas, and the other is the shartage agricultural labor farce problems symbolized by the increasing idle cultivated land. To relieve migration effectively, the developing policies should be converted from pursuing fast industralization and urbanization to balance growth between areas and industries. Moreover problem solving political measure should be converted from partial and corresponding approaches to total approaches containing economic, social and institutional side-view.

감꼭지나방(나비목:감꼭지나방과)의 발육생태

김정화,김길하,조수원,박은철,박형진 충북대학교 첨단원예기술개발연구센터 2000 연구보고서 Vol.5 No.-

충북 영동의 감나무 가로수에서 감꼭지나방(Stathmopoda masinissa)을 채집하여 온도 25±1℃,광주기 16L:8D, 상대습도 85%의 실내조건에서 이들의 발육생태를 조사하였다. 각 충태별 발육일수는 알기간 7.4일, 유충기간 34.8일, 번데기기간이 15.5일이었다. 1령에서 5령의 영기간은 각각 3.5일, 4.2일, 5.2일, 6.5일, 15.4일이었으며, 유충의 두폭은 각각 0.20, 0.40, 0.65, 0.87, 1.07mm이었다. 용화율과 우화율은 각각 68.0%, 59.9%이었다. 성충 수명은 수컷이 6.2일, 암컷이 10.1일이며, 암컷 한 마리당 평균 산란수는 24.4개였다. Persimmon fruit moth, Sathmopoda masinissa, collected from roadside persimmon trees planted as shade trees in yeongdong area was used to investigate their developmental ecology under laboratory condition; 25±1℃, 16L : 8D and 85% RH. Developmental periods for eggs, larvae, and pupae were 7.4, 34.8 and 15.5 days, respectively. The duration for each larval instar from the 1st to the 5th was 3.5, 4.2, 5.2, 6.5 and 15.4 days, respectively, and the head capsule width of corresponding stage was 0.20, 0.40, 0.65, 0.87 and 1.07mm. Rates of pupation and emergence were 68.0 and 59.9%, respectively. Longevity of adult males was 6.3 days while that of females was 10.1 days. Average fecundity was 25.4 eggs.

농촌에 서식하는 빨간집모기의 살충제 저항성에 대하여

金正和,朴垠澈 충북대학교 농업과학기술연구소 1996 農業科學硏究 Vol.13 No.-

The expriments were carried out elucidate the different resistance of Temephos and Dichlorvos against the Mosquito, Culex pipiens pallens, collected in a rural community using various methods such as bioassay, electrophoresis and filter paper test. The experimntal results were as following 1 . Bioassay The LC50 and LC95 Values of each strains of the mosquito were determined using profit analysis. Insecticide resistance of each strains was collected from July to September in 1995. Resistant ratio(RR50) of cowshed strain showed 1.03 and 0.44 times to the Temephos and Dichlorvos treated. Resistant ratio(RR50) of henhouse strain showed 2.14 and 0.93 times to the temephos and Dichlorvos treated. Resistant ratio(RR 50) of pigpen strain showed 1.46 and 1.28 times to the Femephos and Dichlorvos treated. 2. Enzyme activity 1) Esterase isozyme pattern Est-αaβ1 was detected in all strains tested by elecgrophorsis. The activity was the highest in cowshed strain and the lowest in pigpen strain. 2) Filter paper test Esterase-α activities showed higher 1.41 and 1.40 times to the cowshed and heenhouse strain treated than that of the control, respectively, by filter paper test.

비수용 혼합용매에서 Benzoylcyanide 의 가용매분해 반응

김정화 晋州敎育大學校 科學敎育硏究所 1986 科學敎育硏究 Vol.12 No.-

비수용인 에탄올-아세톤, 에탄올-아세토니트릴 혼합용매 그리고 메탄올-아세톤,메탄올-아세토니트릴 혼합용매계에서 시안화벤 조일의 가용매분해반응에 대한 유사 일차반응속도상수를 25˚C, 30˚C, 40˚C에서 용매조성변화에 따라 구하였다. Grunwald-winstein plot의 m 값 Kivinen Plotdml n값, Leffer 관계식의 β값을 구하여 용매변화에 따른 전이상태변화를 논의하였고, 각 혼합용매계에 에탄올, 메탄올의 몰분율에 입각한 용매효과를 검토 하였다. 본 반응은 dissociative S?2 반응 메카니즘으로 진행됨을 알 수 있었다. The pseudo-first order rate constants have been obtained for the solvolysis of benzoylcyanide in various nonaqueous binary mixtures, i,e. EtOH-(Me)2CO, EtOH-MeCN,MeOH-(Me)2CO, MeOH-MeCN, by varing the composition of solvent at 25,30,35 and 40˚C. Values of m in Grunwald-Winstein plot, n values of Kivinen plot,βvalues of Leffler relationship have been calculated and studied the transition state variation caused by solvent change, and solvent effect of ethanolysis and methanolysis has been investigated in terms of mol fraction concentration. It has been shown that the reaction proceed vea ther dissociative Sn2 mechanism,using the transition state parameters.

금잔화 잎 추출물의 살충활성과 생리적 특성에 관한 연구

김정화,박은철 충북대학교 농업과학기술연구소 1998 農業科學硏究 Vol.15 No.-

The experiment was carried out to elucidate insecticidal activity and physiological characteristics of the extrac from leaves of pot marigold (Calendula arvensis L.) against cockroach (Blattella germanica L. ), house fly (Musca demestica L. ), mosquito (Culex pipiens pallens Coquillett), brown planthopper (Nilaparvata lugens Stal) and diamondback moth (Plutella xylostella L.). Each extract was tested by various bioassays, esterase isozyme pattern and esterase activity. The results obtained from the experiment was summarized as follows. 1.Bioassay The extract from leaves of pot marigold showed insecticidal activity against all insect pests. The insectcidal activity against insect pests showed 43, 63 and 81% in the 1,000, 5,000 and 10,000 ppm against cockroach, 45, 65 and 78% in the 1,000, 5,000 and 10,000 ppm against mosquito, 43% in the 10,000 ppm against house fly, 57, 67 and 87% against brown planthopper and 43, 50, 58 and 73% in the 100, 1,000, 5,000 and 10,000 ppm against diamondback moth. 2.Esterase isozyme pattern Esterase isozymes were detected in the extract from leaves of pot marigold against insect pests by agarose gel electrophoresis. Esterase isozymes of cockroach showed the bands of Est-α1β1, Est- α2β2, Est-α1, Est-α2 and Est-α3. Est-α1β1, Est- α2β2, Est-α3 in the control, 0.1 and 5,000 ppm, Est-α1, Est-α2 and Est-α3 in the 1, 10, 100 and 1,000 ppm, Est-α1, Est-α2 and Est-α3 in the 10,000 ppm were shown, respectively. Esterase isozyme of mosquito showed the band of Est-β1 in the all concentration. Esterase isozyme of house fly showed the bands of Est-α1, Est-α2 Est-α3 and Est-α4 in the control, 0.1, 1, 10, 100, 1,000 and 5,000 ppm, Est-α1, Est-α2 and Est-α3 in the 10,000 ppm. Esterase isozyme of brown planthopper showed the band of Est-β1 in the all concentration. Esterase isozyme of diamondback moth showed the bands of Est-α1, Est-α2 Est-α3 and Est-α4. Est-α1, Est-α2 Est-α3 and Est-α4 in the control, 0.1, 1, 10, 100, and 1,000 ppm, Est-α1, Est-α2 in the 5,000 ppm and Est-α1 in the 10,000 ppm were detected. 3.Filter paper test Esterase-α activities of insect pests against the extract of pot marigold leaves were examined by the filter paper test. Esterase-α activities of cockroach showed 0.98, 0.76, 0.70, 0.57, 0.51, 0.49 and 0.43 times that in the control, 0.1, 1, 10, 100, 1,000, 5,000 and 10,000 ppm, respectively. Esterase-α activities of mosquito showed the 0.41, 0.31, 0.36, 0.23, 0.18, 0.14 and 0.14 times that in the control, 0.1, 1, 10, 100, 1,000, 5,000 and 10,000 ppm, respectively. Esterase-α activities of House fly showed the 1.30, 0.80, 0.75, 0.65, 0.40, 0.35 and 0.25 times that in the control, 0.1, 1, 10, 100, 1,000, 5000 and 10,000 ppm, respectively. Esterase-α activities of brown planthopper showed the 0.94, 0.81, 0.64, 0.58, 0.42, 0.31 and 0.25 times that in the control, 0.1, 1, 10, 100, 1,000, 5,000 and 10,000 ppm, respectively. Esterase-α activities of diamondback moth showed the 0.90, 0.85, 0.62, 0.72, 0.50, 0.27 and 0.22 times that in the control, 0.1, 1, 10, 100, 1,000, 5,000 and 10,000 ppm respectively.

Clinoptilolite 계열의 국내산 천연제올라이트의 상규명 및 NH₄^+ : 이온교환능에 관한 연구

김화중,이기정,이명철 건국대학교 1995 學術誌 Vol.39 No.2

Domestic natural zeolite, Clinoptilolite series was investigated to identify the phase and to study the capability of NH4+-ion removal from the solution system. The domestic natural zeolite mined from Young-II man area was proven to be a thermally unstable zeolite, heulandite by XRD and FT-IR analyses. In addition, the heulandite exchanged by K+ ion showed the highest thermal stability upon the heat-treatments. However, the best capability of removing NH4+-ion from the solution system was the Na+-exchanged zeolite.

서열 환경에서 농작업 모자 착용에 따른 체온 조절 및 주관적 반응

김명주,최정화 한국의류학회 2004 한국의류학회지 Vol.28 No.5

This study examined the effects of two kinds of functional sun hats through a head-manikin test a climatic chamber trial for farm workers in summer. Experiment was composed of four conditions. The first condition without any hat(Control). The second was the condition with a sun hat on the market (Hat A). The third was the condition with a functional sun hat made of reflective fabric (Hat B). The last was the condition with a functional sun hat having a ventilating structure as well as reflective fabric (Hat C). For the subjects in the climatic chamber trials, 12 healthy males volunteered. Air temperature, relative humidity and globe temperature in the chamber was maintained at 33±0.5℃, 65±5%RH and 39±1℃ (WBGT 33℃). Subjects did a simulated red pepper-work (50-min work and 10-min rest, twice repetition) for 120 min. As the result of head-manikin test, the surface temperature on middle of back-neck was the lowest in Hat B of four conditions and the surface temperature on top of head was the lowest in Hat C. As the result of climatic chamber trials, there were apparent differences between with (Hat A, Hat B, Hat C) and without a sun hat (Control). In rectal temperature (T_(re)), mean skin temperature (T_(sk)), heart rate (HR), total sweat rate (TSR), The physiological heat strain was less in the condition with hats than is the condition without a sun hat. As the increasing rate in Tre, Hat B is the most effective hat for alleviation heat strain. As the subjective responses, Hat B was the most effective hat for thermal comfort even though the difference was not significant. Hat C was less effective than Hat B and the reason might be the increase of weight due to inserting the ventilating structure.