A genetic variation in microRNA target site of <i>KRT81</i> gene is associated with survival in early-stage non-small-cell lung cancer

Lee, S. Y.,Choi, J. E.,Jeon, H. S.,Hong, M. J.,Choi, Y. Y.,Kang, H. G.,Yoo, S. S.,Lee, E. B.,Jeong, J. Y.,Lee, W. K.,Lee, J.,Cha, S. I.,Kim, C. H.,Kim, Y. T.,Jheon, S.,Son, J. W.,Park, J. Y. Oxford University Press 2015 ANNALS OF ONCOLOGY Vol.26 No.6

<P>In this study, <I>KRT81</I> rs3660G>C was associated with survival of patients with NSCLC after surgical resection. Mechanistic study suggested that the G-to-C change caused reduced binding efficiency of miRNA, leading to decreased translational repression, thereby increased <I>KRT81</I> expression. The <I>KRT81</I> rs3660G>C may be a useful prognostic biomarker in early-stage NSCLC patients.</P><P><B>Background</B></P><P>MicroRNAs (miRNAs) have a key role in carcinogenesis through negative regulation of their target genes. Therefore, genetic variations in miRNAs or their target sites may affect miRNA–mRNA interactions, thereby result in altered expression of target genes. This study was conducted to investigate the associations between single-nucleotide polymorphisms (SNP) located in the miRNA target sites (poly-miRTSs) and survival of patients with early-stage non-small-cell lung cancer (NSCLC).</P><P><B>Methods</B></P><P>Using public SNP database and miRNA target sites prediction program, 354 poly-miRTSs were selected for genotyping. Among these, 154 SNPs applicable to Sequenom's MassARRAY platform were investigated in 357 patients. A replication study was carried out on an independent patient population (<I>n</I> = 479). <I>Renilla</I> luciferase assay and reverse transcription-polymerase chain reaction were conducted to examine functional relevance of potentially functional poly-miRTSs.</P><P><B>Results</B></P><P>Of the 154 SNPs analyzed in a discovery set, 14 SNPs were significantly associated with survival outcomes. Among these, <I>KRT81</I> rs3660G>C was found to be associated with survival outcomes in the validation cohort. In the combined analysis, patients with the rs3660 GC + CC genotype had a significantly better overall survival compared with those with GG genotype [adjusted hazard ratio (aHR) for OS, 0.65; 95% confidence interval (CI) 0.50–0.85; <I>P</I> = 0.001]. An increased expression of the reporter gene for the C allele of rs3660 compared with the G allele was observed by luciferase assay. Consistently, the C allele was associated with higher relative expression level of <I>KRT81</I> in tumor tissues.</P><P><B>Conclusion</B></P><P>The rs3660G>C affects KRT81 expression and thus influences survival in early-stage NSCLC. The analysis of the rs3660G>C polymorphism may be useful to identify patients at high risk of a poor disease outcome.</P>

G-code control 을 이용한 웹 기반 금형 가공 공정 모니터링 & 컨트롤 시스템 개발

김건희(G. H. Kim),최진화(J. H. Choi),전병철(B. C. Jeon),조명우(M. W. Cho) 한국정밀공학회 2004 한국정밀공학회 학술발표대회 논문집 Vol.2004 No.10월

The target of this paper is the development of Web-based monitoring & control system which is for effective and economic management of mold manufacturing process. This system has three module; G-code Control, Monitoring Module and Result analysis module. Also, as the environment of development is based on internet, this system which is possible to the remote site management of manufacturing process works on Web. To be possible to control the manufacturing monitoring by client, each module is made ActiveX control and is based on socket communication. This system makes the foundation which is possible to manage the mold manufacturing process efficiently from remote site by matching real-time monitoring with manufacturing process in factory using G-code control and displaying the result of manufacturing using Ch-CGI.

Impact of series resistance on the operation of junctionless transistors

Jeon, D.Y.,Park, S.J.,Mouis, M.,Barraud, S.,Kim, G.T.,Ghibaudo, G. Pergamon Press ; Elsevier Science Ltd 2017 Solid-state electronics Vol.129 No.-

Transconductance (g<SUB>m</SUB>) and its derivative (dg<SUB>m</SUB>/dV<SUB>g</SUB>) of junctionless transistors (JLTs), considered as a possible candidate for future CMOS technology, show their unique operation properties such as bulk neutral and surface accumulation conduction. However, source/drain series resistance (R<SUB>sd</SUB>) causes significant degradation of intrinsic g<SUB>m</SUB> and dg<SUB>m</SUB>/dV<SUB>g</SUB> behavior in JLTs. In this letter, the R<SUB>sd</SUB> effects on the operation of JLTs were investigated in detail and also verified with analytical modeling equations. This work provides helpful information for a better understanding of the operation mechanism of JLTs with de-embedded R<SUB>sd</SUB> effects.

Potential Effect of S-Nitrosylated Protein Disulfide Isomerase on Mutant SOD1 Aggregation and Neuronal Cell Death in Amyotrophic Lateral Sclerosis

Jeon, G. S.,Nakamura, T.,Lee, J. S.,Choi, W. J.,Ahn, S. W.,Lee, K. W.,Sung, J. J.,Lipton, S. A. HUMANA PRESS INC 2014 Molecular neurobiology Vol.49 No.2

Aggregation of misfolded protein and resultant intracellular inclusion body formation are common hallmarks of mutant superoxide dismutase (mSOD1)-linked familial amyotrophic lateral sclerosis (FALS) and have been associated with the selective neuronal death. Protein disulfide isomerase (PDI) represents a family of enzymatic chaperones that can fold nascent and aberrant proteins in the endoplasmic reticulum (ER) lumen. Recently, our group found that S-nitrosylated PDI could contribute to protein misfolding and subsequent neuronal cell death. However, the exact role of PDI in the pathogenesis of ALS remains unclear. In this study, we propose that PDI attenuates aggregation of mutant/misfolded SOD1 and resultant neurotoxicity associated with ER stress. ER stress resulting in PDI dysfunction therefore provides a mechanistic link between deficits in molecular chaperones, accumulation of misfolded proteins, and neuronal death in neurodegenerative diseases. In contrast, S-nitrosylation of PDI inhibits its activity, increases mSOD1 aggregation, and increases neuronal cell death. Specifically, our data show that S-nitrosylation abrogates PDI-mediated attenuation of neuronal cell death triggered by thapsigargin. Biotin switch assays demonstrate S-nitrosylated PDI both in the spinal cords of SOD1 (G93A) mice and human patients with sporadic ALS. Therefore, denitrosylation of PDI may have therapeutic implications. Taken together, our results suggest a novel strategy involving PDI as a therapy to prevent mSOD1 aggregation and neuronal degeneration. Moreover, the data demonstrate that inactivation of PDI by S-nitrosylation occurs in both mSOD1-linked and sporadic forms of ALS in humans as well as mice.

Neuroprotective Effect of Human Adipose Stem Cell-Derived Extract in Amyotrophic Lateral Sclerosis

Jeon, G. S.,Im, W.,Shim, Y. M.,Lee, M.,Kim, M. J.,Hong, Y. H.,Seong, S. Y.,Kim, M.,Sung, J. J. Springer Science + Business Media 2016 Neurochem Res Vol.41 No.4

<P>Amyotrophic lateral sclerosis (ALS) is a devastating human neurodegenerative disease. The precise pathogenic mechanisms of the disease remain uncertain, and as of yet, there is no effective cure. Human adipose stem cells (hASC) can be easily obtained during operative procedures. hASC have a clinically feasible potential to treat neurodegenerative disorders, since cytosolic extract of hASC contain a number of essential neurotrophic factors. In this study, we investigated effects of hASC extract on the SOD1 G93A mouse model of ALS and in vitro test. Administration of hASC extract improved motor function and prolonged the time until symptom onset, rotarod failure, and death in ALS mice. In the hASC extracts group, choline acetyltransferase immunostaining in the ventral horn of the lumbar spinal cord showed a large number of motor neurons, suggesting normal morphology. The neuroprotective effect of hASC extract in ALS mice was also suggested by western blot analysis of spinal cord extract from ALS mice and in vitro test. hASC extract treatment significantly increased expression of p-Akt, p-CREB, and PGC-1 alpha in SOD1 G93A mouse model and in vitro test. Our results indicated that hASC extract reduced apoptotic cell death and recovered mutant SOD1-induced mitochondrial dysfunction. Moreover, hASC extract reduced mitochondrial membrane potential. In conclusion, we have demonstrated, for the first time, that hASC extract exert a potential therapeutic action in the SOD1 G93A mouse model of ALS and in vitro test. These findings suggest that hASC hold promise as a novel therapeutic strategy for treating ALS.</P>

X-Y 테이블에 사용되는 볼 스크류의 열팽창 억제에 관한 연구

전언찬,이승수,김민주,곽창식,장성규,한근조,김중완,박홍식,전태옥,박정보 동아대학교 공과대학부설 생산기술연구소 1997 生産技術硏究所硏究論文集 Vol.2 No.2

In this paper, we have studied about the thermal expansion of the ball screw used for the X-Y table. The hollow ball type is used for the vall screw. We have compared the conventional cooling system and function with the improved cooling system and function which is developed the path providing cooling oil in hollow ball screw. That is, the temperature variation and positioning accuracy are analyzed of the ball screw. We have obtained the following result through this experiment. 1) The improved cooling system of the hollow ball screw for X-Y table was developed. 2) The improved cooling system of the hollow ball screw has a large effectiveness on restraining the thermal expansion of the ball screw. 3) The positioning accuracy of the ball screw was improved about 2∼4㎛ using temperature-controlled colling oil.

한우 경제형질에 미치는 Mitochondrial DNA D-loop 영역의 염기서열 변이효과

오재돈,윤두학,공홍식,임현진,이학교,조병욱,홍기창,전광주 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.6

This study was performed to analyse the sequences of variations of mtDNA D-loop and their effects on carcass traits in Hnawoo(Korean cattle). The resulting sequences were compared with perviously published sequences for other cattle breeds(GenBank J01394). The PCR was used to amplify a total of 964 bp between nucleotide 15758 and 383 within D-loop region of mtDNA using specific primers. Twenty five polymorphic sites by nucleotide substitution were found in mtDNA of Hanwoo. The frequencies of positions at 169, 16042, 16093. 16119, 16255 and 16302 nt with high levels of sequence polymorphism were 0.891, 0.117, 0.109, 0.182, 0.197 and 0.117, respectively. The substitution effect at 169 and 16119 nt was found significant on marbling score. Also substitution effect at 169 and 16042 nt was highly significant(p<0.01) on backfat, thickness. Polymorphisrn of mtDNA sequence in D-loop region could be useful for the analysis of cytoplasmic genetic variation and associations with the other economically important traits and maternal lineage analysis in Hanwoo.

Electric Probe Measurements at Edge Region During H‐Mode Discharges in KSTAR

Bak, J.G.,Oh, Y.S.,Kim, H.S.,Hahn, S.H.,Yoon, S.W.,Jeon, Y.M.,Xiao, W.W.,Ko, W.H.,Kim, W.C.,Kwak, J.G.,Woo, H.J.,Chung, K.S.,the KSTAR project team, WILEY‐VCH Verlag 2013 Contributions to plasma physics Vol.53 No.1

<P><B>Abstract</B></P><P>Electrical probe measurements are carried out at the scrape‐off‐layer (SOL) and divertor regions in order to investigate the characteristics of edge plasmas during H‐mode discharges in the Korea Superconducting Tokamak Advanced Research (KSTAR) device. Radial profiles of plasma parameters such as electron temperature T<SUB><I>e</I></SUB>, plasma density n<SUB><I>e</I></SUB>, and parallel flow velocity v<I><SUB>‖</SUB></I> are measured by using a fast reciprocating Langmuir probe assembly (FRLPA) at the SOL region. From the FRLPA measurements, it is found that the decay length of temperature λ<I><SUB>Te</SUB></I> and that of density λ<I><SUB>ne</SUB></I> are 2 <I>∼</I> 4 cm and 1 <I>∼</I> 3 cm, respectively. The magnitude of v<I><SUB>‖</SUB></I> near the last closed flux surface (LCFS) is 4 <I>∼</I> 15 km/s. The radial flux due to edge turbulence at the SOL region is investigated by using spectra analysis on electrostatic fluctuation levels such as ion saturation current Ĩ<I><SUB>is</SUB></I> and floating potential <TEX>$ \tilde{\rm V}_f$</TEX> obtained from the FRLPA measurement. The value of the flux is estimated as <I>∼</I> 10<SUP>20</SUP> particle m<SUP>−2</SUP> s <SUP>−1</SUP> near the LCFS. The poloidal distribution of the ion saturation current density j<I><SUB>is</SUB></I> is measured by fixed edge Langmuir probe array (ELPA) at the divertor region, and it is found that the positions of strike points from the ELPA measurement agree well with those reconstructed from the EFIT result using magnetic diagnostic data. From the spectrum analysis on the ELPA measurements at the divertor region during edge localized modes (ELMs) control in the H‐mode discharges, it is observed that the magnitude of <TEX>$ \tilde{\rm j}_{is}$</TEX>(<I>ω</I>) near strike point decreases when the ELMs are suppressed or mitigated (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)</P>

개체모형에 의한 한우의 성장단계별 체중의 유전모수 추정

최재관,전기준,이창우,나기준,이채영,김종복 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.5

The objective of this study were to investigate the genetic characteristics of body weight by growth pcriods for Hanwoo. A total of 1,736 records were used for body weight. The data for body weights were collected from 1990 to 2000 in Dackwanryon branch, National Livestock Research Institute(NLRI). Estimates of (co)variance components were obtained by derivative-free Restricted Maximum Likelihood (DF-REML). The results are summarized as follows; The means for the weights were 25.60, 79.31, 98.91, 145.40, 283.62, 392.32, 545.65㎏ at birth, 3, 4, 6, 12, 18, 24month postpartum, respectively. The effects of calving year-season were significant for the milk yield of cow. Heritability estimates of direct genetic effects for birth weight were 0.54(all), 0.52(female), 0.36(male) in model Ⅰ, 0.45(all), 0.41(female), 0.24(male) in modelⅡ, and heritabilities estimates of direct genetic effects for 4 month(weaning) weight were 0.47(all), 0.33(female), 0.28(male) in model Ⅰ, 0.38(all), 0.21(female), 0.21(male) in model Ⅱ. Heritability estimates for male and female data differed from those for combined data. The estimates became smaller for the body weights at 12 month or later(0.13~0.05). The heritabilities of average daily gain were smaller than those for body weights, but showed that the similar pattern to body weights.

Clinical Outcome of Autologous Hematopoietic Cell Transplantation in Adult Patients with Acute Myeloid Leukemia: Who May Benefit from Autologous Hematopoietic Cell Transplantation?

Yoon, J.H.,Kim, H.J.,Park, S.S.,Jeon, Y.W.,Lee, S.E.,Cho, B.S.,Eom, K.S.,Kim, Y.J.,Lee, S.,Min, C.K.,Cho, S.G.,Kim, D.W.,Lee, J.W.,Min, W.S. AMERICAN SOCIETY FOR BLOOD AND MARROW 2017 BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION Vol.23 No.4

The role of autologous hematopoietic cell transplantation (auto-HCT) for postremission therapy of acute myeloid leukemia is yet to be elucidated. We retrospectively analyzed 240 patients treated with auto-HCT in first remission. All patients were treated with standard induction chemotherapy, and CD34<SUP>+</SUP>@?stem cells were collected at each cycle of consolidation. Stem cells were infused after total body irradiation (1200 cGy), cytarabine (9 g/m<SUP>2</SUP>), and melphalan (100@?mg/m<SUP>2</SUP>). Estimated 5-year overall survival, disease-free survival (DFS), cumulative incidence of relapse (CIR), and nonrelapse mortality were 58.4%, 55.3%, 38.8%, and 5.9%, respectively. We identified that poor-risk karyotype showed very poor outcome after auto-HCT, and then analyzed 85 patients with good to intermediate-risk molecular cytogenetics with available molecular study results and markers for minimal residual disease (MRD) such as WT1 and core-binding factor (CBF) associated MRD (ie, AML1/ETO and CBFβ/MYH11). Our data identified that old age, pre-HCT markers for MRD, and high post-HCT WT1, high dose of CD34<SUP>+</SUP>@?stem cell (≥4.5 x 10<SUP>6</SUP>/kg) infusion, and c-kit or FLT3-ITD mutations were associated with higher relapse rate and poor DFS. Using pre-HCT parameters, except for post-HCT WT1, multivariate analysis revealed that patients with young age (<40 years old), no adverse mutations, and limited dose of CD34<SUP>+</SUP>@?stem cells might be good candidate for auto-HCT (3-year DFS and CIR were 83.4% and 16.6%, respectively). Young patients with good- to intermediate-risk molecular cytogenetics may benefit from auto-HCT if stem cell dose is limited.