토양으로부터 分離한 Bacteriophage의 특성

박현균,김도경,박경진,전홍성,박종필,김종승,김성준 조선대학교 부설 유전생물공학연구소 1992 유전생물공학연구지 Vol.2 No.-

환경 미생물 분야와 산업미생물 분야 및 분자생물학 분야에서 주목을 받고 있고, 많은 연구가 된 새로운 E. coli의 bacteriophage를 토양으로 부터 분리해서, 그 물리적인 특성과 핵산을 밝혀내, phage상호간의 관련성을 검토하여 보았다. 그 결과를 요약하면 다음과 같다. 1. 토양으로 부터 분리한 phage는 숙주균에 따라 SJ-1, SJ-2, SJ-3, SJ-4, SJ-5, SJ-6, SJ-7이라 명명하였다. SJ phage들은 높은 숙주특이성을 나타내 phage SJ-2, SJ一7은 단일종 숙주균에만 감염이 되었고, SJ-1, SJ-3, SJ-4, SJ-5, SJ-6은 다중독성을 나타내었다. 2. 7종류의 SJ-phage는 본 실험조건하에서는 모두 virulent phage임이 밝혀졌다. 모든 용균반의 형태는 중앙이 투명한 둥근 모양이었으며 주변에 무리가 있는 것과 없는것 두가지 형태를 나타냈다. 용균반의 크기는 1-5mm이었으며, SJ-5phage의 용균반이 5mm로서 가장 컸고 SJ-7 phage가 가장 적었다. 숙주균은 OD_600 0.3-0.5상태에서 가장 감염되기가 좋았다. 그리고 상층배지의 한천 농도와 양은 0.5%와 3ml이 가장 좋은 조건이었다. 3. 일단 증식 실험의 결과를 보면 SJ-phage는 잠복기가 15-50분이었고 평균 phage방출수는 190-250이었다. SJ-6과 SJ-7이 잠복기가 15분으로 가장 짧았고 SJ-4가 50분으로 가장 길었다. 평균 방출수는 SJ-3과 SJ-5가 250정도로 많았으며 SJ-1과 SJ-2는 약 190정도였다. 4. SJ-phage는 PD buffer에서 안정하였고, magnesium ion 5×10^-3M의 첨가는 phage에게 큰 안정성을 주었다. SJ-phage는 pH6과 pH9사이에서 안정하였으며 그 pH는 숙주균의 최적 pH였다. SJ-phage의 온도 불활성화는 40 ℃이상에서 불활성화되기 시작하여 60 ℃이상에서는 완전히 불활성화 되었다. 자외선 조사에 의한 불활성화에서 SJ-phage는 120초 이후에 완전히 불활성화 되었다. All phages used in this experiment were isolated from soil of the surburbs of Chun-Nam, and they designated as phage SJ-1, SJ-2, SJ-3, SJ-4, SJ-5, SJ-6, SJ-7. The hosts were JM105, JM109, K802, LE392, MC1000, MV1184 and PSM CC, respectively. These phages were virulent under the experimental conditions. They produced plaques with dear and round without halo. The size of plaques was 1-5mm in diameter. SJ-phages were stable in 5×10^-3M Mg^2+. Thermostability experiments indicated that SJ-phages were stable at 37℃. Optimal pH of the phages were 6-9. Exposure of the phages to U.V. for 120 seconds resulted in complete inactivation. In one-step growth experiments, the latent period at 37 ℃ was about 15-50 min and the average burst see was 190-250. Agarose gel electrophoresis of the phage DNA showed single band of high molecular weight from SJ-1 to SJ-7 phages

진주시 대기·수질·토양오염의 특성

박정호,이춘식,박현건,김형갑 진주산업대학교 1998 산업과학기술연구소보 Vol.- No.5

The research described in this paper was conducted to estimate the level of air, water and soil pollution, and plans for environmental conservation in Chinju city. The characterizations of air, water and soil pollution in Chinju were investigated during the period of September and November of 1997. The concentrations of air and soil pollutant in Chinju were lower than a metropolitan areas or environmental standard. The pollution level of water quality were higher Keong-Ho and Yang-Chen river compared with Duck-Chen river.

강진만에서 미생물의 년 변화

박종천,오재영,정용욱,주현수,이원교,유춘만,이우범 國立 麗水大學校 환경문제硏究所 2001 環境硏究論文集 Vol.4 No.-

The biological surveys were performed at seven sites in the Gangjin bay from February, 1998 to November, 2001. In order to analysis of environmental variation, numbers of heterotrophic bacteria, general fungus and fecal coliforms were enumerated in each sample. The result showed that variation range of heterotrophic bacteria, general fungus, and fecal coliforms were 2.9 x 10^(3)~9.6 x 10^(3) CFU/ml, 17.2~80.1 CFU/ml, and 6.3~682.5 CFU/100ml for four tears(1988~2001), respectively. Density of heterotrophic bacteria and general fungus were gradually decreased for four tours, while fecal coliforms were suddenly increased. Specially, numbers of fecal colifoms were increased the more 100 times in 2001 than that 1998. We think that it was probably caused a little rainfall in February, 2001. There result suggests that ecosystem of Gangjin bay were mainly regulated by 2~3 factors, which were inflow of fresh water, salinity and variation of water temperature.

Glutamate 억제제가 Pilocarpine에 의한 경련 및 기억장애에 미치는 영향

천임순,한형수,박정숙,정헌주,이만기,김중영 慶北大學校 醫科大學 1996 慶北醫大誌 Vol.37 No.3

목적 : Pilocarpine에 의한 경련 및 기억력 저하에 glutamate 수용체가 관련이 있는지 검토하였다. 대상 및 방법 : 생쥐에 pilocarpine을 투여하여 야기되는 경련 및 기억력저하가 glutamate 수용체 길항 약물인 MK-801 및 NBQX를 전처치하였을 때의 반응을 Morris Water Maze의 방법으로 관찰하였다. 결과 : MK-801과 NBQX를 전처치하고 pilocarpine을 투여하였는 바 pilocarpine에 의한 경련유발은 억제되지 않았으나, pilocarpine에 의한 reference memory와 working memory의 저하는 MK-801이나 NBQX를 처치로서 억제되었다. 결론 : Pilocarpine에 의한 경련작용에 비하여 기억력저허가 glutamte 수용체와 관련이 있는 것으로 보인다. The role of glutamate receptors in the genesis of pilocarpine-induced seizure and seizure-related memory deficit was studied using MK-801 and NBQX. After pretreatment with MK-801 or NBQX, pilocarpine was administered intraperitoneally. Both drugs had no effect on the behavioral seizure activity and onset of seizure activity. Memory was tested with reference and working memory version of Morris water maze at 20th day after pilocarpine treatment. Reference and working memory deficit was produced by pilocarpine. These deficit were prevented by MK-801 or NBQX, which were given in once a day for 4 day at 7th to 10th days after pilocarpine treatment. These results show that although MK-801 and NBQX do not have anticonvulsant property they can prevent pilocarpine-induced memory deficit.

강진만 미생물군집의 계절별 분포

박종천,주현수,이우범 國立 麗水大學校 環境問題硏究所 1999 環境硏究論文集 Vol.2 No.-

The biological and ecological surveys were carried out at six site water region from February to October. 1998, In other to analysis of environmental variation, density population of heterotrophic bacteria, general fulgi and fecal coli were determined on each sample. The result, ecosystem of Gangjin bay were regulated by 2~4 factors for four season. The results showed that distribution of the microbial population was mainly infuluenced by inflow of fresh water, salinity and variation of water temperature. And suggested that environmental factor of Gangjin bay was relatively simple than those other bays.

Osprey Forming 공정으로 제조된 고강도 저합금강의 기계적 특성에 관한 연구

박수정,최답천,이현규 조선대학교 생산기술연구소 2004 生産技術硏究 Vol.26 No.1

High strength low alloy steel was fabricated by Osprey forming process. The billet have been characterized by optical microscopy, scanning electron microscopy. hardness test. tensile test and impact test. High strength low alloy steel is bainite structure. Hardness range of billets was about 300 kgf/㎟ without a part of horizontal and vertical about spray angle. After hot rolling, aged specimen was tensile test in results, meas yield strength 858MPa, tensile strength 985MPa, and elongation ratio was 20.3%. TO absorption energies hot rolling specimen were 17.8J, 32.8J at -90℃, -50℃. energy of respectively but it was very high 92.7J at ambient temperature. after hot rolling, absorbed aged specimen were 102J.

설비 자동화를 통한 공정 개선 사례

박해천,김현우,이안섭 대한설비관리학회 2004 대한설비관리학회지 Vol.9 No.2

The purpose of this study is for a company with a great amount of product lead time to reduce the number of its workers and its product lead time by constructing an integrated system, which is formed by the introduction and development of hardware and software in the equipment line of the company. In this study, a company which had a lot of manual processes and therefore needed to stabilize the flow process and the production system for securing technical capacity, was selected. For the purpose of improving the process of the selected company, the present system was analyzed and some sectors which required process improvement were investigated. An integrated system was constructed by introducing and developing software(a CNC design program) as well as hardware(loading/unloading and pallet devices). In addition, the data before processing and the data after processing were comparatively analyzed to see if the process had valid results. The time required and the number of workers required in making the same product were respectively included in the data be-fore processing and after processing. As a method of measurement, a stopwatch method was used and the measurement was done 15 times.

有限要素法을 이용한 過壓密粘土의 壓密沈下豫測

현상락,류제천,김송만,박춘수 충남대학교 산업기술연구소 2002 산업기술연구논문집 Vol.17 No.1

Consolidation is the time-dependent settlement of soils resulting from the expulsion of water from the soil pores. When a saturated compressible clay layer is subjected to a stress increase, consolidation settlement occurs greatly. Because the hydraulic conductivity of clay is significantly small, the excess pore water pressure generated by loading gradually dissipates over a long period. The settlement caused by consolidation in clay may be several times greater than the immediate settlement. A number of researches are carried out to predict the consolidation behavior in field, however, most of researches show the discrepancies between the prediction and the field settlement data. Therefore, the estimation of consolidation rate is one of the important factors in the construcion on soft clayey deposits. In this paper, laboratory tests have analyzed to apply accurately the soil parameters that executed the plan of stage construction. This study analyzes consolidation behavior of overconsolidated clay with two-dimensional consolidation analysis program by use of the numerical method. By using this program, the time dependent consolidation behavior of soft deposits can be analyzed appropriately. Elastic and elastic-plastic finite element analysis are compared with Terzaghi's theory of one-dimensional consolidation.

정밀체성분 분석자료 및 복부지방율(WHR)과 임상검사 비교분석

박영원,최상철,이동춘,윤은희,이정화,김현숙,배경림,김은희 한국건강관리협회 1999 건협 Report Vol.2 No.-

교원 근무성적 평정제도 개선에 관한 연구

박천순,신현석 고려대학교 교육문제연구소 1996 敎育問題硏究 Vol.8 No.-