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Cytokine and Tumor Environments
Jin Tae Hong1*, Ju Kyung Song, Sun Mi Kwon, Byung Woo Ahn, Youngsoo Kim, Sang Bae Han 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.1
Cytokines are known to function as regulatory molecules that can be produced by virtually every nucleated cell type in the body, including lymphocytes, monocytes/macrophages, epithelial cells, fibroblasts, and many others. Cytokines include lymphocyte-derived factors (lymphokines), monocyte-derived factors (monokines), hematopoietic factors (colony-stimulating factors), connective tissue/growth factors, and chemotactic chemokines. Cytokines released in response to infection can affect tumor development in different ways. When exposed to infectious agents, cytokines are secreted by sentinel cells, such as macrophages and dendritic cells. These cytokines include interleukin 1 (IL-1) and tumor necrosis factor-α, as well as others, such as IL-6, IL-12, and IL-18. When released in sufficient quantities, these molecules can cause inflammation. Chronic inflammation is highly associated with tumor initiation, promotion, and progression. In this article, we review the roles and mechanisms of cytokines in tumor development.
Hong‑Jai Lee,Bo‑Young Shin,Jae‑Seung Moon,Ailyn Fadriquela,Selikem Abla Nuwormegbe,Chun‑Chang Ho,Jin‑Su Shin,Jee‑Sang Yoon,Sang‑Kyou Lee,Soo‑Ki Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3
Background Lack of universal replication system for hepatitis B virus with narrow host range and organ tropism has hampered to uncover the pathogenesis of HBV. Previously, we reported the essentiality of humoral milieu and its components toward HBV and hepatitis C virus survival/viability in vitro. Of these components, the precise role of enterohepatic humoral milieu such as bile acid (BA) on HBV cultivation in vitro and in vivo is unknown. Objective We explored whether BA, specifically taurochenodeoxycholic acid (tCDCA) would directly regulate the viral DNA and surface antigen expression of HBV in vitro, consequently rendering HBV to enter into human or murine immortalized hepatocytes, and non-hepatocytes. Result We found that higher concentration of taurochenodeoxycholic acid (tCDCA) is able to preserve the genomic stability of HBV in cell-free DMEM, showing higher the surface antigenicity than taurocholic acid (tCA). In line, we found that in vitro cell culture condition (100 μmol/L of tCDCA coupled with 1 × 108 g e/mL HBV) would be optimal for HBV entry into target cells. Using this, human (HepG2, Huh7), and rodent (Hepa1c1c7, H4-II-E) hepatoma cell lines were infected by HBV, as evidenced by the presence of HBV biomarkers (HBsAg, and HBV DNA in culture supernatant, as well as HBcAg in cell). Further, cellular entry test revealed that HBV is able to infect 12 different non-hepatic cell lines regardless of species, and organ/tissue, consequently reproducing progeny as confirmed by HBV biomarkers. Last, reinfection test showed that the progenies of HBV from immortalized HepG2, and Hepa1c1c7 cells are able to enter into each or vice versa naïve HepG2, and Hepa1c1c7 cells with or without BA. Conclusion This study demonstrates that enterohepatic humoral milieu such as BA, specifically tCDCA would directly regulate HBV DNA and its surface antigen expression in vitro, consequently rendering HBV to enter into human or murine immortalized hepatocytes, and non-hepatocytes. This is the first note to render HBV permissive to human or rodent hepatic and non-hepatic cells via sole manipulation of humoral milieu, thus establishing the platform for in vitro robust replication system of HBV.
개발사업 환경성평가에서의 토양 탄소저장량 산정방법 비교 연구
황상일 ( Sang Il Hwang ),박선환 ( Sun Hwan Park ),전영봉 ( Young Bong Jeon ),황정배 ( Joung Bae Hwang ),강선홍 ( Seon Hong Kang ),김진홍 ( Jin Hong Kim ) 한국환경영향평가학회 2014 환경영향평가 Vol.23 No.6
The objective of this study was to propose the best methodology for estimating soil organic carbon stocks during environmental assessment for development projects. We compared three methodologies which were developed by Korea Environment Corporation(2010), Korea Forest Research Institute (2006), and Jin-Hyun Jung (1998). We found that the methodology developed by Jin-Hyun Jung (1998) shows the worst performance and the methodology of Korea Forest Research Institute (2006) does not reflect a variety of soil types and land use characteristics shown in development project plans. Therefore, we propose that the methodology developed by Korea Environment Corporation (2010) is the most reasonable one because it is internationally accepted and used for local governments to make the inventory of greenhouse gases as well as to set up its reduction strategy.
Immobilization with Ketamine HCI and Tiletamine-Zolazepam in Cynomolgus Monkeys
Myung-cheol Kim,Sung-hyeok Hong,Soo-jin Lee,Young-suk Kim,Jae-il Lee 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2
with Ketamine HCl and Tiletamine-Zolazepam in Cynomolgus MonkeysJae-il Lee, Sung-hyeok Hong, Soo-jin Lee, Young-suk Kim and Myung-cheol Kim*Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, KoreaReceived March 4, 2003 / Accepted July 2, 2003J. Vet. Sci. (2003), 4(2), 187-191JOURNAL OFVeterinaryScience*Corresponding author: Myung-cheol Kim Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea Tel: +82-42-821-6757, Fax: +82-42-822-4216 E-mail: mckim@cnu.ac.kr
Park, Jinbong,Jeon, Yong-Deok,Kim, Hye-Lin,Lim, Hara,Jung, Yunu,Youn, Dong-Hyun,Jeong, Mi-Young,Kim, Hyun-Ju,Kim, Sung-Hoon,Kim, Su-Jin,Hong, Seung-Heon,Um, Jae-Young Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-
<P>Obesity has become a major health threat in developed countries. However, current medications for obesity are limited because of their adverse effects. Interest in natural products for the treatment of obesity is thus rapidly growing. Korean Medicine (KM) is characterized by the wide use of herbal formulas. However, the combination rule of herbal formulas in KM lacks experimental evidence. According to <I>Shennong's Classic of Materia Medica</I>, the earliest book of herbal medicine, <I>Veratrum nigrum</I> (VN) has antagonistic features against <I>Panax ginseng</I> (PG), and the PG-VN pair is strictly forbidden. In this study, we have shown the effects of PG, VN, and their combination on obesity in high-fat (HF) diet-induced obese mice and in 3T3-L1 cells. PG, VN, and PG-VN combination significantly reduced weight gain and the fat pad weight in HF diet-induced obese mice. They also significantly decreased lipid accumulation and the expressions of two major adipogenesis factors, PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I>, in 3T3-L1 cells. In addition, the PG-VN combination had synergistic effects compared with the mixture of extracts of PG and VN on inhibition of PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I> expressions at lower doses. These results indicate a new potential anti-obese pharmacotherapy and also provide scientific evidence supporting the usage of herbal combinations instead of mixtures in KM.</P>
Melanogenesis inhibitory pregnane glycosides from <i>Cynanchum atratum</i>
Jin, Qinghao,Han, Xiang Hua,Yun, Cheong-Yong,Lee, Chul,Lee, Jin Woo,Lee, Dongho,Lee, Mi Kyeong,Jung, Sang-Hun,Hong, Jin Tae,Kim, Youngsoo,Hwang, Bang Yeon Elsevier 2018 Bioorganic & medicinal chemistry letters Vol.28 No.7
<P><B>Abstract</B></P> <P>Bioassay-guided fractionation of the methanolic extract from the roots of <I>Cynanchum atratum</I> has resulted in the isolation of three new pregnane glycosides (<B>1</B>–<B>3</B>) along with four known compounds (<B>4</B>–<B>7</B>). Their structures were identified by analysis of the spectroscopic data including extensive 2D NMR. All of the isolates were evaluated for their potential to inhibit the melanin production in α-melanocyte stimulating hormone (α-MSH)-activated B16 melanoma cells. Of these, compounds <B>4</B>–<B>7</B> dose-dependently inhibited the melanin production with the IC<SUB>50</SUB> values ranging from 4 μM to 33 μM.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Hong, Sung-Jin,Ko, Young-Guk,Shin, Dong-Ho,Kim, Jung-Sun,Kim, Byeong-Keuk,Choi, Donghoon,Hong, Myeong-Ki,Jang, Yangsoo UNKNOWN 2017 CIRCULATION JOURNAL Vol.81 No.5
<P>Conclusions: Small vessel diameter of the target lesion or EIA was an independent predictor of procedural failure or vessel-specific complications. Small vessel diameter, particularly of the EIA, was also associated with increased TLR after successful endovascular therapy for iliac artery lesions.</P>