동진강 수계 농업용수 수질평가

엄미정(Mi Jeong Uhm),최정식(Jeong Sik Choi),한수곤(Soo Gon Han),김갑철(Kab Cheol Kim),문영훈(Young Hun Moon) 한국환경농학회 2000 한국환경농학회지 Vol.19 No.2

This study was conducted to monitor the irrigation water qualities along Dong-Jin river watershed. The water quality was surveyed at 6 sites from April to september during 1994∼1998. And the results were as follows : In July and August, water quality was better than that of any other months due to dilution with rainwater. Whereas, it became worse in April but it involved lower contents than limitted contents affected to the crop damage. Content of inorganic components was higher at Jeong-up and Won-pyeong stream. The reason for it that Jeong-up stream was deteriorated with sewage water from Jeong-up city, and Won-pyeong stream has narrow width. Water quality in upstream of Dong-Jin river, was evaluated best conditions in all sampling sites. For investigated period, water quality got worse from 1994 to 1995 but it was getting better to 1998 after 1995, especially at Jeong-up stream. The total equivalent of cation and anion was the highest at April through all months and at Jeong-up stream in sampling sites. Equivalent ratio of cation to anions(∑C/∑A) was higher at May than any other months and lower at Won-pyeong streams than any other sites. The value of most inorganic components was highly correlated with those of other components. But the value of NO₃^--N was not correlated with that of most components, and PO₄^(3-)-P was not correlated with COD, NH₄^+-N, NO₃^--N, SO₄^(2-_.

BCG를 대량 투여 받은 생쥐 가슴샘 겉질의 미세구조

고정식,안의태,박경호,우성임,한영복,경홍기 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.3

Cheek Flap을 이용한 안면부 재건

한예식,홍인식,이정윤,안희창 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.4

There are several technique for facial reconstruction using local, regional and distant flaps. Most of these techniques require multiple stages, leave poor color match, and create serious complications at donor site. Since 1959, a wide variety of defects in the eyelid and cheek have been reconstructed with Mustarde's cheek flap. This can mobilize a large area to be rotated into the defect, and effectively cover a relatively large defect with minimal distortion and with good color match. Advantage of the cheek flap include easy accessibility and operative technique. In the old age group that present most commonly with eyelid malignancies, there is sufficient laxity of the skin, and therefore, abundant tissue is available to reconstruct the lower lid. Problems which can develop with this flap include hematoma, infection, or flap necrosis early in the postoperative course. Late complications include problems resulting mainly from skin tension and gravity, causing ectropion or lateral canthal distortion. The author experienced excellent functional and esthetic result form 15 cases of facial reconstruction with Mustsarde's cheek flap. We reviewed history and surgical techniques of this flap referring the literature.

연령에 따른 개구리 피부의 형태학적 연구(Xenopus laevis)

한성수,고정식 순천향대학교 1983 논문집 Vol.6 No.4

Age differences in the skin structure have been studied in young (one year-old) and aged (five and a half years-old) frog, Xenopus laevis. Pieces of the skin were taken from the central area of the back and abdomen, fixed in Karnovsky's fixative (phosphate buffer, pH 7.3) and post-fixed in 1% osmium tetroxide (phosphat buffer, pH 7.3). The sections 1㎛ in thickness were made on a LKB-III ultramicrotome, and were stained with toluidine blue. In order to assess the general level of metabolic activities in epidermal cells, pieces of the skin from the frogs of the two age groups were incubated in amphibian physiological saline containing 50μCi/ml of 5-³H-uridine with a specific activity of 25.9 Ci/m mole (New England Nuclear). The labeling was carried out for 30minutes at 20。±1℃. Sections 1㎛ in thickness were cut on a LKB-III ultramicrotome and mounted on glass slides. The mounted slides were coated with Kodak NTB-3 unclear track emulsion in routine manner, exposed for a period of 1 or 2 weeks, and developed with Kodak D-19. The tissues were studied with light microscope. Observed results were as follow; 1. The epidermis in young frogs is made up of an average of 6.3 and 4.7 layers of epithelial cells at abdominal and back skin, respectively. 2. In aged frog, the number of epithelial cell layers at abdominal and dorsal surfaces increases to 8.8 and 5.6. 3. The thickness of the dermis(spongiosum) in aged frogs is decreased 25% on the abdominal side (from 267㎛ to 207㎛), but is increased by 11% on the dorsal side (from 275㎛ to 305㎛). 4. The nucleolar index and radioactive index, by epithelial cells are drastically reduced in aged frogs.

연령이 증가함에 따른 흰쥐 망막의 막대세포, 원뿔세포 및 바깥얼기층의 미세구조 변화

고정식,한종원,양남길,안의태 대한해부학회 1992 Anatomy & Cell Biology Vol.25 No.3

연령에 따른 개구리 피부에 대한 주사전자현미경적 연구(Xenopus laevis)

한성수,고정식 순천향대학교 1983 논문집 Vol.6 No.4

Surface structure of the abdominal skins of young (one year old) and aged (five and a half year old) frogs, Xenopus laevis, have been observed by scanning electron microscopy. Pieces of the skin were taken from the central area of the abdomen, fixed in Karnovsky's fixative (phosphate buffer, pH 7.3) and post-fixed in 1% osmium tetroxide (phosphate buffer, pH 7.3). Following fixation, the tissues were dehydrated in graded concentrations of ethanol and amyl acetate (1:1), and were followed by gradual increase in the volume of amyl acetate until a total substitution (100% amyl acetate) was achieved. Amyl acetate in the tissues were replaced with liquid CO₂, and dried in a "Polaron" critical point dryer. Blocks of tissues were oriented, mounted and attached by silver conducting paint on specimen stubs. All specimens were coated with gold in a vaccum evaporator. The samples were studied in a JSM-U3 scanning electron microscpe operated at an acceleration voltage of 15 KV. Observed results were as follow; 1. Based on the surface morphology two types of epidermal cells (Type I and Type II) are recognized. 2. Type I cells are smooth-surfaced and more numerous than type II cells that have irregular surface terrains which appear to result from collections of mucoid substances over areas of crowded microridges. 3. Both type of epithelial cells have numerous microridges of 100~110 nm throughout the surface in young frogs. 4. The microridges in aged frogs are more numerous and wider (160~170nm) than in young ones, and tend to produce variously modified appearance in aged frogs. 5. Along the periphery of epithelial cells are discrete marginal microridges which in young frogs are bridged by interconnecting cross-links.

Acriflavine-Guanosine 복합제 (AG60)의 조직내 분포에 대한 형광현미경적 연구

고정식,임수재,안의태,김진국,김상건,이경영,강종구,홍은경,정영신,유보림,한영복 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.1

一段階 耳介成形術을 利用한 小耳病 再建例

한예식,이정윤 大韓成形外科學會 1987 Archives of Plastic Surgery Vol.14 No.1

Total ear reconstrucion has been one of the most difficult problem in plastic surgeon. It required multiple procedures, prolonged hospitalization, high cost, and the final appearance of the reconstructed ear was still far from normal. In 1983, Song and co-workers studying the blood supply to the skin of the auricular area, presented another method of single stage total ear reconstruction. We operated 3 anotic patients with Song`s one stage total ear reconstruction procedure and agreed that this procedure solved some problems, especially shortening of multiple procedures, in classic Tanzer`s procedure. But, another problems were noted in Song`s procedure. 1. One stage technique will require minor secondary procedures for good final results. 2. Post auricular skin grafted area was larger than Tanzer`s procedure. 3. Definition of the cartilage framework was obtuse in early postoperative period, because the contraction of the skin graft and fixation of the skin flap were simultaneously occured. 4. This procedure may have high complication rate.

간 발생과정에 따른 Metallothionein 발현에 관한 연구

오승한,고정식,김완종 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

Metallothionein(MT) is a group of low molecular weight proteins, with a unique amino acid composition such as a large content (approximately 30%) of cysteine residues. The protein has been shown to play a physiological role in the absorption, transport, and metabolism, such as zinc, copper, and cadmium, but its actual functions are still not clear. This study was carried out to examine the expression of MT in developing or growing rat liver through immunohistochemical and RT-PCR method. Positive reactions against MT started to appear predominantly in the parenchmal cells of liver from the 13th day after gestation. Reaction products, or brown colorations, were localized at both the nucleus and the cytoplasm of the parenchymal cells. The intensity of this reaction gradually increased, and exhibited the strongest at birth. The intensity of MT staining diminished with growth, and by the 15th day after birth weak positive reaction was observed in the cells. In brief, positive reactions for MT were observed in the oval cells and the parenchymal cells during fetal stage, meanwhile they were present only in the parenchymal cells after birth. MT mRNA by RT-PCR seemed to be expressed from the 15th day after gestation till 15 days after birth and it was highest in the neonatal rats. These expression pattern showed similar to those that shown in the immunoistochemical results.The present results suggest that MT possibly contributes to parenchyma cell proliferation and differentiation through the storage or the supply of various metal ions in the developing rat liver.

분무열분해법에 의한 MgO 및 NiO첨가 Al₂o₃분체로 제조한 소결체에서의 Spinel확인

박정현,김한태,조경식 연세대학교 산업기술연구소 1991 논문집 Vol.23 No.2

Powders prepared by spray pyrolysis method are expected to have the compositional homogeneity and homogeneous distribution of additives. Pure MgO doped and NiO doped Al₂O₃ powders were synthesized by spray pyrolysis method at 1000℃. Sintered specimens were prepared by firing at 1600, 1650 and 1700℃ for 2 hrs. with their calcined powders of 1100℃ for 1 hr. The microstructure of pure Al₂O₃ sintered specimen showed large grains and grain-boundary pores, and its ralative density was about 91% even thought it was fired at 1700℃, 2hrs. 0.25 wt% MgO or 0.3 wt% NiO doped Al₂O₃ prevented discontinuous grain growth and eliminated residual pores, and allowded the specimen to densify to near a theoretical density. More MgO or NiO doping into Al₂O₃ impeded the grain growth and included many pores. According to SAD (selected area diffraction) and EDS analysis of sintered Al₂O₃bodies doped with 0.25, 5.0 wt% MgO and 0.3, 0.5 wt% NiO, the second phase Mg-Al spinel particles and Ni-Al spinel particles were observed at grain boundary and inside Al₂O₃ grains.