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남성 알코올 의존 환자의 삶의 질과 정신-사회-영적 특성과의 관계
최삼욱,나란희,김한오,최성빈,최영숙 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.5
Objectives : This study was designed to evaluate the relationship between quality of life (QOL) and psycho-socio-spiritual characteristics in male patients with alcohol dependence. Methods : The sample consisted of 109 men with alcohol dependence defined by DSM-IV criteria. We assessed QOL by the WHO QOL assessment instrument-BREF (WHOQOL) and SmithKline Beecham QOL (SBQOL). Sociodemographic and alcohol related data were collected, and 7 questionnaires were administered : MAST, BDI, STAI, Drinker Inventory of Consequences (DrInC), Scale of Social Support (SSS), Religious Beliefs and Behaviors (RBB) and Spiritual Well-being Scale (SWBS). The correlations between each QOL score and other variables were examined, and stepwise multiple regression analysis was performed. Results : The WHOQOL score positively correlated with education level, SSS (support) and RBB and negatively correlated with MAST, DrInC, BDI, STAI (trait) and SSS (conflict) scores. In stepwise regression analysis, the scores on the STAl and BDI contributed to the score on the WHOOOL. The SBQOL score correlated with the income level, and negatively correlated with BDI and STAI score. STAI score was a weak predictor of SBQOL score. Conclusion : The significant predictors of QOL in patients with alcohol dependence were psychological factors such as anxiety and depression.
Lee, Sun Young,Kim, Kwonseong,Oh, Han Bin,Hong, Jongki,Kang, Dukjin Korean Society for Mass Spectrometry 2017 Mass spectrometry letters Vol.8 No.3
In clinical diagnosis, it's well known that the abnormal level of uric acid (UA) in human body is implicated in diverse human diseases, for instance, chronic heart failure, gouty arthritis, diabetes, and so on. As a primary method, an isotope dilution mass spectrometry (IDMS) has been used to obtain the accurate quantity of UA in blood or serum and also develop the certificated reference material (CRM) so as to provide a SI-traceability to clinical laboratories. Due to the low solubility of UA in water, an ammonium hydroxide ($NH_4OH$) has been considered as a promising solvent to increase the solubility of UA that enables the preparation of both UA and its isotope standard solution for next IDMS-based absolute quantification. But, because of using this $NH_4OH$ solvent, it gives rise to the unwanted degradation of UA. In this study, we sought to optimize condition for the stability of UA in $NH_4OH$ solution by varying the mole ratios of UA to $NH_4OH$, followed by ID-LC-MRM analysis. In addition, we also inspected minutely the effect of the storage temperatures. Additionally, we also performed the quantitative analysis of UA in the KRISS serum certificated reference material (CRM, 111-01-02A) with diverse mixing ratios of UA to $NH_4OH$ and then compared those values to its certification value. Based on our experiments, adjusting the mole ratio of 1/2 ($UA/NH_4OH$) with the storage temperature of $-20^{\circ}C$ is an effective way to secure both the solubility and stability of UA in $NH_4OH$ solution for next IDMS-based quantification of UA in serum.
( Sun Young Lee ),( Kwonseong Kim ),( Han Bin Oh ),( Jongki Hong ),( Dukjin Kang ) 한국질량분석학회 2017 Mass spectrometry letters Vol.8 No.3
In clinical diagnosis, it’s well known that the abnormal level of uric acid (UA) in human body is implicated in diverse human diseases, for instance, chronic heart failure, gouty arthritis, diabetes, and so on. As a primary method, an isotope dilution mass spectrometry (IDMS) has been used to obtain the accurate quantity of UA in blood or serum and also develop the certificated reference material (CRM) so as to provide a SI-traceability to clinical laboratories. Due to the low solubility of UA in water, an ammonium hydroxide (NH<sub>4</sub>OH) has been considered as a promising solvent to increase the solubility of UA that enables the preparation of both UA and its isotope standard solution for next IDMS-based absolute quantification. But, because of using this NH<sub>4</sub>OH solvent, it gives rise to the unwanted degradation of UA. In this study, we sought to optimize condition for the stability of UA in NH<sub>4</sub>OH solution by varying the mole ratios of UA to NH<sub>4</sub>OH, followed by ID-LC-MRM analysis. In addition, we also inspected minutely the effect of the storage temperatures. Additionally, we also performed the quantitative analysis of UA in the KRISS serum certificated reference material (CRM, 111-01-02A) with diverse mixing ratios of UA to NH<sub>4</sub>OH and then compared those values to its certification value. Based on our experiments, adjusting the mole ratio of 1/2 (UA/NH<sub>4</sub>OH) with the storage temperature of -20℃ is an effective way to secure both the solubility and stability of UA in NH<sub>4</sub>OH solution for next IDMS-based quantification of UA in serum.
Oh, Han-Bin,McLafferty, Fred W. Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.3
Fragmentation efficiencies of various ‘activated-ion’ electron capture dissociation (AI-ECD) methods are compared for a model system of bovine ubiquitin 7+ cations. In AI-ECD studies, sufficient internal energy was given to protein cations prior to ECD application using IR laser radiation, collisions, blackbody radiation, or in-beam collisions, in turn. The added energy was utilized in increasing the population of the precursor ions with less intra-molecular noncovalent bonds or enhancing thermal fluctuations of the protein cations. Removal of noncovalent bonds resulted in extended structures, which are ECD friendly. Under their best conditions, a variety of activation methods showed a similar effectiveness in ECD fragmentation. In terms of the number of fragmented inter-residue bonds, IR laser/blackbody infrared radiation and ‘in-beam’ activation were almost equally efficient with ~70% sequence coverage, while collisions were less productive. In particular, ‘in-beam’ activation showed an excellent effectiveness in characterizing a pre-fractionated single kind of protein species. However, its inherent procedure did not allow for isolation of the protein cations of interest.
Oh, Han Bin,Leach, Franklin E.,Arungundram, Sailaja,Al-Mafraji, Kanar,Venot, Andre,Boons, Geert-Jan,Amster, I. Jonathan American Chemical Society 2011 Journal of the American Society for Mass Spectrome Vol.22 No.3
<P>The structural characterization of glycosaminoglycan (GAG) carbohydrates by mass spectrometry has been a long-standing analytical challenge due to the inherent heterogeneity of these biomolecules, specifically polydispersity, variability in sulfation, and hexuronic acid stereochemistry. Recent advances in tandem mass spectrometry methods employing threshold and electron-based ion activation have resulted in the ability to determine the location of the labile sulfate modification as well as assign the stereochemistry of hexuronic acid residues. To facilitate the analysis of complex electron detachment dissociation (EDD) spectra, principal component analysis (PCA) is employed to differentiate the hexuronic acid stereochemistry of four synthetic GAG epimers whose EDD spectra are nearly identical upon visual inspection. For comparison, PCA is also applied to infrared multiphoton dissociation spectra (IRMPD) of the examined epimers. To assess the applicability of multivariate methods in GAG mixture analysis, PCA is utilized to identify the relative content of two epimers in a binary mixture.</P>
Han Bin Oh,Sun Young Lee,Soojin Park,Yewon Lee,강혁,Ki-Ho Cho,Wha-Keun Ahn,이범구 대한화학회 2008 Bulletin of the Korean Chemical Society Vol.29 No.9
It was demonstrated that ECD MS can be successfully applied to Bovine ubiquitin (8.6 kDa, 76 amino acids) and Pseudomonas aeruginosa azurin (13.9 kDa, 128 amino acids, 1 disulfide bond) cations using photoelectrons emitted by irradiating a 266 nm UV laser light on the edge of an ion cyclotron resonance (ICR) cell trapping plate hole. The photoelectron ECD method cleaved protein backbone bonds as effectively as conventional ECD MS methods that employed a tungsten (W) wire or heated dispenser cathode as an electron source. This method offers the advantage of providing electrons for ECD MS without an apparent electron source. Key Words : 266 nm UV laser, Electron capture dissociation (ECD), Ubiquitin, Azurin, FTICR MS