The USP21/YY1/SNHG16 axis contributes to tumor proliferation, migration, and invasion of non-small-cell lung cancer

Pei Xu,Haibo Xiao,Qi Yang,Rui Hu,Lianyong Jiang,Rui Bi,Xueyan Jiang,Lei Wang,Ju Mei,Fangbao Ding,Jianbing Huang 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Deubiquitinases (DUBs) and noncoding RNAs have been the subjects of recent extensive studies regarding their roles in lung cancer, but the mechanisms involved are largely unknown. In our study, we used The Cancer Genome Atlas data set and bioinformatics analyses and identified USP21, a DUB, as a potential contributor to oncogenesis in nonsmall-cell lung cancer (NSCLC). We further demonstrated that USP21 was highly expressed in NSCLCs. We then conducted a series of in vitro and in vivo assays to explore the effect of USP21 on NSCLC progression and the underlying mechanism involved. USP21 promoted NSCLC cell proliferation, migration, and invasion and in vivo tumor growth by stabilizing a well-known oncogene, Yin Yang-1 (YY1), via mediating its deubiquitination. Furthermore, YY1 transcriptionally regulates the expression of SNHG16. Moreover, StarBase bioinformatics analyses predicted that miR4500 targets SNHG16 and USP21. A series of in vitro experiments indicated that SNHG16 increased the expression of USP21 through miR-4500. In summary, the USP21/YY1/SNHG16 axis plays a role in promoting the progression of NSCLC. Therefore, the USP21/YY1/SNHG16/miR-4500 axis may be a potential therapeutic target in NSCLC treatment.

A low loss low-temperature sintered ferrite-dielectric composite thick film for high-frequency application

Haibo Yang,Ying Lin,Jianfeng Zhu,Fen Wang 한국물리학회 2010 Current Applied Physics Vol.10 No.4

Dense Ni0.37Cu0.20Zn0.43Fe1.92O3.88/(Ba0.6Sr0.4)TiO3 composite thick films were prepared through screen printing method and sintered at 880 ℃. The powder XRD patterns confirm the coexistence of the two phases. The dielectric and magnetic properties are also reported. The results show that this kind of magnetic–dielectric composite thick films, possessing high permittivity and saturation magnetization, moderate dielectric tunability, and very low dielectric loss and coercivity, could be used in high-frequency communications for the capacitor–inductor integrating devices such as electromagnetic interference filters and antennas.

Fatigue property analysis of U rib-to-crossbeam connections under heavy traffic vehicle load considering in-plane shear stress

Haibo Yang,Hongliang Qian,Ping Wang 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.38 No.3

In this study, the fatigue property of U rib-to-crossbeam connections in orthotropic steel bridge (OSB) crossbeams under heavy traffic vehicle load was investigated considering the effects of in-plane shear stress. The applicability of an improved structural stress (ISS) method was validated for the fatigue behavior analysis of nonwelded arc-shaped cutout regions in multiaxial stress states. Various types of fatigue testing specimens were compared for investigating the equivalent structural stress, fatigue crack initiation positions, and failure modes with the unified standards. Furthermore, the implications of OSB crossbeams and specified loading cases are discussed with respect to the improved method. The ISS method is proven to be applicable for analyzing the fatigue property of nonwelded arc-shaped cutout regions in OSB crossbeams. The used method is essential for gaining a reliable prediction of the most likely failure modes under a specific heavy traffic vehicle load. The evaluated results using the used method are proven to be accurate with a slighter standard deviation. We obtained the trend of equivalent structural stress in arc-shaped cutout regions and validated the crack initiation positions and propagation directions by comparing them with the fatigue testing results. The implications of crossbeam spans on fatigue property are less significant than the effects of crossbeams.

Multiaxial fatigue behaviors of open-rib to crossbeam joints in orthotropic bridge structures

Haibo Yang,Hongliang Qian,Ping Wang,Pingsha Dong,Fillipo Berto 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.42 No.6

The fatigue behavior of welded open rib-to crossbeam joints (ORCJ) in orthotropic bridge structures is investigated using a traction structural stress method. The fatigue behaviors of welded open rib-to crossbeam joints have been a subject of study for decades for ensuring operational safety and future design improvement. A mesh-insensitive combination of traction structural stresses in ORCJ was obtained considering the effect of in-plane shear stress and validated by fatigue test results. The proposed method is advantageous for predicting fatigue cracks that initiate from the crossbeam cutout and propagate along the crossbeam. The investigations carried out with the proposed approach reveal that the normal structural stress decreases with the propagation of fatigue cracks, while the ratio of shear stress to normal stress increases. The effect of shear structural stress is significant for the analysis of fatigue behavior of ORCJ in multiaxial stress states

Synthesis and characterization of a K/K2CO3-based solid superbase as a catalyst in propylene dimerization

Haibo Jin,Heng Jiang,Qiwei Wang,Suohe Yang,Guohua Luo,Guangxiang He 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.2

A novel solid superbase 3%K/K2CO3 was prepared by loading metallic potassium on K2CO3. The optimized preparation conditions included a loading time of 1.5 h, loading temperature of 150 oC, loading amount of 3wt% and average carrier size of 120 μm. Under the optimum conditions, the conversion of propylene is about 60% with the selectivity of dimers 98.5% and the selectivity of 4MP1 86.3%. In addition, the superbase 3%K/K2CO3 has a base strength of H−≥37, and the concentration of basic sites of H−≥35 is approximately 0.3mmol·g−1 CAT. The microcrystal of metallic potassium was determined using X-ray diffraction (XRD) and differential scanning calorimetry (DSC). It was assumed that the oxygen species, which are adjacent to lattice defects, such as the crystalline corners, edges and vacancies of metallic potassium microcrystals, constituted the superbasic sites.

Reconstituted Fibril from Heterogenic Collagens-A New Method to Regulate Properties of Collagen Gels

Jian Yang,Haibo Wang,Lang He,Benmei Wei,Chengzhi Xu,Yuling Xu,Juntao Zhang,Sheng Li 한국고분자학회 2019 Macromolecular Research Vol.27 No.11

Heterotypic collagen fibril has long been found in the tissues of organisms, which plays an important role in the formation and function of complex structures of organisms. Inspiring by the phenomenon, scholars tried to incubate collagens from different sources into novel collagen materials in vitro, and the forming of heterogenic reconstituted collagen fibrils (RF) was often demonstrated by differential scanning calorimetry (DSC) and fluorescence quenching analysis. In this work, we used two type-I collagens from different species (bovine tendon and grasscarp fish skin) to coassemble in vitro, and verified the formation of RF from a new rheological perspective. In addition, we also investigated the assembly behavior, surface hydrophilicity and hydrophobicity, microscopic morphology and cell proliferation ability of the RF. The results showed that the assembly rate, surface properties, fibril size, viscoelastic properties of RF can be delicately regulated by the method of heterogenic collagen reconstitution. This study provides new experimental evidence for the reconstitution of heterogenic collagens, and also offers a new means for the regulation of collagen gel performance, which would help to expand the application range of collagen gel materials.

Safety Evaluation of Underground Gas Pipe under Blasting of Subway Connected Aisle: A Case Study

Yumin Yang,Zhongwei Cai,Nan Jiang,Chuanbo Zhou,Haibo Li 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.2

How to ensure the underground gas pipe’s safety under blasting construction of subway connected aisles is a key problem in tunnel construction. Based on the blasting excavation project of Wuhan Rail Transit Line 8 phase II, the numerical model whose reliability is verified by field monitoring data is established by ANSYS/LS-DYNA to analyze the dynamic underground gas pipe’s dynamic characteristics under blasting excavation of the connected aisle. According to the piping design code, the allowable effective stress control standard of underground pipe is proposed to evaluate the underground pipe’s safety under the blasting. The results show that: with the advance of blasting excavation, the position of the most dangerous section will change constantly and the pipe’s peak particle velocity (PPV) will appear an obvious amplification phenomenon in both sides of metro tunnels. The pipe’s PPV is greater than the ground surface’s PPV. A mathematical model is established to predict the pipeline’s PPV based on the linear relationship between the pipeline and the ground surface. According to the correlation between PPV and von-Mises stress of gas pipeline, a mathematical model between the pipe’s von-Mises stress and distance is established to predict the pipeline’s safety, which can provide guidance for actual blasting engineering.

The Development of Clinical Document Standards for Semantic Interoperability in China

Peng Yang,Feng Pan,Danhong Liu,Yongyong Xu,Yi Wan,Haibo Tu,Xuejun Tang,Jianping Hu 대한의료정보학회 2011 Healthcare Informatics Research Vol.17 No.4

Objectives: This study is aimed at developing a set of data groups (DGs) to be employed as reusable building blocks for the construction of the eight most common clinical documents used in China’s general hospitals in order to achieve their structural and semantic standardization. Methods: The Diagnostics knowledge framework, the related approaches taken from the Health Level Seven (HL7), the Integrating the Healthcare Enterprise (IHE), and the Healthcare Information Technology Standards Panel (HITSP) and 1,487 original clinical records were considered together to form the DG architecture and data sets. The internal structure, content, and semantics of each DG were then defined by mapping each DG data set to a corresponding Clinical Document Architecture data element and matching each DG data set to the metadata in the Chinese National Health Data Dictionary. By using the DGs as reusable building blocks, standardized structures and semantics regarding the clinical documents for semantic interoperability were able to be constructed. Results: Altogether, 5 header DGs, 48 section DGs, and 17 entry DGs were developed. Several issues regarding the DGs, including their internal structure, identifiers,data set names, definitions, length and format, data types, and value sets, were further defined. Standardized structures and semantics regarding the eight clinical documents were structured by the DGs. Conclusions: This approach of constructing clinical document standards using DGs is a feasible standard-driven solution useful in preparing documents possessing semantic interoperability among the disparate information systems in China. These standards need to be validated and refined through further study.

Hematopoietic Reconstitution of CD34+ Cells Derived from Short-term Cultured Cord Blood Mononuclear Cells

Shi Yang,Haibo Cai,Huili Jin,Jinli Fan,Wen-song Tan 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.4

Ex vivo expansion of hematopoietic stem cells (HSCs) is very important for clinical applications of cord blood (CB). With the aim to find proper culture duration for ex vivo expansion, mononuclear cells (MNC) was applied as starting culture cells to expand HSCs and the repopulating potential of seven-day and fourteen-day cultured CD34+ cells were compared. The average expansion of total cells and CD34+ cells cultured for 7 days were higher than those cultured for 14 days. The results of phenotypic analysis of fresh and cultured cells showed that the percentage of CD3+ cells declined and the percentage of CD33+ cells increased during culture. The engraftment levels of fourteen-day cultured CD34+ cells were higher than those of fresh and seven-day cultured CD34+ cells. Fourteen-day cultured CD34+ cells also showed better multilineage reconstitution ability than fresh and seven-day cultured CD34+ cells. The results of the present study demonstrated that prolonged culture could preserve the hematopoietic reconstitution ability of ex vivo cultured CB cells and improve the engraftment level in NOD/SCID mice.

Formation Mechanisms for (Cr,Co)7C3/(Cr,Co)23C6 Heterogeneous Precipitates and Stacking Faults Around Carbides in Surfacing Welding of Stellite Alloy on Stainless Steel

Jiankun Xiong,Haibo Zhang,Fuheng Nie,Fen He,Jianping Yang,Zhan Hu,Zhongbo Wen,Haiyan Zhao,Xinjian Yuan 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.7

The formation mechanisms for two carbides with co-existence and increasing stacking faults nearby the carbides duringsurfacing welding were investigated in this study. The results indicated that the surfacing layer had a two-phase structure ofa matrix phase and a second phase, the density of the second phase was relatively small and the second phase was discontinuous,by contrast with the as-received welding wire. Relatively uniform microstructure of surfacing layer and the weldinginterface without melting of base metal were obtained, resulting in an inapparent change in the microhardness. A few carbideswith blocky shape were identified in the surfacing layer. The mechanisms for (Cr,Co)23C6 of face-centered cubic crystalstructure co-existing with (Cr,Co)7C3 of hexagonal close-packed crystal structure and (Cr,Co)23C6 subsequently forming closeto the pre-formed (Cr,Co)7C3 during the cooling process were discussed in alloying element, calculated equilibrium phasediagram, Gibbs energy, etc. The relatively higher density of the stacking fault present around the carbides was understoodfrom forming energy of stacking faults.