Effects of Quenching Methods and Aging Processes on the Crushing Properties and Microstructure of Al–Zn–Mg Alloy Thin-Walled Square Extrusions

Hui Guo,Jin Zhang,Cheng Wang,Yun‑lai Deng 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.8

In this study, the effects of different (online quenching and offline quenching) quenching methods and aging processes (T6and T73) on the crashworthiness and microstructural evolution of three Al–Zn–Mg alloys (G1–G3) were studied by conductingtensile test and axial compression tests at room temperature, combined with optical metallography, electron back scattereddiffraction, and transmission electron microscopy microstructural observations. The obtained results revealed that thecrushing properties of three different Al–Zn–Mg alloys subjected to different quenching methods and aging processes weresignificantly different. Their crushing energy absorption of are ranked as follows: G1 > G3 > G2. The highest total energyabsorption gap (between T6 and T73) is the G1 alloy, and the lowest one is the G3 alloy. The largest total energy absorptiongap between the two quenching methods is the G3 alloy, and the smallest one is the G1 alloy. The G2 alloy with the largesttotal amount of Zn + Mg has the highest number density of matrix precipitates, the largest precipitate gap (between T6 andT73) and the smallest precipitate gap between the two quenching methods. The G3 alloy with the largest Zn/Mg ratio hasthe smallest number density of matrix precipitates, the minimum precipitation gap (between T6 and T73) and the maximumprecipitation gap between the two quenching methods. The G1 alloy with the lowest Zn/Mg ratio has the smallest size ofgrain boundary precipitates and PFZ width, while their largest values are obtained for the G3 alloy with the maximum Zn/Mg ratio. As a crushing resistant structural material, the crushing properties is improved without reducing the strength. Theratio of Zn/Mg should be controlled within the range of 4.57–6.15, while the total amount of Zn + Mg should be controlledwithin the range of 6.18–7.01.

Favorable Impact Toughness of High Heat Input Coarse-Grained HAZ in an Ultra-Low Carbon High-Strength Microalloyed Steel

Fuhui Cui,Junjie Wang1,Xiangdong Gan,Dawei Yu,Ilya Vladimirovich Okulov,Qinghua Tian,Xueyi Guo 대한금속ᆞ재료학회 2023 METALS AND MATERIALS International Vol.29 No.2

Liquid Mg is capable of quickly eroding the refractory Ni-based superalloy and selectively dissolve Ni, which opens up anew avenue for the recycling of superalloy scraps for comprehensive metal reclamation. This study aims at investigating theunderlining mechanism and kinetics of the selective dissolution of Ni from Inconel 718 (one type of Ni-based superalloy)with liquid Mg, by experimenting at 750, 800, and 850 °C for various amounts of time in an inert atmosphere. This selectivedissolution process is also known as liquid metal dealloying. Results show that a permeable dealloyed layer was formedresulting from the preferential dissolution of Ni over other metallic elements (e.g., Fe, Cr, Nb, Mo, and Ti). At the dealloyingforefront, the grain boundaries of the matrix γ phase were preferentially eroded by liquid Mg. The opposite concentrationgradients of Ni (4–9 wt%) and Mg (12–18 wt%) cross the dealloyed layer suggested their counter diffusion. A higher heatingtemperature substantially increased the dealloying rate.

Recommendations on management of gynecological malignancies during the COVID-19 pandemic: perspectives from Chinese gynecological oncologists

Yingmei Wang,Shiqian Zhang,Lihui Wei,Zhongqiu Lin,Xinyu Wang,Jianliu Wang,Keqin Hua,Manhua Cu,Jiandong Wang,Shixuan Wang,Wen Di,Yudong Wang,Ruifang An,Mingrong Xi,Ruixia Guo,Qi Zhou,Xing Xie,Fengxia X 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.4

The outbreak of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratorysyndrome coronavirus 2 has rapidly spread globally. Cancer patients are at a higher risk ofbeing infected with the coronavirus and are more likely to develop severe complications, ascompared to the general population. The increasing spread of COVID-19 presents challengesfor the clinical care of patients with gynecological malignancies. Concerted efforts should beput into managing gynecological malignancies in an orderly manner by strictly implementingthe measures that are specifically developed for controlling the spread of COVID-19. We havedrafted Recommendations on Management of Gynecological Malignancies during the COVID-19 Pandemicbased on our experience on controlling COVID-19 pandemic in China. We recommendthat patients with gynecological malignancies should be managed in hierarchical andindividualized manners in combination with local conditions related to COVID-19. Medicalcare decision should be balanced between controlling COVID-19 pandemic spread and timelydiagnosis and treatment for gynecologic oncology patients.

Effect of Dietary Antioxidant and Energy Density on Performance and Anti-oxidative Status of Transition Cows

Wang, Y.M.,Wang, J.H.,Wang, C.,Wang, J.K.,Chen, B.,Liu, J.X.,Cao, H.,Guo, F.C. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.10

This study was conducted to evaluate the effect of dietary antioxidant and energy density on performance and antioxidative status in transition cows. Forty cows were randomly allocated to 4 dietary treatments in a $2{\times}2$ factorial design. High or low energy density diets (1.43 or 1.28 Mcal $NE_L$/kg DM, respectively) were formulated with or without antioxidant (AOX, a dry granular blend of ethoxyquin and tertiary-butylhydroquinone; 0 or 5 g/cow per d). These diets were fed to cows for 21 days pre-partum. During the post-partum period, all cows were fed the same lactation diets, and AOX treatment followed as for the pre-partum period. Feeding a high energy diet depressed the DMI, milk yield, and 4% fat-corrected milk (FCM) of cows. However, AOX inclusion in the diet improved the milk and 4% FCM yields. There was an interaction of energy density by AOX on milk protein, milk fat and total solids contents. Feeding a high energy diet pre-partum increased plasma glucose and ${\beta}$-hydroxybutyrate, whereas dietary AOX decreased plasma ${\beta}$-hydroxybutyrate value during the transition period. There were also interactions between time and treatment for plasma glutathione peroxidase activity and malondialdehyde content during the study. Cows fed high energy diets pre-partum had higher plasma glutathione peroxidase activity 3 days prior to parturition, compared with those on low energy diets. Inclusion of AOX in diets decreased plasma glutathione peroxidase activity in cows 3 and 10 days pre-partum. Addition of AOX significantly decreased malondialdehyde values at calving. Energy density induced marginal changes in fatty acid composition in the erythrocyte membrane 3 days post-partum, while AOX only significantly increased cis-9, trans-11 conjugated linoleic acid composition. The increase in fluidity of the erythrocyte membrane was only observed in the high energy treatment. It is suggested that a diet containing high energy density pre-partum may negatively affect the anti-oxidative status, DMI and subsequent performance. Addition of AOX may improve the anti-oxidative status and reduce plasma ${\beta}$-hydroxybutyrate, eventually resulting in improved lactation performance; the response to AOX addition was more pronounced on the high energy diet.

Role of Dehydrocorybulbine in Neuropathic Pain After Spinal Cord Injury Mediated by P2X4 Receptor

Wang, Zhongwei,Mei, Wei,Wang, Qingde,Guo, Rundong,Liu, Peilin,Wang, Yuqiang,Zhang, Zijuan,Wang, Limin Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.2

Chronic neuropathic pain is one of the primary causes of disability subsequent to spinal cord injury. Patients experiencing neuropathic pain after spinal cord injury suffer from poor quality of life, so complementary therapy is seriously needed. Dehydrocorybulbine is an alkaloid extracted from Corydalis yanhusuo. It effectively alleviates neuropathic pain. In the present study, we explored the effect of dehydrocorybulbine on neuropathic pain after spinal cord injury and delineated its possible mechanism. Experiments were performed in rats to evaluate the contribution of dehydrocorybulbine to P2X4 signaling in the modulation of pain-related behaviors and the levels of pronociceptive interleukins and proteins after spinal cord injury. In a rat contusion injury model, we confirmed that chronic neuropathic pain is present on day 7 after spinal cord injury and P2X4R expression is exacerbated after spinal cord injury. We also found that administration of dehydrocorybulbine by tail vein injection relieved pain behaviors in rat contusion injury models without affecting motor functions. The elevation in the levels of pronociceptive interleukins ($IL-1{\beta}$, IL-18, MMP-9) after spinal cord injury was mitigated by dehydrocorybulbine. Dehydrocorybulbine significantly mitigated the upregulation of P2X4 receptor and reduced ATP-evoked intracellular $Ca^{2+}$ concentration. Both P2XR and dopamine receptor2 agonists antagonized dehydrocorybulbine's antinociceptive effects. In conclusion, we propose that dehydrocorybulbine produces antinociceptive effects in spinal cord injury models by inhibiting P2X4R.

Draft Genome Assembly and Annotation for Cutaneotrichosporon dermatis NICC30027, an Oleaginous Yeast Capable of Simultaneous Glucose and Xylose Assimilation

Wang Laiyou,Guo Shuxian,Zeng Bo,Wang Shanshan,Chen Yan,Cheng Shuang,Liu Bingbing,Wang Chunyan,Wang Yu,Meng Qingshan 한국균학회 2022 Mycobiology Vol.50 No.1

The identification of oleaginous yeast species capable of simultaneously utilizing xylose and glucose as substrates to generate value-added biological products is an area of key eco- nomic interest. We have previously demonstrated that the Cutaneotrichosporon dermatis NICC30027 yeast strain is capable of simultaneously assimilating both xylose and glucose, resulting in considerable lipid accumulation. However, as no high-quality genome sequenc- ing data or associated annotations for this strain are available at present, it remains chal- lenging to study the metabolic mechanisms underlying this phenotype. Herein, we report a 39,305,439 bp draft genome assembly for C. dermatis NICC30027 comprised of 37 scaffolds, with 60.15% GC content. Within this genome, we identified 524 tRNAs, 142 sRNAs, 53 miRNAs, 28 snRNAs, and eight rRNA clusters. Moreover, repeat sequences totaling 1,032,129 bp in length were identified (2.63% of the genome), as were 14,238 unigenes that were 1,789.35 bp in length on average (64.82% of the genome). The NCBI non-redundant protein sequences (NR) database was employed to successfully annotate 11,795 of these unigenes, while 3,621 and 11,902 were annotated with the Swiss-Prot and TrEMBL databases, respectively. Unigenes were additionally subjected to pathway enrichment analyses using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups of proteins (COG), Clusters of orthologous groups for eukaryotic com- plete genomes (KOG), and Non-supervised Orthologous Groups (eggNOG) databases. Together, these results provide a foundation for future studies aimed at clarifying the mech- anistic basis for the ability of C. dermatis NICC30027 to simultaneously utilize glucose and xylose to synthesize lipids.

A New Semi-supervised Classification Method of Hyperspectral Image based on Combining Renyi Entropy and Multinomial Logistic Regression Algorithm

Chunyang Wang,Shuangting Wang,Zengzhang Guo,Liping Wang,Chao Ma 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5

The study on classification methods of hyperspectral image is a focal growing area in remote sensing applications because the wide spectral range, providing a very high spectral resolution, allows the detection and classification surfaces and chemical elements of the observed image. Semi-supervised learning method which takes a large number of unlabeled samples and minority labeled samples, improving classification and predicting the accuracy effectively have been a new research direction. In this paper we proposed a new semi-supervised classification method of hyperspectral image based on combining Renyi entropy and multinomial logistic regression algorithm. The multinomial logistic regression was performed to describe a direct relationship between the selected sample as and their category. A lot of unlabeled samples are constantly added to the sample data using Renyi entropy algorithm. The test analysis of image classification in test area showed the advantages of classification method based on combining Renyi entropy and multinomial logistic regression algorithm for hyperspectral remote sensing image.

Cardiac-targeted delivery of nuclear receptor RORα via ultrasound targeted microbubble destruction optimizes the benefits of regular dose of melatonin on sepsis-induced cardiomyopathy

Shanjie Wang,Kegong Chen,Ye Wang,Zeng Wang,Zhaoying Li,JunChen Guo,Jianfeng Chen,Wenhua Liu,Xiaohui Guo,Guangcan Yan,Chenchen Liang,Huai Yu,Shaohong Fang,Bo Yu 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Large-dose melatonin treatment in animal experiments was hardly translated into humans, which may explain the dilemma that the protective effects against myocardial injury in animal have been challenged by clinical trials. Ultrasound-targeted microbubble destruction (UTMD) has been considered a promising drug and gene delivery system to the target tissue. We aim to investigate whether cardiac gene delivery of melatonin receptor mediated by UTMD technology optimizes the efficacy of clinically equivalent dose of melatonin in sepsis-induced cardiomyopathy. Methods Melatonin and cardiac melatonin receptors in patients and rat models with lipopolysaccharide (LPS)- or cecal ligation and puncture (CLP)-induced sepsis were assessed. Rats received UTMD-mediated cardiac delivery of RORα/cationic microbubbles (CMBs) at 1, 3 and 5 days before CLP surgery. Echocardiography, histopathology and oxylipin metabolomics were assessed at 16–20 h after inducing fatal sepsis. Results We observed that patients with sepsis have lower serum melatonin than healthy controls, which was observed in the blood and hearts of Sprague–Dawley rat models with LPS- or CLP-induced sepsis. Notably, a mild dose (2.5 mg/kg) of intravenous melatonin did not substantially improve septic cardiomyopathy. We found decreased nuclear receptors RORα, not melatonin receptors MT1/2, under lethal sepsis that may weaken the potential benefits of a mild dose of melatonin treatment. In vivo, repeated UTMD-mediated cardiac delivery of RORα/CMBs exhibited favorable biosafety, efficiency and specificity, significantly strengthening the effects of a safe dose of melatonin on heart dysfunction and myocardial injury in septic rats. The cardiac delivery of RORα by UTMD technology and melatonin treatment improved mitochondrial dysfunction and oxylipin profiles, although there was no significant influence on systemic inflammation. Conclusions These findings provide new insights to explain the suboptimal effect of melatonin use in clinic and potential solutions to overcome the challenges. UTMD technology may be a promisingly interdisciplinary pattern against sepsis-induced cardiomyopathy.

Role of Dehydrocorybulbine in Neuropathic Pain After Spinal Cord Injury Mediated by P2X4 Receptor

Zhongwei Wang,Wei Mei,Qingde Wang,Rundong Guo,Peilin Liu,Yuqiang Wang,Zijuan Zhang,Limin Wang 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.2

Chronic neuropathic pain is one of the primary causes of disability subsequent to spinal cord injury. Patients experiencing neuropathic pain after spinal cord injury suffer from poor quality of life, so complementary therapy is seriously needed. Dehydrocorybulbine is an alkaloid extracted from Corydalis yanhusuo. It effectively alleviates neuropathic pain. In the present study, we explored the effect of dehydrocorybulbine on neuropathic pain after spinal cord injury and delineated its possible mechanism. Experiments were performed in rats to evaluate the contribution of dehydrocorybulbine to P2X4 signaling in the modulation of pain-related behaviors and the levels of pronociceptive interleukins and proteins after spinal cord injury. In a rat contusion injury model, we confirmed that chronic neuropathic pain is present on day 7 after spinal cord injury and P2X4R expression is exacerbated after spinal cord injury. We also found that administration of dehydrocorybulbine by tail vein injection relieved pain behaviors in rat contusion injury models without affecting motor functions. The elevation in the levels of pronociceptive interleukins (IL-1β, IL-18, MMP-9) after spinal cord injury was mitigated by dehydrocorybulbine. Dehydrocorybulbine significantly mitigated the upregulation of P2X4 receptor and reduced ATP-evoked intracellular Ca2+ concentration. Both P2XR and dopamine receptor2 agonists antagonized dehydrocorybulbine’s antinociceptive effects. In conclusion, we propose that dehydrocorybulbine produces antinociceptive effects in spinal cord injury models by inhibiting P2X4R.

Proteasome subunit beta type 1 interacts directly with Rheb and regulates the cell cycle in Cashmere goat fetal fibroblasts

Yanfeng Wang,Xudong Guo,Zhigang Wang,Zhixin Guo,Mingtao Liu,Keyu Zhao,Xue Feng,Xu Zheng,Xiaojing Wang,Huifang Hao 한국통합생물학회 2017 Animal cells and systems Vol.21 No.5

PSMB1 (proteasome subunit beta type 1) is a core component of the 20S proteasome, and based on its structure, it might have a crucial function in the transcription of certain genes. Rheb (Ras homolog enriched in brain) is a Ras-like small GTPase that acts as an upstream positive regulator of the mechanistic target of rapamycin complex 1 (mTORC1) pathway. We cloned and characterized PSMB1 (KY310590.1) to determine its function in cell cycle progression and proliferation of goat fetal fibroblasts (GFbs). Further, by yeast two-hybrid screen and coimmunoprecipitation, we conformed that PSMB1 interacts directly with Rheb. An siRNA was designed and expressed targeting PSMB1 mRNA in GFbs and inducing cell cycle arrest. Rheb overexpression in GFbs significantly increased the number of S phase cells and growth efficiency compared with control cells. These data indicate that PSMB1 and Rheb have important functions in the cell cycle and proliferation of GFbs, indicating that their interaction governs many processes in GFbs.