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      • 난관배양액이 처녀발생유기된 돼지난포란의 체외발달에 미치는 영향

        문승주,이경호,김호,김창렬,은대숙,김광현,나진수,김재홍 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

        본 연구는 난관배양액이 돼지수정란의 체외발달에 미치는 효과를 규명키 위하여 수행하였다. 돼지 미성숙 난포란은 TCM-199, Ham's F-10 그리고 Whitten's 배양액에 10% 난포액과 호르몬(PMSG : 10IU/㎖, HCG : 10IU/㎖)을 첨가 20시간 배양하고 호르몬을 첨가하지 않는 배양액에서 20시간 추가 배양하여 총 40시간동안 배양하여 체외성숙을 유도하였다. 체외성숙후 0.1% hyaluronidase로 난구세포를 제거하고 15% FCS가 함유된 TCM-199으로 3회 세척하고 TCM-199에 15% FCS와 10% ethanol 혼합액에 세척한 난자를 옮겨 10분간 배양 처녀발생을 유기하였다. 처녀발생 6시간후 전핵형성율은 체외성숙배양액으로 TCM-199을 사용했을 때 56.4%, Ham's F-10의 경우 58.3%, Whitten's 배양액의 경우 74.0%를 보였다. 처녀발생 유기 48시간째 난할율은 TCM-199을 사용했을 때 45.7%, Ham's F-10에서 45.4%, Whitten's배양액에서 39.2%를 보였으며 세종류의 배양액에 POCM을 첨가 배양했을 때 TCM-199에 44.8%, Ham's F-10에서 45.4%, Whitten's배양액에서 43.7%로 나타났다. 처녀발생육 난자를 96시간 체외배양시킨 결과 상실배 발달율이 POCM을 첨가 했을 때 첨가하지 않은 시험구에 비하여 유의적으로 높았다(P<0.05) The effect of porcine oviductal conditioned medium(POCM) on in vitro development of chemically activated porcine oocytes was studied. Porcine oocytes were cultured in TCM-199, Ham's F-10 and Whitten's medium with hormonal supplements for 20h and 40h additional culture without hormonal supplements. After in vitro maturation, the denuded oocytes were washed 3 times with TCM-199 contaning 15%(v/v) ethanol to induce pathenogenetic activation. At 6h after activation, pronuclea formation rates were 56.4% in TCM-199, 59.3% in Ham's F-10 and 74.0% in Whitten's maturation medium. At 48h after activation, 45.7%, 45.4% and 39.2% of oocytes claved in TCM-199, Ham's F-10 and Whitten's culture medium, respectively. And 44.8%, 45.5% and 43.7% of oocytes were claved in TCM-199, Ham;s F-10 and Whitten's culture medium supplemented with POCM, respectively. The rates of moular were higher in culture medium with POCM than without POCM at 96h after activation.(P<0.05)

      • 난관액, 난관배양액 및 난포액이 돼지정자의 운동성과 수정능 획득에 미치는 영향

        문승주,이경호,김호,은대숙,김창렬,김광현,나진수,김재홍 全南大學校 農業科學技術硏究所 1997 農業科學技術硏究 Vol.32 No.-

        본 연구는 돼지 정자의 운동성과 첨체반응에 대한 난관액, 난관배양액 및 난포액의 효과를 조사하였다. 수정능획득배양액에 난관액(10%)을 첨가 공배양했을 때 정자의 운동성은 처리구에서 유의적으로 높았다(P<0.05). CTC 염색방법으로 정자의 첨체반응을 조사한 결과 난관액과 난포액이 첨가된 수정능획득 배양액에서 1.5, 3, 4.5시간동안 전배양을 실시하였을 때 정자의 첨체반응이 대조구에 비하여 유의적으로 높았으며(P<0.05), 난관배양액에서 1.5, 3, 4.5시간동안 전배양을 실시하였을때도 대조구에 비하여 정자첨체반응이 유의적으로 높았다(P<0.05). 이러한 연구결과는 난관과 난포액등에 함유되어있는 어떤인자가 정자의 운동성과 첨체반응율을 높힌다고 사료된다. The effects of oviductal fluid, oviductal conditioned medium and follicular fluid on porcine sperm mortility and capacitation were studied. The addition of oviductal fluid, oviductal conditioned medium and follicular fluid to the prefertilization medium increased sperm mortility(P<0.05). Chloroterracycline(CTC) flourescence patterns were used to determine incidence of acrosome reaction. The proportion of acrosome reaction increased when spermatozoa were exposed for 1.5, 3 and 4.5h to oviductal fluid and follicular fluid(P<0.05). In sperm-POCM(porcine oviductal conditioned medium) co-culture period of 4.5 h, acrosome reaction rates was higher than other groups(P<0.05). These results suggest that the factor(s) in secreation from oviduct and follicular fluid increased sperm mortility and acrosome reaction.

      • IMx Glycated Hemoglobin측정법에 관한 연구

        황성준,박종성,이승관,이창규,류정록,변대훈,박은병 高麗大學校 倂設 保健大學 保健科學硏究所 1994 保健科學論集 Vol.20 No.1

        Percent glycated hemoglobin(%GHb) is an indirect measure of the mean blood glucose level in an individual over the previous two to three months. A fully automated assay for quantitating %GHb in human whole blood has been developed using the Abbot IMx R analyzer. The assay is accomplished by specific solution phase tagging of glycated hemoglobin by a soluble polyanionic affinity reagent, and subsequent capture of the anionic complex by a cationic solid phase matrix. Both glycated hemoglobin(GHb) and total hemoglobin(Hb) are quantitated by measuring quenching of the fluorescence from an added dye by home. A correlation study was conducted accross IMx R instruments with fully automated HPLC (Hi-Auto A_(IC TM), Kyoto Daiiclii co.) as the reference assay. The correlation statistics obtained for 20 diabetic specimens were: IMx=0.84 HPLC+1.75, correlation coefficient= 0.969. Studies from linear relationship statistics had showed that there ere no evidence for difference between two methods. IMx glycated hemoglobin assay was don-sidered to be compatible with HPLC method.

      • SCIEKCI등재SCOPUS

        Moutan radix 의 mouse sarcoma 180 cell 로 유발한 mouse ascites cancer 에 대한 항암효과

        반경녀(Kyeong N . Bahn),이은주(Eun J . Lee),양민석(Min S . Yang),김정옥(Jeong O . Kim),하영래(Yeong L . Ha) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.4

        Anticarcinogenic activity of Moutan radix for mouse ascites cancer induced by mouse Sarcoma 180 (S-180) cells was investigated. Methanol extract of Moutan radix including other folk medicinal plants (Taxus cuspidata, Curcuma Tonga, Artemisia capillaris, Ligrstri fructus, and Liriope platyphylla) used to remedy or cure many chronic human diseases like cancer was fractionated into hexane, chloroform (CHCl₃), ethylacetate (EtOAc), and butanol (BuOH) fractions. Anticarcinogenic activity of the fractions, exhibited a strong cytotoxicity for L1210 and S-180 cells, was examined for mouse ascites cancer induced by S-180 cells. Male ICR mice (7 mice/treatment, 5∼6 weeks of age, 23±1 g) were injected i.p. with S-180 cells (1×10^7 cell/1 ㎖ PBS). One day later, each mouse was given 0.1 ㎖ of 10% DMSO containing sample (30 ㎍/g body weight) every day for 10 consecutive days. Control mice were only given 0.1 ㎖ S-180 cells and 0.1 ㎖ 10% DMSO. Mice treated with EtOAc fraction of Moutan radix showed 28.7 days of life, which is 167% of control mice`s life. Based on the dose-dependant experiment, mice treated with 30 ㎍ showed longer life relative to mice treated with other doses (5, 15, 60 ㎍) and mice treated with 60 ㎍ exhibited toxic symptoms. Body weight of mice treated with Moutan radix was significantly reduced relative to that of control mice (p$lt;0.05). GC-MS analysis in conjunction with silica-gel column chromatography revealed that the EtOAc fraction contained 2-methoxylphenol, benzoic acid, 1-(4-hydroxy-3-methoxyphenyl)ethanone, 8-methyl-2,4(1H,3H)pteridinedione and 2,5-furan-dicarboxylic dimethyl ester as regards to the anticarcinogenic property of the EtOAc fraction. These results suggest that Moutan radix might be included as an anticarcinogenic medicinal plant for treatment of ascites cancer.

      • KCI등재

        Cloning and induction patterns of Cu/Zn superoxide dismutase in responses to immune elicitors and nucleopolyhedrovirus in the beet armyworm Spodoptera exigua

        Mi Y. NOH,조용훈,Cheol H. PARK,Eun S. LEE,Hyo J. LEE,김익수,이용석,Sook J. SEO,In S. BANG,김선곤,한연수 한국곤충학회 2010 Entomological Research Vol.40 No.6

        Superoxide dismutase (SOD) is a metalloenzyme that catalyzes the dismutation of the superoxide anion to oxygen and hydrogen peroxide, and has accordingly been known to play an important role in reducing oxidative stresses induced by various stresses and infection. In an attempt to investigate whether it is involved in antiviral immune responses in insects, the full-length cDNA of SeCu/ZnSOD was obtained from the cDNA library of Spodoptera exigua. It is 1,008 bp long (excluding poly-A tail) with the open reading frame of 459 bp encoding 152 amino acids. Sequence analysis with BLAST shows identities to various insects such as Bombyx mori (80%), Hyphantria cunea (80%), Plutella xylostella (77%), Aedes aegypti (73%), Apis mellifera (71%), Lasius niger (70%), Drosophila melanogaster (69%) and Gryllotalpa orientalis (67%). During development, S. exigua expressed SeCu/ZnSOD predominantly at embryonic stage, pupal stage and adult female stage, whereas it was slightly expressed in larvae and adult male. In addition, SeCu/ZnSOD was highly expressed in the fat body, hemocyte, silk gland and Malpighian tubule, although the levels of SeCu/ZnSOD transcripts were very low in the gut. Furthermore, SeCu/ZnSOD mRNA was slightly induced in response to either lipopolysaccharide or laminarin. Finally, SeCu/ZnSOD mRNA was induced in carcass in response to S. exigua nucleopolyhedrovirus (SeNPV) infection, whereas it was not strikingly induced in the gut. The precise roles of SeCu/ZnSOD in antiviral immune response remains to be further studied.

      • KCI등재후보

        Protective Effects of N-acetylcystenine and Selenium against Doxorubicin Toxicity in Rats

        Yeo-sung Yoon,Sun-don Kim,Min-hye Lee,Heung-shik S.Lee,In-se Lee,Je-kyung Sung,Eun-sung Park 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

        Effects of N-acetylcysteine and Selenium against Doxorubicin Toxicity in RatsEun-sung Park, Sun-don Kim1, Min-hye Lee, Heung-shik S. Lee, In-se Lee, Je-kyung Sung and Yeo-sung Yoon*

      • Conserved sequences of thrombospondin‐related adhesive protein gene of <i>Plasmodium vivax</i> in clinical isolates from Korea

        Nam, Myung H.,Jang, Jin W.,Kim, Hanna,Han, Eun T.,Lee, Won J.,An, Seong S. A.,Park, Ae S. D.,Lim, Chae S. Blackwell Publishing Ltd 2011 Tropical medicine & international health Vol.16 No.8

        <P><B>Summary</B></P><P>Thrombospondin‐related adhesive protein (TRAP) from <I>Plasmodium vivax</I> (<I>P.?vivax</I>) became one of the important vaccine candidates for malaria, because <I>P.?vivax</I> TRAP (PvTRAP) is responsible for the sporozoite–host interactions. PvTRAP polymorphisms in the isolates from Republic of Korea (ROK) were analysed, setting the valuable baseline data for the future vaccine developments and clinical trials with PvTRAP, as a strong vaccine candidate. A total of 54 isolates were collected in 2010. PvTRAP genes from above isolates were amplified and sequenced, and the results were analysed and compared against Sal‐1 strain. Sequencing analysis of 1424‐bp‐size PvTRAP PCR products revealed one major allelic type with six non‐synonymous substitutions, where S81T, E95D, I121V and T127R substitutions were found in region II, and K371N and A425E substitutions from region IV. The ROK isolates revealed the limited sequence polymorphisms in PvTRAP in comparison with the reported isolates from other nations.</P>

      • Hereditary nonpolyposis colorectal cancer in endometrial cancer patients

        Yoon, Sang Nam,Ku, Ja-Lok,Shin, Young-Kyoung,Kim, Kyung-Hee,Choi, Jin-Sung,Jang, Eun-Ja,Park, Hyoung-Chul,Kim, Duck-Woo,Kim, Min A.,Kim, Woo Ho,Lee, Taek Sang,Kim, Jae Weon,Park, Noh-Hyun,Song, Yong-S Wiley Subscription Services, Inc., A Wiley Company 2008 International journal of cancer: Journal internati Vol.122 No.5

        <P>Endometrial cancer is the second most common cancer in hereditary nonpolyposis colorectal cancer (HNPCC). It has often been overlooked to explore the possibility of HNPCC in endometrial cancer patients. Our study was to investigate how many HNPCC patients existed among endometrial cancer patients. Among patients who underwent hysterectomy for endometrial cancer at Seoul National University Hospital from 1996 to 2004, 113 patients were included, whose family history and clinical data could be obtained and tumor specimens were available for microsatellite instability (MSI) testing and immunohistochemical (IHC) staining of MLH1, MSH2 and MSH6 proteins. There were 4 (3.5%) clinical HNPCC patients fulfilling the Amsterdam criteria II, and 2 (2/4, 50%) of them carried MSH2 germline mutations. There were also 8 (7.1%) suspected HNPCC (s-HNPCC) patients fulfilling the revised criteria for s-HNPCC, and one (1/8, 12.5%) of them revealed MLH1 germline mutation. In 101 patients, who were not clinical HNPCC or s-HNPCC, 11 patients showed both MSI-high and loss of expression of MLH1, MSH2 or MSH6 proteins, and 2 (2/11, 18.2%) of them showed MSH6 germline mutations. In 113 patients with endometrial cancer, we could find 5 (4.4%) HNPCC patients with MMR germline mutation and 2 (1.8%) clinical HNPCC patients without identified MMR gene mutation. Family history was critical in detecting 3 HNPCC patients with MMR germline mutation, and MSI testing with IHC staining for MLH1, MSH2 and MSH6 proteins was needed in the diagnosis of 2 HNPCC patients who were not clinical HNPCC or s-HNPCC, especially for MSH6 germline mutation. © 2007 Wiley-Liss, Inc.</P>

      • Nanostructured glycan architecture is important in the inhibition of influenza A virus infection

        Kwon, Seok-Joon,Na, Dong Hee,Kwak, Jong Hwan,Douaisi, Marc,Zhang, Fuming,Park, Eun Ji,Park, Jong-Hwan,Youn, Hana,Song, Chang-Seon,Kane, Ravi S.,Dordick, Jonathan S.,Lee, Kyung Bok,Linhardt, Robert J. Nature Publishing Group, a division of Macmillan P 2017 Nature nanotechnology Vol.12 No.1

        <P>Rapid change(1) and zoonotic transmission to humans(2) have enhanced the virulence of the influenza A virus (IAV)(3). Neutralizing antibodies fail to provide lasting protection from seasonal epidemics(1),(4). Furthermore, the effectiveness of anti influenza neuraminidase inhibitors has declined because of drug resistances. Drugs that can block viral attachment and cell entry independent of antigenic evolution or drug resistance might address these problems. We show that multivalent 6'-sialyllactose-polyamidoamine (6SL-PAMAM) conjugates, when designed to have well-defined ligand valencies and spacings, can effectively inhibit IAV infection. Generation 4 (G4) 6SL-PAMAM conjugates with a spacing of around 3 nm between 6SL ligands (S3-G4) showed the strongest binding to a hemagglutinin trimer (dissociation constant of 1.6 x 10(-7) M) and afforded the best inhibition of H1N1 infection. S3-G4 conjugates were resistant to hydrolysis by H1N1 neuraminidase. These conjugates protected 75% of mice from a lethal challenge with H1N1 and prevented weight loss in infected animals. The structure-based design of multivalent nanomaterials, involving modulation of nanoscale backbone structures and number and spacing between ligands, resulted in optimal inhibition of IAV infection. This approach may be broadly applicable for designing effective and enduring therapeutic protection against human or avian influenza viruses.</P>

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