Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

기초주의 관점에서 본 인격교육

박의수 안암교육학회 2010 한국교육학연구 Vol.16 No.3

기초주의는 한국 교육철학의 정립을 위하여 한기언이 제창하고 발전시킨 “전통과 개혁의 조화를 통한 인간형성의 논리”이다. 이 논문은 기초주의의 개념과 구조를 명료화 하고, 기초주의의 전체 구조 속에서 ‘인격’이 차지하는 위상과 교육적 의미를 밝히고, 아울러 기초주의의 틀과 관점에서 인격교육의 과제와 방향을 모색하는데 목적이 있다. 이를 위하여 먼저 기초주의의 개념과 구조를 명료화하고 다음으로 기초주의 구조 속에서의 인격의 위상을 고찰한 후에 기초주의의 관점에서 한국교육의 본질적 문제를 진단하고 기초로서의 인격교육의 방향을 탐구하여 다음 몇 가지 결론을 얻었다. 첫째, 복잡한 기초주의 이념체계를 하나의 표로 정리함으로써 일목요연하게 기초주의의 개념과 구조를 이해할 수 있게 되었다. 둘째, 인격은 사람됨의 기초인 동시에 교육의 기초이며 최종 목표이다. 셋째, 오늘날 한국교육의 본질적 문제는 결국 총체적인 기초의 부실, 즉 기초학력의 부실과 인격적 기초의 부실에서 비롯되는 것이며, 따라서 그 해법은 지적․인격적 측면에서 기초 교육을 공고히 하는데서 찾아야 한다는 것이다. Foundationism(Kichojuii) is widely known as "a human shaping logic through a harmony of tradition and reformation" that Hahn, Ki-Un initiated and developed in order to establish Korean Educational Philosophy. The purpose of this study is to clarify the concept and structure of Foundationism and to disclose the position and educational meaning of character in the context of Foundationism and to research the tasks and directions of character education from the viewpoint of Foundationism. For these purposes, clarifying the meaning and structure of Foundationism was proceeded first and investigating the position and meaning of character was carried out and examining the essential problems of Korea education from the viewpoint of Foundationism and searching the direction of character education as a foundation was finally proceeded. The conclusions of this study are as followings: First, a table that can clearly understand the whole complex system of Foundationism was completed. Second, character is the base of human being, as well as education, and also the eternal goal of education. Third, the essential problems of contemporary Korea education were derived from the weakness of basic scholastic ability and sincerity. So the sure method of solving the problems is to strengthen the foundation of knowledge education, as well as character education.

高等學校 生物의 遺傳工學 槪念 導入을 위한 實驗 Model

박상익,윤의수,김종균 공주대학교 사범대학 과학교육연구소 1995 과학교육연구 Vol.26 No.1

제 4차 교육과정 이후 제 5차 교육과정으로 개편되면서 생물 교과서에 유전 공학 개념 및 응용면이 더욱 강조 되었고, 제 6차 교육과정에서는 더욱 심화된 내용이 소개될 것으로 본다. 특히 일본의 고교 생물에서는 유저너 공학에 관한 내용을 학생들이 실험 할 수 있게 수록되어 있다. 그리고 앞으로의 자연과학은 유전공학 분야에서도 눈부신 발전을 가져올 것으로 기대되면 이에 맞추어 고등학교에서 유전공학 개념의 이해를 위한 유전자의 삽입, 형질 전환, 조직 배양 등의 실험을 실시하는 것은 학생들에게 많은 흥미와 관심을 갖게 하며 탐구력 신장에도 도움을 줄 것으로 생각한다. 본 연구는 제 5차 교육과정에 따른 고등학교 생물 교과서에 소개된 유전공학 내용을 분석한 결과 및 잎 절편체가 모상근으로 형질전환되는 기초 실험의 결과를 토대로 다음과 같은 결론을 내리고 탐구적 생물교육 자료로 삼고자 한다. 1.Agrobacterium tumefacieus, Agrobacterium rhizogenes을 이용한 모상근 발생에 관한 기초 실험의 특징은 형질전환체를 가시적인 뿌리로 얻을 수 있어 관찰이 용이하다. 2.형질 전환이 되었음을 GUS 활성 검사에 의해 쉽게 증명할 수 있어 유전공학 개념 도입을 위한 실험으로 고등학교에 도입하는 데 어려움이 없을 것으로 본다. 3.실험에 이용한 균은 감염 부위에 모상근을 형성하는 이외는 다른 병증을 나타내지 않기 때문에 대외적인 문제 없이 학교의 실험실에서도 취급할 수 있다. 4.유전 공학 실험에 필요로하는 화합물은 일선 학교에 대부분이 준비되어 있으며 kanamycin, Claforan은 미량 필요한데 쉽게 적은 비용으로 구입할 수 있다. 5.실험 기구는 간이 배양실만 고안 사용하면, 고등학교에 준비되어 있는 기구를 이용하여 해결이 가능하다. 유전 공학에 관한 실험을 하는데 실험실의 환경 조건이나 다인수 학급에서 어려움이 있더라도, 학생들에게 실험을 통해 유전 공학 개념을 형성시키는 기회를 갖게하는 것은 매우 중요하다. 이렇게 하여 자연과학에 대한 흥미와 관심을 높여가는 것은 우리나라의 기초과학 발전에 도움이 될 것으로 본다. The concept of biotechnology and its practical application is much more emphasized in the fifth curriculum than in the fourths and further intensified contents are supposed to be introduced in the sixth. Especially highschool biology in Japan, the contents on the biotechnology are recorded for the students to experiment and the future natural science is expected to develop brilliantly in a field of biotechnology. Therefore, students' experiments on gene insertion, transformation, tissue culture and so on, for their understanding of biotechnology concepts, are thought to stimulate their curiosity and extend their spirit of inquiry. This study is meant as an teaching aid, and reaches the following conclusions on the basis of the results of the biotechnology contents introduced in the fifth highschool biology texts and those of basic experiments on the transformation from explant to hairy root. The characteristic of basic experiments on hairy root growth used by Agrobacteriurm tumefacieus, Agrobacterium rhizogenes is that the observation is easy, since the transformational object is a visible root. The experiments are likely to be introduced without difficulty for highschool biotechnology concepts, since the transformation is easily proved by the GUS activation test. The germs used in experiments don't cause other diseases except forming hairy roots in infected parts, so they can be treated in school laboratories without outside interferance. Almost all schools are equipped with the complex materials needed for biotechnology experiments and kanamycin and claforan, just a little quantity of which is needed, can be purchased at a low price. The instruments can be replaced with the preequipped ones in school, only the temporary growthchamber needs to be devised. Though there are difficulties in biotechnology experiments because of unsufficient condition in laboratories or classes made up of a large number of students, it is very important for the students to have opportunities to form biotechnology concepts through experiments. To promote curiosity and interest in natural science in this way can be helpful in national basic science development.

축력과 모멘트를 받는 가셋트 K형 상관접합부의 강도 추정에 관한 연구

박순정,신의균,김우범,정수영 忠南大學校 産業技術硏究所 1997 산업기술연구논문집 Vol.12 No.2

A numerical analysis and finite element analysis were performed to suggest formula of gusset-tube connection that is subjected to axial and lateral load. Through the finite element analysis, the infulence of parameters on the ultimate strength was estimated and design formula was obtained from the case-study of result of the finite element analysis. The formulas suggested by other authors and the analytical of this paper were compared. The primary parameter of the analysis are axial load, lateral load, thickness of the primary menber, diameter of the primary menber and length of the gusset-plate. It was noted that the strength of connetion by the previous proposed formulas was much differ from result of the finite element analysis, but the difference of result between the proposed formula and the finite element analysis was within thirty percentage range. On the future research, small scale and full scale test will be performed to verify a formula suggested by this paper.

지조의 현대 교육적 의미

박의수 교육철학회 2002 교육사 교육철학 Vol.27 No.-

The purpose of this study is to clarify the meaning of Jijo(consistency) in modern education that is the core spiritual value of Sunbi. Sunbi, as an intellectual, is an ideal man of Korea from ancient to present. And to explore how to apply it to modern education is the another purpose. And the methodology of this study is the historical and analytical approach. The results can be summarized as follow. First, The concept of Jijo can be defined as constant attitude of life of a person. Although it has been changed a little according to the changing life style of Sunbi, consistency of his principles, beliefs was not changed. Second, Jijo is directly concerned with intellectulality as a knowledge condition, honesty as a moral condition and courage as a volitional and emotional condition. The three conditions are also contents of Jijo. Intellectuality is a kind of a mariner's compass that shows the right direction of true life, honesty is the practical principle of Jijo, courage is a kind of a shield that defends it from various stresses and temptations. Third, cooperating knowledge education with character education will contribute not only the education of 'becoming man' but also cultivating creativities, because Jijo vasically seeks after harmony and unity on the basis of honesty and sincerity. Finally, Jijo in modern society means consistency of life principle and keeping a promise with friends, neighbors, etc. in the ordinary civic life, though Jijo in traditional society needed deep pains or self-sacrifices sometimes. So I believe that Jijo should be a universal value in present and future.

PbSnTe 單結晶 成長

朴東秀,李義完 慶北大學校 文理科大學 1978 文理學叢 Vol.5 No.-

The method of refining Pb and Sn, and the growth of PbSnTe single crystal were described. Approximately better than 6N of pure Pb and Sn were obtained by zone refining of 20 passes as 10cm/hr. Fairly large single crystals of Pb_1-xSn_xTe of stoichiometric were grown by a Bridgman method. Zone refining pure Pb and Sn, and commercial pure Te were used in this experiment. The growing rate in 12mm i.d. quartz ampoule was about 10mm/day. The crustal formation was investigated by X-ray Spectrometer and Metal Microscope. The growing facets were(100) plane.

忠州市 地域特性을 反暎한 共同住宅 計劃硏究 : 충주지역 거주자의 APT 선호특성을 중심으로

朴碩鉉,朴義權,柳顯紀,尹勝照,孫泰鎭,金基洙 충주대 2003 産業科學論文集 Vol.11 No.-

The purpose of this research is to analyze the correlation of the characteristics of dwellers and residential preference in apartment housing through the case study of 340 samples in 25 housing estates which were built in Chung-ju city The contents of this research consist of two main parts. The first part is to identify characteristics of dwellers, which might have an influence on residential preference in apartment housing. The second part is to identify the main physical elements of residential preference that can be influenced by the personal characteristics. Thus, this research will contribute to the establishment of the planning methods which increase residential preference in apartment housing, while promoting the quality of residential environment in Chung-ju city.

Fordyce Granules

박의수,송준영,최윤애 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.3

Fordyce granules are small yellow spots that are multifocal and slightly elevated. They may occur in clusters or coalesce to from yellow plaques. They are bilaterally symmetrical and are located most frequently on the buccal mucosa of the cheeks, the inner surface of the lips, and on the mucosa distal to the mandibular molars. Fordyce spots are extremely common: probably 80% of the population have them. They are often not noticeable in children until after puberty, and they seem to be more obvious in males, in patients with greasy skin and in the elderly. The granules are totally benign, though the occasional patient or physician becomes concerned about them or misdiagnoses them as thrush, lichen planus or other diseases. We present two cases of Fordyce granules a 64 and a 50 year-old male who have cancer phobia, showing multiple involvement of minute yellowish white colored spots on the buccal mucosa and inner surface of the lip. Histopathologic finding shows a group of small but mature sebaceous lobules situated around a small sebaceous duct leading to the surface epithelium. We did not give any specific treatment but gave reassurance, presently under observation.

金屬表面의 吸着特性에 關한 硏究 : 表面摩擦問題 Friction of Metals

朴東秀,李義完,朴準鐸 慶北大學校 1976 論文集 Vol.21 No.-

The coefficients of friction, μ of various metals(Au, Cu, Fe, Zn, brass, stainless-steel), including transition metals(Nb, Mo) and semiconductors(Si, InSb) were observed in air and in a vacuum, concerning with the surface adsorption and the physical properties of the materials. In this experiment most measurements were carried out for the surface covered with adsorbed oxide films and some for the poor clean surfaces. All the metal specimen were polycrystalline. Samples were prepared by means of dry-tool cut, mechanical polishing and smoothing, etching and cleaning. The relatively clean surfaces were obtained by heating in a vacuum of about 10^-5 torr. It was found that the presence of the adsorbed or combined gas films on the surface gave a reduction to the experimental values of μ compared with the values for clean surfaces of the same hardness. The values of μ were ranged from 0.4 to 0.9 for the contaminated surfaces, while 0.4 to nearly 1.2 for the clean surfaces. The roughness of contact surface and the load affected the values of μ for all materials, and μ was roughly proportional to the load. μ increased as the temperature of samples raised from the room tempertaure to about 350℃ for the relatively low melting point, while no change was observed for the samples of high melting point: namely, for those metals whose relaxation point lie slightly above room temperature, the coefficient of friction rose rapidly with rising temperature for those metals whose relaxation point lie above 350℃, μ remained independent of temperature. When specimens of two different metals are brought into contact, the softer surface must be deformed plastically by the harder surface. μ was independent of the hardness for the identical sample pairs. In the present study, the sliding process involved a junction-growth and a ploughing action, consequently the shearing and deformation. These two processes interact and it is shown that, if junction-growth is appreciable, tensile stress may develop in the trailing portion of the contact region.

한국교육사에 있어서 書院의 기원에 관한 재고찰

박의수 고려대학교 교육문제연구소 1998 敎育問題硏究 Vol.10 No.-