각종 난치성 혈액 질환에서의 비혈연간 골수이식

김동욱,한훈,김정아,김희제,민창기,엄현석,최정현,이종욱,한치화,홍영선,최일봉,신완식,민우성,김학기,김춘추,김원일,김동집 대한조혈모세포이식학회 1997 대한조혈모세포이식학회지 Vol.2 No.1

목적: 비혈연간 골수이식은 혈연내에 적절한 골수공여자가 없는 만성골수성백혈병, 고위험군의 급성별혁병, 면역억제치료에 실패한 재생불량성빈혈 및 각종 난치성 조혈모세포질환의 완치를 위한 표준적인 치료방법으로 정착되고 있다. 혈연간 표준적인 동종 이식에 비하여 비혈연간 이식시에는 생착부전, 이식편대숙주반응과 감염이 더 빈번하게 발생하며, 국내에서는 아직까지 체계적인 임상연구결과가 보고된 바 없었다. 이에 본 센터에서는 1995년 10월 이후로 약 20개월간 26예의 비혈연간 골수이식을 시행하였으며 3개월 이상의 추적관찰이 가능하여 이식초기 합병증의 관찰 및 분석이 가능하였던 20예의 환자를 대상으로 이식성적 및 문제점을 보고함으로써 새롭게 확대되고 있는 이 분야의 임상연구 및 진료의 활성화를 꾀하고자 한다. 방법: 각종 혈액 종양질환으로 비혈연간 이식을 시행한 총 26예의 환자중 3개월이상의 추적관찰이 가능하였던 20예를 대상으로 후향적으로 임상경과를 분석한 후 생존 분석을 시행하였고, 환자의 연령, 성별, 질병의 상태, 조식 적합 항원의 일치정도, 이식편대 숙주 반응의 유무와 생존기간과의 상관관계를 살펴보았다. 또한 표준위험군과 고위험군으로 나누어 생존율을 비교하였고 이식과 관계된 생착 부전, 이식편대숙주반응, 감염의 발생과 양상 그리고 그 합병증을 관찰하였다. 결과: 1. 환자와 공여자간에 HLA 불일치가 20예 중 4예에서 있었으며, 생착여부의 확인이 가능했던 17예 중 16예에서 생착이 확인되어 94.1%의 생착율을 보였다. 2. 급성이식편대숙주반응은 62.5%(10/16예)에서 발생하였으며 111도 이상의 급성의 이식편대숙주 반응은 25%(4/16예)에서 발생하였다. 만성이식편대숙주반응은 40%(2/5예)의 환자에서 발생하였으며 이들 모두 국소형으로 중증의 진행형 만성이식편대숙주반응이 관찰된 환자는 없었다. 3. 호흡기 합병증은 10예(50%)에서 발생하였으며 감염성 폐렴을 포함한 호흡기 합병증이 가장 흔한 일차적인 사망 원인이었다. 호흡기 합병증이 발생했던 10예중 6예가 감염에 의한 폐렴이 의심되었고 나머지 4예는 특발성 간질성 폐렴이었다. 4. 8.5개월의 중앙추적기간 중 35%의 생존율을 관찰할 수 있었고, 생존기간은0.5개월에서 15개월 (중앙치:4개월)이었다. 한편 고위험군은 25%(3/12예), 표준위험군은 50%(4/8)의 생존율을 관찰할 수 있었다. 5. 가장 흔한 사망 원인은 감염성 폐렴을 포함한 호흡기 합병증(6예)이었고, 이외의 사망 원인으로는 급성 이식편대숙주반응과 다장기부전이 각각 2예, 생착 부전, 간정맥 폐쇄, 그리고 재발이 각각 1예였다. Unrelated bone marrow transplantation(UBMT) has been increasingly recognized as the standard treatment for cure of chronic myelogenous leukemia, high risk acute leukemia, aplastic failed on immunotherapy, and the variety of refractory hematologic diseases in patients lacking a related donor. However, as compared to HLA identical sibing transplantation, UBMT carries higher incidence of graft failure, graft versus host disease(GVHD), and infection. In our center, 26 patients underwent UMBT between October 1995 and June 1997. The minimum follow-up of 3 months was possible in 20 patients, for whom early complications and clinical outcomes were assessed. The median age of the 20 patients was 24 years. 8 patients had standard risk disease and 12 patients had high risk disease. All patients received various preparative regimens including total body irradiation according to disease and disease status. 19 patients received CsA + short course MTX for GVHD prophylaxis. One patient received marrow that was depleted of T cells ex vivo using avidinbiotin column. The class I loci were typed by serological methods and HLA-A, HLA-B and HLA-DRB1. 3 additional pairs were one minor mismatched at the HLA-B locus. Another one patients was one major mismatched at the DRBI alleles. 17 patients were evaluable for engraftment. Successful enfraftment was confirmed in 16 patients(94.1%). Only one patient who was performed one major DRBI mismatched transplants experienced graft rejection. 16 patients were evaluable for acute GVHD. The overall incidence of acute GVHD developed in 4 patients(25%). Five patients were evaluable for the development of Ⅳ acute GVHD developed in 4 patients (25%). Five patients were evaluanle for the development of chronic GVHD. 2 patients(40%) developed limited chronic GVHD. Respiratory complications including pulmonary infection developed in 10 patients(50%) and these complications were the most common primary cause of death. Of these 10patients, 6 had pneumonia due to fungus(4 patients), pacterial (1 patient), and CMV infection (1 patient) and 4(20%) had idiopathic interstitial pneumonitis and/or adult respiratory distress syndrome. The duration of median follow- up was 8.5 months and 7 of 20 patients(35%) are alive at the time of this analysis with survival duration of 0.5 to 15 months(median survival duration: 4 months). The overall survival was 25% (3/12 patients) in high risk group and 50%(4/8 patients) in standard group. From these results, we can predict that the incidence and severity of GVHD in Korea are lesser than multiracial countries and the long-term survival of patients with standard risk disease can approach that of HLA matched sibling transplants. For the past two years, the performance of UBMT has been rapidly increasing and it will be possible to analyze much larger number of patients soon in Korea. In the future the problems of graft failure, GVHD, and infection due to long lasting immunocompromised status will need to be overcome by continued medical research. In addition, the volunteer donor pool will have to be expanded by the promotion of the national awareness of its need.

쐐기형 레일클램프에서 조의 회전을 방지하기 위한 적정 쐐기각에 대한 연구

한동섭,한근조,심재준,이성욱 東亞大學校 大學院 2005 大學院論文集 Vol.30 No.-

Recently many countries have concentrated their effort on the port automation, in order to be the Hub-port, that the ULCS (Ultra Large Container Ship) could come alongside the quay in their region. As the magnitude of the container ship increases, that of the container crane (used in quay) increases from 50ton-class to 61ton-class more and more. The wind velocity criteria to design the structures used in the port was upgraded from 30m/s to 40m/s due to change of the weather condition. When we design wedge type rail clamp which can protect container crane from sudden wind blast with constant clamping force regardless of the operating period, it is important to determine the angle of wedge within proper range. In this study we designed the wedge angle of the wedge-type rail clamp using the FEA.

수리계획기법을 이용한 효율적 물배분 시스템모델 : 서남부지역을 중심으로

한동욱,서종석 전남대학교 농업과학기술연구소 2000 農業科學技術硏究 Vol.35 No.-

In this study, mathematical programming was applied to solve for optimal water allocation in the south-western part of Korea, where water supply and demand are unbalanced. The water allocation system was developed to meet water balance in the region through water transfer between the Keum and the Young-San Rivers. The result of the study shows that water transfer may satisfy various water demands in the region until the year 2011 without building reservoirs.

한국 재래산양에서 Bovine Viral Diarrhea, Bovine Herpesvirus-1, Parainfluenza Type-3 바이러스에 대한 혈청학적 연구

한동운,권용국,문진산,윤소라,위성환,장환,탁동섭,이태욱,강문일 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.3

The 948 goat sera were serologically tested for ten pathogens including bovine viral diarrhea (BVD), bovine herpes virus type-1 (BHV-1) and parainfluenza virus type-3 (PI-3). By viral neutralizaion test, three pathogens including BVD virus, BHV-1, PI-3 were applied for screening each neutralizing antibody and its titration. Among 948 sera, there were 215 seropositives (22.7%) against BVD virus, 25 (2.6%) against BHV-1 virus, and 161 (17.0%) against PI-3 virus, respectively. In the seropositive cases to BVD virus, provincial prevalence of them were recorded as Gyeongnam(30.8%) for the highest one, while as Jeonbuk (15.8%) and Gyeongbuk(l6.7%) relatively for the lower ones. Among them, higher than 1:8 in antibody titration was classified as 74 cases (34.4%) which were suspected the cycle of its infection in their herds. Among the seropositives to BHV-1, it was relatively lower prevalence rate ranging from 1.7% to 6.7% that those of BVD virus and PI-3, but more importantly they were prevailing across the country. Most of the seropositive cases (42.9%) to PI-3 were classified as 1:4-1:16 in titration, but also distributed all around the country. The prevalence in goats varied according to areas (15.8 to 30.8%). Related to the provincial prevalence of three viral agents in Korean goats, it seemed that three viral pathogens would be directly or indirectly involving in various disorders as primary or secondary causative agents resulting in decreased productivity.

한국 재래 산양에서 Leptospira spp.에 대한 혈청역학적 연구

한동운,권용국,문진산,윤소라,위성환,장환,탁동섭,이태욱,강문일 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.3

Between January 1999 and September 2001, 948 sera (120 from each province except 108 from Chungbuk) from 8 provinces were sampled from Korean goats. All the samples were serologically tested for Leptospira spp. By the microscopic agglutination test against four L. interrogans serovars including canicola, icterohaemorrhagiae, pomona and hardjo, 948 Korean goats were confirmed as 11 seropositives (1.2%) only infected with L. icterohemorrhagiae. All positive sera were collected from Gyeonggi (5), Gangwon (4) and Chungbuk (2). Titration of them were classified as 1:100 of 10 sera except one case with 1:200 from Gyeonggi. It was meaningful that the pathogen in Korean goats was mainly distributed in the northen part of the country. Consequently, the results in the study were confirmed and analyzed the recent status of infection of leptospirosisas zoonotic agentsome in Korean goats.

Immobilized metal ion affinity chromatography(IMAC)를 이용한 His-tagged B^30-homoserine 인슐린 전구체의 rapid one-step 분리 정제

한성욱,김동일,조정우 경운대학교 산업기술연구소 2000 産業技術硏究論文誌 Vol.3 No.1(B)

To purify His tagged B^30-homoserine insulin precursor from recombinant E. coli using a rapid single-step purification method, immobilized Ni^2+ -affinity chromatography which has been widely used to purify genetically fused to the interest protein was used. His tagged B^30-homoserine insulin precursor, molecular weight 9KDa, expression was accomplished by IPTG induction on pellet centrifuged of flask and batch broth and confirmed by SDS-PAGE either. It showed that recombinant protein is expressed as inclusion body. His-tagged B^30-homoserine insulin precursor which chelated with Ni^2+ metal ion was eluted lX Elution buffer, pH7.9 including 6M Urea with 1000mM imidazole. His tagged B^30-homoserine insulin precursor band was ascertained by SDS-PAGE with freeze dried powder obtained from lX Elution buffer extract.

A Study on the Comparison of wind pressure on the member of Container Crane using Wind tunnel test and CFD

한근조,안태원,이성욱,한동섭 동아대학교 생산기술연구소 2006 生産技術硏究所硏究論文集 Vol.11 No.-

Because strong wind is one of the few forces that, although considered in container crane design, still cause significant damage, a container crane was tested to investigate wind load characteristic in uniform flows. So, this study measured an external point pressure at the each members of a container crane according to a wind direction and a shape of members in a wind-tunnel test. The result of this test was compared to those of computation fluid dynamics using a CFX 10. The scale of a container crane model for wind tunnel test applied similarity scales to consider the size of the wind tunnel test section and the boundary condition for CFD is like wind tunnel test.

경북 지역의 지하수 수질에 관한 연구 4 : 암반 종류에 따른 지하수 수질 특성 The Characterisrics of Groundwater by Rock Types

한성욱,이동섭,김복조 경운대학교 산업기술연구소 2002 産業技術硏究論文誌 Vol.5 No.1(B)

We investigated the contamination of groundwater in Gyeongbuk region about 180 points by rock types. From the view point of rock types, the obtained results were as follows; The mean depth of rock types were decreased as follows sedimentary > gneiss > granite > granodiorite. In case of pH, the difference of value was small. While, the concentration of COD showed that it's concentration was increased as the mean depth was increased. In the region of granite, sedimentary and gneiss, the value of nitrate nitrogen was 3.5mg/l, 3.3mg/l and 1.7mg/l, respectively. In case of chloride ion, the concentration was highest for granite as 30.5mg/l and was medium for sedimentary and gneiss as 29.8mg/l and 26.9mg/l, respectively. while granodiorite was lowest as 8.97mg/l. The EC values were as follows; In the region of granite, sedimentary, gneiss and granodiorite, the value of EC was 444mg/l, 430mg/l, 390mg/l and 242mg/l, respectively.

양질의 수돗물 공급방안에 관한 연구

한성욱,김동일,문성덕 경운대학교 산업기술연구소 1999 産業技術硏究論文誌 Vol.2 No.1

The drinking water system has enabled us to provide a requested amount of water by a single water pipe wherever and whenever necessary, and it brings a lot of benefits to the people as well as the community concerned. The drinking water system is managed in principle, by water charge as the price of benefits brought by the system. The benefits are quite different according to the purpose of water use. Therefore, if the drinking water system can not bring various kinds of benefits to the people and community at the same time, its significance as a social infrastructure may be diminished.

악성 위궤양의 형태를 보인 원발성 위결핵 1예

한종대,이동욱,심성준,류운석,안현택,박찬욱,김배영,이미선 大韓消化器內視鏡學會 1999 Clinical Endoscopy Vol.19 No.부록2