농촌관과에 대한 지역주민의 인식에 관한 연구

곽대영 남서울대학교 2019 남서울대학교 논문집 Vol.25 No.-

여행업 종사자의 교육훈련 참여제약에 관한 연구

곽대영 남서울대학교 2016 남서울대학교 논문집 Vol.22 No.-

소아 야뇨증에 대한 임상적 고찰

김승수,곽병곤,김영대,김우경,김영균,이종국 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Objectives: The aim of this study was to describe the clinical characteristics of enuretic children and to evaluate the efficacy of treatments. Methods: Our study subjects enrolled were 52 children with nocturnal enuresis, who visited outpatient clinic of pediatrics in Seoul Paik Hospital and Ilsan Paik Hospital, during the period from October 1998 to August 2004. The data of gender, age, family history, symptom, clinical type of enuresis and drug therapy were collected and analyzed retrospectively. Results: The male : female ratio was 2.25 : 1. The mean age at diagnosis was 6.8 years with an age range from 4.3 years to 12.5 years. Fourty five cases(86.5%) were diagnosed as primary enuresis and 7 cases(13.5%) were diagnosed as secondary enuresis. A family history of enuresis was noted in 5 cases(9.6%) including 4 cases with parental enuresis history. The accompanying urinary symptoms were urgency(11.5%), frequency (7.7%), and dysuria(2.0%). Conclusion: We found frequent occurrences of enuresis in boys and primary type and an occasional association with a family history of enuresis. The two main treatment modalities were behavior control with an alarm system and drug therapy with imipramine, desmopressin, or oxybutynin. We recommend continuous drug medications to control nocturnal enuresis along with encouragement.

대장균에서 재조합 Rat Guanine deaminase 유전자의 발현, 정제 및 분석

성연선,곽상준,박대성,김향원,이희영 단국대학교 1998 論文集 Vol.33 No.-

Guanine deaminase(EC, 3.5.4.3, ;Guanine aminohydrolase, GAH, GDA) catalyzes the deamination reaction of guanine to xanthine irreversibly. The cDNA encoding rat guanine deaminase had been isolated from a λZAPII rat brain expression library using antibody generated against purified rat guanine deaminase. Toe obtain recombinant GDA and analyze the property of catalytically conserved retion, here we expressed the recombinant GDA in E.coli and showed the retention of its catalytic activity similar to native rat GDA. To make the construct carrying coding region to GDA in pGEX4T prokaryotic expression vector, the region encompassing open reading frame of pBlue-GDA was PCR amplified and subcloned into pGEX4T prokaryotic expression vector with correct reading frame of fusion carrier glutathione S-transferase. After transformation to E.coli DH5a. The bacteria carrying pGEX-GDA was grwon in the condition of IPTG induction. The fusion protein GST-GDA was purified with GSH-sepharose affinity chromatography. The purified GST-GDA was subsequently digested with biotin-conjugated thrombin, and thrombin was removed by streptavidin agarose. Then sample were treated with GSH-sepharose affinity chromatography to remove the contaminant GST and GST-GDA. The purified recombinant GDA showed 70% of specific activity relative to that of purified rat GDA. The recombinant GDA showed identical molecular weight with rat GDA in SDS-PAGE.

親電子性 芳香族 置換反應의 轉移狀態에 關한 理論的 硏究

朴柄珏,郭庚度,宋寧大 嶺南大學校 基礎科學硏究所 1991 基礎科學硏究 Vol.11 No.-

The transition state for electrophilic aromatic substitution was estimated by EHMO and CNDO/2 method. The transition state has been found to be δ-complex like model rather than π-complex like model for the substitution of a hydrogen in benzene with both proton and brominum ion. The electronic energies of δ-complex like models revealed the parallelism between the observed rate constant and the number of the substituted methyl groups in benzene. Consequetly, it can be generally thought that electrophilic aromatic substitution proceeds via δ-complex like model.

활성탄소섬유의 제조에 관한 연구

박인수,안대영,곽철 慶南大學校 附設 工業技術硏究所 2003 硏究論文集 Vol.21 No.1

Polyacrylonitrile(PAN) 섬유를 이용하여 활성탄소섬유(Activated Carbon Fibre: ACF)를 제조하였다. 원료를 먼저 탄화시킨 후에, 이산화탄소 분위기에서 수행되는 물리적인 활성화 방법을 사용하였다. 활성화 온도는 850∼l,100℃로 선택하였다. 활성화 반응의 반응성은 Burn-off율을 계산하여 구하였으며, 생성된 ACF의 기공 특성은 액체 질소 흡착/탈착 실험을 수행하여 측정하였다. 활성화 온도가 1000℃ 이하인 경우에, 제조된 활성탄소섬유의 평균 기공 크기는 약 23 Å이었으며, Burn-off와 비표면적은 활성화 온도 및 활성화 시간에 따라서 각각 선형적으로 증가하였다. 이에 반하여, 활성화 온도가 1,000℃ 이상인 경우에는, 활성화 온도 및 활성화 시간에 따라서 Burn-off는 증가하지만 비표면적은 오히려 감소하였다. Polyacrylonitrile(PAN) fibre was used to prepare the activated carbon fibre(ACF). The PAN fibre was carbonized first, then the fibre was activated physically by using carbon dioxide at 850∼l,100℃. The reactivity of the fibre was determined in terms of the burn-off of the fibre and the pore characteristics of the prepared ACF were determined by the liquid nitrogen adsorption/desorption experiment. When the activation temperature was lower than 1,000℃, the mean pore size of the prepared ACF was 23 Å and the burn-off and the specific surface area of the prepared ACF were increased linearly with the activation temperature. On the contrary, when the activation temperature was higher than 1,000 ℃. the burn-off was increased linearly, but the specific surface was rather decreased with the activation temperature.

비글개에서 신원방우황청심원의 급성 및 아급성독성시험

성하정,권오경,방명주,곽형일,이진영,신대희,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

Single and 4 weeks oral administration of New wonbangwoohwangchungsimwon (NSCH) which was used l-muscone as substitutive material of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs (3 males and 3 females) in acute experiments were administered orally with sinlge dose of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of NSCH, 160 ㎎/㎏/day (low dosage group), 400 ㎎/㎏/day (middle dosage group), 1,000 ㎎/㎏/day (high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korea Food and Drug Administration (1996. 4. 16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ per oral for both male and females. In animals administered with NSCH, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign, urinalysis, eye examination, hematology, serum chemistry, organ weight and other fingings. No histological lesions were observed in both control and treatment groups. Above data strongly suggset that NSCH in beagle dogs is considered to be safe.

백서 간세포 핵소체 인산단백질 B23의 진화론적인 특성 규명

이혜정,윤상인,박주인,정진숙,김영대,김정만,곽종영,김인후 동아대학교의과대학 1997 동아의대학술지 Vol.9 No.1

반코마이신 내성 장내구균 분리 환자에서 황색포도알균의 내성

김동욱,신선혜,김혜진,류선,이선희,장철훈,김영대,곽임수 대한감염학회 2004 감염과 화학요법 Vol.36 No.1

목적 : Vancomycin-resistant enterococci (VRE)로부터 S. aureus로의 vacomycin 내성 유전자의 전파는 오래전부터 예견되어져 왔으며 큰 걱정거리로 남아있다. 최근에 vanA 유전자를 가지고 있는 vancomycin-resistant S. aureus 2주가 미국에서 분리되었다. 이 연구의 목적은 VRE를 보균하고 있는 환자들에서 S. aureus 보균양상과 항균제 내성을 알아보는데 그 목적이 있다. 재료 및 방법 : 2001년 1월부터 2001년 12월까지 부산대학교병원에 입원하였던 환자들 중 임상검체에서 VRE가 분리된 환자 7명과 2001년 9월부터 12월까지 외과 중 환자실에 입원하였던 환자들 중 VRE 감시배양을 시행하였던 20명의 환자들을 대상으로 하였다. 환자들은 전비공, 액와부, 회음부, 직장에서 매주 면봉으로 검체를 채취하였다. 선별배지에서 검체를 배양하여 S. aureus를 분리하였고 원판 확산법을 이용하여 methicillin 감수성을 측정하였다. Broth microdilution 법으로 vancomycin MIC를 측정하였다. 결과 : 총 27명에서 73회의 방문과 292회의 배양이 시행되었으며 총 67주의 S. aureus가 분리되었다. 이중 64주(95.5%)가 methicillin 내성이었다. 전비공 MRSA 보균율은 VRE를 보균하고 있었던 환자는 19명 중 11명(58%)에서, VRE를 보균하지 않았던 환자는 8명 중 3명(37.5%)으로 VRE 보균자에서 보균율이 더 높았다. 총 64주의 MRSA의 vancomycin MIC의 분포는 0.5-2㎍/mL였으며 2㎍/mL를 초과하는 균주는 발견되지 않았다. 64주 중 vancomycin MIC가 1㎍/mL인 균주가 54주(84.4%)로 가장 많았으며 2㎍/mL 6주(9.4%), 0.5㎍/mL 4주(6.3%)의 순이었다. 결론 : VRE 환자에서 S. aureus의 보균율은 비보균자 보다 높았으며 분리된 균주들은 대부분 MRSA였다. VRSA 균주는 발견되지 않았다. Background : The transfer of vancomycin resistance from vancomycin-resistant enterococci (VRE) to Staphylococcus aureus has been predicted. The purpose of this study is to determine the prevalence and antibiotics resistance of S. aureus among patients colonized with VRE. Methods : Between January 2001 and December 2001, a prospective study was performed at Pusan National University Hospital on 27 patients. Surveillance swabs from nasal cavity, axilla, perineum, and rectum were obtained at weekly intervals. Methicillin susceptability of S. aureus was determined by oxacillin disk diffusion test and minimum inhibitory concentration (MIC) for vancomycin by microdilution broth test. Results : Total of 292 swab cultures were performed and 67 S. aureus isolates were collected. 64 isolates (95.5%) were resistant to methicillin. The prevalence of nasal MRSA carrier in 19 patients colonized with VRE was higher than that in 8 patients not colonized with VRE (58% vs. 37.5%). In 64 MRSA isolates, MIC (㎍/mL) for vancomycin ranged from 0.5 to 2. No isolates with MIC >2 ㎍/mL were observed. MIC of 1 ㎍/mL was shown (observed) in 54 isolates, 2 ㎍/mL in 6 isolates, and 0.5 ㎍/mL in 4 isolates. Conclusion : The prevalence of S. aureus with colonization of VRE is higher than that without colonization of VRE. Most of S. aureus isolates were resistant to methicillin. VRSA isolates were not observed.

Step Pulse Shaping Technique for Nd:YAG Laser Using a Multi-Switching Method

Kwak, Su-Young,Park, Jin-Young,Kim, Su-Weon,Min, Byoung-dae,Chung, Hyun-ju,Kim, Hee-je The Korean Institute of Electrical Engineers 2004 KIEE International Transactions on Electrophysics Vol.4C No.2

Throughout manufacturing processes, pulse shaping is required for material processing and it is regarded as an important (actor according to the specific characteristics of materials. Therefore, this study suggests a highly appropriate pulse shaping technique using a multi-switching method. This is a pulse superposition method in which one flash lamp can consecutively turn on by the double switching of the discharging system. It is possible to construct a variety of pulse shapes and pulse widths by the consecutive trigger of the silicon-controlled rectifiers (SCR) of a PIC (program integrated circuit) one-chip microprocessor. The use of this technique can provide a number of advantages to people who require suitable pulse shaping for particular applications such as welding, cutting, and drilling.