Peroxiredoxin II promotes hepatic tumorigenesis through cooperation with Ras/Forkhead box M1 signaling pathway

Park, Y-H,Kim, S-U,Kwon, T-H,Kim, J-M,Song, I-S,Shin, H-J,Lee, B-K,Bang, D-H,Lee, S-J,Lee, D-S,Chang, K-T,Kim, B-Y,Yu, D-Y Macmillan Publishers Limited 2016 Oncogene Vol.35 No.27

<P>The current study was carried out to define the involvement of Peroxiredoxin (Prx) II in progression of hepatocellular carcinoma (HCC) and the underlying molecular mechanism(s). Expression and function of Prx II in HCC was determined using H-ras(G12V)-transformed HCC cells (H-ras(G12V)-HCC cells) and the tumor livers from H-ras(G12V)-transgenic (Tg) mice and HCC patients. Prx II was upregulated in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg mouse tumor livers, the expression pattern of which highly similar to that of forkhead Box M1 (FoxM1). Moreover, either knockdown of FoxM1 or site-directed mutagenesis of FoxM1-binding site of Prx II promoter significantly reduced Prx II levels in H-ras(G12V)-HCC cells, indicating FoxM1 as a direct transcription factor of Prx II in HCC. Interestingly, the null mutation of Prx II markedly decreased the number and size of tumors in H-ras(G12V)-Tg livers. Consistent with this, knockdown of Prx II in H-ras(G12V)-HCC cells reduced the expression of cyclin D1, cell proliferation, anchorage-independent growth and tumor formation in athymic nude mice, whereas overexpression of Prx II increased or aggravated the tumor phenotypes. Importantly, the expression of Prx II was correlated with that of FoxM1 in HCC patients. The activation of extracellular signal-related kinase (ERK) pathway and the expression of FoxM1 and cyclin D1 were highly dependent on Prx II in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg livers. Prx II is FoxM1-dependently- expressed antioxidant in HCC and function as an enhancer of Ras(G12V) oncogenic potential in hepatic tumorigenesis through activation of ERK/FoxM1/cyclin D1 cascade.</P>

Nondegenerate n-type doping phenomenon on molybdenum disulfide (MoS₂) by zinc oxide (ZnO)

Kang, D.H.,Hong, S.T.,Oh, A.,Kim, S.H.,Yu, H.Y.,Park, J.H. Pergamon Press 2016 Materials research bulletin Vol.82 No.-

<P>In this paper, we have demonstrated nondegenerate n-type doping phenomenon of MoS2 by ZnO. The ZnO doping effects were systematically investigated by Raman spectroscopy and electrical/optical measurements (I-D-V-G with/without exposure to 520, 655, 785, and 850 nm laser sources). The ZnO doping improved the performance parameters of MoS2-based electronics (Ion up arrow, mu(FE)up arrow, n up arrow) owing to reduction of the effective barrier height between the source and the MoS2 channel. We also monitored the effects of ZnO doping during exposure to air; reduction in Delta V-TH of about 75% was observed after 156 h. In addition, the optoelectronic performance of the MoS2 photodetector was enhanced due to the reduction of the recombination rate of photogenerated carriers caused by ZnO doping. In our results, the highest photoresponsivity (about 3.18 x 10(3) A/W) and detectivity (5.94 x 10(12) Jones) of the ZnO-doped photodetector were observed for 520 nm laser exposure. (C) 2016 Elsevier Ltd. All rights reserved.</P>

Topiramate Improves Neuroblast Differentiation of Hippocampal Dentate Gyrus in the d-Galactose-Induced Aging Mice via Its Antioxidant Effects

Shen, H.,Wang, J.,Jiang, D.,Xu, P.,Zhu, X.,Zhang, Y.,Yu, X.,Won, M. H.,Su, P. Q.,Yan, B. C. Springer Science + Business Media 2017 Cellular and molecular neurobiology Vol.37 No.5

<P>Some anticonvulsant drugs are associated with cognitive ability in patients; Topiramate (TPM) is well known as an effective anticonvulsant agent applied in clinical settings. However, the effect of TPM on the cognitive function is rarely studied. In this study, we aimed to observe the effects of TPM on cell proliferation and neuronal differentiation in the dentate gyrus (DG) of the d-galactose-induced aging mice by Ki-67 and doublecortin (DCX) immunohistochemistry. The study is divided into four groups including control, d-galactose-treated group, 25 and 50 mg/kg TPM-treated plus d-galactose-treated groups. We found, 50 mg/kg (not 25 mg/kg) TPM treatment significantly increased the numbers of Ki-67(+) cells and DCX immunoreactivity, and improved neuroblast injury induced by d-galactose treatment. In addition, we also found that decreased immunoreactivities and protein levels of antioxidants including superoxide dismutase and catalase induced by d-galactose treatment were significantly recovered by 50 mg/kg TPM treatment in the mice hippocampal DG (P < 0.05). In conclusion, our present results indicate that TPM can ameliorate neuroblast damage and promote cell proliferation and neuroblast differentiation in the hippocampal DG via increasing SODs and catalase levels in the d-galactose mice.</P>

IL-32γ inhibits cancer cell growth through inactivation of NF-κB and STAT3 signals

Oh, J H,Cho, M-C,Kim, J-H,Lee, S Y,Kim, H J,Park, E S,Ban, J O,Kang, J-W,Lee, D-H,Shim, J-H,Han, S B,Moon, D C,Park, Y H,Yu, D-Y,Kim, J-M,Kim, S H,Yoon, D-Y,Hong, J T Nature Publishing Group 2011 Oncogene Vol.30 No.30

<P>Several studies have shown physiological functions of interleukin (IL)-32, a novel cytokine. However, the role of IL-32 in cancer development has not been reported. In this study, we showed that IL-32γ inhibited tumor growth in IL-32γ-overexpressing transgenic mice inoculated with melanoma as well as colon tumor growth in xenograft nude mice inoculated with IL-32γ-transfected colon cancer cells (SW620). The inhibitory effect of IL-32γ on tumor growth was associated with the inhibition of constitutive activated nuclear transcription factor-κB (NF-κB) and of signal transducer and activator of transcription 3 (STAT3). The expression of antiapoptotic, cell proliferation and tumor-promoting genes (<I>bcl-2</I>, <I>X-chromosome inhibitor of apoptosis protein</I> (<I>IAP</I>), <I>cellular IAP</I> and <I>cellular FADD-like IL-1β-converting enzyme-inhibitory protein</I>, <I>cyclin D</I>), cyclin-dependent kinase 4, cycolooxygenase-2 and inducible nitric oxide synthase was decreased, whereas the expression of apoptotic target genes (<I>caspase-3</I> and <I>-9</I>, <I>bax</I>) increased. In tumor, spleen and blood, the number of cytotoxic CD8<SUP>+</SUP> T cells and CD57<SUP>+</SUP> natural killer cells and the levels of IL-10 increased, but that of tumor necrosis factor-α (TNF-α), IL-1β and IL-6 decreased. We also found that forced overexpression of IL-32γ inhibited colon cancer cell (SW620 and HCT116) growth accompanied with the inhibition of activated NF-κB and STAT3 <I>in vitro</I>. In addition, when IL-32γ was knocked down by small interfering RNA (siRNA) or neutralized with an anti-IL-32γ antibody, IL-32γ-induced colon cancer cell growth inhibition, the IL-32γ-induced decrease of TNF-α, IL-1 and IL-6 production, and the increase of IL-10 production were abolished. However, siRNA of NF-κB and STAT3 augmented IL-32γ-induced colon cancer cell growth inhibition. These findings indicate significant pathophysiological roles of IL-32γ in cancer development.</P>

Characterization and Formation Mechanism of Ni3Si–Al2O3 Nanocomposite Prepared by Mechanochemical Reduction Method

H. Chen,D. M. Zhou,L. Cai,Y. Y. Wang,K. Yu 대한금속·재료학회 2020 METALS AND MATERIALS International Vol.26 No.2

In this present work, Ni3Si–Al2O3 nanocomposite powders were synthesized by mechanical milling using NiO, Si and Alas raw materials. The phase transformation, formation mechanism and microstructure evolution of the powders duringmechanical milling were investigated by X-ray difraction (XRD), diferential thermal analysis (DTA), scanning electronmicroscopy (SEM), transition electron microscopy (TEM) and microhardness measurements. Results showed that the Ni3Si,Al2O3 and Ni31Si12 phases formed after 5 h of milling with a rapid mechanically induced self-propagating synthesis mode. The average grain size and internal strain of Ni3Si and Al2O3 after 30 h of milling were (16.8 nm, 1.27%) and (19.6 nm,0.94%), respectively. The maximum microhardness value of 813 HV was obtained in the 30 h milled powder. The relationship between the hardness and grain size of the powders satisfes the Hall–Petch relationship. Ni3Si–Al2O3 nanocompositepowders are very stable during heating at 950 °C. By annealing of the milled powders leads to grain growth, internal strainand microhardness of Ni3Si powder decrease and transformation of disordered structure to an ordered state. A long-rangeordering parameter (LRO) of 0.97 for the ordered Ni3Si can be achieved after annealing at 950 °C for 2 h.

Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis

Yu, K‐,H.,Hong, K‐,S.,Lee, B‐,C.,Oh, M‐,S.,Cho, Y‐,J.,Koo, J‐,S.,Park, J‐,M.,Bae, H‐,J.,Han, M‐,K.,Ju, Y‐,S.,Kang, D‐,W.,Appelros, P. Blackwell Publishing Ltd 2011 Acta neurologica Scandinavica Vol.123 No.5

<P>Yu K‐H, Hong K‐S, Lee B‐C, Oh M‐S, Cho Y‐J, Koo J‐S, Park J‐M, Bae H‐J, Han M‐K, Ju Y‐S, Kang D‐W, Appelros P, Norrving B, Terent A. Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis. 
Acta Neurol Scand: 2011: 123: 325–331. 
© 2010 John Wiley & Sons A/S.</P><P><B>Background – </B> It has not been clarified whether the disparity in ischemic stroke outcome between populations is caused by ethnic and geographic differences or by variations in case mix. Propensity score matching (PSM) analysis can overcome some analytical problems but is rarely used in stroke outcome research. This study was to compare the ischemic stroke case‐fatality between two PSM cohorts of Sweden and Korea.</P><P><B>Methods – </B> Prognostic variables related to baseline characteristics and stroke care were included in our PSM model. Then, we selected 7675 Swedish and 1220 Korean patients with ischemic stroke from each stroke registers and performed one‐to‐one matching based on propensity scores of each patient.</P><P><B>Results – </B> After PSM, all measured variables were well balanced in 1163 matched subjects, and the 90‐day case‐fatality was identical 6.2% (HR 0.997, 95%CI 0.905–1.099) in Sweden and Korea.</P><P><B>Conclusions – </B> No difference is found in the 90‐day case‐fatality in propensity score‐matched Swedish and Korean patients with ischemic stroke.</P>

Peroxiredoxin II Is an Essential Antioxidant Enzyme that Prevents the Oxidative Inactivation of VEGF Receptor-2 in Vascular Endothelial Cells

Kang, D.,Lee, D.,Lee, K.,Park, Y.,Lee, J.,Lee, S.H.,Koh, Y.,Koh, G.Y.,Choi, C.,Yu, D.Y.,Kim, J.,Kang, S. Cell Press 2011 Molecular cell Vol.44 No.4

Cellular antioxidant enzymes play crucial roles in aerobic organisms by eliminating detrimental oxidants and maintaining the intracellular redox homeostasis. Therefore, the function of antioxidant enzymes is inextricably linked to the redox-dependent activities of multiple proteins and signaling pathways. Here, we report that the VEGFR2 RTK has an oxidation-sensitive cysteine residue whose reduced state is preserved specifically by peroxiredoxin II (PrxII) in vascular endothelial cells. In the absence of PrxII, the cellular H<SUB>2</SUB>O<SUB>2</SUB> level is markedly increased and the VEGFR2 becomes inactive, no longer responding to VEGF stimulation. Such VEGFR2 inactivation is due to the formation of intramolecular disulfide linkage between Cys1199 and Cys1206 in the C-terminal tail. Interestingly, the PrxII-mediated VEGFR2 protection is achieved by association of two proteins in the caveolae. Furthermore, PrxII deficiency suppresses tumor angiogenesis in vivo. This study thus demonstrates a physiological function of PrxII as the residential antioxidant safeguard specific to the redox-sensitive VEGFR2.

環境汚染에 關한 硏究 : 大氣汚染에 關하여 about Air Pollution

權赫姬,金東君,崔德一,金基俊,金元圭,李千熙,兪根烈,崔在益,崔東植 國立保健硏究院 1975 국립보건연구원보 Vol.12 No.-

노인의 병원 선택 경험

김단비,김민지,김해솔,김희정,박윤선,손유경,송예진,유예림,이다예,이서영,이지현,강숙정,김부연 이화여자대학교간호학회 2020 이화간호학회지 Vol.- No.54

Purpose: The purpose of this descriptive study was to examine hospital choice factors among elderly patients to understand their experience and use as data to move towards senior-friendly hospitals. Methods: The individual in-depth interviews were conducted from August 13th to 18th, 2019. The participants consisted of eight senior citizens aged 65 and above that suffered from chronic illnesses and had regular hospital visits. Results: This study found that when elderly patients choose the hospital, they considered ‘awareness such as brand name of the hospital', ‘quality of medical service',‘convenience', ‘healthcare team / hospital employee', ‘personal experience', ‘children’s recommendation’ and etc,. The significant point was that all these factors were related to personal experiences from specific hospitals. Conclusion: This study analyzed the hospital choice factors of the elderly patients with high hospital utilization rates and found that the results were mainly affected by the distinct characteristics of elderly patients. The implications of this study are that we proposed further research directions and means for the improvement of the hospital. We suggest hospitals to increase labor allocation for elderly patients with difficulties dealing with unmanned systems such as kiosk and strengthen the role of healthcare providers as instructors for higher satisfaction.

Aqueous and dietary bioaccumulation of antibiotic tetracycline in D. magna and its multigenerational transfer

Kim, H.Y.,Jeon, J.,Hollender, J.,Yu, S.,Kim, S.D. Elsevier Scientific Pub. Co 2014 Journal of hazardous materials Vol.279 No.-

The potential bioaccumulation and distribution of antibiotics in non-target organisms have been inadequately studied in spite of their widespread occurrence in aquatic systems. We investigated the ability of tetracycline to bioaccumulate through aqueous and dietary routes in an aquatic organism, the freshwater crustacean Daphnia magna. D. magna was exposed to algal food (Pseudokirchneriella subcapitata) contaminated with tetracycline for dietary uptake. Tetracycline was transferred to D. magna more through aqueous uptake than through dietary uptake. The uptake rate constant of tetracycline for D. magna was k<SUB>in,water</SUB>=0.33+/-0.045 via the aqueous route and k<SUB>in,food</SUB>=0.16+/-0.012 via the dietary route for 1.0mgL<SUP>-1</SUP> tetracycline. Bioconcentration factors of 4.40+/-0.91Lkg<SUP>-1</SUP> and 3.66+/-0.50Lkg<SUP>-1</SUP> for 0.1 and 1.0mgL<SUP>-1</SUP> tetracycline were found for D. magna. The biomagnification factor of 0.19+/-0.04 indicates that magnification of tetracycline through the food web will not occur. The change in the internal concentration of the target compound was also studied for multigenerational (F1-F4) exposure. The internal concentration in D. magna showed a decreasing trend with increasing generations except for the parent generation. The bioaccumulation tendency showed a biphasic change in multigenerational exposure.