Study of decreased melanin production through p53 by heme oxygenase-1 inhibitor in normal human melanocytes

( Hee Sun Lim ),( Sun A Jin ),( Jee Bum Lee ),( Seong Jin Kim ),( Seung Chul Lee ),( Young Ho Won ),( Sook Jung Yoon ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Heme oxygenase (HO)-1 is induced by oxidative stress and plays important roles in anti-apoptosis and the rapid growth of several solid tumors. p53 has a central role in skin pigmentation and may impact onmelanoma at all stages. However, there has been little study of HO-1 in relation with p53 on melanin production Objectives: To know the effect of HO-1 on melanogenesis through p53 in normal human melanocytes Methods: Human melanocytes (hM) were primarily cultured from foreskin. After incubation, cells were rinsed twice with PBS then transfection with p53 siRNA Results: Melanin content was detected with ELISA and Western blot analysis and RT-PCR of tyrosinase, MITF were performed after ZnPP treatment and p53 transfection. After ZnPP treatment, melanin content was decreased, and tyrosinase and MITF protein levels were decreased. Tyrosinase and MITF mRNA levels were also decreased. However, melanin content and tyrosinase and MITF protein levels were increased after CoPP treatment. After p53 transfection, HO-1 expression was decreased when HO-1 stimulator was treated. In addition, melanin content was decreased, and tyrosinase expression was decreased in Western blot analysis Conclusion: These results suggest that melanogenesis is inhibited by ZnPP by decreased melanin production, tyrosinase and MITF protein and mRNA levels. Furthermore, those inhibitory effects of ZnPP may be dependent on p53 in normal human melanocytes. Therefore, HO-1 may play an important role in melanogensis

현행 중등학교 과학 실험 , 실습 교육 실태 조사 및 그 운영 진단 (Ⅱ) : 고등학교 과학 실험 , 실습 교육을 중심으로

김영호,이윤종,정원우,강용희,양승영,윤성효,안병호,윤일희,권용주,임성규,오철한,기우항,전명남,김중욱 한국과학교육학회 1998 한국과학교육학회지 Vol.18 No.3

본 연구는 96년도에 실시한 우리 나라 중학교 과학 실험·실습 교육의 실태 조사 연구에 따른 계속 연구의 일환으로 고등학교 과학 실험·실습 교육의 실태를 조사하기 위한 것이다. 연구내용은 고등학교 과학 교과서의 분석, 실험·실습 실시 현황 분석, 현행 실험·실습 교육의 문제점 분석, 현행 학교 구성원들의 실험·실습 교육 운영 실태 조사 및 현행 실험·실습 교육의 개선을 위한 연구과제 도출을 주요 연구내용으로 다루었다. 연구방법은 전국의 고등학교 80개교를 대상으로 이들 학교의 학생 1,977명, 교사 165명, 학교장 80명에게 적용하였다. 본 연구에서 밝혀진 결과를 중심으로 우리 나라 고등학교 과학 실험 실습 교육의 정상화를 위한 실험 내적인 연구과제를 제시하면 다음과 같다. (1)교재 개발에 대한 연구, (2)실험 킷트 개발에 대한 연구, (3)교사교육 및 재교육 프로그램 개발에 대한 연구, (4)교실 밖 실험·실습 활동 프로그램 개발에 대한 연구, (5)과학 실험·실습 소재의 개발에 대한 연구. This study was accomplished to analysis and survey on the experimental and practical science education of high school in korea for the consecutive study of the an analysis and survey on the experimental and practical science education of middle school in korea(Lee, Yoon-Jong et al., 1997). The status of facilities, management for the experiment, practices, teaching methods in high schools have been investigated. The present status and reasonable management of the high school science education have been grasped from the questionaires. To do this 165 high school science teachers, 1977 students and 80 principals of high schools in Korea are administered questionaires of Science Education Research Institute of Kyungpook National University(1997). The results of this study are as follows : The reasonable management for experiments and practices of science education were scanty in the high school around the urban and rural school owing to the shortage of facilities and equipments, crowded class, excessive class works for teacher, excessive contents of present textbooks and insufficiency of the administrative supports etc. The current teaching method of high school science has emphasized knowledge. This fact does not satisfy the objective of learning due to lack of the teaching method. Desirable directions for the improvement of present status of high school science education were proposed in this paper.

Anti-Inflammatory and Cytoprotective Effects of TMC-256C1 from Marine-Derived Fungus <i>Aspergillus</i> sp. SF-6354 via up-Regulation of Heme Oxygenase-1 in Murine Hippocampal and Microglial Cell Lines

Kim, Dong-Cheol,Cho, Kwang-Ho,Ko, Wonmin,Yoon, Chi-Su,Sohn, Jae Hak,Yim, Joung Han,Kim, Youn-Chul,Oh, Hyuncheol MDPI AG 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.4

<P>In the course of searching for bioactive secondary metabolites from marine fungi, TMC-256C1 was isolated from an ethyl acetate extract of the marine-derived fungus <I>Aspergillus</I> sp. SF6354. TMC-256C1 displayed anti-neuroinflammatory effect in BV2 microglial cells induced by lipopolysaccharides (LPS) as well as neuroprotective effect against glutamate-stimulated neurotoxicity in mouse hippocampal HT22 cells. TMC-256C1 was shown to develop a cellular resistance to oxidative damage caused by glutamate-induced cytotoxicity and reactive oxygen species (ROS) generation in HT22 cells, and suppress the inflammation process in LPS-stimulated BV2 cells. Furthermore, the neuroprotective and anti-neuroinflammatory activities of TMC-256C1 were associated with upregulated expression of heme oxygenase (HO)-1 and nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) in HT22 and BV2 cells. We also found that TMC-256C1 activated p38 mitogen-activated protein kinases (MAPK) and phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathways in HT22 and BV2 cells. These results demonstrated that TMC-256C1 activates HO-1 protein expression, probably by increasing nuclear Nrf2 levels via the activation of the p38 MAPK and PI3K/Akt pathways.</P>

인터넷 통신에서 자바 바이트코드를 이용한 애플릿 보안

윤찬호,석경휴,나기창,배철수,나상동 조선대학교 전자정보통신연구소 2002 電子情報通信硏究所論文誌 Vol.5 No.1

Java applets are downloaded from web server through internet and executed in Java Virtual Machine of clients' browser. Before execution of java applets, JVM checks bytecode program with bytecode verifier and performs runtime tests with interpreter. However, these tests will not protect against undesirable runtime behavior of java applets, such as denial of service attack, email forging attack, URL spoofing attack, or annoying sound attack. In order to protect malicious applets, a technique used in this paper is java bytecode modification. This technique is used to restrict applet behavior or insert code appropriate to profiling or other monitoring efforts. Java byte modification is divided into two general forms, class-level modification involving subclassing non-final classes and method-level modification used when control over objects from final classes or interface. This paper showed that malicious applets are controlled by java bytecode modification using proxy server. This implementation does not require any changes in the web sever, JVM or web browser.

수산화세륨(Ⅲ)으로부터 질산암모늄세륨(Ⅳ)의 제조

윤호성,김철주,이진영,김성돈,김준수,유승준 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.3

본 연구에서는 수산화세륨으로부터 질산세륨 용액 제조 후 질산암모늄 첨가에 의한 질산암모늄세륨 제조시, 출발용액의 산도와 농도 그리고 질산암모늄 첨가량, 반응온도 및 반응시간이 질산암모늄세륨 합성에 미치는 영향을 고찰하였으며, XRD, SEM 그리고 TGA 분석을 통하여 결정의 형태 및 구조에 대한 고찰을 수행하였다. 4가의 수산화세륨을 질산에 용해한 질산세륨(Ⅳ)수용액으로부터 질산암모늄 첨가에 의한 질산암모늄세륨 제조시 회수율을 고려한 최적 합성조건은 출발물질의 세륨 농도 70-100g/l, 출발용액의 산도 7 mol/l, 질산암모늄 첨가량 2.5당량, 감압·가열온도 65℃ 및 반응시간 15-30min.이었으며, 제조된 질산암모늄세륨의 결정 화학식은 (NH_4)_2[Ce(NO_3)_6) · ×H_2O(x=6-8)인 것을 알 수 있었다. Synthetic process of ammonium cerium nitrate from cerium hydroxide was presented and influences of concentration and acidity of starting solution, addition quantity of ammonium nitrate, reaction temperature, reaction time on the yield of ammonium cerium nitrate are discussed. The shape and composition of crystal were determined by SEM, thermogravimetry differential thermal, x-ray diffraction and chemical analysis. The optimum synthesizing conditions of ammonium cerium nitrate were discribed as follows; rare earth concentration of starting solution 70-100 g/l. acidity of starting solution 7 mol/l, addition quantity of ammonium nitrate 2-3 equivalent, the condition of rotary evaporation 65℃ and reaction time 15-30 min. It was also known that the chemical formulae of ammonium cerium nitrate was (NH_4)_2[Ce(NO_3)_6] ·×H_2O(x=6-8) from TGA.

부산시 지가와 기능공간 분포특성

윤철현,황영우,전호근 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.1

This paper has tried to get hold of spatial distribution of land value in Pusan and an influence of land value on the various kinds of urban functions. Conclusions by the research are as follows. First, points of peak land value in Pusan are Kwangbok-Dong and Bujeon-Dong, and Kwangbok-Dong is greater than Bujeon-Dong in the influential zone according to just spatial present condition of land value. Second, specialized functional agglomeration and regional differentiation functionally are explicit in the region of Kwangbok-Dong. Third, functions having great correlation with land value are high quality retail, food and entertainment, cultural, and service function. Finally, functions having interdependent relation with many other ones are official, food and entertainment, service, and cultural function.

TW-RBF 신경망을 이용한 음성인식에 관한 연구

이윤직,강철호 광운대학교 신기술연구소 1998 신기술연구소논문집 Vol.27 No.-

본 논문은 음성인식을 위해 기존의 RBF를 변형하여 TW(Time-Warping) 기능을 갖는 RBF (Radial Basis Function) 신경망을 제안한다 RBF 신경망은 은닉충외 각 노드에 중심점(center)이라는 벡터를 가지고 있으며 이 백터와 가중치(weight)의 학습방법에 따라 여러 가지 응용에 이용하고 있다. 본 논문에서는 중심점의 자율학습과 가중치의 지도학습을 통하여 화자독립 음성인식 시스템을 구성하였다. 학습 단계에서는 학습 단어의 프레임 수에 따라 은닉충 뉴런의 수를 결정 하였다. 학습시의 TW-RBF 신경망을 통하여 각각의 프레임별로 국부동조(locally tuned)된 중심점 백터를 구할 수 있었다. 인식 단계에서는 이 신경망을 2차원으로 배열하고 입력층과 은닉층을 완전결합하여 신경망의 출력이 2차원의 오차 패턴이 되도록 하였다. 이러한 오차 패턴에Dynamic Programming 기법을 도입하여 최적경로를 찾게 하였다. 컴퓨터 모의 실험에 사용한 단어는 데이터 Ⅰ로 "공, 하나, 둘 .. 아흡"의 10 단어와 데이터 Ⅱ로 "영. 하나. 둘‥‥ 구"의 10 단어로 총 숫자음 20단어이다. 화자 독립의 경우 데이터 Ⅰ에 대하여 평균 86.3%, 데이터 Ⅱ에 대하여 평균 93.8%, 전체 데이터 20개 단어에 대해서는 평균 82.5%의 인식율을 보여 제안한 TW-RBF 신경망이 TW 기능이 없는 RBF 신경망과 DTW에 비해 우수한 인식율을 나타내었다. In this paper the RBF(Radial Basis Function) neural network with TW(Time-Warping) function is proposed by modifying the existing RBF The RBF neural network has the center vector at each node of hidden layer. And it depends on the learning methods of center vectors and weights in many applications. The speaker-independent speech recognition system is designed by unsupervised learning of center vectors and supervised learning of weights. In the learning mode, the neuron number of hidden layer is determined by the frame number of learning words. Through the learning mode of TW-RBF, the updated weights and locally tuned center vectors are obtained by each frame. In the recognition mode, this neural network is arranged on 2-dimensional structure and fully connected between hidden layer and input layer. The output pattern of this neural network consists of 2-dimensional error pattern. At this pattern, the optimal path is determined by using Dynamic Programming method. In this paper it is tested for twenty numerical speech data ??(“Kong. Hana. Dul. ...A-hop"), data ?? ("Young, ??, ??, ...Ku"). In the speaker independent case, these experiments result in the recognition rate of everage 86.3% for data ?? 93.8% for data ??, 82.5% for total twenty data. It shows that the performance of the proposed TW-RBF is better than that of DTW and RBF without TW function.

실드터널 시공시의 지표 구조물의 침하 억제 대책에 관한 연구

임종철,윤이환,박이근,고호성,홍석우 釜山大學校生産技術硏究所 1999 生産技術硏究所論文集 Vol.56 No.-

연약지반 터널 굴차시 실드공법은 근접 구조물 안전에 유용한 공법이다. 하지만 실드 터널 공법은 터널 주변 지반의 변형을 최소화 할 수 있는 장점을 가지는 반면 테일 보이드(Tail Void)에 그라우트된 주입재가 경화되기 전에 발생하는 지반 변형은 피하기 어렵다. 본 연구에서는 테일 보이드로 인한 인접 구조물의 침하를 억제하는 방법중 하나인 마이크로파일(micropile)의 영향을 모형실험을 통하여 규명하였다. 기초적인 실험 결과로써 마이크로파일 보강시 마이크로파일의 효과적인 설치방향과 침하 억제비를 밝혔다. In soft ground tunneling, shield method is very good for safety of neighboring structures. Although shield tunnel method has the merits to minimize the deformation of ground around tunnel, ground deformations occurred until the material grouted in tail void hardens are inevitable. In this study, the effects of micropile used as one method to restrain the settlement of neighboring structures by the tail void are studied by laboratory model tests. As a basic test result, the effective direction of micropile and the restraint rate of settlement by micropile reinforcement are known.

고로슬래그미분말 대체율에 따른 고품질 콘크리트의 공학적 특성에 관한 실험적 연구

나철성,윤종기,장종호,김재환,유득현,김무한 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

As a part of efforts for conformity of demand in regard to high quality of concrete, blast-furnace slag has been utilized by means of cement replacement. Superior performance can be ensured, environmental pollution can be prevented and economical advantage can be obtained with utilization by cement placement. But the study on the blast-furnace slag is not systematic and reasonable. So, it is planed that basic data in regard to technique of manufacturing and economic improvement of high quality concrete is showed with experimental comparison and investigation of engineering properties of high quality concrete utilized by cement placement of blast-furnace as industry by-product in this study.

관상동맥 성형술이 QT 간격분산에 미치는 영향

신이철,김윤년,현대우,허승호,박남의 대한순환기학회 2003 Korean Circulation Journal Vol.33 No.11