진균혈증의 진단에 있어서 혈청 (1→3)-β-D-glucan 측정의 유용성

최석훈,한상훈,진범식,박윤선,조병철,김희만,김윤재,이꽃실,장경희,송영구,김준명 대한감염학회 2003 감염과 화학요법 Vol.35 No.4

목적 : 진균혈증의 신속한 진단은 진균혈증 치료의 핵심이다. 그러나 진균혈증의 전통적인 진단법은 어렵기 때문에 진단을 지연시킬 수 있다. 진균혈증의 진단에서 β-glucan의 임상적 유용성을 알아보기 위하여 본 연구자들은 진균혈증 환자의 혈청 β-glucan의 농도를 측정하기 위하여 대조군으로써 균혈증 환자와 건강한 성인과 비교하였다. 방법 : 2001년 8월에서 2002년 10월까지 16명의 진균혈증 환자, 13명의 균혈증 환자, 그리고 18명의 정상 성인의 혈청 β-glucan 농도를 측정하였다. 본 연구자들은 β-glucan 농도를 β-Glucan Test(Wako Pure Chemical Industries, Ltd., Osaka, Japan)를 이용하여 측정하였다. 진균혈증 환자와 다른 환자들 사이의 혈청 β-glucan 농도 비교는 t-검정을 이용하였다. 결과 : 진균혈증 환자 중에서 남자는 10명(62.5%)이었고, 여자는 6명(37.5%)이었으며, 평균연령은 52.9±16.2세였다. 균혈증 환자 중에서 남자는 6명(46.2%)이었고, 여자는 7명(53.8%)였으며, 평균연령은 52.3±17.1세였다. 정상 성인 중에서 남자는 15명(83.3%)이었고, 여자는 3명(16.7%)였다. 평균연령은 23.8±2.1세였다. Cut-off value는 11 pg/mL이었다. 총 16명의 진균혈증 환자 중에서 13명의 혈청 β-glucan의 농도가 cut-off value 이상이었고(범위:11.5-863 pg/mL, 민감도:81.3%, 특이도:100%), 평균 농도는 217.8±273.8 pg/mL이었다. 총 13명의 균혈증 환자의 혈청 β-glucan 농도는 전부 cut-off 이하였고, 평균 혈청 β-glucan 농도는 0.1±0.3pg/mL이었다. 총 18명의 정상 성인의 혈청 β-glucan의 농도는 전부 cut-off value 이하였고, 평균 혈청 β-glucan 농도는 0 pg/mL이었다. 진균혈증 환자의 혈청 β-glucan의 농도는 통계학적으로 유의하게 대조군인 균혈증 환자와 건강한 성인의 농도보다 높았다(P-value : 0.006 및 0.006). 결론 : 결론적으로 진균혈증 환자의 진단에서 혈청 β-glucan의 농도 측정은 임상적으로 의미가 있다. 그러나 본 연구가 임상적으로 더욱 유용하기 위하여는 혈청 β-glucan 농도 측정을 통한 진균혈증의 조기진단이 진균혈증 환자의 예후에 미치는 영향과 혈청 β-glucan 농도와 진균혈증 환자의 항진균제에 대한 치료반응과의 연관을 밝히는 연구가 진행되어야 할 것이다(본 연구는 WAKO사의 β-glucan Test (Wako Pure Chemical Industries, Ltd., Osaka, Japan)의 제공으로 이루어졌음) Background : Early recognition of fungemia is essential for successful treatment. However, methods to culture fungus specimen taken from fungemia patients are difficult and time consuming. To assess the clincal usefulness of β-D-glucan in the detection of fungemia. we compared serum (1→3)-β-D-glucan concentrations in fungemia, bacteremia, and healthy persons. Methods : From August 2001 to October 2002, serum (1→3)-β-D-glucan concentrations were measured by turbidometric assay in 16 fungemia patients, 13 bacteremia patients and 18 healthy persons. Differences in (1→3-β-D-glucan concentrations between fungemia patients and other groups were compared by t-test. Results : Fungemia patients were composed of 10 male and 6 female patients, and mean age was 52.9±16.2 years. The cut-off value for a positive result was 11 pg/mL. thirteen out of 16 fungemia patients had concentrations above the cul-off value (range: 11.5-863 pg/mL, sensitivity: 81.3%, specificity: 100%), and mean concentration in fungemia was 217.8±273.8 pg/mL. Mean concentration in bacteremia was 0.1±0.3 pg/mL, and all the patients with bacteremia had the concentrations below the cut-off value. Mean concentration in the healthy persons was 0 pg/mL and all healthy persons had concentration below the cut-off value. The concentration in fungemia was statistically significantly higher than those of the other two groups(p-value: respectively 0.006, 0.006) Conclusion : We concluded that serum (1→3)-β-D-glucan is useful for the diagnosis of fungemia. Further study on the usefullness of serum (1→3)-β-D-glucan for early detection of fungemia and therapeutic monitoring is warranted.

이종장기이식에 의한 면역거부반응에서 IL-18의 역할

명평근,최연실,심정현,김은미 충남대학교 생물공학연구소 2003 생물공학연구지 Vol.9 No.2

Although transplantation immunology as a distinctive field began with the development of experimental models that showed the feasibility of bone marrow transplantation (BMT), organ engraftment was accomplished first in humans, and was though for many years to occur by drastically different mechanism. The liver, skin, and gastrointestinal tract are major target organs of acute graft-versus-host disease (GVHD), the major complication of allogenic BMT. The pathophysiology of acute GVHD involves in the dysregulation of pro-inflammatory cytokine cascade and donor T-cell responses to host alloantigens. Interukine-18 (IL-18) was initially known as interferon-g-inducing factor with potent immunomodulatory effects. The level of IL-18 is increased in acute GVHD, but little has been known about its role in thepathophysiology of acute GVHD. It reduces the severity of acute GVHD as a Thl-inducing cytokine early after BMT to the lethally irradiated recipients. When administered to the donor, it can also reducing the severity of acute GVHD, as a Th2-inducing cytokine. Therefore, IL-18 has the remarkable capacity to modulate acute CVHD when administered either to the donor or the recipient through distinct mechanism. Here, we present our view of the concepts of transplantation immunology and the role of IL-18 in the graft rejection.

Castleman disease of the abdomen

Myung-Hw Kim,Shin Hwang,Youn-Baik Choi,Sung-Tae Oh,Song-Cheol Kim,Eun-Sil Yu,Sung-Gyu Lee 대한외과학회 2010 대한외과학회 학술대회 초록집 Vol.2010 No.11

신 및 신주위 농양의 최근 임상 경향

최준용,김명수,김영근,이꽃실,장경희,허애정,염준섭,송영구,김준명 대한화학요법학회 2002 대한화학요법학회지 Vol.20 No.2

목적 : 신 및 신주의 농양의 최근 임상 양상에 대해 알아보고자 하였다. 방법 : 1997년 1월부터 2001년 6월까지 세브란스 병원에서 신농양 및 신주의 농양으로 진단 받은 34예를 대상으로 연령 분포, 성별, 입원 전 증상의 지속 기간, 검사실 소견, 원인균, 유발인자, 방사선 소견 및 진단과 치료 등에 대하여 후향적으로 조사하였다. 결과 : 신농양은 16예, 신주위 농양은 18예였다. 평균 연령은 52.9±12.9(19-82세)세였고 성별비는 1:1.4였다. 기저 상태로는 당뇨 18례(52.9%), 간질환 7례(20.5%), 스테로이드 복용 5례(14.7%), 악성종양 3례(8.8%), 신경원성 방광 2례(5.8%), 다낭신 2례(5.8%), 신결석 1례(2.9%) 등이 있었다. 입원 시 증상은 발열(82.3%)과 측복부 동통(67.6%)이 대부분이었다. Escherichia coli, Klebsiella pneumoniae 등의 그람 음성균이 가장 흔한 원인균이었다. 신 및 신주위 농양의 치료에 있어 항생제 치료, 경피적 배농, 수술적 치료가 재원 기간, 치료 기간 등의 치료 양상에 있어 유사한 결과를 나타냈다. 신농양과 신주위 농양간에 임상적 특성은 유의한 차이를 나타내지 않았다. 결과 : 본 연구를 통해 신 및 신주위 농양에서 원인균, 치료방법, 예후 등의 임상 양상이 외국의 보고와 비슷한 양상으로 변화했다는 점을 확인할 수 있었고, 향후 신 및 신주위 농양의 치료에 있어 비수술적인 치료 방법의 치료 효과에 대한 보다 많은 예에서의 전향적인 연구가 필요하리라 생각된다. Background : The clinical aspects of renal or perirenal abscess has changed over the decades. In Korea, there were no recent reports about clinical aspects of renal or perirenal abscesses. Methods : We retrospectively reviewed charts of 34 patients diagnosed as renal or perirenal abscesses about age, sex, clinical manifestations, laboratory findings, culture studies, underlying conditions, radiologic findings, treatment modalities, duration of treatment, and treatment outcomes. Results : A total of 54 patients with renal or perirenal abscess was identified. The mean age of overall patients was 52.9?12.9 years. Underlying conditions were diabetes mellitus(52.9%), liver disease(20.5%), steroid use(14.7%), malignancy(8.8%), neurogenic bladder(5.8%), polyeystic kidnev(5.8%) and renal stone(2.9%). Common clinical manifestations were fever(82.3%) and flank pain(67.6%). Gram negative strains such as Escherichia coli, Klebsiella pneumoniae were common causative organisms. Mean size of abscess was 5.27 ±3.05㎝(0.5-13.0㎝). There were no significant differences in the hospital days or treatment duration between treatment modalities such as antibiotics, percutaneous drainage and surgery. There were no significant differences in the clinical characteristics between renal and perirenal±renal abscess. Conclusion : In our study, there were changes of clinical characteristics such as causative organisms or treatment modalities similar with that in foreign countries. The prospective study should be processed about treatment outcomes of noninvasive treatment in renal or perirenal abscess.

Proteomic Analysis and the Antimetastatic Effect of N-(4-methyl)phenyl-O-(4-methoxy)phenyl-thionocarbamate-Induced Apoptosis in Human Melanoma SK-MEL-28 cells

Choi, Su-La,Choi, Yun-Sil,Kim, Young-Kwan,Sung, Nack-Do,Kho, Chang-Won,Park, Byong-Chul,Kim, Eun-Mi,Lee, Jung-Hyung,Kim, Kyung-Mee,Kim, Min-Yung,Myung, Pyung-Keun 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

We employed human SK-MEL-28 cells as a model system to identify cellular proteins that accompany N-(4-methyl)phenyl-O-(4-methoxy)phenyl-thionocarbamate (MMTC)-induced apoptosis based on a proteomic approach. Cell viability tests revealed that SK-MEL-28 skin cancer cells underwent more cell death than normal HaCaT cells in a dose-dependent manner after treatment with MMTC. Two-dimensional electrophoresis in conjunction with matrix-assisted laser desorption/ionization-time of flight (MALDl- TOF) mass spectrometry analysis or computer matching with a protein database further revealed that the MMTC-induced apoptosis is accompanied by increased levels of caspase-1, checkpoint suppressor-1, caspase-4, NF-κB inhibitor, AP-2, c-Jun-N-terminal kinase, melanoma inhibitor, granzyme K, G1/S specific eye/in D3, cystein rich protein, Ras-related protein Rab-37 or Ras-related protein Rab-13, and reduced levels of EMS (oncogene), ATP synthase, tyrosine-phosphatase, Cdc25c, 14-3-3 protein or specific structure of nuclear receptor. The migration suppressing effect of MMTC on SK-MEL-28 cell was tested. MMTC suppressed the metastasis of SK-MEL-8 cells. It was also identified that MMTC had little angiogenic effect because it did not suppress the proliferation of HUVEC cell line. These results suggest that MMTC is a novel chemotherapeutic and metastatic aoents aqainst the SK-MEL-28 human melanoma cell line.

Proteomic Analysis and the Antimetastatic Effect of N-(4methyl)phenyl-O-(4-methoxy) phenyl-thionocarbamate-Induced Apoptosis in Human Melanoma SK-MEL-28 cells

Choi Su-La,Choi Yun-Sil,Kim Young-Kwan,Sung Nack-Do,Kho Chang-Won,Park Byong-Chul,Kim Eun-Mi,Lee Jung-Hyung,Kim Kyung-Mee,Kim Min-Yung,Myung Pyung-Keun The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.3

We employed human SK-MEL-28 cells as a model system to identify cellular proteins that accompany N-(4-methyl)phenyl-O-(4-methoxy)phenyl-thionocarbamate (MMTC)-induced apoptosis based on a proteomic approach. Cell viability tests revealed that SK-MEL-28 skin cancer cells underwent more cell death than normal HaCaT cells in a dose-dependent manner after treatment with MMTC. Two-dimensional electrophoresis in conjunction with matrixassisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis or computer matching with a protein database further revealed that the MMTC-induced apoptosis is accompanied by increased levels of caspase-1, checkpoint suppressor-1, caspase-4, NF-kB inhibitor, AP-2, c-Jun-N-terminal kinase, melanoma inhibitor, granzyme K, G1/S specific cyclin D3, cystein rich protein, Ras-related protein Rab-37 or Ras-related protein Rab-13, and reduced levels of EMS (oncogene), ATP synthase, tyrosine-phosphatase, Cdc25c, 14-3-3 protein or specific structure of nuclear receptor. The migration suppressing effect of MMTC on SK-MEL-28 cell was tested. MMTC suppressed the metastasis of SK-MEL-8 cells. It was also identified that MMTC had little angiogenic effect because it did not suppress the proliferation of HUVEC cell line. These results suggest that MMTC is a novel chemotherapeutic and metastatic agents against the SK-MEL-28 human melanoma cell line.

돼지 신장세포(PK15)의 이종이식 거부반응에 대한 재조합 IL-18의 영향

최연실,김은미,김영관,박상연,심정현,윤도영,강형식,명평근 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

Various cytokines including IL-1β, IL-2, IL-4, IL-10, IL-12p40, IFN-r, and TNF-α have been recently reported to play important roles in both host and donor cell activations during xenotransplantation. Interleukin (IL)-18, a proinflammatory cytokine, can induce IL-4 and IL-13 production in T cells, NK cells, B cells, mast cells, and basophils. IL-18 has the capacity to stimulate innate immunity and both Th1-and Th2- mediated responses, but little is known to involve in the xenograft rejection. In order to investigate the role of IL-18 in xenograft rejection, we transplanted the pig kidney (PK15) cells to C57BL/6 mouse with or without intraperitoneal injection of mouse recombinatant IL-18, It was analyzed the population of T cell, B cell and NK cell in the mice transplanted with PK 15 cells and recombinant IL-18 by flow cytometry. We found that splenic CD3+ T cells were increased in mice injected PKl5 cells with recombinant IL-18. These results suggest that recombinant IL-18 is critical in the xenograft rejection by increasing T cell population of the recipients.

Fluconazole-Resistant <i>Candida parapsilosis</i> Bloodstream Isolates with Y132F Mutation in <i>ERG11</i> Gene, South Korea

Choi, Yong Jun,Kim, Yae-Jean,Yong, Dongeun,Byun, Jung-Hyun,Kim, Taek Soo,Chang, Yun Sil,Choi, Min Ji,Byeon, Seung Ah,Won, Eun Jeong,Kim, Soo Hyun,Shin, Myung Geun,Shin, Jong Hee U.S. Department of Health and Human Services * Cen 2018 Emerging Infectious Diseases Vol.24 No.9

<P>We recently observed the emergence of fluconazole-resistant <I>Candida parapsilosis</I> bloodstream isolates harboring a Y132F substitution in Erg11p in South Korea. These Y132F isolates had a higher propensity to cause clonal transmission than other fluconazole-resistant isolates and persisted within hospitals for several years, as revealed by microsatellite typing.</P>

Electromagnetic Interference Shielding Effectiveness of Electroless Nickel-plated MWCNTs/CFs-reinforced HDPE Matrix Composites

Choi, Woong-Ki,Hong, Myung-Sun,Lee, Hae-Seong,An, Kay-Hyeok,Bang, Joon-Hyuk,Lee, Young Sil,Kim, Byung-Joo Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.2

In this work, the electromagnetic interference shielding effectiveness (EMI-SE) of carbon nanotube/carbon fiber-reinforced HDPE matrix composites are investigated with various preparation conditions, such as the carbon fiber and carbon nanotube content, the presence of metal additives, as well as mixing speed and time. It was found that the EMI-SE of the composites increased with filler contents and metal additives. These results indicate that the content and length of carbonaceous fillers determine the electric networks in the composites, resulting in the control of the EMI-SE of the composites.

Immunosuppression of xenograft rejection in porcine kidney PK15 cells by porcine IL-18

Choi, Yon Sil,Kim, Young Kwan,Shim, Jung Hyun,Kim, Eun Mi,Kang, Hyung Sik,Yoon, Do Young,Muneta, Yoshihiro,Myung, Pyung Keun 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

Xenotransplantation, the transplantation of cells, tissues or organs between individuals of different species, would resolve the current shortage of organs, but rejection remains the major hurdle to successful xenotransplantation. In the present study, we analyzed mixed lymphocyte reactions (MLRs) and used (51)^Cr release assays in order to identify the proliferation and expansion of mouse CD8^(+) cytotoxic T lymphocyte cells against PK15, PK15/pIL-18 or PK15/mlL-18 cells. In addition, we identified T cell populations in mouse splenocytes and lymph node cells using two-color flow cytometry. It was found that the CD8^(+) T cells of xenograft recipients proliferated extensively and that the survival rates of populations of PK15/mlL-18 or PK15/plL-18 cells were higher than untransfected controls. Moreover, CD3^(+) T cells were Increased in mice injected with PK15 cells or PK15/plL-18 cells but PK15/plL-18 cell numbers were lower In lymph nodes than untransfected controls. CD8^(+) T cells numbers were reduced in the lymph nodes of PK15/plL-18 injected mice. These results suggest that porcine lL-18 regulates anti-pig cellular rejection In C57BL/6 mice.