Time-Related Changes in Detrusor Overactivity in Awake Rats with Spinal Cord Injury Observed by Simultaneous Registrations of Intravesical and Intraabdominal Pressures

Jin, Long-Hu,Shin, Hwa-Yoan,Yoon, Seung-Hwan,Seong, Do-Hwan,Park, Chang-Shin,Lee, Tack,Yoon, Sang-Min Korean Continence Society 2011 International Neurourology Journal Vol.15 No.1

<P><B>Purpose</B></P><P>To compare the physical characteristics of detrusor overactivity (DO) induced by intravesical infusion of saline in awake, sham rats and rats with chronic spinal cord injury (SCI), by simultaneous registrations of intravesical and intraabdominal pressures.</P><P><B>Methods</B></P><P>Male Sprague-Dawley rats, normal or with a spinal vascular clip at the level of Th9, were investigated cystometrically 1 and 4 weeks after SCI. Intravesical pressure (IVP) and intraabdominal pressure (IAP) were recorded simultaneously to evaluate true DO. During the filling phase, the event of IVP rises, defined as increments that exceeded 2 cmH<SUB>2</SUB>O from baseline, were determined as DO according to the absence of simultaneous changes in IAP.</P><P><B>Results</B></P><P>All SCI rats exhibited DO during the filling phase, which was not shown in sham rats. The frequency and pressure of DO had a tendency to decrease with time. The DO frequency of SCI rats after 4 weeks (0.9±0.2 min<SUP>-1</SUP>) was decreased compared with that after 1 week (2.1±0.4 min<SUP>-1</SUP>; P<0.05). The DO pressure of SCI rats after 4 weeks (8.4±1.9 cmH<SUB>2</SUB>O) was decreased compared with that after 1 week (11.6±2.9 cmH<SUB>2</SUB>O; P>0.05).</P><P><B>Conclusions</B></P><P>Cystometric studies in awake male SCI rats showed some significant changes in bladder function after SCI. All SCI rats exhibited DO during the filling phase, and showed different physical characteristics of DO over the course of time. The neurological basis of these time-related changes remains poorly understood, but may provide important prognostic information about long-term urological management in SCI patients.</P>

The effect of maternal exercise and altitude training on performance in hypoxic environment: a brief review

He Long Quan,Chang Keun Kim 국민체육진흥공단 한국스포츠정책과학원 2020 International Journal of Applied Sports Sciences Vol.32 No.2

Environmental and maternal exercise experienced even during the very earliest stages of life has the potential to cause developmental changes. The growing evidence demonstrated that diverse environmental stressors affect offspring in various aspects in early stage of life and can be transmitted directly or indirectly by both parental lines. The development of normobaric hypoxic environment facilities began in recent years after athletes born and trained at high altitude continued to update their records in sports competition, especially marathons and other endurance sports. Although a large number of studies have proved the effect of hypoxic training in the field of sports science and competition, the effectiveness of this training model on exercise performance/capacity and physiological variables is still controversial. Therefore, this study makes a brief review of the papers related to this scope and attempted to understand the potential mechanism of maternal exercise in hypoxic environment on exercise performance and reduction of metabolic risk factors.

수출 전 저장기간과 모의 수출에 따른 '후지' 사과의 상품성 변화

박일용,이재창,황용수 충남대학교 농업과학연구소 2002 농업과학연구 Vol.29 No.1

관행적인 수확기인 11월 6일에 수확한 '후지' 사과는 80% 이상이 밀증상을 나타내었으나 에틸렌 발생량을 기준으로 볼 때 climacteric stage에 도달하지는 않았다. 모의 수출 전 2개월 저장기간 중 밀증상은 점차 감소하였다. 저장기간 중 가용성 고형물 수준은 일정하지 않았으나 경도와 생체중 감소가 저장기간일 길수록 증가하였다. 저장기간에 따라 모의 수출과 소매과정을 경과시킨 과실의 품질은 전반적으로 낮아졌지만 무처리에 비하여 30 um 플라스틱 필름 포장, 에틸렌제거제 처리는 전반적으로 과실의 품질을 소매단계까지 우수하게 유지시키는 결과를 보였다. 반면에 왁스처리는 외관품질을 우수하게 유지시켰지만 갈변장해를 일으켜 바람직하지 않았다. 따라서 수출용 과실은 건조한 환경에서 장기간 수송되고 판매되므로 품질저하가 심하여 이를 방지하기 위해서는 플라스틱 필름과 같은 moisture barrier 및 에틸렌 제거환경으로 선적하는 것이 바람직할 것으로 판단된다. The percentage of watercore occurrence harvested on Nov. 6 (commercial harvest season) was over 80% but fruits seemed not to reach climacteric stage on the basis of ethylene evolution. The occurrence ratio and severity of watercore gradually decreased with the increase of preshipment storage for 2 months. The level of soluble solids did not show a clear tendency during preshipment storage but flesh firmness, fresh weight, and acidity gradually decreased. Although the overall quality of fruit reduced along with the increase of preshipment storage duration after exposure to retail condition at ambient temperature for 1 week, plastic film wrapping (PE 30 um) as well as plastic film wrapping plus ethylene scrubbing had a benefit on keeping freshness of fruit even after retail condition. Waxed fruit showed excellent appearance but browning disorder was developed. Results indicated that moisture barrier such as plastic film wrapping and ethylene removal have a great potential on keeping freshness of fruit during export which required long-term transport under low humidity environment.

Dynamic Analysis of Construction Safety Risk and Visual Tracking of Key Factors based on Behavior-based Safety and Building Information Modeling

Pin-Chan Lee,Junhao Wei,Hsin-I Ting,Tzu-Ping Lo,Danbing Long,Luh-Maan Chang 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.10

Construction has long been seen as a high-risk industry. Many studies conduct risk management by controlling construction risk indicators, but few studies associate risk indicators with time and space to propose long-term risk management methods. Therefore, this study proposes a dynamic analysis and visual tracking method based on behavior-based safety (BBS). This study establishes a BBS observation checklist and records workers’ unsafe behavior. The risk level of unsafe behavior is then determined by grey clustering model. When a high risk occurs, an improved grey correlation model is used to track key indicators that affect risk. In order to achieve visual risk management, this study develops semantic logic to predefine the relationship between components and space. In schedule simulation of BIM, the key indicators of BBS are transformed into an executable visual inspection between work items, components, and space through ARC. This method makes it easier for construction managers to combine time and space to manage safety risk and to adopt appropriate strategies in a timely manner to improve the efficiency of safety management.

Small activating RNA induced expression of VHL gene in renal cell carcinoma

Kang, Moo Rim,Park, Ki Hwan,Lee, Chang Woo,Lee, Myeong Youl,Han, Sang-Bae,Li, Long-Cheng,Kang, Jong Soon Elsevier 2018 The international journal of biochemistry & cell b Vol.97 No.-

<P><B>Abstract</B></P> <P>Recent studies have reported that chemically synthesized double-stranded RNAs (dsRNAs), also known as small activating RNA (saRNAs), can specifically induce gene expression by targeting promoter sequences by a mechanism termed RNA activation (RNAa). In the present study, we designed 4 candidate saRNAs targeting the Von Hippel-Lindau (VHL) gene promoter. Among these saRNAs, dsVHL-821 significantly inhibited cell growth by up-regulating VHL at both the mRNA and protein levels in renal cell carcinoma 769-P cells. Functional analysis showed that dsVHL-821 induced apoptosis by increasing p53, decreasing Bcl-xL, activating caspase 3/7 and poly-ADP-ribose polymerase in a dose-dependent manner. Chromatin immunoprecipitation analysis revealed that dsVHL-821 increased the enrichment of Ago2 and RNA polymerase II at the dsVHL-821 target site. In addition, Ago2 depletion significantly suppressed dsVHL-821-induced up-regulation of VHL gene expression and related effects. Single transfection of dsVHL-821 caused long-lasting (14 days) VHL up-regulation. Furthermore, the activation of VHL by dsVHL-821 was accompanied by an increase in dimethylation of histone 3 at lysine 4 (H3K4me2) and acetylation of histone 4 (H4ac) and a decrease in dimethylation of histone 3 at lysine 9 (H3K9me2) and lysine 27 (H3K27me2) in the dsVHL-821 target region. Taken together, these results demonstrate that dsVHL-821, a novel saRNA for VHL, induces the expression of the VHL gene by epigenetic changes, leading to inhibition of cell growth and induction of apoptosis, and suggest that targeted activation of VHL by dsVHL-821 may be explored as a novel treatment of renal cell carcinoma.</P>

Malignant transformation of ovarian mature cystic teratoma into squamous cell carcinoma: a Taiwanese Gynecologic Oncology Group (TGOG) study

An Jen Chiang,Min-Yu Chen,Chia-Sui Weng,Hao Lin,Chien-Hsing Lu,Peng-Hui Wang,Yu-Fang Huang,Ying-Cheng Chiang,Mu-Hsien Yu,Chih-Long Chang 대한부인종양학회 2017 Journal of Gynecologic Oncology Vol.28 No.5

Objective: The malignant transformation (MT) of ovarian mature cystic teratoma (MCT)to squamous cell carcinoma (SCC) is very rare. This study analyzed cases from multiplemedical centers in Taiwan to investigate the clinicopathologic characteristics, treatment, andprognostic factors of this disease and reviewed related literature. Methods: Pathological reports of 16,001 patients with primary ovarian cancer who weretreated at Taiwan medical centers from 1990 to 2011 were reviewed. In total, 52 patients withMT of MCT to SCC were identified. Results: Among all ovarian MCTs, the incidence of MT to SCC is 0.2%. The median age ofpatients was 52 years (range, 29–89 years), and the mean tumor size was 10.5 cm (range, 1–40cm). We analyzed the patients in our study and those in the literature and determined thatearly identification and complete surgical resection of the tumor are essential for long-termsurvival. In addition, adjuvant chemotherapy or concurrent chemoradiotherapy can be usedto treat this malignancy. Old age, large tumor size (≥15.0 cm), and solid components in MCTsare suitable indicators predicting the risk of MT of MCT to SCC. Conclusion: Similar to general epithelial ovarian cancers, the early detection of MT of MCTto SCC is critical to long-term survival. Therefore, older patients with a large tumor or those with a tumor containing a solid component in a clinically diagnosed MCT should beevaluated to exclude potential MT to SCC.

한국인 및 중국 한족 정신분열병 환자의 5-HT2A 수용체 유전자 -1438A/G 다형성

이장호,이광철,이승부,오용인,최영근,조아랑,정주호,장환일 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.1

Objectives : The purpose of the present study was to investigate the association between -1438A/G polymorphism of 5-HT2A receptor gene and schizophrenia in Korean and Han Chinese population. Methods : A sample of 184 Korean patients with schizophrenia and 96 Korean healthy normal controls and 96 Han Chinese patients with schizophrenia and 96 Han-Chinese healthy normal controls were genotyped for a single nucleotide polymorphism with in 5-HT2A receptor gene (promoter region, A-1438G) by Msp I Resthction Fragment Length Polymorphism (RFLP). Results : There was no difference in allelic frequencies and genotype frequencies of -1438A/G polymorphism between Korean schizophrenics and controls (p=0.13) and Han Chinese schizophrenics and controls (p=0.40). Also, -1438A/G Poly-morphism did not show ethnical difference between Korean and Han Chinese controls. The Scale for the Assessment of Negative Symptoms (SANS) scores showed no significant differences between genotypes of -1438A/G polymorphism in both of Korean and Han Chinese schizophrenics. Conclusion : These results suggest that -1438A/G polymorphism of the 5-HT2A receptor gene is not causally related to the development of schizophrenia in Korean and Han Chinese population, and there no ethnic difference between Korean and Han Chinese population.

Mechanical Error Analysis of Disk Cam Mechanisms with a Flat-Faced Follower

Chang Wen-Tung,Wu Long-Iong The Korean Society of Mechanical Engineers 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.3

By employing the concept of equivalent linkage, this paper presents an analytical method for analyzing the mechanical errors of disk cam mechanisms with a flat-faced follower. The resulting error equations do not really involve the location of the curvature center of the cam profile, and locating the curvature center of the cam profile is not essential. The resulting errors are significantly affected by the pressure angle, and the smaller pressure angle will result in the smaller mechanical error. In the worst case, owing to the joined effects of various design parameters, the accuracy of the follower motion may degrade considerably. For the oscillating follower case, all acceleration error functions have a sudden change at every beginning and at every end of the motion even though the theoretical follower displacement is cycloidal motion.

AMORPHIZATION OF ZnO NANOWIRES BY PROTON BEAM IRRADIATION

CHANG FU DEE,ISHAQ AHMAD,LONG YAN,XINGTAI ZHOU,BURHANUDDIN YEOP MAJLIS 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2011 NANO Vol.6 No.3

We report the effects of 70 keV proton (H+) irradiation on the structure of zinc oxide nanowires (ZnO NWs) for a wide range of irradiation doses at room temperature. It was found that at low dose 5 × 1015 ions/cm2 of protons, few defects were created in ZnO NWs and the defects' density was increased with an increasing proton irradiation dose. After the irradiation dose was increased to 2 × 1017 ions/cm2, the crystal structure of the ZnO NWs was almost completely damaged and the crystalline wurtzite structure of the ZnO NWs could be transformed into a disordered amorphous structure. Structural changes in the ZnO NWs upon bombardment with 70 keV protons were characterized by high resolution transmission electron microscopy (HRTEM).

Lipid accumulation mediated by adiponectin in C2C12 myogenesis

( Chang Jun Yin ),( Qin Qiang Long ),( Ting Lei ),( Xiao Dong Chen ),( Huan Long ),( Bin Feng ),( Yin Peng ),( Yan Ling Wu ),( Zai Qing Yang ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.10