http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Propylthiouracil에 의한 중증 급성간염 1예
임종주,심미령,이명수,김태현,오석규,안선호,박무림,김남호,박병현,조성구 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.2
PTU에 의한 간염의 발생은 매우 드물게 발생하는 것으로 알려져 있으나 저자들은 Graves씨 병으로 진단 받고 propylthiouracil 투여를 받고있던 환자에서 중증의 급성 간염이 발생한 1예를 경험하였기에 보고하는 바이다. Propylthiouracil is widely used to treat patients with hyperthyroidism. This drug has been associated with severe hepatotoxicity rarely. We presented the case of jaundice and profound liver dysfunction from a 23-year old woman treated with propylthiouracil for hyperthyroidism. Viral, metabolic, and autoimmune liver disease could be excluded and liver biopsy revealed a pattern of acute hepatitis. After discontinuing the drug, there was a progressive resolution of hepatic symptoms and decrease in biochemical data of the liver. Despite propylthiouracil induced hepatitis in rare case, patients receiving propylthiouracil are exposed to develope severe hepatotoxicity. Therefore it might be advisable to monitor level of the transaminase on regular base from patients receiving propylthiouracil.
Gu, Ja-Gyeong,Park, Jung-Min,Yoon, Su-Jin,Ahn, Byoung-Ki,Kang, Chang-Won,Song, Jae-Chul,Kim, Jin-Man Korean Society for Food Science of Animal Resource 2011 한국축산식품학회지 Vol.31 No.3
This study was conducted to investigate the effect of immersion treatment using lactic acid (LA) and sodium benzoate (SB) on the physicochemical quality and freshness of spent hen breast meats. A total of 135 spent hen breast meats were subjected to 9 different treatments using various concentrations of LA and/or SB in sterile DW. The 9 treatment groups were as follows: Control, sterile DW without LA or SB; T1, 1% LA; T2, 2% LA; T3, 4% LA; T4, 1% LA and 0.1% SB; T5, 2% LA and 0.1% SB; T6 2% LA and 0.2% SB; T7, 2% LA and 0.4% SB; T8, 4% LA and 0.2% SB, respectively. All groups were kept at 4oC for 15 d. The microbial counts in the control group gradually increased during storage, but those for the treated groups were significantly lower than the control or were not detected. The pH values of the control were significantly higher than those of the treated groups (p<0.05). In the color measurements, the lightness ($L^*$) and yellowness ($b^*$) values increased during storage and the redness ($a^*$) values decreased (p<0.05). The K-value and volatile basic nitrogen of the treated groups were significantly lower than those of the control group (p<0.05). Overall, the combined results of this study indicate that LA and SB could be used as favorable preservatives for spent hen breast meats to extend their shelf-life during refrigerated storage.
Ahn, Hye‐,Kyung,Kim, Byoung Chan,Jun, Seung‐,Hyun,Chang, Mun Seock,Lopez‐,Ferrer, Daniel,Smith, Richard D.,Gu, Man Bock,Lee, Sang‐,Won,Kim, Beom Soo,Kim, Jungbae Wiley Subscription Services, Inc., A Wiley Company 2010 Biotechnology and bioengineering Vol.107 No.6
<P><B>Abstract</B></P><P>An efficient protein digestion in proteomic analysis requires the stabilization of proteases such as trypsin. In the present work, trypsin was stabilized in the form of enzyme coating on electrospun polymer nanofibers (EC‐TR), which crosslinks additional trypsin molecules onto covalently attached trypsin (CA‐TR). EC‐TR showed better stability than CA‐TR in rigorous conditions, such as at high temperatures of 40 and 50°C, in the presence of organic co‐solvents, and at various pH's. For example, the half‐lives of CA‐TR and EC‐TR were 1.42 and 231 h at 40°C, respectively. The improved stability of EC‐TR can be explained by covalent linkages on the surface of trypsin molecules, which effectively inhibits the denaturation, autolysis, and leaching of trypsin. The protein digestion was performed at 40°C by using both CA‐TR and EC‐TR in digesting a model protein, enolase. EC‐TR showed better performance and stability than CA‐TR by maintaining good performance of enolase digestion under recycled uses for a period of 1 week. In the same condition, CA‐TR showed poor performance from the beginning and could not be used for digestion at all after a few usages. The enzyme coating approach is anticipated to be successfully employed not only for protein digestion in proteomic analysis but also for various other fields where the poor enzyme stability presently hampers the practical applications of enzymes. Biotechnol. Bioeng. 2010;107: 917–923. © 2010 Wiley Periodicals, Inc.</P>
Characterization of gltA::luxCDABE Fusion in Escherichia coli as a Toxicity Biosensor
Ahn, Joo-Myung,Kim, Byoung-Chan,Gu, Man-Bock The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.6
The use of gltA gene, as a new biomarker for environmental stress biomonitoring, was investigated because of its key position as the first enzyme of the tricarboxylic acid (TCA) cycle. A recombinant bioluminescent Escherichia coli strain, EBJM2, was constructed using a plasmid carrying the citrate synthase (gltA) promoter transcribing the Photorhabdus luminescens IuxCDABE genes (gltA::luxCDABE). The responses from this strain were studied with five different classes of toxicants: DNA damage chemicals, phenolics, oxidative-stress chemicals, PAHs, and organic solvents. EBJM2 responded strongly to DNA damage chemicals, such as mitomycin C (MMC) and methyl-nitro-nitrosoguanidine (MNNG) and nalidixic acid with the strongest responses. In contrast, tests with several compounds from the other four classes of toxicants gave no significant response. Therefore, EBJM2 was found to be sensitive to DNA damage chemicals.
PCR-Based Sensitive Detection and Identification of Xanthomonas oryzae pv. oryzae
Lee, Byoung-Moo,Park, Young-Jin,Park, Dong-Suk,Kim, Jeong-Gu,Kang, Hee-Wan,Noh, Tae-Hwan,Lee, Gil-Bok,Ahn, Joung-Kuk 한국미생물 · 생명공학회 2004 한국미생물·생명공학회지 Vol.32 No.3
본 연구는 벼의 세균병 중 치명적인 흰잎마름병을 유발하는 Xanthomonas oryzae pv. oryzae를 검출할 수 있는 프라이머를 개발하기 위해 실시하였다. X. o. pv. oryzae str. KACC10331의 hpaA유전자 염기서열로부터 흰잎마름병만을 특이적으로 검출할 수 있는 프라이머를 제작하여 중합효소연쇄반응에 사용하였다. 개발된 특이 프라이머는 X. o. pv. oryzae str. KACC10331과 X. campestris pv. vesicatoria, X. campestris pv. campestris, X. axonopodis pv. citri 그리고 X. axonopodis pv. glycines의 phaA유전자 염기서열의 상동성을 비교하여, 그 중 X. o. pv. oryzae만이 가지는 특이적인 부분을 바탕으로 각각 20-mer인 XOF와 XOR를 제작하였다. 제작된 프라이머를 이용하여 중합효소 연쇄반응을 실시한 결과 반응 후 생성된 단편의 크기는 534-bp였다. 반응 후 생성된 단편은 Southern hybridization을 통하여 Xanthomonas 균주들의 hpaA유전자 존재 여부 및 그 상동성을 비교분석하기 위해 사용하였다. 또한 제작된 프라이머를 이용하여 흰잎마름병에 감염된 벼 잎에서의 검출 여부를 확인하였고 X. o. pv. oryzae의 순수 균주 배양액을 중합효소연쇄반응에 이용하여 검출한계를 검정하였다. 본 연구에서 제작된 프라이머를 사용한 중합효소연쇄 반응 방법은 X. o. pv. oryzae의 검출 뿐만 아니라 흰잎마름병의 발생 예찰에 매우 유용할 것으로 판단 되었다. A new primer set was developed for the detection and identification of Xanthomonas oryzae pv. oryzae, the bacterial leaf blight (BLB) pathogen in rice plant. The nucleotide sequence of hpaA gene was determined from X. o. pv. oryzae str. KACC10331, and the sequence information was used to design primers for the application of the polymerase chain reaction (PCR). The nucleotide sequence of hpaA from X. o. pv. oryzae str. KACC 10331 was aligned with those of X. campestris pv. vesicatoria, X. campestris pv. campestris, X. axonopodis pv. citri, and X. axonopodis pv. glycines. Based on these results, a primer set(XOF and XOR) was designed for the specific detection of hpaA in X. o. pv. oryzae. The length of PCR products amplified using the primer set was 534-bp. The PCR product was detected from only X. o. pv. oryzae among other Xanthomonas strains and reference bacteria. This product was used to confirm the conservation of hpaA among Xanthomonas strains by Southern-blotting. Furthermore, PCR amplification with XOF and XOR was used to detect the pathogen in an artificially infected leaf. The sensitivity of PCR detection in the pure culture suspension was also determined. This PCR-based detection methods will be a useful method for the detection and identification of X. o. pv. oryzae as well as disease forecasting.
Highly stable trypsin-aggregate coatings on polymer nanofibers for repeated protein digestion
Kim, Byoung Chan,Lopez-Ferrer, Daniel,Lee, Sang-Mok,Ahn, Hye-Kyung,Nair, Sujith,Kim, Seong H.,Kim, Beom Soo,Petritis, Konstantinos,Camp, David G.,Grate, Jay W.,Smith, Richard D.,Koo, Yoon-Mo,Gu, Man B WILEY-VCH Verlag 2009 Proteomics Vol.9 No.7
<P>A stable and robust trypsin-based biocatalytic system was developed and demonstrated for proteomic applications. The system utilizes polymer nanofibers coated with trypsin aggregates for immobilized protease digestions. After covalently attaching an initial layer of trypsin to the polymer nanofibers, highly concentrated trypsin molecules are crosslinked to the layered trypsin by way of a glutaraldehyde treatment. This process produced a 300-fold increase in trypsin activity compared with a conventional method for covalent trypsin immobilization, and proved to be robust in that it still maintained a high level of activity after a year of repeated recycling. This highly stable form of immobilized trypsin was resistant to autolysis, enabling repeated digestions of BSA over 40 days and successful peptide identification by LC-MS/MS. This active and stable form of immobilized trypsin was successfully employed in the digestion of yeast proteome extract with high reproducibility and within shorter time than conventional protein digestion using solution phase trypsin. Finally, the immobilized trypsin was resistant to proteolysis when exposed to other enzymes (i.e., chymotrypsin), which makes it suitable for use in “real-world” proteomic applications. Overall, the biocatalytic nanofibers with trypsin aggregate coatings proved to be an effective approach for repeated and automated protein digestion in proteomic analyses.</P>
비 대면실명확인 시스템 구축 이후 금융기관의 효과적인 마케팅 시스템 구축 방안 연구
안병용(Ahn Byoung Yong),이구연(Lee Gu Yeon),조희준(Jo Hee Jun) 한국IT서비스학회 2015 한국IT서비스학회 학술대회 논문집 Vol.2015 No.추계
전통적인 금융기관의 영업형태는 고객이 지점을 방문하여 대면거래 형태로 대부분 이루어졌으나, 2000년 초부터 인터넷뱅킹 등 금융거래의 비대면화가 시작된 이래로 관련 IT기술발전과 더불어 금융기관의 비대면 채널의 비약적인 발전으로 금융기관을 이용하는 고객이 영업점 방문 없이 대부분의 금융거래가 가능 해짐에 따라 금융기관은 새로운 온라인 마케팅을 위한 시스템 구축이 절실하게 필요하게 될 것으로 예견된다. 이에, 대면 마케팅 역할을 대신하는 새로운 형태의 마케팅 채널 시스템 구축 및 활성화를 본 연구의 목적으로 하고자 한다.
Shin, Gu-Choul,Na, Byoung-Kuk,Lee, Joo-Yeon,Ahn, Jung-Bae,Park, Jong-Won,Lee, Jin-Soo,Kim, Jee-Hee,Kim, Woo-Joo,Kang, Chun The Korean Society for Microbiology 2003 Journal of Bacteriology and Virology Vol.33 No.4
Although Korean influenza virus isolates have been genetically associated with the vaccine strains of the corresponding year, influenza B viruses have prevailed almost every year in Korea during the past decades. We have 'analyzed the genetic characteristics and evolutionary patterns of the haemagglutinin (HA) 1 domains of influenza B viruses isolated during 1988-1999 using direct RT-PCR and sequencing. Phylogenetic analysis of influenza B viruses isolated in Korea indicated that antigenically and genetically distinguishable strains of the lineage II and lineage III variants had been cocirculated. Variants prevailed in early 1990s are represented in 1996/97 and 1998/99 and some different variants have been co circulated geographically and prevailed concurrently in Korea. All HA1s of Korean isolates have amino acid substitutions mainly in the region between position 124 and 310, which was previously proposed an immunodominant region. Insertion-deletion patterns of the HA gene revealed that Korean influenza B viruses were evolved from Lee40 with different manner between lineage II and III viruses. Lineage III viruses were also divided into two groups as conserved group and inserted group, in relation to Lee40. But lineage II viruses had evolved with directional pattern. Antigenic index proposed that influenza B isolates prevailed since 1996/97 seasons might had emerged from the antigenic variants of a Seo1697-like virus and that new variants might appear from the lineage II viruses resulting in persistent prevalence in Korea.