DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

도시공원의 이용후 평가에 기초한 공원의 개발지표 설정 : 부산광역시 암남공원을 사례로 Case Study of Amnam Park in Busan City

박승범,김승환,남정칠,강영조,이기철,김석규 동아대학교 환경문제연구소 1999 硏究報告 Vol.22 No.2

The purpose of this study is to investigate the prime factors that affect to user postoccupancy evaluation of activities and facilities in urban parks, to classify those factors, to find out factors about them, and then to suggest important indicators in the urban park planning, design, and development. The survey research was done using the self-administered questionnaire method by 435 peoples in the Busan City who participated in recreational activities in the Amnam Park. The Analysis of the data primarily consists of two phase: The first analysis was dealt an exploratory factor analysis which identified major factors involved in user postoccupancy evaluation with reacreational activities and facilities in Amnam Park by the SPSS(Ver.6.0) program and the second analysis was used to investigate the factors affected to user postoccupancy evaluation in the urban park by the Regression Analysis. According to the factor analysis, user postoccupancy evaluation of facilities in urban parks concerning the 20 variables, 5 prime factors by the staistical values were considered. The factor analysis showed that user postoccupancy evaluation of activities in urban parks concerning the 11 variables, 3 prime factors by the staistical values were analysed. Postoccupancy evaluation in the urban park by the Regression Analysis suggested that the two varlables as 'the directions of park development' and 'companies with the personal specific characteristics' are significant in addition to the some variables presented in user postoccupancy evaluation with reacreational activities and facilities in Amnam Park by the factor analysis. Furthomore the analysis models were identified as having significant effects on the factor and regression analysis. Overall fits of both models were very good. These significant relationships indicate important factors and variables that should be considered in planning, design, and development of the urban parks. Therefore, development direction of the h a m park has to be analyzed as significant factors concerning user postoccupancy evaluation in a systematic way, recognized as important variables for planning process. The urban park has to be fully designed and established in the planning process not by the decision-maker's subjective evaluation but by user postoccupancy evaluation.

저전력 주차 안내 시스템 설계

박주용(Ju-Yong Park),임형규(Hyeong-giu Lim) 한국엔터테인먼트산업학회 2011 한국엔터테인먼트산업학회논문지 Vol.5 No.1

The parking guidance system can increase driver’s convenience with detailed parking information service in the parking lot. At the same time, parking guidance system consumes non-negligible electrical energy with large amount of sensors, displays and control modules. With the increase in the demand for green and sustainable building design all over the world, it becomes a meaningful issue for parking guidance system to reduce operating power. This paper presents the preliminary design and estimated results of a parking guidance system which is optimized to reduce the power consumption mainly on detectors and displays. The system design is based on pre-developed wireless parking detectors, Park Tile and Park Disk. The system has a number of parking space detectors, vehicle count detectors, information displays, guidance terminals and other control units. We have performed system architecture design, communication network design, parking information service scenario planning, battery life regulation and at last operating power estimation. The estimated operating power was 0.4 watt per parking-slot, which is 17% of traditional systems. The estimated annual maintain cost was 43% of traditional systems.

상용차 기반에 대한 주차보조시스템 사용자 감성 평가

전재석(Jaeseok Jeon),박현배(Hyunbae Park),문희석(Heeseok Moon),곽수진(Sujin Kwag) 한국자동차공학회 2013 한국자동차공학회 부문종합 학술대회 Vol.2013 No.5

In this paper, we described about evaluation of human sensibility using park assist system of the commercial vehicle. The perpendicular parking and parallel parking have been carried out as a user test and subjects are classified as beginning drivers, drivers with less than 1 year experience and over 3 years experience. As a result, parking duration of beginning drivers and drivers with less than 1 year experience who use automatic parking assistance system is similar to drivers with over 3 years experience. However error occurred according to sensor position and distance between vehicle and parking space. And also it occurred that the vehicle leaves from parking line or does not recognize the parking space. Therefore, if we improve its completeness by compensating problems that came up from this evaluation and distribute the automatic parking assistance system, it is expected that the park assistance system is to help driver providing convenience of parking.

서구 공원 녹지 배치 계획 이론의 초기 발전 과정과 특성

박구원 ( Koo Won Park ) 한국녹지환경디자인학회 2006 녹지환경학회지 Vol.2 No.2

In America and Europe from the latter part of the 19th century to the beginning of the 20th century, the planning ideologies of park system and greenbelts were made and these planning ideologies are called Green Network System. They are different in terms of the purpose, type and method, but they have common characteristics of encouraging order and amenity in cities based on the consecutive connection of green space, and of coping with the problems happening in cities. Just as the park system was made, centering around new America and greenbelts were developed to meet with the problems happening in large cities, the structural arrangement and the roles of greens should be different. However, this connection system of greens was oriented in the circumstances of America and Europe at that time so it has not been confirmed what sizes of cities it would be useful for. On the other hand, separately from the Green Network System, the theory of park arrangement is being developed. It intends to cope with deterioration of the living environment caused by overcrowded cities and with increase in the demands of public parks. It is based on the neighborhood unit and the science of human settlement. In addition, the theory of park arrangement is based on the effects of greens in use, rather than, in decoration so it encourages dispersion and hierarchical system of parks, having the human settlement as base. Consequently, the theory of park arrangement is influenced by the characteristics and the type of households, and it has a required area and planning unit. However, this theory has the right for walking and human scale as the basic unit so for the large parks that are beyond the human scale, the planning criteria are not clear. The theory of park arrangement, which is based on the neighborhood unit, may be useful for the cities with tens of thousands of residents, but it is partially applied for the cities with 500,000~1,000,000 residents. And further, in organizing the green system for the whole city, the theory of park arrangement can be ignored. As seen in the above, the existing arrangement theory has some problems: it is just for partial application; and it is not suitable for the size and the growing speed of cities. The greens in cities should be operated as the comprehensive system, but greenbelts and park arrangement have been studied separately so far. That`s why it is necessary to review again the existing arrangement theory.

공원범죄의 피해방지를 위한 합리적인 방안

박찬걸(Park, Chan-Keol) 한국피해자학회 2012 被害者學硏究 Vol.20 No.2

Before constructing parks, it's necessary to formulate plans in the direction of preventing crimes in advance, and follow-up management must be done after constructing the parks completely. If citizens are reluctant to use the parks because public safety is not guaranteed, the reason for being disappears. If the parks do not protect citizens from crimes, it would rather not exist than do harm to them. Rather, it is good policy. In this respect, the current system must be improved, which has no manual for crime prevention being confined to policy of increasing the number of parks. Through the government's expressing its firm will to secure public safety of parks by enacting clear provisions so that the original good will is not distorted to make resting places for citizens, a recognition that illegal acts which make others anxious are punished necessarily must be instilled into park users while taking a sound rest is protected thoroughly. In addition, the police should do the patrols actively in order to prevent crimes in the parks and try to increase street lamps and security CCTV installation in collaboration with the agencies concerned including gu offices. However, it can be said the most important thing to prevent damage of the crimes in the parks is the very civic consciousness. We should keep it in mind that along with continuous efforts by the police and public organizations, a sense of ownership that 'I am the owner of the park' and mature civic consciousness to ‘care for the park like my house’ will make the parks safe without crimes.

박두진 시인의 유년기와 시력(詩歷)의 상관성 연구

박홍배(Park, Hong-Bae) 한국시학회 2018 한국시학연구 Vol.- No.54

본 연구는 박두진의 유년시절의 의식이 박두진의 성장기는 물론이고 청년기와 장년기, 나아가 노년기에 이르기까지 영향을 미친다는 관점을 보이고 있다. 박두진의 특수한 경험에 해당되는 유년기의 가난은 박두진을 좌절시키기보다는 오히려 박두진의 일생에 걸쳐 박두진을 곧고 강한 인물이 되도록 할 수 있었던 요인이 되었던 것이다. 물론 유년기의 불우했던 환경이 모든 이에게 긍정적인 방향으로 귀결되지는 않았을 것이나 박두진은 이러한 조건에 굴복한 것이 아니라 이를 딛고 자신의 인생을 개척했음을 알 수 있다. 이는 박두진의 강직하고도 자의식 강한 성품을 말해주는바, 본고를 통해 박두진의 유년기의 정신적 풍경을 살펴봄으로써 그의 어떠한 요인이 그의 삶을 바르고 곧은 길로 이끌었는지 확인하고자 하였다. 지독한 가난으로 잦은 이사를 다니면서 동네 아이들로부터 심한 따돌림으로 고통 받아야 했던 박두진에게 산과의 인연은 절대적인 요인으로 다가왔다. 가난의 고통과 텃세로 인해 죽음을 생각할 만큼 열등감에 시달렸던 박두진에게 산은 놀이 공간이자 안식처였고 구원의 지대였다. 또한 산은 박두진에게 하나의 공동체였고 삶의 터전이었다. 박두진은 온갖 생명이 생기가 넘쳐나는 산을 통해 자유와 평등과 사랑으로 공동체를 이루고 살아가는 모습을 보게 되었고 생의 의욕을 다지게 된다. 산을 오르내리면서 얻게 되었던 이러한 체험은 소년 박두진을 좌절로부터 일으켜 세움으로써 그의 이상과 의지와 꿈을 형성하게 한 계기가 되었다. 이는 박두진이 에릭슨이 말한 바 유아 남성성의 단계를 슬기롭게 겪음으로써 ‘유능함’의 삶의 과정을 거치게 되었음을 말해준다. 유년기 산에서의 체험에 힘입어 박두진은 인생의 매 시기 강건하고 올곧은 선택을 할 수 있었고 이는 박두진의 시력의 세 단계로 나타나게 되었다. 그것은 첫째 산에서의 행복했던 체험에 바탕을 둔 초기 자연시와 관련되며 둘째 산에서 발견한 공동체의 모습에 의지하여 중기에 보여주었던 사회참여시와 관련되고, 셋째 산에서 얻게 된 이상적 공간에 대한 감각에 따른 후기의 신앙시와 관련된다. 산에서 외로움과 고통으로부터 벗어날 수 있었던 박두진에게 산은 안식의 공간이었을 뿐만 아니라 이상적인 공동체였으며 바른 삶을 살 수 있도록 하는 안내자였던 것이다. 이는 산에서 겪었던 유년기의 체험이 그의 시력에 결정적인 영향력으로 작용했음을 말해준다. 즉 소년 박두진은 산을 오르내리면서 많은 생각을 하게 되었고 이때 다져진 그의 굳은 의지는 박두진의 가슴에 화석처럼 쌓여 일생 동안 길잡이가 되었던 것이다. 박두진의 초기, 중기, 후기의 시들이 모두 변함없이 일관되게 강직하고도 완전한 형태로 이루어질 수 있었던 것도 이러한 사정에 기인한다. This study shows that Park’s childhood consciousness influences Park’s growing years, youth, and even the first years of life. The childhood poverty, which is equivalent to Park Doo-Jin’s special experience, could have made Park Doo-Jin a straight and strong character over her lifetime rather than let Park Doo-Jin be frustrated. Of course, the poor childhood environment may not have resulted in a positive one for everyone, but Park Doo-Jin overcame these conditions to learn about it. It shows Park’s strong and self-conscious character, and reveals what factors led Park in his life to the mental landscape. For Park Doo-Jin, who had to suffer from severe bullying by local children after moving around a lot due to extreme poverty, the bond with the mountain has become an essential factor. For Park Doo-Jin, who suffered from poverty and inferiority complex that made him think about his death, the mountain was a playground, a haven, and a land of salvation. The mountain was also a community for Park Doo-Jin and a place to live. Park Doo-Jin saw all kinds of lives forming a community of freedom, equality and love through the lively mountain, and he became more passionate about his life. The experience, which was gained from climbing the mountain, helped raise the boy Park Doo-Jin from frustration, forming his ideals, will, and dreams. It tells us that Park Doo-Jin has gone through what Erickson calls the “capable” lifestyle process by going through the smart phase of infant manhood. Thanks to his experiences in the mountain, he was able to make strong, straight choices during each phase of his life, which appeared to be three stages of Park’s vision. First, it is related to the early natural poetry based on the mountain experience, second, it is associated with the social participation in the latter phase of the year, depending on the appearance of the community found. Third, it is related with the latter faithful poetry. For Park Doo-Jin, who was able to escape from loneliness and suffering in the mountain, it was not only a place of rest, but also an ideal community and a guide to a just life. It suggests that his childhood experiences in the mountain served as a decisive factor in his vision. The boy, Park Doo-Jin, came up with many thoughts as he traveled around the mountain, and his strong will was wrapped up in Park’s chest like a fossil, making him a guide throughout his life. It is also due to these circumstances that Park Doo-Jin’s early, mid-and-after and later-the-day poems could all be performed consistently and in a complete form.

변화와 변용으로 본 근대기 서울 남산의 공원

박희성,Park, Hee-Soung 한국조경학회 2015 韓國造景學會誌 Vol.43 No.4

Unlike other mountains in Korea, Seoul Namsan(南山), which is one of the landmarks that represent the country's capital city, is perceived as a city park. This article aims to study the process that Namsan became a park and the transformation of its place in Korean Emperor and Japanese colonial period. The serial changes in Namsan, in early modern era, mean that is associated with the colonial urbanization and the rule of space by Japan. The stages of Waeseongdae Park(倭城臺公園), Gyeongseong Park(京城公園) and Hanyang Park(漢陽公園) under the leadership of Japan, due to the extension of Japan's power, Namsan has became the park. Here, the park has become a strategic tool of other's occupying Hanseongbu(漢城府), a capital city. The process that Namsan became a park dose not mean making the space for recreation and rest, but is an excuse for using the land. Since then, Namsan's parks barely fulfilled its original function as park as it was transformed into a shrine, Gyeongseong Jinjya(京城神社), for Japanese warriors or was incurred upon by Joseon Singung(朝鮮神宮), which was established as a facility to govern Korea, sometimes is gradually and sometimes is mercilessly. The fact, transplantation of Japanese culture and replacement as ruler space, is another aspect of occupying and govern place. In other words, while the process that Namsan became a park is the way of establishing Japanese force, the transformation of its place show a colonial rule as an aspect of space. Meanwhile, in spite of transformation to shrine, Namsan became accepted as a park for a long time, because of the forest of Namsan. Japan managed forest as a sacred place. It is also a result of the Japanese rule of space.

공공개발지구 내 주차장용지에 주차전용건축물이 집합건물일 경우 부대시설 수분양자의 주차장 지분배분에 관한 연구

박창률(Park, Chang-Yul),윤정득(Yoon, Jeong-Duck) 대한국토·도시계획학회 2020 국토계획 Vol.55 No.7

In the past, the implementers (public enterprises or local public corporations) of projects in public development districts sold parking lot land to local governments for free or at cost. However, after the parking-lot law was amended in 1999, the land was sold to the private sector under a general competitive bidding method. As a result, the provision of parking lot land in public development project districts did not do more to ameliorate the parking shortage than when it was supplied to local governments. While there are a variety of reasons for this, the most significant cause is that the development of parking lots involved parking-only buildings, but in most cases collective building were built. In general commercial land, semi-residential, or residential areas, the requirements for establishing an aggregated building are clearly defined in the Collective Building Act, but the requirements for parking areas are applied differently. That is, the law was often misinterpreted. In the case of parking-only buildings for parking lots, it is necessary to establish a system of laws to better ensure the securing of parking lots from the perspective of pre-sale consumers. This study argues that the conditions of collective buildings must be met for parking-only buildings. Local governments stipulate the allowable range of additional facilities for parking lots by ordinance, but if the construction of parking-only buildings involves a collective building, it cannot be sold under the same concept as the general commercial building. This study concludes that revision of the Enforcement Rules of the Parking Lot Law is requires to protect these rights by securing the parking lot shares of those receiving pre-sale facilities.

의열단원 박재혁(朴載赫)의 생애와 부산경찰서 투탄(投彈)

박철규 ( Park Chul-kyu ) 부산광역시사편찬위원회 2019 항도부산 Vol.37 No.-

Jae-hyuk Park was born as the only son in 183 Beomil-dong on May 17, 1895. At the age of 15, his father passed away and he lived with his mother and sister. On March 22, 1911, he graduated from Busanjin Primary School, and on March 22, 1915, he was the fourth graduate of Busan Public Commercial School. While attending Busan Public Commercial School, Jae-hyuk Park secretly reproduced and distributed 『Dongguk History』 with Cheon-taek Choi, Byeong-tae Kim and Hong-gyu Park. In his third grade, he formed Gusedan(救世團) with Cheon-taek Choi, Byeong-tae Kim, Hong-gyu Park, and Jae-young Oh, and issued a noticed to promote national identity in Busan and the whole region of Gyeongsangnam-do Province. Jae-hyuk Park left Busan in June 1917 to Shanghai and Singapore for more than three years to work in the trade industry. At the same time, he was seeking ways to contribute to the independence movement while interacting with his companions. He then decided to devote himself to the independence movement after the March 1 Independence Movement. Busan Police Station was targeted as the attack after the March 1 Independence Movement when Euiyeoldan had been organized by declaring the change of direction to the existing independence movement, and it was when everyone involved was arrested upon failure of the first national full-scale attack plan in June 1920. As a result, Euiyeoldan needed to achieve results and reverse the situation in a short time. Busan was also a gateway from Japan to Joseon and was developed as the typical colonial city, and had its symbolism as it was called as the second Osaka. In preparation for the rebellion, Jae-hyuk Park obtained information that Hashimoto, the Busan police chief, liked ancient books and disguised himself as the Chinese ancient book dealer by taking advantage of his experience in trade business. At about 2:30p.m. on September 14, 1920, he entered the Busan Police Station pretending to be on duty, approached chief Hashimoto from the right side and threw a bomb after declaring himself as a member of Euiyeoldan when the chief turned his body towards him. At that time, chief Hashimoto was slightly injured. Jae-hyuk Park was arrested at the scene after failing to escape due to his injury. Jae-hyuk Park was consistently charged with 'attempted murder' from the first trial of the Busan District Court to the third trial of the Gyeongseong High Court on March 31, 1921. Jae-hyuk Park was sentenced to life imprisonment at the Busan District Court. However, the appeal by the prosecution annulled the original decision at the trial at the Daegu High Court, and Jae-hyuk Park was sentenced to death. Eventually, Jae-hyuk Park was sentenced to death as his final judgment on March 31, 1921 after the third trial of the Gyeongseong High Court. Jae-hyuk Park suffered from lung diseases due to tortures after arrest and injuries from the bomb, but his spirit was never thwarted. He died in Daegu prison at 11:20a.m. on May 11, 1921 before the execution.