녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

회분식 반응기에서의 공정변수 변화에 의한 침강성 탄산칼슘 제조

신보철,한상오,김주호,송지훈,송근호,이광래 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.B

For the preparation of calcium carbonate particles from aqueous Ca(OH)2 slurry, carbonation reaction of aqueous Ca(OH)2 slurry was carried out by batch method the CO2 into reactor filled with aqueous slurry of Ca(OH)2. The concentration of Ca(OH)2 varies from 1.00 to 7.00wt%, reactor temperature at 20 and 40℃, and reactor pressure from atmospheric pressure to 6.0kgf/cm2. Crystal structure of calcium carbonate was of calcite, the particle size were about 0.05 ∼2.0㎛, and the particle shape was cubic and spindle. When reactor temperature was higher, particle size of calcium carbonate was bigger and particle share was varied, but reaction rate was increased. When reactor pressure was higher, particle size of calcium carbonate was smaller, particle shape was cubic, and reaction ramie was increased.

분사법에 의한 탄산칼슘 제조

신보철,한상오,김주호,송지훈,송근호,이광래 강원대학교 석재복합신소재제품연구센터 2001 석재연 논문집 Vol.6 No.-

본 연구는 탄산가스화법에 의한 탄산칼슘 입자 제조 공정에 있어서, 탄산가스로 채워진 반응기에 수산화칼슘 슬러리를 분사하는 방법으로 탄산칼슘 입자를 제조하였다. 수산화 칼슘의 농도(0.25~1.0wt%), 분사압력(0.5kgf /㎠~1.5kgf /㎠) 및 반응기 온도(20℃, 28℃)의 변화에 따른 생성된 탄산칼슘을 관찰하였다. 수산화 칼슘의 농도가 비교적 높으면(0.75wt%, 1.0wt%), 생성물의 pH와 전도도는 높게 나타났다. 분사압력에 의한 탄산칼슘 제조의 변화는 분사압력이 높아질수록(1.5kgf /㎠), pH와 전도도는 높게 측정되었다. 그러나, 수산화 칼슘의 농도와 분사압력의 변화가 탄산칼슘의 입자크기, 형태, 결정구조에 영향을 미치지는 않았다. 반응기의 온도가 28℃에서 생성된 탄산칼슘은 1.0㎛이하의 칼사이트 상 입자가 제조되었으며, 20℃에서 생성된 탄산칼슘은 0.5㎛이하의 칼사이트 상 입자가 제조되었다. 반응기의 온도가 낮아질수록 탄산칼슘 입자의 크기가 작아짐을 알 수 있었다. For the preparation of calcium carbonate particles from aqueous Ca(OH)2 slurry, carbonation reaction of aqueous Ca(OH)2 slurry was carried out by spouting the aqueous slurry of Ca(OH)2 into reactor filled with CO2 gas. The concentration of Ca(OH)2 varies from 0.25wt% to 1.0wt%, nozzle pressure from 0.5kgf /㎠ to 1.5kgf /㎠, and reactor temperature were set at 20℃ and 28℃. With relatively high concentration of 0.75wt% and 1.0wt% of Ca(OH)2, pH and conductivity of product-stream were observed high value. High pH and conductivity were observed at high nozzle pressure of 1.5kgf /㎠. The concentration of Ca(OH)2 and nozzle pressure, however, did not change the particle size and shape of calcium carbonate synthesized by carbonation reaction. The reaction temperature affected particle size. At reactor temperatures of 28℃ and 20℃, the particle sizes were about 1.0㎛ and 0.5㎛, respectively. Crystal structure of calcium calcium carbonate was of calcite. When reactor temperature was lower, the size of calcium carbonate particle was smaller.

非公式 行政作用의 考察

愼保晟 水原大學校 社會科學硏究所 1990 社會科學論集 Vol.2 No.-

흉부 위국소화 감각 수준으로 발현된 경부 추간판 탈출증

이보람,조동신,윤신구,조상걸,안무영,성기범 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5

2.methyl - 1.3dioxolane-water 계의 등온 기액평형

신동우,이보성,박소진 忠南大學校 産業技術硏究所 1987 산업기술연구논문집 Vol.2 No.2

In this work, we investigated the vapor-liquid equilibrium data of the system 2.methyl-1.3dioxolane - water, in order to provide elementary data for separation of 2.methyl-1.3dioxolane and water. Experiment was carried out at 30℃ and 35℃ isotherm by new head space analysis method, which was developed by ourselves. This binary system has the minimum azeotrope in both isothermal condition and has relatively large value of activity coefficient. It indicate that this system is nearly nonideal. Some model parameters, such as Wilson, NRTL, etc., were evaluated. With these parameters, each isothermal data was recalculated. We compared experimental data with recalculated data. By NRTL parameter fit experimental data best.

또 하나의 사회 : 광고에 표현된 한국적 vs 서구적 인간관계

성영신,허태균,김보경,박정선 한국방송광고공사 2006 광고연구 Vol.0 No.73

본 연구에서는 광고 비주얼에 표현된 인간관계의 문화적 차이에 따라 소비자의 심리반응이 어떻게 다른지 비교해보았다. 한국적(서구적) 인간관계를 부모 - 자녀, 온가족, 부부, 연인의 네 가지 관계유형으로 나누어 이를 묘사한 인쇄 광고물을 제작한 후, 소비자의 심리반응을 fMRI(기능성 자기 공명 영상) 기법과 설문조사 방법으로 측정하였다. 소비자는 무의식적인 fMRI 반응과 의식적인 반응 모두에서 한국적 광고보다는 서구적 광고에서 더 좋은 반응을 보였다. 특히, 광고효과의 경우 연인광고를 제외한 다른 인간관계 광고들 모두에서 서구적 광고가 더 효과적인 것으로 나타났다. 이렇게 전반적으로 서구적 인간관계 광고가 강한 신경 반응과 긍정적 광고효과를 수반하는 것은 한국인들이 상당히 서구적인 가치관을 가지고 있으며 이를 지향한다는 최근 연구결과와 일치한다. 끝으로, 본 연구결과가 갖는 크리에이티브 측면의 시사점을 제안하였다. 유형별 차이로는 연인이나 부부 광고는 무의식적 반응이나 감정반응에서 한국적인 광고가 보다 긍정적인 결과를 보이는 반면, 가족이 등장하는 광고에서는 서구적 광고가 더 긍정적인것으로 나타났는데, 이는 실험참가자가 인간관계 유형에 따라 다른 문화적 인식과 지향점을 갖으며, 이것이 광고반응에 영향을 미치고 있음을 보여준다. The purpose of the present research was to compare the psychological effects of the Korean human relations advertise(KHRA) to those of the Western human relations advertise(WHRA). The consumers’ physiological and conscious responses to the ads including visual images of Korean(vs. Western) four types of relationship - parentschildren, multi-generation family, married couple, and romantic couple - were measured by fMRI(functional Magnetic Resonance Imaging) and survey. The results revealed that consumers tend to respond more strongly and better to the WHRAs than the KHRAs. Especially, in terms of advertising impacts, the WHRAs were more effective than the KHRAs across the ads of all the three human relations except the ads of romantic couples. These results are consistent with the recent research findings suggesting the Westernizing sifts of Korean cultural values. Finally, they were discussed in the view of the implications of advertising creative tips.

土地財産權의 保障과 規制에 관한 硏究 : 土地公槪念論을 中心으로 mainly on the subject of "public conception of the land"

愼保晟 水原大學校 社會科學硏究所 1989 社會科學論集 Vol.1 No.-

1) Landproperty schould be handled differently due to its own nature. Land can not be produced, increased, moved nor decreased. In addition to that its owner's way of use influences neighbours extraordinary. Also, land is becoming more and more scare natural sources. The situation is getting worse owing to industrialization and urbanization. Originally, Korea is a small and narrow peninsular, 70% of which are mountains. 2) At present, the phrase "public conception of the land" is popular in Korea. Government has prepared relevent bills as for limitation of housing land owning, restriction of developmentsgain and etc. That kind of work is accelerated by land speculation and it's result of land monopoly. Also disparity between rich and poor, largely generated by land ownership and land speculation, is not tolerated in Korean Constitutional Law. Korea is not only democratic but also social, welfare-state. Government must endeavor to establish social justice among which regulation of execcive land speculation and owning. 3) Due to land speculation and it's monopoly the necessity of regulation on landproperty and land-trade have been urged already. What are mattered mainly in legal side is how to harmonize between guarantee of property and it's regulation. This report aims to solute such legal problem. Fourtunately, our Korean Constitutional Law enables that task as it contains relevant clauses.

주요 우울증을 동반한 신경성 식욕과항진증 1례

신석호,권용실,김보연,이정태,이성필 한국의학사 1992 診斷과治療 Vol.12 No.7

HBsAg 및 류마토이드 인자 공존예의 분석 : Frequency and Clinical Significance

신보문,조종래,백인기,박성섭 대한임상병리학회 1996 대한임상병리학회지 Vol.16 No.5