Genetic Variation of SCN5A in Korean Patients with Sick Sinus Syndrome

Young-Soo Lee,Michael A Olaopa,정병천,이상희,신동구,박형섭,조용근,한상미,이명훈,김윤년 대한심장학회 2016 Korean Circulation Journal Vol.46 No.1

Background and Objectives: Due to recent studies that have shown an association between the genetic variation of SCN5A and sick sinus syndrome (SSS), we sought to determine if a similar correlation existed in Korean patients with SSS. Subjects and Methods: We enrolled 30 patients with SSS who showed a sinus pause (longer than 3.0 s) in Holter monitoring, in addition to 80 controls. All exons including the putative splicing sites of the SCN5A gene were amplified by polymerase chain reaction and sequenced either directly or following subcloning. Wild-type and single nucleotide polymorphisms were expressed in human embryonic kidney cells, and the peak sodium current (INa) was analyzed using the whole-cell patch-clamp technique. Results: A total of 9 genetic variations were identified: 7 variations (G87A-A29A, IVS9-3C>A, A1673G-H558R, G3823A-D1275N, T5457C-D1819D, T5963G-L1988R, and C5129T-S1710L) had been previously reported, and 2 variants (A3075T-E1025D and T4847A-F1616Y) were novel; the potential structural effects of F1616Y were analyzed in a three-dimensional model of the SCN5A domain. Patch-clamp studies at room temperature demonstrated that the peak INa was significantly increased by 140% in HEK cells transfected with F1616Y comparedwith wild-type (-335.13 pA/pF±24.04, n=8 vs. -139.95 pA/pF±23.76, n=7, respectively). Furthermore, the voltage dependency of theactivation and steady-state inactivation of F1616Y were leftward-shifted compared with wild-type (Vh activation=-55.36 mv±0.22, n=8 vs. Vh activation=-44.21 mV±0.17, n=7; respectively; Vh inactivation=-104.47 mV±0.21, n=7 vs. Vh inactivation=-84.89 mV±0.09, n=12, respectively). Conclusion: F1616Y may be associated with SSS.

화이트헤드의 유기체 철학과 쿼크

임진아 ( Jin A Yim ) 한국화이트헤드학회 2013 화이트헤드 연구 Vol.26 No.-

궁극적 물질은 무엇이며, 그 물질의 성질에 대해 연구하는 분야는 물리학 중에서도 소립자물리학의 연구영역이다. 소립자물리학이 궁극적 요소에 대한 사유에는 궁극적 요소는 물질이며, 파동이 아닌 입자적 특성을 가진 그 무엇이 아니겠는가 하는 가정이 전제되어 있다. 20세기 초반, 플랑크의 에너지 양자가설로 인해 촉발된 물리학의 혁명은 물리학이 연구하는 대상인 물질 그 자체에 대한 관점의 전환을 요구했다. 파동과 입자는 양립할 수 없는 물질의 양태이다. 그러나 물질의 이중성으로 잘 알려져 있듯이 파동은 입자의 성질을, 입자는 파동의 성질을 가지고 있다는 것이 드러났다. 또한 극미의 세계에서는 매우 많은 숫자의 소립자가 발견되었으나, 현재 물리학자들은 양성자와 중성자와 같은 핵자를 만드는 근본적인 물질로 간주하는 쿼크 및 전자를 이 세계를 구성하는 가장 근원적인 입자들로 생각 한다.이 글은 두 가지의 목적을 가지고 있다. 첫 번째, 20세기에 양자역학에서 시작된 물질의 이중성으로부터 쿼크가 가진 성질을 통해 전통적인 물질관에 대해 비판하고자 한다. 두 번째, 현재까지도 명쾌한 해답이 제시되지 않고 있는 물질의 이중성 및 쿼크의 실체성에 대한 의문을 화이트헤드의 유기체철학의 관점으로 고찰해보고자 한다. The ultimate matter is what , and an field to study for the property of the material is study are of the particle physics. The ultimate element is matter in the reason for the ultimate element particle physics, And it is to a prerequisite a home doing whether it is the what with the property of the particle that is not wave motion. The prompted physical revolution required the change over of the point of view for material itself which was the object which physics studied(grand prix) by Energy Quantum hypothesis of initial, Planck in the 20th century. Wave motion and a particle are appearance of the materials which they cannot balance. However, it appeared in the wave motion that the particle had a nature of the wave motion with the nature of the particle to be known for the duality of the material well. In addition, the elementary particle of a great many numbers was discovered in the particle physical world, but current physicists think about quark to consider to be a basic material making the nuclear particle which seems to be a neutron with a positive child and an electron with root-like particles to frame this world. This essay consider as two kinds of purposes. It thinks first to think to criticize it for outlook on traditional material through the nature that quark has from the duality of the material which began in quantum mechanics in the 20th century. It thinks to consider a shrug for the second, duality of the material that the answer that is clear to date is not shown and substantiality of the quark at a point of view of the organized body philosophy of A. N. Whitehead.

간호대생과 의대생의 간호사 이미지 비교

구민진,김수영,방정민,서아영,양희진,윤소람,이윤재,이재은,이지연,정윤경,최수정 이화여자대학교 간호과학대학 2014 이화간호학회지 Vol.- No.48

Purpose: This study aims to analyze the difference in the perception that nursing students and medical students have regarding the image of nurses. Method: The sampling group for this study was made up of 111 nursing students and 117 medicine students, conducted from the 19th August 2013 to 3rd September 2013. The tool used for this study is the “Nurse Image Scale”. The data is analyzed using the SPSS 21.0 program, technical stats, t-test and ANOVA with Scheffe test. Result: There was a notable difference in the results(t=6.94, p<001), with the average image perception score of nurses at 3.84±0.34 amongst nursing students being higher than the 3.50±0.38 amongst Medicine students. The average score of the 4 areas tested, “Qualification of a Nurse”, “Role of a Nurse”, “Social Participation of a Nurse” and “Interpersonal Skills of a Nurse” were all marked higher by the nursing students than the medicine students. The average score became notably higher as the period of practice became shorter with nursing students (F=4.21, p=.043). Furthermore, the average score for the “Qualification of a Nurse” was notably higher as the period of practice became shorter (F=3.98, p=.049). Medical students gave an average score for the “Qualification of a Nurse”(F=3.72, p=.027) and the “Interpersonal Skills of a Nurse”(F=4.11, p=.019) which was relative to the development of a nurse’s image, while the average score for the “Role of a Nurse” was notably higher with a longer period of practice(F=6.65, p=.011). Conclusion: The results of this study suggest that the image perception of a nurse can vary depending on the experience in period of practice. Therefore, together with this study conducted with nursing students and medicine students, there is a need for further studies conducted on image perception of nurses with various experience in period of practice.

$n$-weak amenability and strong double limit property

A.R. Medghalchi,T. Yazdanpanah 대한수학회 2005 대한수학회보 Vol.42 No.2

Let {mathcal A} be a Banach algebra, we say that {mathcalA}has the strongly double limit property (SDLP) if for each boundednet (a_{alpha}) in {mathcal A} and each bounded net(a^*_{beta}) in {mathcal A}^*, lim_{alpha}lim_{beta}leftlangle a_{alpha} , a^*_{beta}rightrangle = lim_{beta} lim_{alpha} leftlangle a_{alpha}, a^*_{beta} rightrangle whenever both iterated limits exist. In this paperamong other results we show that if {mathcal A} has the SDLP and{mathcal A}^{**} is (n-2)-weakly amenable, then {mathcal A} isn-weakly amenable. In particular, it is shown that if {mathcalA}^{**} is weakly amenable and {mathcal A} has the SDLP, then ${\mathcal A}$ is weakly amenable

A spick-and-span approach to the immobilization of horseradish peroxidase on Au nanospheres incorporated with a methionine/graphene biomatrix for the determination of endocrine disruptor bisphenol A

Vilian, A.T. Ezhil,Giribabu, Krishnan,Choe, Sang Rak,Muruganantham, Rethinasabapathy,Lee, Hoomin,Roh, Changhyun,Huh, Yun Suk,Han, Young-Kyu Elsevier Sequoia 2017 Sensors and actuators. B Chemical Vol.251 No.-

<P><B>Abstract</B></P> <P>In the present study, we employ a straightforward, benign strategy to prepare thiol-functionalized reduced graphene oxide (S-RGO) using methionine as the sulphur source and reducing agent. The immobilization of horseradish peroxidase (HRP) over the AuNPs/S-RGO was developed by incorporating AuNPs on the S-RGO surface. The fabricated HRP/AuNPs/S-RGO electrode exhibits a remarkable decrease in the overpotential and a significantly increased oxidation peak current of bisphenol A (BPA) compared with the bare glassy carbon electrode (GCE) and AuNPs/S-RGO electrode. The biosensor shows an excellent amperometric analytical performance with a low detection limit of 2.6×10<SUP>−12</SUP> M and a linear range from 2.0×10<SUP>−11</SUP> to 1.18×10<SUP>−9</SUP> M, with the response time <2s for BPA. From the results, the apparent Michaelis-Menten constant was calculated as 8.14nM. The HRP/AuNPs/S-RGO biosensor exhibited faster response, adequate storage stability, inexpensive, simple fabrication with disposability, satisfactory reproducibility and repeatability, and outstanding selectivity. Finally, the constructed biosensor was utilized successfully for detecting BPA in tomato juice and milk samples with acceptable results.</P> <P><B>Highlights</B></P> <P> <UL> <LI> HRP/AuNPs/S-RGO biocomposite has been prepared to determine bisphenol A. </LI> <LI> The AuNPs/S-RGO is a promising platform for HRP immobilization. </LI> <LI> The biosensor exhibits excellent stability, reproducibility and high selectivity. </LI> <LI> It exhibits LOD of 2.6×10<SUP>−12</SUP> in a wide linear range from 2.0×10<SUP>−11</SUP> to 1.18×10<SUP>−9</SUP> M. </LI> <LI> It detects bisphenol A in real samples such as tomato juice and milk. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

지역사회간호사업의 내용 및 평가에 관한 연구 : 일부 저소득층 지역사회를 중심으로

방매륜,한영란 이화여자대학교 간호과학연구소 1990 Health & Nursing Vol.2 No.-

All people aspire to a life of health from birth to death. Health is both a right and a responsibility. Health as a human right is expressed in the WHO stated purpose of "Health for All by the Year 2000." In Korea an unequal distribution of health services is related to the ever increasing cost of medical care due to the professionalization of health services, the costly training and education of health care professionals and the soaring costs of medical equipment. It is now generally accepted that the health care wanted and needed by the people is first and foremost the activation and expansion of equally accessable primary health care. This study was carried out by the nursing staff of the Community Health Nursing Clinic of the Social Welfare Centre of Ewha Womans Uni versity. The research problems included the identification of principles from theories supportive of community health nursing services and of criteria from these theories useful for the evaluation of the services and as objectives to guide future services : the description of the i development of the services with a selected community group over a three year period : the evaluation of those services using the criteria developed from the theories and the recommendation of guide - lines for the planning and practice of these and similar community health nursing services. The purpose was to develop a community health nursing model for the expansion and activation of primary health care. The population was a group of women living with their families in a crowded poor area in one district in the Mapho area of Seoul. The women had organized as a mothers association to strength - en their autonomy and decision-making power. The study describes the process whereby the nurses and women worked together from 1987 to 1989 and how the women changed to become active and critical participants in decision making about their health and the nursing services. A theoretical framework was developed to support and guide the practice using Rogers Science of Unitary Human Beings, concepts of Primary Health Care from Health for All by the Year 2000, the health challenges, promotion mechanisms and implementation strategies of the Framework for Health Promotion of the Canadian Ministry of Health and Welfare and principles from the Ottawa Charter for Health Promotion. Four criteria for evaluation of the nursing services were developed from the concepts and principles of the theories. They were : 1. that a nursing theory worthy of providing a world view of the complexity of interrelationships between the human and environmental fields involved in community health nursing - the Science of Unitary Human Beings suplemented by concepts from Primary Health Care and Health Promotion-be used to direct services ; 2. that the people of a community participate in full partnership with service agencies in making decisions regarding the planning, provision and evaluation of their health care ; 3. that both the nurses and people be empowered , and 4. that the community health services be strengthened. Evaluation using the criteria demonstrated the effectiveness of the services in achieving the valued objectives of theory-directed care, empowerment and participation with this population, but relative ineffectiveness in achieving change in official public health policies which would strengthen services or promote a more healthy environment. The four criteria were proposed for use in building more concrete objectives for community health nursing services and the process as helpful for developing future services.

A characterization of normal subgroups via -closed sets

A. Badawi 장전수학회 2012 Advanced Studies in Contemporary Mathematics Vol.22 No.2

Let (G, *) be a semigroup, D ⊆ G, and n ≥ 2 be an integer. We say that (D, *) is an n-closed subset of G if a_1 * ... * a_n ∈ D for every a_1, ..., a_n ∈ D. Hence every closed set is a 2-closed set. The concept of n-closed sets arise in so many natural examples. For example, let D be the set of all odd integers, then (D, +) is a 3-closed subset of (Z, +) that is not a 2-closed subset of (Z, +). If K = {1, 4, 7, 10, ...} , then (K, +) is a 4-closed subset of (Z, +) that is not an n-closed subset of (Z, +) for n = 2, 3. In this paper, we show that if (H, *) is a subgroup of a group (G, *) such that [H : G] = n < ∞,then H is a normal subgroup of G if and only if every left coset of H is an n + 1-closed subset of G.

A microfluidic electrochemical aptasensor for enrichment and detection of bisphenol A.

Kashefi-Kheyrabadi, Leila,Kim, Junmoo,Gwak, Hogyeong,Hyun, Kyung-A.,Bae, Nam Ho,Lee, Seok Jae,Jung, Hyo-Il Elsevier 2018 Biosensors & bioelectronics Vol.117 No.-

<P><B>Abstract</B></P> <P>Bisphenol A (BPA) is an organic monomer used to make common consumer goods such as plastic containers, sports equipment, and cosmetics which are heavily produced worldwide. A growing interest has been drawn to general public as BPA is one of the major endocrine disrupting chemicals threating human health. To date, numerous BPA sensors have been attempted to be developed but important challenges still remained such as limited linearity range, easy to use, and long term response time. To address the present issues, a microfluidic channel should be integrated into an electrochemical aptasensor and it is called Geometrically Activated Surface Interaction (GASI) chip. The vigorous generation of the micro-vortex in the GASI fluidic chamber provides the high collision chances between BPA and anti-BPA aptamer (BPAPT) and consequently more BPA molecules can be captured on the aptasensor surface, which finally results in high sensitivity of the aptasensor. To construct the integrated aptasensor, a miniaturized gold electrode is fabricated using shadow mask and e-beam evaporation process. Afterward, BPAPT is immobilized on a nanostructured gold electrode via thiol chemistry, and other terminus of the aptamer is labeled with a ferrocene (Fc) redox probe. Then, the microfluidic channel is mounted over the miniaturized gold electrode to introduce and enrich BPA to the aptasensor. Upon the specific interaction between BPA and its aptamer, configuration of aptamer is changed so that Fc tag approaches to the electrode surface and direct oxidation signal of Fc and BPA are followed as analytical signals. The unique microfluidic integrated electrochemical aptasensor delivers a wide linear dynamic range over 5 × 10<SUP>–12</SUP> to 1 × 10<SUP>−9</SUP> M, with a limit of detection 2 × 10<SUP>–13</SUP> M. This aptasensor provides a precise platform for simple, selective and more importantly rapid detection of BPA. Such kind of sensing platforms can serve as a fertile ground for designing miniaturized portable sensors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> GASI generates micro-vortex resulting in enhanced capture of BPA and subsequently enhanced sensitivity of the aptasensor. </LI> <LI> The aptasensor has low LOD, wide linear dynamic range and good response time compared to conventional aptasensors. </LI> </UL> </P>

Second preduals of tridual von Neumann algebras are geometrically unique

A. I. Shtern 장전수학회 2008 Advanced Studies in Contemporary Mathematics Vol.17 No.1

The main result of the note claims that, if a von Neumann algebra Ais the third dual to some Banach space and thus, as is known, is the bidual of somevon Neumann algebra A, then the natural homomorphic projection of A onto theweakly closed two-sided ideal in A isomorphic to A (the kernel of this homomorphismcoincides with that of the canonical projection of A onto A dual to the canonicalembedding of the predual of A in the predual of A) is dened uniquely by A. Thus,the von Neumann algebra A, the image of the above projection, is not only uniquelydened (up to isomorphism) by its enveloping von Neumann algebra A but also theimage of the \canonical normal embedding" of A in A (onto the image of the aboveprojection) is uniquely dened, i.e., this weakly closed two-sided ideal in A isomorphicto A is uniquely dened geometrically. This enables us to rene some known resultson predual spaces of von Neumann algebras.

Lebesgue’s criterion for the Riemann integration with respect to a state on a separable unital C*- algebra

A. I. Shtern 장전수학회 2016 Advanced Studies in Contemporary Mathematics Vol.26 No.2

In the paper “Semicontinuous envelopes, Riemann integral, and uniform distribution in C*-algebras” (Funct. Anal. Appl. 29 (4), 268– 275 (1995)(1996)), the author had constructed the Riemann integral with respect to a state on a separable unital C*-algebra A. In particular, the semicontinuous hulls of elements of the enveloping von Neumann algebra A of A were introduced and studied and, for a given state ! on A, a class of selfadjoint elements of the algebra A that are Riemann integrable with respect to the state ω were introduced. It was also proved that this class is the self-adjoint part of a C*-algebra, and relations to the uniform distribution of states of the C*-algebra A with respect to the state ω were indicated. In the present note, we supplement these results by a version of Lebesgue’s criterion for the Riemann integrability of self-adjoint elements of A.